Table S6. Combinations / pairs of nodes from linear regression ... - PLOS

Table S6. Combinations / pairs of nodes from linear regression analysis improve stratification of FLT3-ITD and WT samples in Study 1. Node 1 Staurosporine & ZVAD→c-Caspase-8 | Fold IL-6→p-Stat5 | Total Staurosporine & ZVAD→c-Caspase-8 | Fold Staurosporine & ZVAD→c-Caspase-8 | Fold Staurosporine & ZVAD→c-Caspase-8 | Fold p-Stat5 | Basal Staurosporine→c-Caspase-8 | Total Staurosporine & ZVAD→c-Caspase-3 | Total Staurosporine & ZVAD→c-Caspase-8 | Fold IL-27→p-Stat5 | Total H2O2 & SCF→p-Erk | Fold Staurosporine→c-Caspase-8 | Total SCF→p-Stat5 | Total M-CSF→p-Stat5 | Total Staurosporine→c-Caspase-8 | Total FLT3L→p-S6 | Total IL-27→p-Stat5 | Total IL-6→p-Stat5 | Total Staurosporine & ZVAD→c-Caspase-3 | Fold IL-6→p-Stat5 | Total H2O2 & SCF→p-Erk | Fold Staurosporine & ZVAD→c-Caspase-8 | Fold Staurosporine & ZVAD→c-Caspase-3 | Total SCF→p-Stat5 | Total Staurosporine→c-Caspase-8 | Fold M-CSF→p-Stat5 | Total SCF→p-Stat5 | Total IGF-1→p-Stat5 | Total IL-10→p-Stat5 | Total Staurosporine→c-Caspase-8 | Total Staurosporine→c-Caspase-8 | Total H2O2 & SCF→p-Erk | Fold EPO→p-Stat5 | Total H2O2 & SCF→p-Erk | Fold

Node 2 SCF→p-Stat5 | Total FLT3L→p-S6 | Total M-CSF→p-Stat5 | Total IGF-1→p-Stat5 | Total H2O2→p-Stat5 | Fold FLT3L→p-S6 | Total IL-27→p-Erk | Total FLT3L→p-CREB | Total p-Stat5 | Basal Staurosporine→c-Caspase-8 | Fold Etoposide & ZVAD→p-Chk2+, c-PARP + | Quad TNFα→p-NFkB-p65 | Fold Staurosporine→c-Caspase-8 | Fold Staurosporine→c-Caspase-8 | Fold FLT3L→p-S6 | Total IFNγ→p-Stat5 | Total FLT3L→p-S6 | Total Etoposide & ZVAD→p-Chk2+, c-PARP - | Quad H2O2→p-Stat5 | Fold Etoposide & ZVAD→c-PARP | Total Etoposide & ZVAD→p-Chk2+, c-PARP - | Quad p-Stat5 | Total TNFα→p-NFkB-p65 | Fold p-SLP-76 | Total ABCG2 | PercentPos FLT3L→p-S6 | Total IL-27→p-Erk | Total FLT3L→p-S6 | Total FLT3L→p-S6 | Fold Etoposide & ZVAD→c-PARP | Total Etoposide→c-PARP | Total G-CSF→p-S6 | Total H2O2 & SCF→p-Erk | Total

Num WTs/ITDs 15 17 15 15 14 17 15 15 15 15 14 15 15 15 15 17 17 14 14 14 14 15 15 13 14 17 17 17 17 14 14 14

/ / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / /

AUC Model

AUC Node1 Alone

14 15 14 14 14 15 14 14 14 14 14 14 14 14 14 15 15 14 14 14 14 14 14 14 13 15 15 15 15 14 14 14

0.99 0.98 0.98 0.98 0.97 0.97 0.97 0.97 0.97 0.97 0.97 0.97 0.97 0.97 0.97 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96

0.90 0.84 0.90 0.90 0.89 0.85 0.87 0.89 0.90 0.83 0.91 0.87 0.85 0.84 0.87 0.80 0.85 0.79 0.85 0.79 0.91 0.90 0.89 0.84 0.85 0.85 0.87 0.85 0.87 0.86 0.86 0.91

AUC Node2 Alone 0.85 0.80 0.84 0.85 0.84 0.80 0.71 0.81 0.83 0.85 0.77 0.69 0.85 0.85 0.80 0.77 0.80 0.78 0.84 0.80 0.78 0.88 0.69 0.76 0.71 0.80 0.71 0.80 0.72 0.80 0.82 0.65

Node1 p- Node2 pValue Value 0.048 0.028 0.032 0.047 0.032 0.029 0.014 0.039 0.040 0.036 0.035 0.008 0.030 0.033 0.015 0.014 0.014 0.027 0.028 0.028 0.027 0.037 0.012 0.013 0.043 0.014 0.006 0.017 0.042 0.024 0.025 0.026

0.044 0.024 0.040 0.047 0.046 0.030 0.021 0.021 0.044 0.049 0.044 0.022 0.028 0.030 0.034 0.017 0.024 0.018 0.022 0.013 0.037 0.037 0.043 0.018 0.032 0.026 0.028 0.034 0.045 0.031 0.037 0.039

14

/

14

0.96

0.85

0.72

0.040

0.035

Staurosporine→c-Caspase-8 | Total Staurosporine→c-Caspase-8 | Total Staurosporine→c-Caspase-8 | Total p-Stat5 | Basal Staurosporine & ZVAD→c-Caspase-8 | Fold Staurosporine & ZVAD→c-Caspase-8 | Fold Staurosporine→c-Caspase-8 | Fold

SCF→p-CREB | Total Etoposide→c-Caspase-3 | Total CD40L→p-p38 | Total IL-6→p-CREB | Fold Staurosporine→c-Caspase-8 | Fold EPO→p-Stat5 | Total IL-10→p-Stat5 | Total IFNα→p-Stat5 | Total

14 12 15 15 15 15 15 15

/ / / / / / / /

14 14 14 14 14 14 14 14

0.96 0.96 0.96 0.96 0.96 0.96 0.96 0.96

0.91 0.86 0.87 0.87 0.83 0.90 0.90 0.85

0.77 0.82 0.67 0.73 0.85 0.85 0.86 0.78

0.032 0.036 0.016 0.010 0.037 0.031 0.022 0.032

0.042 0.049 0.032 0.035 0.042 0.045 0.047 0.050

p-Stat5 | Basal

M-CSF→p-S6 | Fold

17

/

15

0.96

0.89

0.77

0.013

0.032

M-CSF→p-Stat5 | Total IGF-1→p-Stat5 | Total IL-10→p-Stat5 | Total Staurosporine & ZVAD→c-Caspase-3 | Total

p-SLP-76 | Basal p-SLP-76 | Basal Etoposide→c-PARP | Fold H2O2→p-Stat5 | Fold

13 13 14 14

/ / / /

14 14 14 14

0.96 0.96 0.95 0.95

0.82 0.85 0.86 0.88

0.76 0.76 0.83 0.84

0.013 0.020 0.022 0.014

0.017 0.025 0.019 0.023

IL-27→p-Stat5 | Total

H2O2 & SCF→p-Erk | Total

14

/

14

0.95

0.82

0.72

0.009

0.023

IL-10→p-Stat5 | Total IL-10→p-Stat5 | Total Staurosporine→c-Caspase-8 | Total H2O2 & SCF→p-Erk | Fold

H2O2 & SCF→p-Erk | Total

14 14 14 14 17 17 12

/ / / / / / /

14 14 14 14 15 15 14

0.95 0.95 0.95 0.95 0.95 0.95 0.95

0.86 0.86 0.86 0.91 0.87 0.87 0.85

0.72 0.80 0.83 0.74 0.80 0.77 0.82

0.007 0.029 0.019 0.016 0.011 0.012 0.021

0.029 0.034 0.040 0.042 0.033 0.036 0.039

SCF→p-Stat5 | Total EPO→p-Stat5 | Total SCF→p-Stat5 | Total

Etoposide & ZVAD→c-PARP | Total Etoposide→c-PARP | Fold Etoposide & ZVAD→p-Chk2-, c-PARP + | Quad FLT3L→p-S6 | Total M-CSF→p-S6 | Fold Etoposide→c-Caspase-3 | Total

Filtered on AUC Model > 0.95 , p