TSC Source Data-2-final.pptx

39 downloads 0 Views 390KB Size Report
Source Data Fig 2. A. 130. 130. 250. 250. 95. 95. 72. 72. 55. 55. Tsc1. Tsc2. Hsp90. Hsp70. PP5. IP. Input. Co-IP. Input. Co-IP. Input. Co-IP. Input. Co-IP. Input.
Source Data Fig 2! A! A, cont.! IgG!

Tsc1! Ab!

IgG!

kDa!

Tsc1! Ab!

B! kDa!

55! 130! Input!

EV!

N!

M!

C!

kDa! 250!

130! IP! Tsc1!

Hsp90α-FLAG!

Co-IP! Tsc2!

Cdc37!

250! 55!

Co-IP!

Input!

Input! Co-IP!

250! Co-IP!

72! Co-IP!

Tsc1!

Raf-1! 72!

250! Input!

95!

130! Input! Input!

55! Co-IP!

Co-IP!

Hsp90α-FLAG!

Tsc2!

130!

55! 36! IP! 28! 17!

Akt!

Hsp90! 95!

Input! 55!

72!

Co-IP!

Input!

36!

Hsp70!

Co-IP! 72! Input! Cdk4! 36! Input! 55!

Co-IP! 95!

PP5!

Co-IP!

55!

GR! Input!

95!

GAPDH!

Input!

36! Total!

GAPDH!

36! Total!

Source Data Fig 2! C!

D!

Hsp90-FLAG!

EV!

β!

βΔTPR! α!

αΔTPR! kDa!

130! Co-IP!

B!

D!

kDa! 95!

Co-IP!

95!

Input!

72!

Co-IP!

72!

Input!

Hsp90!

Tsc1!

130!

Tsc1-FLAG! EV! WT! A!

Input!

Hsp70! 55!

Co-IP!

55!

Input!

HOP! 250! 130! 95! 72! Hsp90-FLAG!

Tsc1-FLAG! 95!

IP!

55!

IP!

36! 28! 17!

GAPDH!

36!

Total!

GAPDH!

36!

Total!

Source Data Fig 2! E!

I!

Hsp90α-FLAG! EV!

WT! N!

M!

C!

Tsc1-WT! C1! C2! C3! C4! C5!

kDa!

28!

Tsc1GFAPCKO ! K1! K2! K3! K4! K5! kDa!

Co-IP! 130! SE!

Tsc1-D-HA! 28! Input!

Tsc1!

LE! 130!

95! 72! Hsp90-FLAG!

55! 36!

IP!

28!

Ulk1!

17!

GAPDH!

Total! 36!

ErbB2!

Cdk4!

ER!

GR!

Hsp90!

130!

250!

36!

72!

95!

95!