TSC Source Data-5-final.pptx

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Source Data Fig 5. B. 130. 130. 55. 36. 55. 36. 95. IP. Input. Input. Co-IP. Co-IP. Tsc1. Aha1. Hsp90α-FLAG. EV. WT. Y313F Y313E. Hsp90α-FLAG. kDa. C. 130.
Source Data Fig 5! A!

C!

Tsc1 MEF! –/–! kDa! +/+!

55!

Aha1

Hsp90α-FLAG! EV!

WT!

V410E!

kDa! 130! Co–IP!

Co–IP! Tsc1!

!

55!

130! Input!

Input!

55! Hsp90!

95!

36!

IP!

Co–IP!

Aha1!

Tsc1!

130!

EV!

D!

Hsp90α-FLAG!

WT!

Input!

95!

IP!

36!

Total!

Hsp90α-FLAG!

B!

55!

Hsp90α-HA!

Y313F! Y313E! kDa!

Hsp90α-FLAG!

130! Co-IP! Tsc1! 130! Input!

–!

WT!

–!

WT! V410E!

WT! kDa!

130!

Co-IP!

Tsc1! 130! Input!

55! 36!

Co-IP!

55! 36!

Aha1! 55! 36!

Input!

Aha1! 55! 36!

Hsp90α-FLAG!

95!

Co-IP!

Input!

IP! Hsp90α-HA!

95! IP!

Hsp90α-FLAG!

95!

Source Data Fig 5! E!

H!

Tsc1-D! EV! -FLAG! kDa!

Hsp90α

Aha1–FLAG (ng)!



+



+

0

+

1

+

10

+

100

!

200 kDa! !

95! Co–IP! Hsp90! 95! Input! 55!

95! Co–Pulldown!

Hsp90α!

Co–IP!

Aha1! 55!

28!

Tsc1-D-FLAG!

F!

Tsc1-D-HA! Tsc1-D-FLAG!

–! –!

+! +!

Input!

Tsc1–D–His6 !

28!

IP!

I! kDa! 28!

Hsp90α

Tsc1–D–His6 (ng)! Co-IP!

Hsp90α!



+

+

+

+



0

0.1

1

10

! ! kDa!

95!

Co–IP!

Tsc1-D-HA! 28! Input!

Tsc1-D-FLAG!

GAPDH!

Pulldown!

28!

IP!

36! Total!

Aha1–FLAG!

55! 36!

IP!