Additional file 1 - Springer Static Content Server
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timestampOn(verbose). Command used to define the Affymetrix chip type: chiptype
Additional file 1 Supplementary Methods R code for running the FIRMA algorithm Preprocessing and alternative splicing detection on CEL-files storing data from the Affymetrix GeneChip Human Exon 1.0 ST Array were done using the FIRMA algorithm [29] implemented with an R code in the aroma.affymetrix Bioconductor package [42]. R codes were retrieved from the aroma.affymetrix web page [43]. A custom made chip definition file for the core set of exons (HuEx-1_0-st-v2,coreR3,A20071112,EP.cdf) was downloaded from aroma.affymetrix [31].
The folder structures for reading input data were as follows: CEL-files: aroma.affymetrix\rawData\a\HuEx-1_0-st-v2; a = subfolder name CDF-files: aroma.affymetrix\annotationData\chipTypes\HuEx-1_0-st-v2
Commands used to set up the working directory and load the Bioconductor library: setwd("aroma.affymetrix") library(aroma.affymetrix) verbose
