PDIA3 protein disulfide isomerase family A, member 3. Cytoplasm peptidase. P18669. PGAM1 phosphoglycerate mutase 1 (brain). Cytoplasm phosphatase.
Additional File 1 Supplementary Tables Supplementary Table S1 Proteins identified from a pituitary adenoma tissue with two-dimensional gel electrophoresis and mass spectrometry ID P04217 P32391
Notes Molecules A1BG ACTR3
P30566 P30566 P15121
D D
O43488 P02768 P02768 P02768 P02768 P04083 P07355 P09525 P08758 P08758 P02743 P02647 P02647 P06576
O75947
ADSL ADSL AKR1B1 AKR7A2
D D D D
D D D D
ALB ALB ALB ALB ANXA1 ANXA2 ANXA4 ANXA5 ANXA5 APCS APOA1 APOA1 ATP5B
ATP5H (includes EG:10476)
Description Location alpha-1-B glycoprotein Extracellular Space ARP3 actin-related protein 3 homolog Plasma Membrane (yeast) adenylosuccinate lyase Cytoplasm adenylosuccinate lyase Cytoplasm aldo-keto reductase family 1, member B1 Cytoplasm (aldose reductase) aldo-keto reductase family 7, member A2 Cytoplasm (aflatoxin aldehyde reductase) albumin Extracellular Space albumin Extracellular Space albumin Extracellular Space albumin Extracellular Space annexin A1 Plasma Membrane annexin A2 Plasma Membrane annexin A4 Plasma Membrane annexin A5 Plasma Membrane annexin A5 Plasma Membrane amyloid P component, serum Extracellular Space apolipoprotein A-I Extracellular Space Extracellular Space apolipoprotein A-I ATP synthase, H+ transporting, Cytoplasm mitochondrial F1 complex, beta polypeptide ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Cytoplasm
Function other other enzyme enzyme enzyme enzyme transporter transporter transporter transporter other other other other other other transporter transporter transporter
transporter
Drugs
sorbinil
P30043
BLVRB
P01024 P00915
C3 CA1 (includes EG:759)
P27797 P04632 P04040 P35520 P51946 P49368
CALR CAPNS1 CAT CBS CCNH CCT3
P29373 P32321 O00559
CRABP2 DCTD EBAG9
P30084
ECHS1
P10126
EEF1A1
P26641
EEF1G
P06733 P09104 P30040
ENO1 ENO2 ERP29
biliverdin reductase B (flavin reductase (NADPH)) complement component 3 carbonic anhydrase I
calreticulin calpain, small subunit 1 catalase cystathionine-beta-synthase cyclin H chaperonin containing TCP1, subunit 3 (gamma) cellular retinoic acid binding protein 2 dCMP deaminase estrogen receptor binding site associated, antigen, 9 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial eukaryotic translation elongation factor 1 alpha 1 eukaryotic translation elongation factor 1 gamma enolase 1, (alpha) enolase 2 (gamma, neuronal) endoplasmic reticulum protein 29
Cytoplasm
enzyme
Extracellular Space Cytoplasm
peptidase enzyme
Cytoplasm Cytoplasm Cytoplasm Cytoplasm Nucleus Cytoplasm
transcription regulator peptidase enzyme enzyme transcription regulator other
Cytoplasm Unknown Cytoplasm
transporter enzyme other
Cytoplasm
enzyme
Cytoplasm
translation regulator
Cytoplasm
translation regulator
Cytoplasm Cytoplasm Cytoplasm
transcription regulator enzyme transporter
methazolamide, hydrochlorothiazide, acetazolamide, trichloromethiazide, dorzolamide, chlorothiazide, dorzolamide/timolol, brinzolamide, chlorthalidone, benzthiazide, sulfacetamide, topiramate
P00742
Q9NRD0 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 Q14192 Q13451 P02794 P02792 P06241 P50395 P01241
D D D D D D D D D D D D D D D D D D
F10
coagulation factor X
Extracellular Space
peptidase
FBXO8 FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FHL2 FKBP5 FTH1 FTL FYN
F-box protein 8 fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain four and a half LIM domains 2 FK506 binding protein 5 ferritin, heavy polypeptide 1 ferritin, light polypeptide FYN oncogene related to SRC, FGR, YES
Unknown Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Nucleus Nucleus Cytoplasm Cytoplasm Plasma Membrane
other other other other other other other other other other other other other other other other other other other other enzyme enzyme other kinase
GDI2 GH1
GDP dissociation inhibitor 2 growth hormone 1
Cytoplasm Extracellular Space
other cytokine
antihemophilic factor, dalteparin, heparin, coagulation Factor VIIa, enoxaparin, coagulation factor IX, rivaroxaban, deligoparin, idraparinux, tifacogin, RPR 120844, RPR 208566, DPC 423, fondaparinux thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin
dasatinib
P09471
D
GNAO1
P30443
D
HLA-A
guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O guanine nucleotide binding protein (G protein), beta polypeptide 2 guanine nucleotide binding protein (G protein), beta polypeptide 3 gelsolin (amyloidosis, Finnish type) gelsolin (amyloidosis, Finnish type) glutathione S-transferase M2 (muscle) glutathione S-transferase omega 1 glutathione S-transferase omega 1 glutathione S-transferase pi 1 hydroxyacyl-Coenzyme A dehydrogenase/3 ketoacyl-Coenzyme A thiolase/enoylCoenzyme A hydratase (trifunctional protein), beta subunit major histocompatibility complex, class I, A
P29777
D
GNAO1
P30455
D
HLA-A
major histocompatibility complex, class I, A Plasma Membrane
HNRNPC
heterogeneous nuclear ribonucleoprotein C (C1/C2) hemopexin hemopexin hydroxysteroid (17-beta) dehydrogenase 10 heat shock transcription factor 1 heat shock transcription factor 1 heat shock 70kDa protein 5 (glucoseregulated protein, 78kDa) heat shock 70kDa protein 8 heat shock 70kDa protein 8 heat shock 27kDa protein 1
P11016
GNB2
P16520
GNB3
P06396 P06396 P28161 P78417 P78417 P09211 P55084
D D D D
P07910
GSN GSN GSTM2 GSTO1 GSTO1 GSTP1 HADHB
P02790 P02790 Q99714
D D
HPX HPX HSD17B10
Q00613 Q00613 P11021
D D
HSF1 HSF1 HSPA5
P11142 P11142 P04792
D D
HSPA8 HSPA8 HSPB1
Plasma Membrane
enzyme
Plasma Membrane
enzyme
Plasma Membrane
enzyme
Plasma Membrane
enzyme
Extracellular Space Extracellular Space Cytoplasm Cytoplasm Cytoplasm Cytoplasm Cytoplasm
other other enzyme enzyme enzyme enzyme enzyme
Plasma Membrane
Nucleus
transmembrane receptor transmembrane receptor other
Extracellular Space Extracellular Space Cytoplasm
transporter transporter enzyme
Nucleus Nucleus Cytoplasm
transcription regulator transcription regulator other
Cytoplasm Cytoplasm Cytoplasm
enzyme enzyme other
O75874
D
IDH1
O75874
D
IDH1
P50213
IDH3A
Q9P0V2
IMMT
Q9UBX7 P08727 P35527 P07195 O75198 P41279
D
P41279
D
P40926 P16475
D
P24572
D
P24844
P54920 O75489
P22392 O75665 O14753 O14753
D D
isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 3 (NAD+) alpha
inner membrane protein, mitochondrial (mitofilin) KLK11 kallikrein-related peptidase 11 KRT19 keratin 19 KRT9 keratin 9 (epidermolytic palmoplantar keratoderma) LDHB lactate dehydrogenase B LOC390688 CDC37-like MAP3K8 mitogen-activated protein kinase kinase kinase 8 MAP3K8 mitogen-activated protein kinase kinase kinase 8 MDH2 malate dehydrogenase 2, NAD (mitochondrial) MYL6 myosin, light chain 6, alkali, smooth muscle and non-muscle MYL6 myosin, light chain 6, alkali, smooth muscle and non-muscle MYL9 myosin, light chain 9, regulatory (includes EG:10398) NAPA N-ethylmaleimide-sensitive factor attachment protein, alpha NDUFS3 NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase) NME2 non-metastatic cells 2, protein (NM23B) expressed in OFD1 oral-facial-digital syndrome 1 OVOL1 ovo-like 1(Drosophila) OVOL1 ovo-like 1(Drosophila)
Cytoplasm
enzyme
Cytoplasm
enzyme
Cytoplasm
enzyme
Cytoplasm
other
Extracellular Space Cytoplasm Cytoplasm
peptidase other other
Cytoplasm Unknown Cytoplasm
enzyme other kinase
Cytoplasm
kinase
Cytoplasm
enzyme
Cytoplasm
other
Cytoplasm
other
Cytoplasm
other
Cytoplasm
transporter
Cytoplasm
enzyme
Nucleus
kinase
Plasma Membrane Nucleus Nucleus
other transcription regulator transcription regulator
P07237
P4HB
Q29459
Q15365
P30101 P18669 O95336 P35232
procollagen-proline, 2-oxoglutarate 4dioxygenase (proline 4-hydroxylase), beta polypeptide PAFAH1B2 platelet-activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa
Cytoplasm
enzyme
Cytoplasm
enzyme
PCBP1 (includes EG:5093) PDIA3
poly(rC) binding protein 1
Nucleus
translation regulator
protein disulfide isomerase family A, member 3 phosphoglycerate mutase 1 (brain) 6-phosphogluconolactonase prohibitin
Cytoplasm
peptidase
Cytoplasm Cytoplasm Nucleus
phosphatase enzyme transcription regulator
phosphatidylinositol transfer protein, alpha
Cytoplasm
transporter
Unknown
ion channel
Cytoplasm
enzyme
Q00169
PGAM1 PGLS PHB (includes EG:5245) PITPNA
Q9NTG1
PKDREJ
P05092
D
PPIA
polycystic kidney disease (polycystin) and REJ homolog (sperm receptor for egg jelly homolog, sea urchin) peptidylprolyl isomerase A (cyclophilin A)
P05092
D
PPIA
peptidylprolyl isomerase A (cyclophilin A)
Cytoplasm
enzyme
P32119 P32119 P30041 O43422
D D
PRDX2 PRDX2 PRDX6 PRKRIR
peroxiredoxin 2 peroxiredoxin 2 peroxiredoxin 6 protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) proteasome (prosome, macropain) 26S subunit, ATPase, 1 proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
Cytoplasm Cytoplasm Cytoplasm Nucleus
enzyme enzyme enzyme other
Nucleus
peptidase
Cytoplasm
other
Cytoplasm
peptidase
Q03527
PSMC1
Q06323
PSME1
Q9UL46
PSME2
N-methyl-4-Ilecyclosporin N-methyl-4-Ilecyclosporin
P09455 P05121
RBP1 SERPINE1
Q13435 P00441
SF3B2 SOD1
P30626 Q9P2R7
D
SRI SUCLA2
Q9P2R7
D
SUCLA2
D D
TF TPI1 TPM3 TPM3 TPM4 UCHL1
P02787 P00938 P06753 P06753 P07226 P09936 Q99536
VAT1
P45880 P12956
VDAC2 XRCC6
P31946
D
YWHAB
P31946
D
YWHAB
P42655
YWHAE
P27348
YWHAQ (includes EG:10971)
retinol binding protein 1, cellular serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 splicing factor 3b, subunit 2, 145kDa superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) sorcin succinate-CoA ligase, ADP-forming, beta subunit succinate-CoA ligase, ADP-forming, beta subunit transferrin triosephosphate isomerase 1 tropomyosin 3 tropomyosin 3 tropomyosin 4 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) vesicle amine transport protein 1 homolog (T. californica) voltage-dependent anion channel 2 X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa) tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, beta polypeptide tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, beta polypeptide tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, epsilon polypeptide tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, theta polypeptide
Extracellular Space Extracellular Space
transporter other
Nucleus Cytoplasm
other enzyme
Cytoplasm Cytoplasm
transporter enzyme
Cytoplasm
enzyme
Extracellular Space Cytoplasm Cytoplasm Cytoplasm Cytoplasm Cytoplasm
transporter enzyme other other other peptidase
Plasma Membrane
transporter
Cytoplasm Nucleus
ion channel enzyme
Cytoplasm
other
Cytoplasm
other
Cytoplasm
other
Cytoplasm
other
drotrecogin alfa
P08107 U gi2460318 U gi14249382 U P02023 U gi12805429 U P38607 U
Heat shock 70 kDa protein 1 RNA-binding protein regulatory subunit Hypothetical protein MGC15429 Hemoglobin beta chain Similar to DJ-1 protein Vacuolar ATP synthase catalytic subunit A, osteoclast isoform P10990 U Actin 15A Note: D = duplicate. U = unmapped. All IDs = 154. Mapped IDs = 147. Unmapped IDs = 7. Network eligible IDs = 106.
Supplementary Table S2 Differentially Expressed Proteins Identified From Human Pituitary Adenoma tissues with 2DGE-based Comparative Proteomics ID
Notes
Molecules
P49753 P15121
ACOT2 AKR1B1
FoldChange 5.8 14.6
P02647 P06576
APOA1 ATP5B
-3.2 5.0
P47756
D
CAPZB
6.5
CAPZB
D
CAPZB
6.5
P13987
CD59
-9.5
P12110 P14854
COL6A2 COX6B1
-14.7 9.3
Q14894 CSN1S1
-35.3 -17.8
P07108
CRYM CSN1S1 (includes EG:1446) DBI
Q9UHL4 Q14259
DPP7 ERH
-9.3 5.0
P30040
ERP29
4.6
4.3
Description
Location
Function
Drugs
acyl-CoA thioesterase 2 aldo-keto reductase family 1, member B1 (aldose reductase) apolipoprotein A-I ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide capping protein (actin filament) muscle Zline, beta capping protein (actin filament) muscle Zline, beta CD59 molecule, complement regulatory protein collagen, type VI, alpha 2 cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous) crystallin, mu casein alpha s1
Cytoplasm Cytoplasm
enzyme enzyme
orbinil
Extracellular Space Cytoplasm
transporter transporter
Cytoplasm
other
Cytoplasm
other
Plasma Membrane
other
Extracellular Space Cytoplasm
other enzyme
Cytoplasm Extracellular Space
enzyme other
Cytoplasm
other
Cytoplasm Nucleus
peptidase other
Cytoplasm
transporter
diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein) dipeptidyl-peptidase 7 enhancer of rudimentary homolog (Drosophila) endoplasmic reticulum protein 29
collagenase
P00742
F10
-83.0
P06241
FYN
3.9
GDI1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1
9.4 -180.6 -13.3 -13.3 -20.2 -14.1 -128.3 -14.8 -48.1 -17.3 -16.9 -88.8 -27.8 -51.2 -32.1 -478.6
P31150 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241
D D D D D D D D D D D D D D D
coagulation factor X
Extracellular Space
peptidase
antihemophili c factor, dalteparin, heparin, coagulation Factor VIIa, enoxaparin, coagulation factor IX, rivaroxaban, deligoparin, idraparinux, tifacogin, RPR 120844, RPR 208566, DPC 423, fondaparinux
FYN oncogene related to SRC, FGR, YES
Plasma Membrane
kinase
dasatinib
GDP dissociation inhibitor 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1
Cytoplasm Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space
other cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine
P01241 P01241 P01241 P01242 Q04760 Q04760 P09471
P29777
D D D D D D
GH1 GH1 GH1 GH2 GLO1 GLO1 GNAO1
-13.4 -161.0 -37.0 -14.1 10.2 8.3 9.5
GPX4
-26.1 4.2 -3.1
-99.4
hemoglobin, beta
Cytoplasm
transporter
P02081 P14625
GSTM2 HBB (includes EG:3043) HBB (includes EG:3043) HBD HSP90B1
growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 2 glyoxalase I glyoxalase I guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O glutathione peroxidase 4 (phospholipid hydroperoxidase) glutathione S-transferase M2 (muscle) hemoglobin, beta
D
GNAO1
9.5
-3.1 -11.0
hemoglobin, delta heat shock protein 90kDa beta (Grp94), member 1
Cytoplasm Cytoplasm
transporter other
P04792 Q9UJY1 O75874
HSPB1 HSPB8 IDH1
-5.1 -3.7 8.7
Cytoplasm Cytoplasm Cytoplasm
other kinase enzyme
P24592
IGFBP6
-20.9
heat shock 27kDa protein 1 heat shock 22kDa protein 8 isocitrate dehydrogenase 1 (NADP+), soluble insulin-like growth factor binding protein 6
Extracellular Space
other
IGLC1
IGLC1
-32.1
immunoglobulin lambda constant 1 (Mcg marker)
Cytoplasm
other
P36969 P28161 HBB
D
HBB
D
Extracellular Space Extracellular Space Extracellular Space Extracellular Space Cytoplasm Cytoplasm Plasma Membrane
cytokine cytokine cytokine other enzyme enzyme enzyme
Plasma Membrane
enzyme
Cytoplasm
enzyme
Cytoplasm Cytoplasm
enzyme transporter
17dimethylamin oethylamino17demethoxygel danamycin, IPI-504
IGLC3
IGLC3
-32.1
P08779
KRT16
-28.8
Q86U44 Q99542 P12524
METTL3 MMP19 MYCL1
-8.3 3.1 -17.8
O00217
NDUFS8
5.2
P20774 Q08752
OGN PPID
-38.0 5.1
Q00007
PPP2R2A
-8.2
PRL PRL PRL PRL PRL PRL SCGN
-99.9 -9.7 -26.2 -20.1 -36.7 -33.6 -6.6
Q9UI15 P21980
TAGLN3 TGM2
5.6 -17.1
Q8IWU9 O14530 P08670 P27348
TPH2 TXNDC9 VIM YWHAQ (includes EG:10971) ZNF266
10.6 -11.4 5.5 -43.5
P01236 P01236 P01236 P01236 P01236 P01236 O76038
Q14584
D D D D D D
7.3
immunoglobulin lambda constant 3 (KernOz+ marker) keratin 16 (focal non-epidermolytic palmoplantar keratoderma) methyltransferase like 3 matrix metallopeptidase 19 v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian) NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) osteoglycin peptidylprolyl isomerase D (cyclophilin D) protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha isoform prolactin prolactin prolactin prolactin prolactin prolactin secretagogin, EF-hand calcium binding protein transgelin 3 transglutaminase 2 (C polypeptide, proteinglutamine-gamma-glutamyltransferase) tryptophan hydroxylase 2 thioredoxin domain containing 9 vimentin tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, theta polypeptide (14-3-3 protein tau) zinc finger protein 266
Unknown
other
Cytoplasm
other
Nucleus Extracellular Space Nucleus
enzyme peptidase transcription regulator
Cytoplasm
enzyme
Extracellular Space Cytoplasm
growth factor enzyme
Cytoplasm
phosphatase
Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Cytoplasm
cytokine cytokine cytokine cytokine cytokine cytokine other
Unknown Cytoplasm
other enzyme
Unknown Unknown Cytoplasm Cytoplasm
enzyme other other other
Nucleus
other
IL15-S21AA U P01620 IGLC2 P01621 P04433 HBB2 gi 1066765 HBB2 P01935 P01968 HBB2
U U U U U U U U U U
P40933-2
P01842
P18988 P18988
P18988
-2.7 -32.1 -32.1 -32.1 -32.1 -3.1 -176.7 -99.4 -99.4 -20.2 -20.2
Splice isoform IL15-S21AA of interleukin15 Ig kappa chain V-III region SIE Ig lambda chain C regions Ig Kappa chain V-III region NG9 Ig kappa chain V-III region VG Hemoglobin beta-2 chain (PANLE) Hemoglobin beta unit variant Hemoglobin beta-2 chain (PANLE) Hemoglobin alpha-3 chain (PANTR) Hemoglobin alpha-2 chain (BOSMU) Hemoglobin beta-2 chain (PANLE)
Note: D = duplicate. U = unmapped. All IDs = 86. Mapped IDs = 75. Unmapped IDs = 11. Network eligible IDs = 47.
Supplementary Table S3 Nitroproteins, and Proteins That Interact With Nitroproteins, Identified From Human Pituitary Adenoma Tissue With Nitrotyrosine Affinity Enrichment and Tandem Mass Spectrometry ID Q13017 P59901
Notes nY; NIE
Molecules ARHGAP5 LILRA4
O94892 P31321
nY; NIE nY
ZNF432 PRKAR1B
Description Rho GTPase activating protein 5 leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4 zinc finger protein 432 protein kinase, cAMP-dependent, regulatory, type I, beta
Location Cytoplasm Plasma Membrane
Function enzyme other
Nucleus Cytoplasm
other kinase
O95470 Q15027 P25787
nY nY nY
SGPL1 CENTB1 PSMA2
sphingosine-1-phosphate lyase 1 Cytoplasm centaurin, beta 1 Nucleus proteasome (prosome, macropain) subunit, alpha type, 2 Cytoplasm
Drugs
enzyme other peptidase
Q9UHA7 nY IL1F6 interleukin 1 family, member 6 (epsilon) Extracellular Space cytokine Q8IUC4 nY RHPN2 rhophilin, Rho GTPase binding protein 2 Cytoplasm other O43187 Y IRAK2 interleukin-1 receptor-associated kinase 2 Plasma Membrane kinase Q9C0E4 Y GRIP2 glutamate receptor interacting protein 2 Plasma Membrane other P62988 Y UBB ubiquitin B Cytoplasm other Note: nY = nitrotyrosine. Y = tyrosine. All IDs = 12. Mapped IDs = 12. Unmapped IDs = 0. Network eligible IDs = 10. NIE = Network illegibile.
Supplementary Table S4 Nitroproteins Identified From Human Pituitary Post-mortem Control Tissue With 2DGE-based Nitrotyrosine Western Blot and Tandem Mass Spectrometry ID P03996 P04270 P12718 P24071
Notes Molecules nY ACTA2 (includes EG:59) nY ACTC1 nY ACTG2 (includes EG:72) nY FCAR
Description actin, alpha 2, smooth muscle, aorta
Location Cytoplasm
Function other
actin, alpha, cardiac muscle 1 actin, gamma 2, smooth muscle, enteric
Cytoplasm Cytoplasm
other other
Fc fragment of IgA, receptor for
Plasma Membrane Q8IWL3 nY HSCB HscB iron-sulfur cluster co-chaperone homolog Cytoplasm Q6TCH7 nY PAQR3 progestin and adipoQ receptor family member III Cytoplasm Q13237 nY PRKG2 protein kinase, cGMP-dependent, type II Cytoplasm P25787 nY PSMA2 proteasome (prosome, macropain) subunit, alpha type, 2 Cytoplasm O60641 nY SNAP91 synaptosomal-associated protein, 91kDa homolog Plasma Membrane P52823 nY STC1 stanniocalcin 1 Extracellular Space Note: nY = nitrotyrosine. All IDs = 10. Mapped IDs = 10. Unmapped IDs = 0. Network eligible IDs = 9.
other other other kinase peptidase other kinase
Drugs
Supplementary Table S5 Clinical and Pathological Characteristics of Pituitary Adenomas and Controls Used in Comparative Proteomics Study Patient
Sex, Age
Clinical features/Tumor size
Immunohistochemistry
Reference
T164
M, 35
Nonfunctional, visual loss, 3 x 3.5 x 4 cm. Particial hypopituitarism
Neg.
[2]
T219
M, 68
Nonfunctional, 1.9 x 2.3 x 2.2 cm, invasion of the right cavernous sinus
Neg.
[2]
T165
M, 56
Nonfunctional, 3.2 cm in diameter craniocaudal
LH 2+
[2]
T208
F, 47
Nonfunctional, 2 x 2 x 2 cm
LH 1-2+
[2]
T237
F, 40
Nonfunctional, right cavernous sinus extension
LH 2+
[2]
T57
F, 59
Nonfunctional, 2 x 3 cm
FSH 1+
[2]
T77
M, 67
Nonfunctional, 2 x 2.2 x 2.4 cm, questionable cavernous sinus
FSH 2+
[2]
T89
M, 62
Nonfunctional, 2 x 2.3 x 2.3 cm
FSH 2+
[2]
T65
F, 54
Nonfunctional, 4 x 4 x 4 cm, cavernous sinus invasion
FSH 1+, LH 2+
[2]
T138
M, 60
Nonfunctional, 2.9 x 3.1 x 3.5 cm
FSH 2+, LH 2+
[2]
T185
M, 66
Nonfunctional, 2.8 x 2 x 2.4 cm, bilateral cavernous sinus invasion
FSH 2-3+, LH 2-3+
[2]
T87
M, 48
Prolactinoma with calcification
PRL+
[3]
T131
F, 52
Prolactinoma, serum 359 ng/ml, 2.5 x 3.5 x 2.8 cm
PRL 3+
[3]
T192
M, 41
Prolactinoma, serum PRL 1176 ng/ml, 3 x 2.5 x 2.0 cm
PRL 3+
[3]
T273
M, 36
Prolactinoma, serum PRL 1918 ng/ml, 2.0 x 2.1 x 2.5 cm
PRL 3+
[3]
C2
M, 27
Normal
ND
[2, 3]
C3
F, 40
Normal
ND
[2, 3]
C4
M, 45
Normal
ND
[2, 3]
C5
M, 36
Normal
ND
[2, 3]
C7
F, 34
Normal
ND
[2, 3]
C8
F
Normal
ND
[2, 3]
C9
M, 55
Normal
ND
[2, 3]
C10
F, 47
Normal
ND
[2, 3]
Adenomas were graded blindly by a neuropathologist from 0 to 4 for intensity of immunohistochemistry staining for each peptide hormone. Neg. = immunohistochemical stains for ACTH, LH, FSH, PRL, GH, and TSH were negative. LH+ = Nonfunctional (NF) adenoma that expressed leuteinizing hormone, or lutropin; FSH+ = NF that expressed follicle-stimulating hormone, or follitropin; FSH+, LH+ = NF that expressed both follicule-stimulating hormone and leuteinizing hormone; PRL+ = Prolactinoma that expressed prolactin. ND = not tested.
