BAKTERI ASAM LAKTAT DALAM MEMPERBAIKI KERUSAKAN. JARINGAN
HATI MENCIT (Mus musculus L.) YANG DIPAPAR TIMBAL. ABSTRAK. Paparan
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EFEKTIFITAS PEMBERIAN SOYGHURT YANG MENGANDUNG BAKTERI ASAM LAKTAT DALAM MEMPERBAIKI KERUSAKAN JARINGAN HATI MENCIT (Mus musculus L.) YANG DIPAPAR TIMBAL
ABSTRAK Paparan timbal dapat mengakibatkan kerusakan sel-sel hepatosit yang berakibat meningkatnya kadar enzim Serum Glutamat Oksaloasetat Transaminase (SGOT) dan Serum Glutamat Piruvat Transaminase (SGPT) dalam darah yang sering menjadi indikator adanya kerusakan hati. Tujuan penelitian ini ialah untuk pembuatan soyghurt dengan menggunakan starter soyghurt yang mengandung bakteri asam laktat Lactobacillus bulgaricus dan Streptococcus thermophilus, dan melihat manfaatnya dalam memperbaiki kadar SGOT dan SGPT yang dihasilkan oleh jaringan hati serta gambaran makroskopis dan histopatologi hati mencit (Mus musculus L.) jantan yang diberi timbal. Perlakuan waktu fermentasi pada pembuatan soyghurt ialah 4, 6, dan 8 jam pada suhu inkubasi 40 oC. Analisa jumlah bakteri asam laktat berdasarkan Standard Plate Count. Identifikasi jenis bakteri dilakukan dengan pengamatan karakteristik sifat morfologi dan sifat fisiologis bakteri. Sifat morfologi dilakukan uji pewarnaan Gram dan motilitas bakteri, sedangkan sifat fisiologisnya dilakukan uji katalase, indol, H 2 S, pembentukan gas, fermentasi glukosa, penggunaan sitrat, reduksi nitrat, hidrolisa pati, ketahanan suhu, dan fermentasi karbohidrat. Hasil penelitian menunjukkan bahwa waktu fermentasi yang baik pada pembuatan soyghurt adalah 8 jam dengan jumlah koloni sebesar 1,71 x 109 cfu/ml. Pemberian timbal meningkatkan kadar SGOT dengan nilai (544,00±30,26 IU/ml), kadar SGPT (116,43±11,13 IU/ml) dan kerusakan sel hati dengan nilai (28,57±8,13). Sebaliknya pemberian soyghurt dapat menurunkan kadar SGOT (456,29±26,87 IU/ml), kadar SGPT (85,86±7,06 IU/ml) dan perbaikan sel-sel hati (19,05±6,30); p < 0.05. Hasil menunjukkan bahwa soyghurt yang mengandung bakteri asam laktat efektif dalam memperbaiki kerusakan jaringan hati mencit yang terpapar timbal. Katakunci:
bakteri asam laktat, fermentasi soyghurt, makroskopis dan histopatologi hati, SGOT, SGPT
THE EFFECTIVENESS OF SOYGURT CONTAINING LACTIC ACID BACTERIA IN REPAIRING LIVER TISSUE DAMAGE OF LEAD EXPOSED MICE (Mus musculus L.)
ABSTRACT
The influence of metalloid lead again the liver cells damage, which may consequently increase of Glutamat Oksaloasetat Transaminase Serum (SGOT) and Glutamat Piruvat Transaminase Serum (SGPT), in the blood plasma and therefore both of them frequently used as indicators of liver cells damage. The aim of this study was to investigate the production of soygurt which lactic acid bacteria (Lactobacillus bulgaricus and Streptococcus thermophilus) as well as its benefit in improving the levels of SGOT and SGPT, also the macroscopic and histopahologic of mice (Mus musculus L.) to be exposed by lead. During the fermentation, the soygurt was incubated at 40 0C for 4, 6, and 8 hours. Total colony count of the lactic acid bacteria was carried out using Standard Plate Count, whilst the identification of the type of the lactic acid bacteria was performed the morphological and physiological characteristics of the bacteria. The morphological characteristic was tested using Gram staining and motility of the bacteria, while the physiological characteristic was tested by the catalase, indol, H 2 S, the gas formation, the glucose fermentation, the utilization of citrate, reduction of nitrate, hydrolysis of starch, resistance to temperature and carbohydrate fermentation. The result showed that the optimal fermentation time of soygurt production was 8 hours and the total colony was 1,71 x 109 cfu/ml. Exposure to lead increased the levels of mean ± S.D of the SGOT (554.00 ± 30.26 IU/ml), SGPT (116.43 ± 11.13 IU/ml) and hepatocyte cell damage (28.57 ± 8.13). In contrary, the administration of soygurt reduced the levels of SGOT (456. 29 ± 26.87 IU/ml), SGPT (85.86 ± 7.06 IU/ml) and improvement of hepatocytes (19.05 ± 6.03); p < 0.05. The result demonstrated that soygurt containing lactic acid bacteria was effective in repairing liver tissue damage in lead-exposed mice. Keywords:
lactic acid bacteria, soygurt fermentation, macroscopy and histopathology of the liver, SGOT, SGPT
