Additional file 4: List of primers used for microarray ...

Additional file 4: List of primers used for microarray validation. The primers efficiency in real-time PCR was calculated according to a dilution curve prepared for each primer. Gene

Primers

adh5 AF399909 adh8b AY309075 vtg1 NM_170767 vtg3 AF254638 efia L23807 cyp2k6 AF283813 raraa L03398 cyp1a AF057713 igf1 AF268051 esr1 AF349412 rdh10 BI890056 rdh14 BF718032 dhrs10 BI888372 scd BF717695 fads2 AF309556 star AF220435

GTCCCAAAGCTGGTGAACGAC AGCATGCATCAGGTCAAAGGC GTTCGGAGCCACAGATTTCC GTTGCCCACACACTCCAGAGA ACTACCAACTGGCTGCTTAC ACCATCGGCACAGATCTTC CAGATGGCTTTATCGGCGTGAC CACGGCAGGCCCATTGAAAC AATCGGTGGTGCTGGCAAGGTC AAGAACTCGCCGCAACCTTTG TGAGTGGGAAACGCCTGA GCCAAACTCTCTCCAATGCA ATGGATGACGCTGAGACGGGAC GTGTGGCCTTCTGTTCCTCACG GCAGGTTTTGACACTATCAGT GACTCCAGAAGCGGCAG CCTCGAGATGTATTGTGCG TACAGGAAGAGTGGCTATGC TAGTTGACGCATGTGCCA TAGGAGCGCTCACTCTGATC TTTGCCGTTTGATGCCATTGTG TCGTCGACTCCTTGCGTTGAGC TCCTGGCCTGCAGAGACATGAG CTGCAAAGAGGCCAGATCCAGC CAGGGTATTGGCAGGGCTTTCG TGCGCCACATCCTCAGCCTTTC GGATCACCGCGTGCATCACAAG TTCGAACCAGCAGCCAGCCAAC CAGCGGACACCTCAACTT AGCCAGAGCTCGCCAGA ACCTGTTTTCTGGCTGGGATG GGGTCCATTCTCAGCCCTTAC

Product size

Efficiency in real-time PCR

111 bp

E=1.99

113 bp

E=2

100 bp

E=2

112 bp

E=2

101 bp

E=2

126 bp

E=2

147 bp

E=1.8

160 bp

E=2

125 bp

E=1.8

123 bp

E=1.9

92 bp

E=2

110 bp

E=2

91 bp

E=2

101 bp

E=1.9

188 bp

E=1.8

81 bp

E=1.9