1
Engineering Chlorpyrifos-Degrading Stenotrophomonas sp. YC-1 for Heavy Metal
2
Accumulation and Enhanced Chlorpyrifos Degradation
3
Ruihua Liu,1 Hong Jiang,2 Ping Xu,3 Chuanling Qiao,2 Qixing Zhou,4 Chao Yang1*
4 5 6
1
7
Nankai University, Tianjin 300071, China
8
2
9
Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences,
State Key Laboratory of Integrated Management of Pest Insects and Rodents,
10
3
11
Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
12
4
13
Education, College of Environmental Science and Engineering, Nankai University,
14
Tianjin 300071, China
State Key Laboratory of Microbial Metabolism, and School of Life Sciences &
Key Laboratory of Pollution Processes and Environmental Criteria for Ministry of
15 16
*Corresponding author:
17
Chao Yang (Tel./fax: 86-22-23503866; E-mail:
[email protected])
18 19 20 21 22 23 24 25
1
Western
blot
analysis
for
subcellular
localization
of
INPNC–EC20
in
Stenotrophomonas sp. YC-1 carrying pVINPEC20. Lane 1, whole-cell lysates; lane 2, negative control (YC-1/pVLT33); lane 3, soluble fraction; lane 4, outer membrane fraction. INPNC antiserum was used at a 1:1000 dilution.
'1$0$1)LOH , ?'1$0$1B7UDQVODWLRQVHT Translation of DNAMAN1(1-1023) 䗮⫼FRGH 7RWDODPLQRDFLGQXPEHU 0: 0D[25)VWDUWVDW$$SRV PD\EH'1$SRV IRU$$ EDVHV 0: $7*$$7$7&*$&$$$*&*77**7$&7*&*7$&&7*7*&$$$7$$&$7**&&*$7&$77*& 01,'.$/9/57&$110$'+& **&&77$7$7**&&&*&&7&&**&$&**7**$$7&&$$$7$&7**&$*7&$$&&$**&** */,:3$6*79(6.
