Cluster 27 - Tremellomycetes. FR682342 Cryptococcus carnescens. AB105432. FR682224 Cryptococcus carnescens. EU149786. FR682222 Cryptococcus ...
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Table S7. Phylogenetic description, nearest database relative and frequency of detection of fungal molecular OTUs and isolated strains recovered from dust and water damaged building materials. Phylotype name
BF-OTU468 BF-OTU576 BF-OTU418 BF-OTU456 BF-OTU408 BF-OTU410 BF-OTU695 BF132 BF-OTU511 BF133 BF-OTU698 BF-OTU438 BF-OTU597 BF-OTU586 BF-OTU469 BF-OTU700 BF-OTU585 BF-OTU589 BF-OTU612 BF-OTU496 BF-OTU582 BF-OTU630 BF-OTU676 BF-OTU697 BF-OTU687 BF-OTU686 BF-OTU521 BF-OTU455 BF-OTU443 BF-OTU462 BF-OTU419 BF-OTU484 BF-OTU541
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession Cluster* 1 - Eurotiomycetes FR682211 Ascomycete sp. AM292201 FR682319 Capronia villosa AF050261 FR682161 Capronia villosa AF050261 FR682199 Cladonia furcata EU266080 FR682151 Cladophialophora minutissima EF016382 FR682153 Cladophialophora minutissima EF016382 Cladophialophora sp. FR682438 EF016382 FR718472 Exophiala xenobiotica DQ182590 FR682254 Fungal sp. AY843097 FR718473 Rhinocladiella atrovirens AY618683 FR682441 Uncultured fungus AJ582963 FR682181 Uncultured fungus DQ309126 FR682340 Uncultured fungus AM260819 Cluster 2 - Eurotiomycetes FR682329 No full length match FR682212 No match FR682443 Phialocephala fluminis AF486124 FR682328 Phialophora sessilis AY857542 FR682332 Uncultured fungus AJ582963 Cluster 3 - Unknown FR682355 Fungal sp. AY843052 FR682239 Leaf litter ascomycete AF502889 FR682325 Phaeococcomyces chersonesos AJ507323 FR682373 Phaeococcomyces chersonesos AJ507323 FR682419 Phaeococcomyces chersonesos AJ507323 FR682440 Phialocephala fluminis AF486124 FR682430 Uncultured Peltigerales FJ554149 Cluster 4 - Unknown Verrucaria sp. FR682429 FJ664883 FR682264 Capronia villosa AF050261 Cluster 5 - Dothideomycetes FR682198 Botryosphaeria dothidea DQ008327 FR682186 Fungal sp. AY843079 FR682205 Fungal sp. AY843079 FR682162 Mycocalicium subtile AF225440 Cluster 6 - Dothideomycetes FR682227 Alternaria citri AY154705 FR682284 Bipolaris sorokiniana EF187908
Sim %
87.0 84.0 84.0 98.0 81.0 99.3 92.0 100.0 92.7 98.9 83.3 83.5 96.5
Final annotation **
UA Eu Eu Lc § Eu Cladophialophora minutissima Eu § Exophiala sp. UA Rhinocladiella atrovirens Eu Eu Eu §
In1a 10 1
1 1 2 1 2 2
82.1 99.5 83.1 99.6 96.2 90.3 90.3 90.9 86.1 98.6
Le § Eu Eu Eu Eu § Le Lc §
82.0 82.0
Eu Eu
C C 1 1
100.0 99.6
0
0
0
0
1
0
1 3 1
0
1 1
0
79.2 81.8 80.8 76.3
1 2
1 UF UF Le Phialophora sessilis Eu
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 2 0 1 0 2 0 1 0
2
0
1
1
3
0
1 1 2 1
0
2 1 1 1
Do UA UA Eu Alternaria citri Bipolaris sorokiniana
1
0
0
1
0
0
8 2 1 4 1 1 1
0
1 0
0
0
0
1
0
2
1
1 2 2 2
0
0
0
0
0
0
0
0
1 1
1 1
0
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU662 BF-OTU440 BF-OTU692 BF-OTU610 BF-OTU628 BF-OTU409 BF-OTU454 BF-OTU721 BF-OTU716 BF-OTU552 BF-OTU724 BF-OTU719 BF-OTU717, BF043 BF-OTU704 BF-OTU722 BF051 BF-OTU632 BF030 BF-OTU710 BF-OTU711 BF-OTU713 BF-OTU709 BF-OTU712 BF-OTU649 BF-OTU648 BF-OTU472 BF001 BF029 BF-OTU532, BF167 BF027 BF-OTU679, BF115 BF006
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession FR682405 Phaeococcomyces nigricans AF050278 Cluster 7 - Unknown FR682183 Mycosphaerella swartii DQ923536 Cluster 8 - Leotiomycetes FR682435 Mollisia fusca AY259138 FR682353 Melanized limestone ascomycete AY559378 Cluster 9 - Unknown FR682371 Uncultured ascomycete isolate AY969391 Cluster 10 - Unknown FR682152 Colletogloeopsis blakelyi DQ923526 Cluster 11 - Eurotiomycetes FR682197 Phaeococcomyces nigricans AY843154 Cluster 12 - Eurotiomycetes/Leotiomycetes FR682463 Aspergillus conicus AY373865 FR682458 Aspergillus conicus EF652039 FR682295 Aspergillus conicus EF652039 FR682466 Aspergillus conicus EF652039 FR682461 Aspergillus conicus AY373865 FR682459, Aspergillus conicus EF652039 FR718462 FR682447 Aspergillus conicus AY373864 FR682464 Aspergillus conicus EF652039 FR718464 Aspergillus nidulans AF455505 FR682375 Aspergillus ochraceus AY609210 FR718457 Aspergillus sydowii AY373868 FR682453 Eupenicillium javanicum AY373921 FR682454 Eupenicillium javanicum U18358 FR682456 Eupenicillium javanicum U18358 FR682452 Eupenicillium javanicum U18358 FR682455 Eupenicillium javanicum U18358 FR682392 Eurotium amstelodami AF455464 FR682391 Eurotium niveoglaucum AF459728 FR682215 Geomyces pannorum DQ189229 FR718449 Paecilomyces divaricatus AY7373851 FR718456 Paecilomyces variotii AF033395 FR682275, Penicillium brevicompactum AY373898 FR718486 FR718455 Penicillium canescens AF033493 FR682422, Penicillium chrysogenum AF034449 FR718468 FR718452 Penicillium citreonigrum AF033456
Sim %
Final annotation ** In1a
80.7
Eu §
84.0
Do
98.8 92.3
Mollisia fusca Do
1 1 0
93.4
Le
82.7
Do §
99.4
Phaeococcomyces nigricans
96.8 98.0 98.0 98.9 99.0 99.0
Aspergillus sp. Aspergillus conicus Aspergillus conicus Aspergillus conicus Aspergillus conicus Aspergillus conicus
99.0 97.8 100.0 100.0 99.6 89.0 89.9 90.1 90.2 90.3 99.4 100.0 99.8 99.8 100.0 99,8-100
Aspergillus conicus Aspergillus sp. Aspergillus nidulans Aspergillus ochraceus Aspergillus sydowii Eu Eu Eu Eu Eu Eurotium sp. Eurotium sp. Geomyces pannorum Paecilomyces divaricatus Paecilomyces variotii Penicillium sp.
99.8 99,8-100
Penicillium sp. Penicillium sp.
100.0
Penicillium citreonigrum
2
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 2 0 0 0 0 0 0 0 0 0
0
0
0
1 1
0
0
0
0
0
0
0
0
3 3 1 1 3
0
2 2 1 1 2
0
0
0
0
1 1 1 0
0
0
0
0
0
0
49
5
59
8
0
1
1
0 0
1
0
134 1 2 28 1 44 C, 1
1
0
0 0
1 1 C 1 C 1 1 23 3 23 1 1
1 1 C C 2
C
1
C C C, 1 C
C, 36
C, 5
46
1
C
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU651, BF25 BF-OTU624, BF037 BF-OTU608 BF-OTU587 BF026 BF-OTU723, BF005 BF042 BF-OTU602 BF-OTU416 BF-OTU505 BF-OTU467 BF-OTU504 BF-OTU688 BF-OTU499 BF-OTU627 BF-OTU411 BF-OTU631 BF-OTU510 BF-OTU619 BF-OTU486 BF-OTU483 BF-OTU653 BF-OTU671 BF-OTU681 BF-OTU555 BF-OTU497, BF048 BF-OTU422 BF-OTU458 BF-OTU574 BF-OTU559 BF-OTU546 BF-OTU507 BF-OTU642
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession FR682394, Penicillium commune AF236103 FR718453 FR682367, Penicillium corylophilum AF034457 FR718459 FR682351 Penicillium glabrum AF033407 FR682330 Penicillium roquefortii AY373929 Penicillium sp. FR718454 AB297800 Penicillium sp. FR682465, FJ379809 FR718451 FR718461 Penicillium spinulosum GU566252 FR682345 Pleopsidium chlorophanum AY853384 Cluster 13 - Lecanoromycetes FR682159 Hypogymnia physodes AF058036 FR682248 Parmelia sulcata AF410839 FR682210 Parmeliopsis ambigua AF451764 Cluster 14 - Leotiomycetes FR682247 Lachnum bicolor AJ430394 FR682431 Meria laricis U92299 FR682242 Monilinia fructigena EF207427 FR682370 Mycocalicium victoriae AJ312123 FR682154 Sclerotinia sclerotiorum AF455523 Cluster 15 - Lecanoromycetes FR682374 Lecanora saligna AF189716 FR682253 Miriquidica garovaglii EU075538 Cluster 16 - Lecanoromycetes FR682362 Lecidella carpathica DQ534471 FR682229 Uncultured fungus AJ875379 Cluster 17 - Dothideomycetes Ampelomyces sp. FR682226 AF035783 Ascochyta sp. FR682396 AY305377 FR682414 Epicoccum nigrum AF455455 Fungal cont. of Leptosphaeria sp. FR682424 AJ002205 FR682298 Fungal sp. AM231348 FR682240, Leptosphaerulina chartarum DQ384571 FR718463 FR682165 Leptosphaerulina trifolii AY831558 FR682201 Lophium mytilinum EF596819 FR682317 Phaeosphaeria avenaria U77359 Phaeosphaeria sp. FR682302 AY345346 FR682289 Phaeosphaeriaceae sp. AY465459 FR682250 Phaeosphaeriopsis amblyspora AY188993 FR682385 Phoma exigua AJ608976
Sim %
Final annotation ** In1a
99.8 99,8-100
Penicillium sp. Penicillium corylophilum
99.8 99.8 92.0 99.1
Penicillium sp. Penicillium sp. Le Penicillium sp.
99.8 82.6
Penicillium sp. Lc
99.1 100.0 99.8
Hypogymnia physodes Parmelia sulcata Parmeliopsis ambigua
99.8 99.8 100.0 94.8 99.8
Lachnum bicolor Meria laricis Monilinia fructigena Eu § Le
85.0 85.8
Lc § Lc §
75.8 77.5
Lc UF §
100.0 99.6 99.8 80.8 99.6 99,5-100 99.8 99.6 95.5 93.0 96.5 89.7 99.0
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 C 13 9
Ampelomyces sp. Ascochyta sp. Epicoccum nigrum Pe Do § Leptosphaerulina chartarum Do Lophium mytilinum Phaeosphaeria sp. Do § Do § Do § Phoma exigua
3
C, 1
1
C
1 3 C C, 1 C 3 2
2
0
0
0
0
0
1 1 1
0
0
0
4
3
1
7
0
1 6 0
0
2 1 5
2 1
2 1 1 4 1
1
0
1 0
1 1
24
1 14 3
0
4 0
1 0
1 1 1
0
0
0
1
0
1 1
0
13
1
3
1 4
8
2
0
1 6
1 1
10
6
1 4
C
2
2 1 1 1 1 1
1 1
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU564 BF-OTU528, BF153 BF-OTU431, BF053 BF-OTU413 BF-OTU693 BF-OTU534 BF-OTU654, BF143 BF-OTU527, BF168 BF-OTU641 BF-OTU592 BF-OTU494 BF-OTU708 BF-OTU459 BF-OTU572 BF-OTU616 BF-OTU415 BF-OTU609 BF-OTU498 BF-OTU519 BF-OTU463 BF-OTU476 BF-OTU426 BF-OTU573, BF125 BF-OTU417 BF-OTU445 BF-OTU677 BF-OTU406 BF-OTU690 BF-OTU430 BF-OTU513 BF-OTU407 BF-OTU622 BF-OTU638 BF-OTU427 BF-OTU412
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession FR682307 Phoma exigua AJ608976 FR682271, Phoma herbarum AY293800 FR718481 FR682174, Phoma herbarum AY337712 FR718466 FR682156 Phoma macrostoma DQ474114 Phoma sp. FR682436 EF423518 Phoma sp. FR682277 DQ344033 Phoma sp. FR682397, AY465466 FR718477 Phoma sp. FR682270, AF218789 FR718487 FR682384 Pleiochaeta setosa EU167563 FR682335 Uncultured fungus isolate DQ093786 FR682237 Uncultured soil fungus clone DQ420828 Cluster 18 - Dothideomycetes FR682451 Amorphotheca resinae FR682202 Ascomycete sp. EF373580 FR682315 Aureobasidium pullulans DQ640766 FR682359 Aureobasidium pullulans AY213639 FR682158 Aureobasidium pullulans AM160630 FR682352 Dothichiza pityophila AJ244242 FR682241 Ectomycorrhizal isolate AY345353 FR682262 Elsinoe ampelina AY826762 FR682206 Fungal sp. AY843105 Hormonema aff. prunorum FR682219 AY188367 FR682169 Hormonema carpetanum AY616200 FR682316, Hormonema dematioides AJ278927 FR718470 FR682160 Hypocenomyce scalaris DQ782852 FR682188 Micarea lapillicola AY756479 FR682420 Mycosphaerella africana AY626981 FR682149 Mycosphaerella parva AY626980 Mycosphaerella sp. FR682433 DQ458904 FR682173 No full length match FR682256 Pringsheimia smilacis AJ244257 FR682150 Sawadaea tulasnei AB193361 FR682365 Sphaerulina eucalypti AY293060 FR682381 Trimmatostroma abietis AY128697 Cluster 19 - Dothideomycetes FR682170 Fungal sp. AY843079 FR682155 Fungal sp. AY843191
Sim %
Final annotation ** In1a
99.6 100.0
Phoma sp. Phoma herbarum
100.0
Phoma herbarum
1
99.8 99.0 99.4 99,5-100
Phoma sp. Phoma sp. Phoma sp. Phoma sp.
3
99,8-100
Phoma sp.
93.3 86.4 96.2
97.3 86.3 98.2 84.0 94.5
C 1
2 3 3
2
1
C
1
AsIs UF Pe
C 2 1
30 81.0 85.5 99.8 99.8 100.0 80.0 97.6 84.2 92.9 98.0 99.0 99,1-100
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 1 1 1 1 C
UF Do Aureobasidium pullulans Aureobasidium pullulans Aureobasidium pullulans Do Le Do UA § Hormonema sp. Do Hormonema dematioides
98.1 99.4 79.5 99.8
Lc Lc Mycosphaerella sp. Do Do § UF § Pringsheimia smilacis Sawadaea tulasnei Do § Trimmatostroma sp.
84.6 87.7
Do Do §
4
1 1 11
5
3
6
3 3
6
9
1
1
3
1
2
2
3
2
1
4
1
24
0
2 3 16 1
1 1 2 3 2 1
6
1
1
1
C
1 2 1 3 3 2 1 1 1 5 1 1
4
1 13
1
0
0
0
0
2 13 2
0
0
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU508 BF-OTU447 BF-OTU637 BF-OTU414 BF-OTU493 BF-OTU540 BF-OTU405, BF141 BF150 BF-OTU491 BF-OTU425, BF060 BF-OTU503, BF129 BF-OTU478 BF-OTU613 BF-OTU433 BF-OTU446 BF-OTU423 BF-OTU450 BF-OTU473 BF-OTU453 BF-OTU583 BF-OTU707 BF-OTU706 BF-OTU449 BF-OTU530 BF-OTU621 BF-OTU516 BF-OTU699 BF-OTU457 BF134 BF-OTU598 BF-OTU568 BF-OTU703 BF052 BF-OTU623 BF-OTU650
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession FR682251 Fungal sp. AY843115 FR682190 Fungal sp. AY843191 FR682380 Rhizoctonia bataticola DQ222241 FR682157 Unknown dothideomycete AY560007 Cluster 20 - Dothideomycetes FR682236 Cladosporium cladosporioides AY361968 FR682283 Cladosporium cladosporioides AF455525 FR682148, Cladosporium cladosporioides AF393691 FR718476 FR718479 Cladosporium cladosporioides DQ426533 FR682234 Cladosporium herbarum AY251078 FR682168, Cladosporium herbarum AF393712 FR718467 Cladosporium sp. FR682246, AJ300336 FR718471 FR682221 Cladosporium sphaerospermum AF455481 FR682356 Eudarluca caricis AY607017 FR682176 Fungal sp. AY843079 FR682189 Fungal sp. AY843182 FR682166 Phaeotheca fissurella AJ244255 FR682193 Phaeothecoidea proteae EU707898 FR682216 Roccella peruensis AJ634026 FR682196 Sarcinomyces crustaceus AJ244258 Cluster 21 - Dothideomycetes FR682326 Fusicladium fagi EU035431 FR682450 Mycosphaerella flexuosa DQ302958 FR682449 Mycosphaerella flexuosa DQ302958 FR682192 Venturia ditricha EU035456 FR682273 Venturia tremulae EU035475 Cluster 22 - Sordariomycetes FR682364 Cadophora luteo-olivacea GQ214536 FR682259 Chaenothecopsis haematopus AY795861 Helgardia sp. FR682442 AM262430 FR682200 Holwaya mucida DQ257357 Cadophora sp. FR718474 AY371513 FR682341 Mycocalicium victoriae AJ312123 FR682311 Mycocalicium victoriae AJ312123 FR682446 Mycocalicium victoriae AJ312123 Cluster 23 - Sordariomycetes FR718465 Acremonium alternatum AM176679 FR682366 Acremonium alternatum AM176679 FR682393 Acremonium alternatum U57674
Sim %
Final annotation ** In1a
98.6 99.0 76.0 100.0
Eu § Do § Ag Do
99.6 100.0 100.0
Cladosporium sp. Cladosporium sp. Cladosporium sp.
100.0 99.0 100.0
Cladosporium sp. Cladosporium sp. Cladosporium sp.
99,6-100
Cladosporium sp.
1 11 2 41
100.0 99.6 84.9 99.4 86.4 96.8 70.3 99.0
Cladosporium sp. Cladosporium sp. Lc Do § Do § Phaeothecoidea sp. Art Sarcinomyces crustaceus
99.8 83.4 83.4 99.4 98.6
Cladosporium nigrellum Do Do Venturia sp. Venturia sp. So Chaenothecopsis haematopus Helgardia sp. Le Le Eu Eu Eu
15
15
1 1
So So So
11
0
5
1 4
3
10
9
0
2 4
2 3
6 1
C
1
5
1
C 4
C
1 1
115
C 1 1
1 2 10 7 1 1 1
1 3
1
0
1
1
0
3
0
0
0
1 1
0
0
2
0
2 1
0
2 1 1 0
1 1
1
1 1 1 C 1 1 4
86.9 89.4 91.3
138 1
3
1 99.7 98.1 100.0 88.0 100.0 88.2 94.9 90.8
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 1
8
2
0
1 C
1
1 15
2
23 1
1
5
0
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU477 BF-OTU618 BF-OTU480 BF-OTU464 BF-OTU531 BF-OTU500 BF-OTU615 BF-OTU565 BF-OTU421 BF-OTU684 BF135 BF-OTU487 BF-OTU512 BF-OTU675 BF-OTU605 BF-OTU606 BF-OTU705, BF039 BF-OTU600 BF-OTU718 BF002 BF152 BF124 BF-OTU601 BF-OTU636 BF-OTU689 BF-OTU526 BF-OTU593 BF-OTU694 BF-OTU550 BF-OTU629 BF-OTU448 BF-OTU599 BF-OTU481 BF-OTU479 BF-OTU485 BF-OTU596 BF-OTU520
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession FR682220 Acremonium alternatum AM176679 FR682361 Acremonium cyanophagus DQ393594 Cephalosporium sp. FR682223 AM176722 FR682207 Chaetomium globosum DQ003217 FR682274 Colletotrichum linicola AB046609 Cryptodiaporthe cf. Hystrix FR682243 EU255027 FR682358 Cryptodiaporthe salicella DQ323529 FR682308 Diatrypella pulvinata AJ302443 FR682164 Fusarium oxysporum AF538623 FR682427 Fusarium oxysporum DQ535184 FR718475 Lecythophora hoffmannii AY805566 FR682230 Microdochium nivale EF187912 FR682255 Myrothecium atroviride AJ302002 FR682418 No full length match Nodulisporium sp. FR682348 EF600033 FR682349 Paecilomyces major DQ243696 Phialophora sp. FR682448, AY618679 FR718460 Sordariomycete sp. FR682343 DQ227290 FR682460 Stilbella byssiseda AF335453 FR718450 Thielavia hyalocarpa AJ271583 FR718480 Trichoderma atroviride EF417482 FR718469 Trichoderma citrinoviride AJ230663 FR682344 Trichoderma harzianum AJ230664 FR682379 Trichoderma koningii AF218790 Cluster 24 - Taphrinomycetes FR682432 Taphrina ulmi AF492123 Cluster 25 - Saccharomycetes FR682269 Candida famata AM992913 FR682336 Candida zeylanoides AB278160 FR682437 Saccharomyces cerevisiae AY939814 FR682293 Saccharomyces cerevisiae D89886 FR682372 Saccharomyces cerevisiae U53879 Cluster 26 - Unknown FR682191 No match Cluster 27 - Tremellomycetes FR682342 Cryptococcus carnescens AB105432 FR682224 Cryptococcus carnescens EU149786 FR682222 Cryptococcus festucosus AY633979 FR682228 Cryptococcus foliicola AY557600 Cryptococcus sp. FR682339 DQ317359 FR682263 Cryptococcus tephrensis DQ000318
Sim %
Final annotation ** In1a
100.0 83.4 99.8 99.8 99.5 99.8 98.9 99.8 99.8 99,6-100 100.0 99.8 100.0 99.8 97.7 99,2-99,3 89.2 83.5 98.0 99.8 100.0 99.8 99.8
Acremonium sp. So So Chaetomium globosum Colletotrichum sp. Cryptodiaporthe sp. Cryptodiaporthe salicella Diatrypella pulvinata Fusarium sp. Fusarium oxysporum Lecythophora hoffmannii Microdochium nivale Myrothecium sp. UF § Nodulisporium sp. Paecilomyces sp. Phialophora sp.
1 1 1 1 1 3
1 13 C 1 1 1
Ta
99.8 99.3 99.3 99.8 99.6
Candida sp. Candida zeylanoides Saccharomyces cerevisiae Saccharomyces cerevisiae Saccharomyces cerevisiae
C, 5 2 1 C C C 1 0
0
0
0
0
3 3
0
3 2
0
0
Cryptococcus carnescens Cryptococcus carnescens Cryptococcus festucosus Cryptococcus sp. Cryptococcus sp. Cryptococcus sp.
6
1
1 1 73 1
0
0
0
0
1 46 25 0
11
5
0
2
2
1 1 1 1
UF
1 1 2
So So So Trichoderma sp. Trichoderma sp. Trichoderma sp. Trichoderma sp.
90.3
99.8 99.8 99.4 97.4 98.8 98.3
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 2 13 1 3
7 1 2
1 1 0 0
0 0
0
0
7 4 1
0
1 1 0 11 5
2
2
2
1 2
0 0
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU607 BF-OTU557 BF-OTU489 BF-OTU515 BF-OTU451 BF-OTU471 BF-OTU577 BF-OTU403 BF-OTU506 BF-OTU465 BF-OTU659 BF-OTU545 BF-OTU558 BF-OTU668 BF-OTU474 BF-OTU666 BF-OTU667 BF-OTU647 BF-OTU543 BF-OTU400 BF-OTU620 BF-OTU663 BF-OTU672 BF-OTU591 BF-OTU691 BF-OTU551 BF155 BF-OTU644 BF145 BF-OTU656 BF-OTU639 BF-OTU595 BF-OTU652 BF-OTU714 BF-OTU571
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession Cryptococcus sp. FR682350 DQ317359 FR682300 Cryptococcus tephrensis DQ000318 FR682232 Cryptococcus victoriae AF444469 FR682258 Cryptococcus victoriae AF444447 Cluster 28 - Tremellomycetes FR682194 Cryptococcus hungaricus AF272664 FR682214 Cryptococcus hungaricus AF444379 FR682320 Cryptococcus rajasthanensis AM262325 Cryptococcus sp. FR682146 AF444332 FR682249 Fellomyces fuzhouensis AF444401 FR682208 Tremella giraffa AF042453 FR682402 Tremellales sp. EF060541 FR682288 Uncultured basidiomycete AY969511 Cluster 29 - Agaricomycetes FR682301 Collybia dryophila AY521249 FR682411 Gymnopus dryophilus AY256690 FR682217 Mycena galericulata DQ404392 FR682409 Mycena luteopallens AB512313 Cluster 30 - Agaricomycetes FR682410 Armillaria ostoyae AY213552 FR682390 Cylindrobasidium evolvens AY618227 FR682286 Flammulina velutipes AF036928 FR682143 Pleurotus salmoneostramineus AY265843 FR682363 Stephanospora caroticolor AJ419224 Cluster 31 - Unknown Rhizoctonia sp. FR682406 AY927338 Cluster 32 - Agaricomycetes FR682415 Typhula variabilis AB267394 FR682334 Typhula variabilis AB267394 Cluster 33 - Microbotryomycetes FR682434 Antarctic yeast AY040664 FR682294 Leucosporidium scottii AM117830 FR718482 Rhodosporidium lusitaniae AY015430 FR682387 Rhodosporidium lusitaniae AJ853459 FR718478 Sporodiobolus salmonicolor AY015434 FR682399 Sporobolomyces jilinensis AY364838 FR682382 Sporobolomyces ruberrimus AY015436 Cluster 34 - Agaricomycetes FR682338 Armillaria novae-zelandiae AF394919 FR682395 Auriscalpium vulgare AF454430 FR682457 Eupenicillium javanicum U18358 FR682314 Rhizoctonia cerealis AF063019
Sim %
Final annotation ** In1a
98.2 97.9 98.8 99.4
Cryptococcus sp. Cryptococcus sp. Cryptococcus victoriae Cryptococcus victoriae
97.7 99.3 94.2 75.8 75.8 86.3 97.8 80.6
Cryptococcus sp. Dioszegia hungarica Tr Tr Tr Tr Tr Tr
98.7 99.6 99.7 89.5
Gymnopus sp. Gymnopus dryophilus Mycena galericulata Ag §
4 1 1
1 1 1 1 1 1
Armillaria ostoyae Ag Flammulina velutipes Ag § Ag
68.7
Ag §
97.2 96.9
Ag § Ag
99.7 99.8 94.4 99.8 100.0 97.7 100.0
0
7
4
0
0
0
0
2
3 1 1
0
0
1
2
0
1 2 1 0
0
0
0
0
0
0
0
0
0
1
0
Mi § Leucosporidium sp. Mi Rhodosporidium lusitaniae Sporodiobolus salmonicolor Sporobolomyces sp. Sporobolomyces sp. Ag Auriscalpium vulgare Eu Ag
0
1
1 1 1 1
0
0
2 0
0
0
0
1
0
0
1 1
3
1 0
0
1 C 1 C
0 82.0 99.5 89.0 99.5
0
1 1 1
1 1
99.5 99.6 99.3 65.8 81.0
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 1 1 1 1 2 1 1 1 1 1 0 0 0 0
0
0
2
1
0
0
1 1 1 1
1 2
1 1
0
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name
BF-OTU588 BF-OTU570 BF-OTU682 BF-OTU488 BF-OTU646, BF165 BF-OTU561, BF166 BF-OTU590 BF-OTU460 BF-OTU640 BF-OTU604 BF-OTU524 BF-OTU635 BF-OTU669 BF-OTU643 BF-OTU529 BF-OTU633 BF-OTU617 BF-OTU702 BF159 BF-OTU611 BF-OTU542 BF-OTU575 BF-OTU562 BF-OTU634 BF-OTU404 BF-OTU495 BF-OTU614 BF-OTU556 BF-OTU678 BF-OTU517 BF-OTU537 BF-OTU544
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession Cluster 35 - Tremellomycetes FR682331 Phlebiopsis gigantea AF087487 FR682313 Cryptococcus albidosimilis AF145331 Cluster 36 - Tremellomycetes FR682425 Filobasidium uniguttulatum DQ668348 Cluster 37 - Microbotryomycetes FR682231 Rhodotorula glutinis AM160642 FR682389, Rhodotorula mucilaginosa DQ386306 FR718484 Cluster 38 - Tremellomycetes FR682304, Cryptococcus adeliensis AF145328 FR718485 FR682333 Cryptococcus albidus AF444378 FR682203 Cryptococcus friedmannii AF145322 FR682383 Cryptococcus friedmannii AF145322 FR682347 Cryptococcus magnus AF190008 FR682267 Cryptococcus magnus AF190009 FR682378 Cryptococcus oeirensis AY188362 FR682412 Cryptococcus oeirensis AF444364 FR682386 Cryptococcus saitoi AF444372 FR682272 Cryptococcus wieringae AF444383 FR682376 Mrakia frigida AF144482 FR682360 Mrakia gelida AF144485 Mrakia sp. FR682445 AY038836 Cluster 41 - Tremellomycetes FR718483 Cryptococcus skinneri AF444305 FR682354 Cystofilobasidium capitatum AY052492 FR682285 Cystofilobas. infirmo-miniatum AF444400 FR682318 Cystofilobasidium macerans AF444317 FR682305 Trichosporon mucoides AF455482 Cluster 42 - Agaricomycetes FR682377 Exidia japonica AF291274 FR682147 Exidia pithya AY805609 Cluster 43 - Microbotryomycetes FR682238 Exobasidium pachysporum AB180352 FR682357 Exobasidium woronichinii AB180361 FR682299 Rhodotorula phylloplana AB038131 Cluster 44 - Basidiomycetes incertae sedis FR682421 Malassezia globosa AY743630 FR682260 Malassezia globosa AY387134 FR682280 Malassezia globosa AY743630 FR682287 Malassezia globosa AY387136
Sim %
Final annotation ** In1a 0
99.5 100.0
Phlebiopsis gigantea Cryptococcus sp. 0
99.8
Filobasidium uniguttulatum
99.8 100.0
Mi Rhodotorula mucilaginosa
0
7 99,7-100
Cryptococcus adeliensis
99.7 97.2 98.3 99.2 99.8 99.5 99.5 99.7 99.7 99.8 100.0 99.3
Cryptococcus albidus Cryptococcus sp. Cryptococcus sp. Cryptococcus magnus Cryptococcus magnus Cryptococcus oeirensis Cryptococcus oeirensis Cryptococcus saitoi Cryptococcus wieringae Mrakia sp. Mrakia sp. Mrakia sp.
96.1 99.8 100.0 99.8 99.8
Tr Cystofilobasidium capitatum C. infirmo-miniatum Cystofilobasidium macerans Trichosporon sp.
96.3 95.8 99.0
Exobasidium sp. Exobasidium sp. Rhodotorula sp.
98.1 98.7 99.3 99.6
Malassezia globosa Malassezia globosa Malassezia globosa Malassezia globosa
0
3
14 1
8 3
7 1
0 C
1 1 1 3
1 1 2
2
0
3
5
2
7 1 1
1
0
1 1 1 1 0
1
2
0 C
1 1 1
1 0
0
1 1
1 0
0
0
1 0
1
0
0
0
2 2 0
1 0
0
1
0
1 9
8
7 1
3
1 Ag Exidia pithya
0
2 2 1
1
93.9 100.0
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 0 0 1 1 0 0 1 0 1 1 1 0 0 0 0 3 0 0 0 3 2 0 0 0 6 1 0 0 2 6 1 C
1
1 16 1 2 1
5
2
1 1
9 1
2
10
1
1 2
0
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU536 BF-OTU535 BF-OTU420 BF-OTU401 BF-OTU554 BF-OTU522 BF-OTU429 BF-OTU432 BF-OTU567 BF-OTU539 BF-OTU696 BF-OTU683 BF-OTU594 BF-OTU514 BF-OTU658 BF-OTU461 BF-OTU584 BF-OTU533 BF-OTU549 BF-OTU470 BF-OTU580 BF-OTU444 BF-OTU563 BF-OTU452 BF-OTU655 BF-OTU560 BF-OTU434 BF-OTU603 BF-OTU581 BF-OTU680 BF-OTU660 BF-OTU673 BF-OTU578 BF-OTU674 BF-OTU579 BF-OTU525 BF-OTU523
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession FR682279 Malassezia restricta AJ437695 FR682278 Malassezia restricta AJ437695 FR682163 Malassezia restricta AJ437695 FR682144 Malassezia restricta AJ437695 FR682297 Malassezia restricta AY387145 FR682265 Malassezia restricta AJ437695 FR682172 Malassezia sympodialis AY743646 FR682175 Malassezia sympodialis AY743632 FR682310 Uncultured Malasseziales AY612334 Cluster 45 - Agaricomycetes FR682282 Tricholoma lascivum AY573542 Cluster 46 - Agaricomycetes FR682439 Calvatia turneri DQ112596 FR682426 Coprinopsis stercorea AY461828 FR682337 Cortinarius aprinus AY669663 FR682257 Cortinarius armillatus DQ114744 FR682401 Cortinarius pholideus AY669694 FR682204 Dermocybe semisanguinea DQ481909 FR682327 Handkea excipuliformis DQ112590 FR682276 Hebeloma mesophaeum AB211272 FR682292 Hypholoma capnoides AY805610 FR682213 Hypholoma sublateritium AY818349 FR682323 Lycoperdon pyriforme AY854075 Panaeolus sp. FR682187 DQ403253 Psathyrella cf. gracilis FR682306 AY228352 FR682195 Serpula lacrymans AJ536023 Cluster 47 - Unknown FR682398 Basidiomycete sp. AM084842 FR682303 Macrotyphula fistulosa AJ296348 FR682177 Uncultured basidiomycete AY969416 Cluster 48 - Agaricomycetes FR682346 Clitocybe nebularis DQ486691 FR682324 Clitocybe subditopoda EU669216 FR682423 Clitocybe subditopoda DQ202269 FR682403 Clitocybe subditopoda EU669216 FR682416 Collybia cirrhata AF274382 FR682321 Collybia tuberosa AF065124 FR682417 Coprinus comatus AF345823 FR682322 Panellus serotinus AY265847 FR682268 Tricholoma sejunctum AF241518 Cluster 49 - Agaricomycetes FR682266 Antrodia sitchensis AY966451
Sim %
Final annotation ** In1a
95.9 96.0 96.4 96.9 99.3 99.9 79.3 100.0 98.3 99.8 99.3 91.4 97.7 100.0 94.6 99.2 99.0 99.6 99.8 99.8 100.0 99.8 91.1 99.7
Malassezia sp. Malassezia sp. Malassezia sp. Malassezia sp. Malassezia restricta Malassezia restricta BaIs § Malassezia sympodialis Malassezia sp.
1 2 2 1 2 1
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 2 1 1 1
1
97.5 88.1 94.3
Ag Ag § Ag
99.5 98.9 99.8 99.2 95.5 93.6 91.4 99.3 99.4
Clitycybe sp. Clitocybe subditopoda Clitocybe subditopoda Clitocybe subditopoda Ag Ag § Ag Panellus serotinus Tricholoma sejunctum
99.5
Antrodia sp.
3 4
1
1 6
1
0
0
5
0
Tricholoma sp. Lycoperdon sp. Ag Cortinarius sp. Cortinarius armillatus Ag Dermocybe sp. Lycoperdon sp. Hebeloma sp. Hypholoma capnoides Hypholoma sublateritium Lycoperdon pyriforme Panaeolus sp. Ag Serpula lacrymans
1 4 4
1 1 4
1 0
0
0
0
0
0
1
2
2 1 1
0
3
0
1 1 1 1 1 1 1
1
2 1
1
0
0
0
1 1
0
0
3
5
1
0
1 1
0
0
3
0
1 1 1 1
0
2 2
1 0
1
1 1 2 1 1 1 1 6
9
0
1
1 7 6
0
1 3
2
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name BF-OTU657 BF-OTU437 BF-OTU626 BF-OTU475 BF-OTU664 BF-OTU482 BF-OTU701 BF-OTU435 BF-OTU441 BF-OTU402 BF-OTU518 BF-OTU625 BF-OTU670 BF-OTU442 BF-OTU665 BF-OTU466 BF-OTU569 BF-OTU720 BF-OTU490 BF-OTU509 BF-OTU502 BF-OTU645 BF-OTU492 BF-OTU439 BF-OTU553 BF-OTU661 BF-OTU428 BF-OTU424 BF-OTU399 BF-OTU538 BF-OTU547 BF-OTU566 BF-OTU501 BF036
rDNA Nearest Blast hit tree cluster*/ Organism Accession accession FR682400 Gloeophyllum abietinum AJ420947 FR682180 Gloeophyllum sepiarium AJ420946 FR682369 Phlebia radiata DQ056859 FR682218 Phlebiella vaga EU118660 FR682407 Pseudotomentella larsenii AF326981 FR682225 Resinicium furfuraceum DQ873648 FR682444 Steccherinum fimbriatum EU118668 Tomentellopsis sp. FR682178 AJ410782 FR682184 Trametes ochracea AB158314 Trametes sp. FR682145 AY840569 FR682261 Trametes versicolor AY309018 FR682368 Trechispora alnicola DQ411529 FR682413 Uncultured ectomycorrhiza AY641458 FR682185 Uncultured fungus DQ309195 Cluster 50 - Unknown Rhizoctonia sp. FR682408 AY927338 Cluster 51 - Unknown Bensingtonia sp. FR682209 DQ224375 FR682312 No full length match FR682462 No match Cluster 52 - Microbotryomycetes FR682233 Occultifur externus AF444643 FR682252 Rhodotorula lamellibrachiae AB263120 FR682245 Rhodotorula pinicola AF444292 FR682388 Rhodotorula slooffiae AF444589 Rhodotorula sp. FR682235 DQ317357 Cluster 53 - Pucciniomycetes FR682182 Melampsora caprearum AY444779 FR682296 Melampsoridium betulinum AF125177 FR682404 Melampsoridium hiratsukanum AY394707 FR682171 No full length match FR682167 Thekopsora areolata DQ087231 Cluster 54 - Agaricomycetes FR682142 Thelephora terrestris GQ205371 Cluster 55 - Saccharomycetes FR682281 Galactomyces geotrichum AJ279451 FR682290 Galactomyces geotrichum DQ148946 FR682309 Yarrowia lipolytica DQ683007 Cluster 56 Wallemiomycetes FR682244 Wallemia muriae AY302514 FR718458 Wallemia sebi AY302517
Sim %
Final annotation ** In1a
84.9 100.0 99.7 94.9 92.2 98.9 99.2 98.5 99.8 94.6 99.7 86.9 82.2 99.8
Ag Gloeophyllum sepiarium Phlebia radiata Ag Ag § Resinicium furfuraceum Steccherinum fimbriatum Tomentellopsis sp Trametes sp. Ag Ag Ag Ag Ag §
72.4
Ag
71.8
Ab UF UF
1 1
99.0 100.0 99.8 99.8
1 1 1 1 1 1 1 2 1 1 0 1 1
0
0
0
0
0
2
1
1 1 0
0
0
0
0
0
0
0
0
0
0
1
0
0
2 1
Cy Mi Rhodotorula pinicola Rhodotorula slooffiae Rhodotorula sp. Melampsora sp. Melampsoridium betulinum Melampsoridium hiratsukanum UF Thekopsora areolata
100.0
Thelephora terrestris
99.1 100.0 86.0
Galactomyces geotrichum Galactomyces geotrichum Sa Wallemia muriae Wallemia sebi
10
5 1 2 1
0
0
1 38 3 7 2 1 25 1 1 0
0 98.8 99.1
1
1
0 97.5 86.3 99.8 99.8 97.6
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 1
1 0
2
0
3
0
0
0
0
2 1
0
0
0
2 0
0
0
0
0
0
3 1 1 1 0
0
0
0
0
0
0
0
0
0
0
0
0
1 1
C
Table S1. Detection frequencies and annotation details of NucITS clones and cultivated isolates from house dust samples. Phylotype name
BF-OTU548 BF-OTU436 BF-OTU685
rDNA tree cluster*/ Organism accession Cluster 57 - Zygomycetes FR682291 Mucor hiemalis FR682179 Mucor racemosus FR682428 Zygomycete sp.
Nearest Blast hit Accession
Sim %
Final annotation ** In1a 1
AJ876490 AY213662 AY618254
99.8 99.8 99.3
Mucor hiemalis Mucor sp. ZyIs
Occurrence in dust and material samples *** Location 1 Location 2 Re1a In1b Re1b BM-1 In2a Re2a In2b Re2b BM-2 0 2 0 0 1 0 0 0 2
1
* rDNA tree cluster: position in the tree shown in Figure 3. The grey area includes the summed frequencies of the OTUs included in the cluster. ** Final annotation: the sequences were annotated by the formerly described method (Ciardo et al. 2007), after which the annotation was refined manually. Phylotypes were annotated to species, genus, class, phylum or as unknown. Abbreviations: Ag: Agaricomycetes; Ab: Agaricostilbomycetes; Art: Arthoniomycetes; AsIs: Ascomycetes incertae sedis; BaIs: Basidiomycetes incertae sedis; Cy: Cystobasidiomycetes; Do: Dothideomycetes; Eu: Eurotiomycetes; Lc: Lecanoromycetes; Le: Leotiomycetes; Mi: Microbotryomycetes; Pe: Pezizomycetes; Sa: Saccharomycetes; So: Sordariomycetes; Ta: Taphrinomycetes; Tr: Tremellomycetes; UA: unidentified ascomycete; UF: unidentifed fungus; ZyIs: Zygomycetes incertae sedis. § An unknown OTU which was either detected in two or more individual libraries or shares high similarity with published environmental sequence. *** The number of clones belonging to the phylotype in given library is shown. C: the phylotype was cultivated from the sample. The total number of clones in each rDNA tree cluster is shown on the grey field. For building material associated phylotypes, see Table S2 for additional information. Sample name abbreviations: In: index building; Re: reference building; 1: location 1; 2: location 2; a: before-renovation sample; b: after-renovation sample; BM: building material sample pool.
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