Table S1 Primers used in this study. Primer name Sequence (5 ... - PLOS

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This study. fhlA+2106/P2. CAGGCAGATCTGTCCGGCAATTTGCAGTTAAATCAATGCCcatatgaatatcctccttag. This study. glnG-50/P1.

Table S1

Primers used in this study.

Primer name

Sequence (5'→ 3')a

Source

TGGTAAACACTTGCCCCATA AGCGTCGACGTGATATTGCT CGCTATGCAGAAATGCTACG ACGTTCAGAAGCAGCGGTAT ATCGCCAAACGTTGGTATCT CAGGTGTTTTCATCCTGCAA CGTCGGTATTTTGATGACCA ATGACCGACACGGAAGAAAC CCTGACACCAGACGTCTTTC GCGTGTTCGTTCCTTTTCTG CACAAACCGGCAAAACCTAT ATGCATTACCCAGCAGTCCT ACCGTGGTACGCAATAAAGC AGTCTTTGCCGAGAGACGAA TAGATGCCAGAATGCAGCAC TGGCATAACCACATTGTTCG TATCGAAGAGGGCAACCTGG GTTCAATCGTCTGGCGAATC CGAGAGCAGGAAGTTCAA GTCCATCATCAGGCACAT ACTTCCAGCCTTCGTTCAGA TTCTGGAACGCTTCTTTCGT TCCCTCTCGATGATGCTACC TGTCGCTTGAACTGATTTCCT AGGCTGCGATTCTCATGTTT GAAGTTTGGCTTTCGCATTC

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CGGAATTCGGATTCACTCGCTTGCCGCC CGGGATCCAATAAATAATCTCCGCATGC ATTATCGATGGGTAGAAGTTTGCGACGTT AACACAAGCTTGTGTCTTCCTTATCGGTTGG

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GGAACAAGGTATCATGGTGGCACGCGCCACTGTTGCGAAGTACCGAGAGTCT AGACTCTCGGTACTTCGCAACAGTGGCGCGTGCCACCATGATACCTTGTTCC CTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTgtgtaggctggagctgcttc CAGGCAGATCTGTCCGGCAATTTGCAGTTAAATCAATGCCcatatgaatatcctccttag CATACCGAGTTCTCGGTTTACCTGCCTATCAGGAAATAAAgtgtaggctggagctgcttc CCGGGCAAGATCATACTGAACTTATCGGAACAGTAAAGCGcatatgaatatcctccttag TTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGgtgtaggctggagctgcttc TGCAAAAGCAGATTACAACACCTCGCGAACCGAGATCCCCcatatgaatatcctccttag ACCTCAATTTATTCAGCGTGTTCTAAAAAGATGTCTTGCTgtgtaggctggagctgcttc AGTTGTGATGATTTTGTGCCAGTGCCTGACGAATAGTTTCcatatgaatatcctccttag TAATCCGCAAATATGCGTTTCAGTTAACGTTTCAGGCAATgtgtaggctggagctgcttc CCTATGTAAACATCCCCGATGCGTAAGTTTATCGGTGATCcatatgaatatcctccttag

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qRT-PCR: gadE+309 gadE+419 gadX+319 gadX+413 gadW+445 gadW+536 adiC+987 adiC+1076 adiY+276 adiY+357 adiA+1463 adiA+1542 trmE+991 trmE+1083 ydeO+548 ydeO+631 rpoS+356 rpoS+466 ler+109 ler+214 tir+664 tir+869 cesT+296 cesT+445 espA+128 espA+310 Cloning: ler-430/EcoRI ler-1/BamHI rpoN-45/ClaI rpoN+1455/HindIII Mutation: rpoNR456-F rpoNR456-R fhlA-66/P1 fhlA+2106/P2 glnG-50/P1 glnG+1503/P2 hyfR-43/P1 hyfR+2004/P2 norR-107/P1 norR+1510/P2 prpR-117/P1 prpR+1676/P2

pspF-41/P1 pspF+1029/P2 rtcR-83/P1 rtcR+1564/P2 tyrR-94/P1 tyrR+1523/P2 yfhA+20/P1 yfhA+1333/P2 ygeV-77/P1 ygeV+1806/P2 zraR-14/P1 zraR+1312/P2 rpoS-56/P1 rpoS+1104/P2 rpoN-12/P1 rpoN+1430/P2 a

GCAACATGCCAGGATGAATTAGCTAATTACACTAACAAGTgtgtaggctggagctgcttc CACGCCGCATCCGGCAAGTTGTATTGCCCAACTTCGCTAAcatatgaatatcctccttag TTATATCTTTACGTCCGTAAACGGAGATTTCCCGCAAAGCgtgtaggctggagctgcttc ACGTCAGACCAAAACGCGCCAGGTATTTGCGTAGCCGATCcatatgaatatcctccttag TCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATgtgtaggctggagctgcttc TGGCTTAAGCCATATTCCCGCAACTTATTGGCAATCGCGGcatatgaatatcctccttag ATTTATTATTGGTCGATGACGATCCGGGATTGCTGAAACTgtgtaggctggagctgcttc ATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGcatatgaatatcctccttag GAGTTAATATGATCATGATCTGTGAACCATCAACGTCTTCgtgtaggctggagctgcttc CCTGAATTCAGGCCGGATTCACTGATGTTATGTGTTTAACcatatgaatatcctccttag TATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCgtgtaggctggagctgcttc TTGCCAACAGCGTTTTGCGCGTGATCCCTAACTGACGGGCcatatgaatatcctccttag GGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACgtgtaggctggagctgcttc TTGCCGGGTAGGACGCTGACGTGTCTTATCCAGGCGACAAcatatgaatatcctccttag ACGATTCTGAACATGAAGCAAGGTTTGCAACTCAGGCTTAgtgtaggctggagctgcttc ACGAGCTGTTTACGCTGGTTTGACGGCGGAATGGATAAAGcatatgaatatcctccttag

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Underlined nucleotides indicate restriction sites (cloning primers) or site-specific mutagenic regions (mutation primers).