TSC Source Data-3-final-correct.pptx

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Source Data Fig 3. Tsc1-FLAG. A. 250. 130. 72. 55. 55. 36. 250. 95. 95. 72. 130. 95. 36. ErbB2. Ulk1. Raf-1. p-c-Src (Y416) c-Src. Cdk4. Tsc2 p-GR (S211). GR.
Source Data Fig 3! A! B! Tsc1 MEF! +/+! ErbB2!

Ulk1!

Raf-1!

p-c-Src (Y416)!

–/–!

EV!

kDa! 250!

130!

72!

55!

Tsc1-HA!

2µg!

4µg! kDa!

B, cont.!

EV!

Tsc1-HA!

2µg!

4µg!

kDa!

250! ErbB2!

Ulk1!

130!

Raf-1!

72!

p-c-Src (Y416)!

Hsp90!

95!

GAPDH!

36!

C!

EV!

55!

Tsc1-! FLAG!

kDa! 250!

c-Src!

c-Src! 55!

Cdk4!

Tsc2!

36!

CFTR! 55!

Tsc2! Cdk4!

250!

130!

250!

36!

250!

Tsc1-FLAG!

130!

Tsc2! p-GR (S211)!

95! p-GR (S211)!

GR!

72!

Tsc1!

GR!

95!

FLCN!

72!

130!

Hsp90!

95!

GAPDH!

36!

Tsc1-HA!

Tsc1!

95!

GAPDH!

36!

95!

95!

FLCN!

Hsp90!

130!

130!

Source Data Fig 3! D!

Tsc1 Hsp90α–His6

+ –

– +

+! +!

E! kDa!

Tsc1 Tsc2

+ –

– +

+! +!

Co– ! 130! Pulldown!

Hsp90α–His6!

95! Pulldown!

+

+

+!

GB (µM)

0

1

10! kDa!

+

+

+!

+! +! +!

kDa!

130!

Input!

95! Co–IP!

250! IP!

Tsc2!

Hsp90α!

95! Input! Tsc1

+

+

Tsc2

+

+

GB (µM)

0

1

+! +!

10!

kDa!

130!

Tsc2!

Co-IP!

Tsc1!

130! Input!

95! Pulldown!

–! +! +!

130! Co–IP!

130! Input!

130! Co– ! Pulldown! Tsc1!

–! –! +!

Tsc1!

H!

Tsc1

Hsp90α–His6!

kDa!

130! Co-IP!

130!Input!

Hsp90α–His6

Tsc1! Hsp90α–His6! Tsc2!

Tsc1!

Tsc1!

G!

F!

130!

Tsc2!

Input!

250! IP!

250!

IP!

Source Data Fig 3! I!

Tsc1! Hsp90α–His6! Tsc2! GB (µM)

+! +! +! 0

+! +! +! 1

J!

+! +! +! 10! kDa!

130! Co–IP!

Tsc1! Hsp90α–His6!

+! +!

+! +!

GB (µM)

1

10!

Tsc1!

kDa!

130! Co–IP!

Tsc1! 130! Input!

95! Co–IP!

Hsp90α! 95!

Tsc2!

250!

Input!

IP!

Hsp90α!

95! IP !