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PDIA3 protein disulfide isomerase family A, member 3. Cytoplasm peptidase. P18669. PGAM1 phosphoglycerate mutase 1 (brain). Cytoplasm phosphatase.
Additional File 1 Supplementary Tables Supplementary Table S1 Proteins identified from a pituitary adenoma tissue with two-dimensional gel electrophoresis and mass spectrometry ID P04217 P32391

Notes Molecules A1BG ACTR3

P30566 P30566 P15121

D D

O43488 P02768 P02768 P02768 P02768 P04083 P07355 P09525 P08758 P08758 P02743 P02647 P02647 P06576

O75947

ADSL ADSL AKR1B1 AKR7A2

D D D D

D D D D

ALB ALB ALB ALB ANXA1 ANXA2 ANXA4 ANXA5 ANXA5 APCS APOA1 APOA1 ATP5B

ATP5H (includes EG:10476)

Description Location alpha-1-B glycoprotein Extracellular Space ARP3 actin-related protein 3 homolog Plasma Membrane (yeast) adenylosuccinate lyase Cytoplasm adenylosuccinate lyase Cytoplasm aldo-keto reductase family 1, member B1 Cytoplasm (aldose reductase) aldo-keto reductase family 7, member A2 Cytoplasm (aflatoxin aldehyde reductase) albumin Extracellular Space albumin Extracellular Space albumin Extracellular Space albumin Extracellular Space annexin A1 Plasma Membrane annexin A2 Plasma Membrane annexin A4 Plasma Membrane annexin A5 Plasma Membrane annexin A5 Plasma Membrane amyloid P component, serum Extracellular Space apolipoprotein A-I Extracellular Space Extracellular Space apolipoprotein A-I ATP synthase, H+ transporting, Cytoplasm mitochondrial F1 complex, beta polypeptide ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d

Cytoplasm

Function other other enzyme enzyme enzyme enzyme transporter transporter transporter transporter other other other other other other transporter transporter transporter

transporter

Drugs

sorbinil

P30043

BLVRB

P01024 P00915

C3 CA1 (includes EG:759)

P27797 P04632 P04040 P35520 P51946 P49368

CALR CAPNS1 CAT CBS CCNH CCT3

P29373 P32321 O00559

CRABP2 DCTD EBAG9

P30084

ECHS1

P10126

EEF1A1

P26641

EEF1G

P06733 P09104 P30040

ENO1 ENO2 ERP29

biliverdin reductase B (flavin reductase (NADPH)) complement component 3 carbonic anhydrase I

calreticulin calpain, small subunit 1 catalase cystathionine-beta-synthase cyclin H chaperonin containing TCP1, subunit 3 (gamma) cellular retinoic acid binding protein 2 dCMP deaminase estrogen receptor binding site associated, antigen, 9 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial eukaryotic translation elongation factor 1 alpha 1 eukaryotic translation elongation factor 1 gamma enolase 1, (alpha) enolase 2 (gamma, neuronal) endoplasmic reticulum protein 29

Cytoplasm

enzyme

Extracellular Space Cytoplasm

peptidase enzyme

Cytoplasm Cytoplasm Cytoplasm Cytoplasm Nucleus Cytoplasm

transcription regulator peptidase enzyme enzyme transcription regulator other

Cytoplasm Unknown Cytoplasm

transporter enzyme other

Cytoplasm

enzyme

Cytoplasm

translation regulator

Cytoplasm

translation regulator

Cytoplasm Cytoplasm Cytoplasm

transcription regulator enzyme transporter

methazolamide, hydrochlorothiazide, acetazolamide, trichloromethiazide, dorzolamide, chlorothiazide, dorzolamide/timolol, brinzolamide, chlorthalidone, benzthiazide, sulfacetamide, topiramate

P00742

Q9NRD0 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 P02679 Q14192 Q13451 P02794 P02792 P06241 P50395 P01241

D D D D D D D D D D D D D D D D D D

F10

coagulation factor X

Extracellular Space

peptidase

FBXO8 FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FGG FHL2 FKBP5 FTH1 FTL FYN

F-box protein 8 fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain fibrinogen gamma chain four and a half LIM domains 2 FK506 binding protein 5 ferritin, heavy polypeptide 1 ferritin, light polypeptide FYN oncogene related to SRC, FGR, YES

Unknown Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Nucleus Nucleus Cytoplasm Cytoplasm Plasma Membrane

other other other other other other other other other other other other other other other other other other other other enzyme enzyme other kinase

GDI2 GH1

GDP dissociation inhibitor 2 growth hormone 1

Cytoplasm Extracellular Space

other cytokine

antihemophilic factor, dalteparin, heparin, coagulation Factor VIIa, enoxaparin, coagulation factor IX, rivaroxaban, deligoparin, idraparinux, tifacogin, RPR 120844, RPR 208566, DPC 423, fondaparinux thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin thrombin

dasatinib

P09471

D

GNAO1

P30443

D

HLA-A

guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O guanine nucleotide binding protein (G protein), beta polypeptide 2 guanine nucleotide binding protein (G protein), beta polypeptide 3 gelsolin (amyloidosis, Finnish type) gelsolin (amyloidosis, Finnish type) glutathione S-transferase M2 (muscle) glutathione S-transferase omega 1 glutathione S-transferase omega 1 glutathione S-transferase pi 1 hydroxyacyl-Coenzyme A dehydrogenase/3 ketoacyl-Coenzyme A thiolase/enoylCoenzyme A hydratase (trifunctional protein), beta subunit major histocompatibility complex, class I, A

P29777

D

GNAO1

P30455

D

HLA-A

major histocompatibility complex, class I, A Plasma Membrane

HNRNPC

heterogeneous nuclear ribonucleoprotein C (C1/C2) hemopexin hemopexin hydroxysteroid (17-beta) dehydrogenase 10 heat shock transcription factor 1 heat shock transcription factor 1 heat shock 70kDa protein 5 (glucoseregulated protein, 78kDa) heat shock 70kDa protein 8 heat shock 70kDa protein 8 heat shock 27kDa protein 1

P11016

GNB2

P16520

GNB3

P06396 P06396 P28161 P78417 P78417 P09211 P55084

D D D D

P07910

GSN GSN GSTM2 GSTO1 GSTO1 GSTP1 HADHB

P02790 P02790 Q99714

D D

HPX HPX HSD17B10

Q00613 Q00613 P11021

D D

HSF1 HSF1 HSPA5

P11142 P11142 P04792

D D

HSPA8 HSPA8 HSPB1

Plasma Membrane

enzyme

Plasma Membrane

enzyme

Plasma Membrane

enzyme

Plasma Membrane

enzyme

Extracellular Space Extracellular Space Cytoplasm Cytoplasm Cytoplasm Cytoplasm Cytoplasm

other other enzyme enzyme enzyme enzyme enzyme

Plasma Membrane

Nucleus

transmembrane receptor transmembrane receptor other

Extracellular Space Extracellular Space Cytoplasm

transporter transporter enzyme

Nucleus Nucleus Cytoplasm

transcription regulator transcription regulator other

Cytoplasm Cytoplasm Cytoplasm

enzyme enzyme other

O75874

D

IDH1

O75874

D

IDH1

P50213

IDH3A

Q9P0V2

IMMT

Q9UBX7 P08727 P35527 P07195 O75198 P41279

D

P41279

D

P40926 P16475

D

P24572

D

P24844

P54920 O75489

P22392 O75665 O14753 O14753

D D

isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 3 (NAD+) alpha

inner membrane protein, mitochondrial (mitofilin) KLK11 kallikrein-related peptidase 11 KRT19 keratin 19 KRT9 keratin 9 (epidermolytic palmoplantar keratoderma) LDHB lactate dehydrogenase B LOC390688 CDC37-like MAP3K8 mitogen-activated protein kinase kinase kinase 8 MAP3K8 mitogen-activated protein kinase kinase kinase 8 MDH2 malate dehydrogenase 2, NAD (mitochondrial) MYL6 myosin, light chain 6, alkali, smooth muscle and non-muscle MYL6 myosin, light chain 6, alkali, smooth muscle and non-muscle MYL9 myosin, light chain 9, regulatory (includes EG:10398) NAPA N-ethylmaleimide-sensitive factor attachment protein, alpha NDUFS3 NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase) NME2 non-metastatic cells 2, protein (NM23B) expressed in OFD1 oral-facial-digital syndrome 1 OVOL1 ovo-like 1(Drosophila) OVOL1 ovo-like 1(Drosophila)

Cytoplasm

enzyme

Cytoplasm

enzyme

Cytoplasm

enzyme

Cytoplasm

other

Extracellular Space Cytoplasm Cytoplasm

peptidase other other

Cytoplasm Unknown Cytoplasm

enzyme other kinase

Cytoplasm

kinase

Cytoplasm

enzyme

Cytoplasm

other

Cytoplasm

other

Cytoplasm

other

Cytoplasm

transporter

Cytoplasm

enzyme

Nucleus

kinase

Plasma Membrane Nucleus Nucleus

other transcription regulator transcription regulator

P07237

P4HB

Q29459

Q15365

P30101 P18669 O95336 P35232

procollagen-proline, 2-oxoglutarate 4dioxygenase (proline 4-hydroxylase), beta polypeptide PAFAH1B2 platelet-activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa

Cytoplasm

enzyme

Cytoplasm

enzyme

PCBP1 (includes EG:5093) PDIA3

poly(rC) binding protein 1

Nucleus

translation regulator

protein disulfide isomerase family A, member 3 phosphoglycerate mutase 1 (brain) 6-phosphogluconolactonase prohibitin

Cytoplasm

peptidase

Cytoplasm Cytoplasm Nucleus

phosphatase enzyme transcription regulator

phosphatidylinositol transfer protein, alpha

Cytoplasm

transporter

Unknown

ion channel

Cytoplasm

enzyme

Q00169

PGAM1 PGLS PHB (includes EG:5245) PITPNA

Q9NTG1

PKDREJ

P05092

D

PPIA

polycystic kidney disease (polycystin) and REJ homolog (sperm receptor for egg jelly homolog, sea urchin) peptidylprolyl isomerase A (cyclophilin A)

P05092

D

PPIA

peptidylprolyl isomerase A (cyclophilin A)

Cytoplasm

enzyme

P32119 P32119 P30041 O43422

D D

PRDX2 PRDX2 PRDX6 PRKRIR

peroxiredoxin 2 peroxiredoxin 2 peroxiredoxin 6 protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) proteasome (prosome, macropain) 26S subunit, ATPase, 1 proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) proteasome (prosome, macropain) activator subunit 2 (PA28 beta)

Cytoplasm Cytoplasm Cytoplasm Nucleus

enzyme enzyme enzyme other

Nucleus

peptidase

Cytoplasm

other

Cytoplasm

peptidase

Q03527

PSMC1

Q06323

PSME1

Q9UL46

PSME2

N-methyl-4-Ilecyclosporin N-methyl-4-Ilecyclosporin

P09455 P05121

RBP1 SERPINE1

Q13435 P00441

SF3B2 SOD1

P30626 Q9P2R7

D

SRI SUCLA2

Q9P2R7

D

SUCLA2

D D

TF TPI1 TPM3 TPM3 TPM4 UCHL1

P02787 P00938 P06753 P06753 P07226 P09936 Q99536

VAT1

P45880 P12956

VDAC2 XRCC6

P31946

D

YWHAB

P31946

D

YWHAB

P42655

YWHAE

P27348

YWHAQ (includes EG:10971)

retinol binding protein 1, cellular serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 splicing factor 3b, subunit 2, 145kDa superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) sorcin succinate-CoA ligase, ADP-forming, beta subunit succinate-CoA ligase, ADP-forming, beta subunit transferrin triosephosphate isomerase 1 tropomyosin 3 tropomyosin 3 tropomyosin 4 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) vesicle amine transport protein 1 homolog (T. californica) voltage-dependent anion channel 2 X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa) tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, beta polypeptide tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, beta polypeptide tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, epsilon polypeptide tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, theta polypeptide

Extracellular Space Extracellular Space

transporter other

Nucleus Cytoplasm

other enzyme

Cytoplasm Cytoplasm

transporter enzyme

Cytoplasm

enzyme

Extracellular Space Cytoplasm Cytoplasm Cytoplasm Cytoplasm Cytoplasm

transporter enzyme other other other peptidase

Plasma Membrane

transporter

Cytoplasm Nucleus

ion channel enzyme

Cytoplasm

other

Cytoplasm

other

Cytoplasm

other

Cytoplasm

other

drotrecogin alfa

P08107 U gi2460318 U gi14249382 U P02023 U gi12805429 U P38607 U

Heat shock 70 kDa protein 1 RNA-binding protein regulatory subunit Hypothetical protein MGC15429 Hemoglobin beta chain Similar to DJ-1 protein Vacuolar ATP synthase catalytic subunit A, osteoclast isoform P10990 U Actin 15A Note: D = duplicate. U = unmapped. All IDs = 154. Mapped IDs = 147. Unmapped IDs = 7. Network eligible IDs = 106.

Supplementary Table S2 Differentially Expressed Proteins Identified From Human Pituitary Adenoma tissues with 2DGE-based Comparative Proteomics ID

Notes

Molecules

P49753 P15121

ACOT2 AKR1B1

FoldChange 5.8 14.6

P02647 P06576

APOA1 ATP5B

-3.2 5.0

P47756

D

CAPZB

6.5

CAPZB

D

CAPZB

6.5

P13987

CD59

-9.5

P12110 P14854

COL6A2 COX6B1

-14.7 9.3

Q14894 CSN1S1

-35.3 -17.8

P07108

CRYM CSN1S1 (includes EG:1446) DBI

Q9UHL4 Q14259

DPP7 ERH

-9.3 5.0

P30040

ERP29

4.6

4.3

Description

Location

Function

Drugs

acyl-CoA thioesterase 2 aldo-keto reductase family 1, member B1 (aldose reductase) apolipoprotein A-I ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide capping protein (actin filament) muscle Zline, beta capping protein (actin filament) muscle Zline, beta CD59 molecule, complement regulatory protein collagen, type VI, alpha 2 cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous) crystallin, mu casein alpha s1

Cytoplasm Cytoplasm

enzyme enzyme

orbinil

Extracellular Space Cytoplasm

transporter transporter

Cytoplasm

other

Cytoplasm

other

Plasma Membrane

other

Extracellular Space Cytoplasm

other enzyme

Cytoplasm Extracellular Space

enzyme other

Cytoplasm

other

Cytoplasm Nucleus

peptidase other

Cytoplasm

transporter

diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein) dipeptidyl-peptidase 7 enhancer of rudimentary homolog (Drosophila) endoplasmic reticulum protein 29

collagenase

P00742

F10

-83.0

P06241

FYN

3.9

GDI1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1 GH1

9.4 -180.6 -13.3 -13.3 -20.2 -14.1 -128.3 -14.8 -48.1 -17.3 -16.9 -88.8 -27.8 -51.2 -32.1 -478.6

P31150 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241 P01241

D D D D D D D D D D D D D D D

coagulation factor X

Extracellular Space

peptidase

antihemophili c factor, dalteparin, heparin, coagulation Factor VIIa, enoxaparin, coagulation factor IX, rivaroxaban, deligoparin, idraparinux, tifacogin, RPR 120844, RPR 208566, DPC 423, fondaparinux

FYN oncogene related to SRC, FGR, YES

Plasma Membrane

kinase

dasatinib

GDP dissociation inhibitor 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 1

Cytoplasm Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space

other cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine cytokine

P01241 P01241 P01241 P01242 Q04760 Q04760 P09471

P29777

D D D D D D

GH1 GH1 GH1 GH2 GLO1 GLO1 GNAO1

-13.4 -161.0 -37.0 -14.1 10.2 8.3 9.5

GPX4

-26.1 4.2 -3.1

-99.4

hemoglobin, beta

Cytoplasm

transporter

P02081 P14625

GSTM2 HBB (includes EG:3043) HBB (includes EG:3043) HBD HSP90B1

growth hormone 1 growth hormone 1 growth hormone 1 growth hormone 2 glyoxalase I glyoxalase I guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O glutathione peroxidase 4 (phospholipid hydroperoxidase) glutathione S-transferase M2 (muscle) hemoglobin, beta

D

GNAO1

9.5

-3.1 -11.0

hemoglobin, delta heat shock protein 90kDa beta (Grp94), member 1

Cytoplasm Cytoplasm

transporter other

P04792 Q9UJY1 O75874

HSPB1 HSPB8 IDH1

-5.1 -3.7 8.7

Cytoplasm Cytoplasm Cytoplasm

other kinase enzyme

P24592

IGFBP6

-20.9

heat shock 27kDa protein 1 heat shock 22kDa protein 8 isocitrate dehydrogenase 1 (NADP+), soluble insulin-like growth factor binding protein 6

Extracellular Space

other

IGLC1

IGLC1

-32.1

immunoglobulin lambda constant 1 (Mcg marker)

Cytoplasm

other

P36969 P28161 HBB

D

HBB

D

Extracellular Space Extracellular Space Extracellular Space Extracellular Space Cytoplasm Cytoplasm Plasma Membrane

cytokine cytokine cytokine other enzyme enzyme enzyme

Plasma Membrane

enzyme

Cytoplasm

enzyme

Cytoplasm Cytoplasm

enzyme transporter

17dimethylamin oethylamino17demethoxygel danamycin, IPI-504

IGLC3

IGLC3

-32.1

P08779

KRT16

-28.8

Q86U44 Q99542 P12524

METTL3 MMP19 MYCL1

-8.3 3.1 -17.8

O00217

NDUFS8

5.2

P20774 Q08752

OGN PPID

-38.0 5.1

Q00007

PPP2R2A

-8.2

PRL PRL PRL PRL PRL PRL SCGN

-99.9 -9.7 -26.2 -20.1 -36.7 -33.6 -6.6

Q9UI15 P21980

TAGLN3 TGM2

5.6 -17.1

Q8IWU9 O14530 P08670 P27348

TPH2 TXNDC9 VIM YWHAQ (includes EG:10971) ZNF266

10.6 -11.4 5.5 -43.5

P01236 P01236 P01236 P01236 P01236 P01236 O76038

Q14584

D D D D D D

7.3

immunoglobulin lambda constant 3 (KernOz+ marker) keratin 16 (focal non-epidermolytic palmoplantar keratoderma) methyltransferase like 3 matrix metallopeptidase 19 v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian) NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) osteoglycin peptidylprolyl isomerase D (cyclophilin D) protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha isoform prolactin prolactin prolactin prolactin prolactin prolactin secretagogin, EF-hand calcium binding protein transgelin 3 transglutaminase 2 (C polypeptide, proteinglutamine-gamma-glutamyltransferase) tryptophan hydroxylase 2 thioredoxin domain containing 9 vimentin tyrosine 3-monooxygenase/tryptophan 5monooxygenase activation protein, theta polypeptide (14-3-3 protein tau) zinc finger protein 266

Unknown

other

Cytoplasm

other

Nucleus Extracellular Space Nucleus

enzyme peptidase transcription regulator

Cytoplasm

enzyme

Extracellular Space Cytoplasm

growth factor enzyme

Cytoplasm

phosphatase

Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Extracellular Space Cytoplasm

cytokine cytokine cytokine cytokine cytokine cytokine other

Unknown Cytoplasm

other enzyme

Unknown Unknown Cytoplasm Cytoplasm

enzyme other other other

Nucleus

other

IL15-S21AA U P01620 IGLC2 P01621 P04433 HBB2 gi 1066765 HBB2 P01935 P01968 HBB2

U U U U U U U U U U

P40933-2

P01842

P18988 P18988

P18988

-2.7 -32.1 -32.1 -32.1 -32.1 -3.1 -176.7 -99.4 -99.4 -20.2 -20.2

Splice isoform IL15-S21AA of interleukin15 Ig kappa chain V-III region SIE Ig lambda chain C regions Ig Kappa chain V-III region NG9 Ig kappa chain V-III region VG Hemoglobin beta-2 chain (PANLE) Hemoglobin beta unit variant Hemoglobin beta-2 chain (PANLE) Hemoglobin alpha-3 chain (PANTR) Hemoglobin alpha-2 chain (BOSMU) Hemoglobin beta-2 chain (PANLE)

Note: D = duplicate. U = unmapped. All IDs = 86. Mapped IDs = 75. Unmapped IDs = 11. Network eligible IDs = 47.

Supplementary Table S3 Nitroproteins, and Proteins That Interact With Nitroproteins, Identified From Human Pituitary Adenoma Tissue With Nitrotyrosine Affinity Enrichment and Tandem Mass Spectrometry ID Q13017 P59901

Notes nY; NIE

Molecules ARHGAP5 LILRA4

O94892 P31321

nY; NIE nY

ZNF432 PRKAR1B

Description Rho GTPase activating protein 5 leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4 zinc finger protein 432 protein kinase, cAMP-dependent, regulatory, type I, beta

Location Cytoplasm Plasma Membrane

Function enzyme other

Nucleus Cytoplasm

other kinase

O95470 Q15027 P25787

nY nY nY

SGPL1 CENTB1 PSMA2

sphingosine-1-phosphate lyase 1 Cytoplasm centaurin, beta 1 Nucleus proteasome (prosome, macropain) subunit, alpha type, 2 Cytoplasm

Drugs

enzyme other peptidase

Q9UHA7 nY IL1F6 interleukin 1 family, member 6 (epsilon) Extracellular Space cytokine Q8IUC4 nY RHPN2 rhophilin, Rho GTPase binding protein 2 Cytoplasm other O43187 Y IRAK2 interleukin-1 receptor-associated kinase 2 Plasma Membrane kinase Q9C0E4 Y GRIP2 glutamate receptor interacting protein 2 Plasma Membrane other P62988 Y UBB ubiquitin B Cytoplasm other Note: nY = nitrotyrosine. Y = tyrosine. All IDs = 12. Mapped IDs = 12. Unmapped IDs = 0. Network eligible IDs = 10. NIE = Network illegibile.

Supplementary Table S4 Nitroproteins Identified From Human Pituitary Post-mortem Control Tissue With 2DGE-based Nitrotyrosine Western Blot and Tandem Mass Spectrometry ID P03996 P04270 P12718 P24071

Notes Molecules nY ACTA2 (includes EG:59) nY ACTC1 nY ACTG2 (includes EG:72) nY FCAR

Description actin, alpha 2, smooth muscle, aorta

Location Cytoplasm

Function other

actin, alpha, cardiac muscle 1 actin, gamma 2, smooth muscle, enteric

Cytoplasm Cytoplasm

other other

Fc fragment of IgA, receptor for

Plasma Membrane Q8IWL3 nY HSCB HscB iron-sulfur cluster co-chaperone homolog Cytoplasm Q6TCH7 nY PAQR3 progestin and adipoQ receptor family member III Cytoplasm Q13237 nY PRKG2 protein kinase, cGMP-dependent, type II Cytoplasm P25787 nY PSMA2 proteasome (prosome, macropain) subunit, alpha type, 2 Cytoplasm O60641 nY SNAP91 synaptosomal-associated protein, 91kDa homolog Plasma Membrane P52823 nY STC1 stanniocalcin 1 Extracellular Space Note: nY = nitrotyrosine. All IDs = 10. Mapped IDs = 10. Unmapped IDs = 0. Network eligible IDs = 9.

other other other kinase peptidase other kinase

Drugs

Supplementary Table S5 Clinical and Pathological Characteristics of Pituitary Adenomas and Controls Used in Comparative Proteomics Study Patient

Sex, Age

Clinical features/Tumor size

Immunohistochemistry

Reference

T164

M, 35

Nonfunctional, visual loss, 3 x 3.5 x 4 cm. Particial hypopituitarism

Neg.

[2]

T219

M, 68

Nonfunctional, 1.9 x 2.3 x 2.2 cm, invasion of the right cavernous sinus

Neg.

[2]

T165

M, 56

Nonfunctional, 3.2 cm in diameter craniocaudal

LH 2+

[2]

T208

F, 47

Nonfunctional, 2 x 2 x 2 cm

LH 1-2+

[2]

T237

F, 40

Nonfunctional, right cavernous sinus extension

LH 2+

[2]

T57

F, 59

Nonfunctional, 2 x 3 cm

FSH 1+

[2]

T77

M, 67

Nonfunctional, 2 x 2.2 x 2.4 cm, questionable cavernous sinus

FSH 2+

[2]

T89

M, 62

Nonfunctional, 2 x 2.3 x 2.3 cm

FSH 2+

[2]

T65

F, 54

Nonfunctional, 4 x 4 x 4 cm, cavernous sinus invasion

FSH 1+, LH 2+

[2]

T138

M, 60

Nonfunctional, 2.9 x 3.1 x 3.5 cm

FSH 2+, LH 2+

[2]

T185

M, 66

Nonfunctional, 2.8 x 2 x 2.4 cm, bilateral cavernous sinus invasion

FSH 2-3+, LH 2-3+

[2]

T87

M, 48

Prolactinoma with calcification

PRL+

[3]

T131

F, 52

Prolactinoma, serum 359 ng/ml, 2.5 x 3.5 x 2.8 cm

PRL 3+

[3]

T192

M, 41

Prolactinoma, serum PRL 1176 ng/ml, 3 x 2.5 x 2.0 cm

PRL 3+

[3]

T273

M, 36

Prolactinoma, serum PRL 1918 ng/ml, 2.0 x 2.1 x 2.5 cm

PRL 3+

[3]

C2

M, 27

Normal

ND

[2, 3]

C3

F, 40

Normal

ND

[2, 3]

C4

M, 45

Normal

ND

[2, 3]

C5

M, 36

Normal

ND

[2, 3]

C7

F, 34

Normal

ND

[2, 3]

C8

F

Normal

ND

[2, 3]

C9

M, 55

Normal

ND

[2, 3]

C10

F, 47

Normal

ND

[2, 3]

Adenomas were graded blindly by a neuropathologist from 0 to 4 for intensity of immunohistochemistry staining for each peptide hormone. Neg. = immunohistochemical stains for ACTH, LH, FSH, PRL, GH, and TSH were negative. LH+ = Nonfunctional (NF) adenoma that expressed leuteinizing hormone, or lutropin; FSH+ = NF that expressed follicle-stimulating hormone, or follitropin; FSH+, LH+ = NF that expressed both follicule-stimulating hormone and leuteinizing hormone; PRL+ = Prolactinoma that expressed prolactin. ND = not tested.