Additional File A1: multiple sequence alignment of full

Additional File A1: multiple sequence alignment of full-length Drosophila RD proteins.

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

AAAAAAAAAAAAAVAQGPGPQQ-SSNAT------------TASAIAINPAQS-----------LANTSTHSASSTGSSTP QVLAAAAAAAAAAAAATNVSQ-GNISTH------------SASSTGSSTPDN-----------NSSSNNNNNSSNSS--MHLTTTSSNSTASNANNNNNNNNNNNT-----------------------------TNNNNNS-----SSNNNNNSSQTN MHLTTTSGNSTASNANSNNNNN----------------------------------SNNNNNN-----TANNNNSSSSNN MHLTTTSSNSTASNANNNNNN-----------------------------------TANNNN------TSSNNNTTNTNG MHISAEVSSTTSNQIQQQQQQQQQQQQQQHQQL---------------HQQLHQQHQQQTTPTATTTTGGSTTKRRNAES MHISAEVSSTTSNQIQQQQQQHQQQ---HQQQQ---------------QQQQQQQQLLQQQQQQ--TATTTTTKRRNAES MHISAEVSSTTSNQTAHQQQQQQQQQHQQHQ-----------------QQQQQSTQQQLQQHTAATTTTGSTTKRRNAES MVTSSSTSPTGNGWSSSTGDFKGITALATATGG------GAGGATQGATASTGATTAEVLAVSSSASVGSSSPTGGASNG SMVTSSTSPTGNG-WSTPGDYKGATAATAPAAPSVV---TAATASTAAAAAAASVMCEVLPSSS-ASVGSSSPTGGASNG SMVTSSTSPTGTAGWTTTGDYKVAT----------------VGSGGSSSAATAVAMCDVLPVSSNASVGSSSPTGGASNG QVLAAAAAAAAAAAVVNSGSSGVSVAQSTSLAN------TSTHSASSSTGSS--------TPDISATANTTANSSSSSNS MHLTTTSSNSTASNANNNNNN-----------------------------------NTANNNN-----TSSNNNTTNTNG MHLTTTSTNSTASNANSNNNNN----------------------------------NNNNNNNNNSNNTANNNNSSSSNN MHLTTTSSNSTASNANNNNNN-----------------------------------TANNNN------TSSNNNTTNTNG MHISAEVSSTTSNQIQQ------------QQQQ---------------HQQQQQHQLLQHQQQQ--TATTTTTKRRNAES MHISAEVSSTTSNQIQQQQQQQQ---------L---------------HQQLHQQHQQQTTPTATTTTGGSTTKRRNAES MHISAEVSSTTSNQQQQHQQQQQ------QHNP---------------SATATAAAATTTTTSTTPITTGSNTKRRNAET AAAAAAAAAAAAAVAQGPGLQQ-SSNAASA----------TASASAINPAQS-----------LANTSTHSASSTGSSTP QVLAAAAAAAAAAAAATNVSQ-GNISTH------------SASSTGSSTPDNSHHSSSNNNNNNSSSTNNNNNSSSSNNN MVTSSSTSPTGNGWSSSTGDFKGITAVATATGG------GAGGATQGATASTGATVAEVMAVSSSASVGSSSPTGGASNG -MVTSSASPTGTG-WSSPGDYKSAS-----------------TAVAAAAVAAAAVMCEVLPSSS-ASVGSSSPTGGASNG SMVTSSTSPTGNG-WSTPGDYKGATAATAPAAPSVV---TAATASTAAAAAAASVMCEVLPSSS-ASVGSSSPTGGASNG TQATAAAAAAAAAVANTQVLQAAAAAAAAVAAANVSSASTTASSTGSSTPDN-INNSSNNNNNNNNNNSHSNNNNSSNNN MHLTTTSSNSTASNANNNNNN-----------------------------------TANNNNN-----TSSNNNTTNTNG MHLTTTSSNSTASNANSNNNNTNNN--------------------------------NNNNNN-----TANNNNTASSNN -------------------------------------------------------------------------------MHISAEVSSTTSNQIQQQHQQQQQQQQQQQQHQ---------------QQQQQQQQQHQQLQQQHQQTTNTTTKRRNAES MHISAEVSSTTSNQIQQQQ----------QQQQ---------------HQQQQQHQLLQQQQQQ--TATTTTTKRRNAES MHISAEVSSTTSNQTHQQQQQQHQQLQQQQH-----------------QQQQQVTQQQHTTATTTITTTSSTTKRRNVES MVTSSSTSPTGNGWSSSTGDFKGITAVATATGG------GASGATQGATASTGATAAEVLAVSSSASVGSSSPTGGASNG MVTSSSASPTGNGWTS-TGDFKGLTAVPAVVAA------TGASGGN----SAGGTTAKVLAISSSASVGSSSPTGGASNG -MVTSSASPTGTG-WSAPADYKSAT--------------TAAAAAAA----AAAVMCEVIPSSS-ASVGSSSPTGGASNG AAAAAAAAAAAAAVAQGPGPQQ-SNNVA------------TASAIAINPAQS-----------LANTSTHSASSTGSSTP SSHTQVLQAAAAAAAAAAAISG-------------------ASSAGSSAANN-----------QANTSTHSASSTGSSTP QVLAAAAAAAAAAAVVNSGSSGVSVAQSTSLAN------TSTHSASSSTGSS--------TPDISATANTTANSSSSSSS ---MANNNNNNSTTNNNNNST-----------------------------------TNNNNNS-----STANNNTSNTNG MHLTTTTSNTTSSTVNNNANN-----------------------------------NNNNNNTS--SNNSNNNNTSNTNG MHISAEVSSTTSNQIQQHQ----------QQQQ---------------HQLLQ-----QQQQQQ--TATTTTTKRRNAES MHISAEVSSTTSNQIHQQQQQQQQQQQ-HQQQQ---------------QQQQQQHQLLQQQQQQQQTATTTTTKRRNAES MHLTTTSSNSTASNANNNNNN-----------------------------------TANNNN------TSSNNNTTNTNG MHILAEVSSTTSNQTHQQQQQQQQQQQQQPT-----------------QQQQ-----HPAAATTTITTTASTTK-----AAAAAAAAAAAAAVAQGPGPQQ-SSNVA------------SASAIAINPAQS-----------LANTSTHSASSTGSSTP AAAAAAAAAAAAAVAQGPGPQQQSSNAATATAT------ATASAIAINPAQS-----------LANTSTHSASSTGSSTP MVTSSSTSPTGNGWSSSTGDFKGITAVATATGG------GVGGATQGATASTGATAAEVLAVSSSASVGSSSPTGGASNG -------------------------------------------------------------------------------QVLAAAAAAAAAAAAATNVGHPGNISTH------------SASSTGSSTPDN-------TNNNSSNTSSNNNNTSSSNNN AMVTSSASPTGTG-WSAPAEYKSAN--------------TAVAAATAGGSAAAAIMCEVLPSSS-ASVGSSSPTGGASNG

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

57 54 47 42 40 66 61 64 75 76 65 67 41 47 40 52 57 60 59 68 75 61 76 80 41 44 1 66 54 64 75 70 60 57 51 67 38 44 49 65 40 53 57 64 75 1 62 65

DLSTNNTSSSS-----------NATTSPQNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI --SSNNNNNNS----------NS---NAANL----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI GSNNNNNNT---------SSNNNNNSNNNNNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF NN-TSNTNG--------SNNNNSSSSNNN--TEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF SSNN--TSG----------NNNNSSGNN-SNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF SSNNNNNNSNN---NNNNNSTTNNNNNNNNNNNNNSSNNAKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV SASNNNNNTS----TTNNNNNNNNNNSTTNNNNNNNNN-VKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV NNNNNNNNNSN---SNNNN----NNSNNNNSNNNNTNSSSKNKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV TAHSGHSGHTGG-------HSSSTASNNNNIGASNSNSNSN--NNNNNAVHQDLLWMERLVQKRQQEHPGELVRTSNPYF TAHSGHSGGGNS---SSTTIATTTNNNNNNNNSNNSNNNNN-----NNAVHQDLLWMERLVLKRQQEHPGELVRTSNPYF TANSGHSGGGGG--------GNGGNSNSNSNNNSNNNNNNN-----NNAVHQDLLWMERYVAERQLEYPGELVRTSNPYF NSNTNTANSTA-----------NNGSSNSNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI SSNNNNTSG--------NNNNNNSSGNNNSNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF NNNTSNTNG--------SNNNNSSSSNNNNSTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF SSNNN-TSG----------NNNNSSGNN-SNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF SASSNNNNNNN-TSTTNNNNTNNNNNSTTNNNNNNNNNNVKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV SSNNNNNNSSS---NNNNN------NNSNNNNNNSSSNNAKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV NNNNNNNNNNN---------------------NNNNNSNAKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV DLSTNNTSSSS-----------NATTSPQNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI NTSSNNNNNSS----------NS---SNANA----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI TAHSGHSGHTGG-------HSSSSASHNNNNGASNSNSNSNSNNNNNNAVHQDLLWMERLVQKRQQEHPGELVRTSNPYF TANSAHSASGNK----------NNSNNNNNN---------------NNAVHQDLLWMERFVRERQQEYPGELVRTSNPYF TAHSGHSGGGQQ---QQHHYCHHHHQQHNNN-----NNNNN-----NNAVHQDLLWMERLVLKRQQEHPGELVRTSNPYF NNNTNSSSTSSSS--------ASSSSASSSS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI SSNNN-TSGGG-----NNNNNNNSSGNN-SNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF NNNNSNTNG---------SNNNNSSSSNNNNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF --------------------------------------------------MTSDILAERTLGDFLTEHPGELIRTSSPLF SSNNNNNNNTTNNNKNNNNNSNNNNNNNNTTNNNNNNNNVKTKAVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV SASSNNNNNN--TSTTNNNNTNNNNNSTTNNNNNNNN--VKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV NNNNNSTTNNN---NNNNN----NNNNNN---------SSKSKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV TAHSGHSGHTGG-------HSSSTASNSNNNGASNSN--------NNNAVHQDLLWMERLVQKRQQEHPGELVRTSNPYF TAHSGHSGST----------TSNSGNNNNNNSNSNNNSNSN---NNNNALHQDLKWMERLVMKRQQEHPGELVRTSNPYF TANSTHSGS-----------NKNNNNNNNNSRN--NNNNNS-----NNAVHQDLLWMERFVLERQQEYPGELVRTSNPYF DLSTNNTSSSS-----------NATTSPQNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI DLNTANISTSTTSSSSTASSANNNSSSNTNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI NS--NTANNTA-----------NNGSSNSNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI TNNTN---------------------TNNNNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF SNNNNNNNS--------SSSNNNNNNNNNNNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF SASSNNNNN-----------TNNNNNSTTNNNNNNNN--VKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV SASNNNNNNTS-TTNNNNTSNNNNNNNNSTTNNNNNNN-VKTKPVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHV SSNNN-TSG----------NNNNSSGNN-SNTEQNTPPTPAQLLNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLF --------------------------------------------LDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHI DLSTNNTSSSS-----------NATTSPQNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI DLSTNNTSSSS-----------NATTSPQNS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI TAHSGHSGHTGG-------HSSSTASNNNNNGASNSNSN------NNNAVHQDLLWMERLVQKRQQEHPGELVRTSNPYF -----------------------------------------------------------------------LVRTSNPYF SSNSNNNNSSS----------NTSAASAANS----------------AKMPSSMTDMFASLHEMLQEYHGELAQTGSPSI TANSANSGSGNKININNNNNNSSNNNNNNNNTNSRNNNNNS-----NNAVHQDLLWMERFVLERQQEYPGELVRTSNPYF

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

110 103 118 111 107 143 136 137 146 148 132 120 113 119 108 131 128 119 112 119 148 116 143 136 114 115 31 146 130 128 140 137 122 110 115 118 97 116 116 143 108 89 110 117 142 10 116 140

LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKESTQVD VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPSHWRSNKTLPLAFKVVALAEVGDGTYVTIRAGNDENCCAELRNCTAQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPSHWRSNKTLPLAFKVVALADVGDGTYVTIRAGNDENCCAELRNCTAQMKNCVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGR-------VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDL------SVGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGR-KDSTPVA VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPTHWRSNKTLPLAFKVVALADVGDGTYVTIRAGNDENCCAELRNYTAQMKNGVAKFNDLRLRGTQWSRNGSDRRR LCSALPSHWRSNKTLPLAFKVVALAEVGDGTYVTIRAGNDENCCAELRNCTAQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPSHWRSNKTLPLAFKVVALAEVGDGTYVTIRAGNDENCCAELRNCTAQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPSHWRSNKTLPLAFKVVALADVGDGTYVTIRAGNDENCCAELRNYTAQMKNGVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGASNTHWL VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTVLPPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI VCTTLPTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTI LCSALPNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLRFVGRSGRGKSFTLTI LCSALPSHWRSNKTLPLAFKVVALADVGDGTYVTIRAGNDENCCAELRNYTAQMKNGVAKFNDLRFVGRSGRGKSFTLTI

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

190 183 198 191 187 223 216 217 226 228 212 200 193 191 182 211 207 199 192 199 228 196 223 216 194 195 111 226 210 208 220 217 202 190 195 198 177 196 196 223 188 169 190 197 222 90 196 220

TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPAL TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQQAA-AAAVHHPAL TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A VIPTNQHEIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLP-----------------TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFPSTKALHELETLRRSAKVAAVTTAAAAA TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFP--KTLHELETLRRSAKVAAATTAAAAA TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYR-IGLTP----LGFP--KTLHELESLRRSAKVAAATTAVASV TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAA-QAAVHHPAL TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A ---------ATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A TVSTNPPHIATYNKAIKVTVDGPREPRSKTSKQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR VVPTNKNEIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPDVS--------------TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPAL TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQQAA-AAAVHHPAL TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFPSTKALHELETLRRSAKVAAVTTAAAAA RMDRLDQQRVDQAAEARLLISSQVAHQS-----------------------------IHKFLN-RQNGKLAEIAKQVADF TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFP--KTLHELETLRRSAKVAAATTAAAAA TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAA--AAVHHPAL TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFPSTKALHELETLRRSAKVAAVTTAAAAA TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFP--KSLHELEALRRTTK--AVTTAAAAA TVATSPPQVATYAKAIKVTVDGPREPRSKTSPPGGPQYRALGLGQRPFIDSFP--KTFHELETLRRSAKVAAATTAAAAA TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPAL TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPAL TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAA-QAAVHHPAL TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A SSCTTPTSAQFNNPALGFTCSSPMLPDSTASDLDQHLSSLVGSTSGQMTHHSLLGATGQTSISSTVNGASANVHSSQNGA VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR TVSTNPPHIATYNKAIKVTVDGPREPRSKT-RQQQQFHFAFGQRPFHFSTDPLSGFR-MPPIG--------NCQS----A VISTNPIQIATYTKAIKVTVDGPREPRSKV-RHQGFHPFAFG--PQRFGPDPLMAGLPFKLPGFAHHLVGMSHLHAPDWR TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPAL TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQAAAQAAQVHHPAL TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFPSTKALHELESLRRSAKVAAVTTAAAAA TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFPSTKALHELETLRRSAKVAAVTTAAAAA TIATYPVQIASYSKAIKVTVDGPREPRSKQS-YGYPHPGAFNPFMLNPAWLDAAYMTYGYADYFRHQQAA-AAAVHHPAL TVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPQYRALGLGQRPFIDGFP--KTFHELETLRHSAKVAAATTAAAAA

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

269 261 264 257 253 300 293 276 306 306 285 278 259 248 249 288 284 261 271 277 308 246 301 293 260 261 177 303 287 285 300 293 280 269 274 276 243 276 273 300 254 246 269 276 302 170 274 298

AKSSASSVSPNPNPSVATSS------------SSAVQP------SEYPHPAAAVAAAA------GQPAAMMPSPPGAAPA SKAATSPNGSASGVVSPGTA-------AAGAAVPAAAA------ADYPPPPSSVGVGVG----AVAPGSMMPSPPGGPPA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQTNNQDFGGATNRDCVP-MLPDSTA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA RYTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA AHMALGGRSAGFPAGPFFGHH-----AAFPAASGLRGLSADGQQQQQHQQQQ--HQQQLATVGAAHSTTSPEGSPTTTTS AHMALGGRPAAFTAAPFFGHH----AAAFPTASGLRGLSGD-----NQQHQQQQQ--QLATVGAAHSTTSPEGSPTTTTT -----------------------------------------------------DNQQQLPTVGAAHSTTSPEGSPTTTTS ATAASAANAVAVAAAAVAVTPTGGGVG---GVGGVAGGQGAGVGVGAGLVQQLSSNYSSP------NSTINSDCQVYKPN ATAATAATAVAAAAASVALPGSTTTTG---GVGVGVGVGGGSSGSGAGLVQQLSSNYSSP------NSTINSDCQVYKPN A------------AASVVTAAAVSTTSG--------------STSTTSLVQQLSSNYSSS------NSTINSDCPGYKPN SKASPSSSSSIVSPSASAAASSG-------AGNGAAGP------ADYHHVSQITPPPSRG-TNGRWPTGCHDALAAQEPL SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA SNTHWGYGSAASAYSPYLASSG--------LSSCPTPTSAQFHNPPLGFTCSSNDQSNNQDFGGATNRDCVPAVLPDSTA AHMALGGRPAAFTAAPFFGHH----AAAFPTASGLRGLSGD-----SQQHQQQQQQHQLATVGAAHSTTSPEGSPTTTTT AHMALGGRSAGFPAGPFFGHH-----AAFPAASGLRGLSADGQQQQQHQQQQQQHQQQLATVGAAHSTTSPEGSPTTTTS -----------------------------------------------QEQQQQQQHQHLATVGPAHSTTSPEGSPTATPT AKSSASSVSPNPNPSVATSS------------SSAVQP------SEYPHPAAAVAAAAAA----GQPAAMMPSPPGAAPA SKAATSPNGSNSGVISPGAAGGAAMPTAASAVVAAAAA------ADYPPVPPVVGVPVGG---VVPPASMMPSPPGGPPA ATAASAANAVAAAAAAVAVAPAGGGVA-----GGVAGG------AGAGLVPQLSSNYSSP------NSTINSDCQVYKPN E----------------------RESN--------------------ALTEELGEVPKSQ------LCPL-ADWRAWLSQ ATAATAATAVAAAAASVALPGSTTTGGVGVGVGVGVGVGGGSSGSGAGLVQQLSSNYSSP------NSTINSDCQVYKPN AKTSPGGGAPPPSGGGGAAP---------------TPP-------DYHHVSQLTPPPAG------APSNQPATGSATALA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQTNNQDFSGATNRDCVP-MLPDSTA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA AHMALGGRPAGFPAGPFFGHHAAAAAAAFPTASGLRGLSAA-----DNQQQQQQQ---QQQQQLAHSTTSPEGSPTTTTT AHMALGGRPAAFTAAPFFGHH----AAAFPTASGLRGLSGD-----SQQHQQQQ---HLATVGAAHSTTSPEGSPTTTTT AHMALGGR-PGFPAGPFFAHH-----HGAAFPATAA----------SGIRGLSENQQPLATVGAAHSTTSPEGSPTTTTS ATAASAANAVAAAAAAVAVTPTGGGGG----AGGVAGG------AGAGLVQQLSSNYSSP------NSTINSDCQVYKPN ATAATAATAVAAAAAAVAVSPAGGGGG---GTVGVVGG------SGAGLVQQLNSNYSSP------NSTINSDCQVYKPN A-------------VSVASAGNTLTTN-------------------ASIAQQLGSNYSSS------NSTINSDCQGYKPN AKSSASSVSPNPNPSVATSS------------SSAVQP------SEYPHPAAAVAAAA------GQPAAMMPSPPGAAPA AKAAAAQVSPVSAGSNSIPT------------AASVGAGAPGAGNVAPPPTGGDYHSGGA---PGQPAAMMPSPPGAAPA SKASPSSSSSIVSPSASAAASSG-------AGNGAAGP------ADYHHVSQITPPPSGAPTAAGQPAAMMPSPPGAAPA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA AHMALGGRPAAFTAAPFFGHH----AAAFPTASGLRGLSGD-----SQQHQQQQQ--HLATVGAAHSTTSPEGSPTTTTT AHMALGGRPAAFTTAPFFGHH----AAAFPTAGGLRGLSGD-----SQQHQQQQQQQQLATVGAAHSTTSPEGSPTTTTT SNTHWGYGSAASAYSPYLASSG--------LSSCTTPTSAQFNNPALGFTCSSNDQSNNQDFGGATNRDCVP-MLPDSTA AHMALGGR-PGFPAGPFFAHH-----HGASFPGGAT----------GGLRGLSDNQQQMATVGAAHSTTSPEGSPTTTTS AKSSASSVSPNPNPSVATSS------------SSAVQP------SEYPHPAAAVAAAA------GQPAAMMPSPPGAAPA AKSSASSVSPNPNPSVATSS------------SSAVQP------AEYPHPAAAVAAAAAA---AGQPAAMMPSPPGAAPA ATAASAANAVAAAAAAVAVTPTGGGGGV--AAGGVAGG------AGAGLVQQLSSNYSSP------NSTINSDCQVYKPN ATAASAANAVAAAAAAVAVTPTGGGGG----AGGVAGG------AGAGLVQQLSSNYSSP------NSTINSDCQVYKPN SKAATSPNGSVSGVVSPAMP-------------NAGAS------SDYPPPMPSASSSSSS---IVPPASMMPSPPGGAPA A-------------VSVASAGNALTTN-------------------ASIAQQLGSNYSSS------NSTINSDCQGYKPN

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

325 324 335 328 324 373 362 303 377 377 333 344 330 319 321 359 359 294 329 348 371 277 375 345 331 332 248 375 355 349 364 358 322 325 339 343 314 347 342 371 325 310 325 335 368 234 332 340

--TPYAIPQFPFNHVAAAAAAKA---------ATPHAFHPYNFA--AAAGLRARN---AALHHQSEP------VHVSPAS --SAYAMPQFPFNHVAAAAAAAAHQNQ----KSTPHAFHPYNFA--AAAGLRARNAAAAALHHGGLSA--AEAAHMSPAS SDLDQHLSSLVGSTSGQMTHHSL------LGATGQTSISSTVNGASGGSAGGGAGGAGGGAGAAGAGAAGGGGSNSILVP SDLDQHLSSLVGSSSGQMSHHSL------LGASGQTSITSTVNGAGAGSGAT---------AGSATGAGGGGGANSILVP SDLDQHLSSLVGSTSGQMTHHSL------LGAGGQTSISSTVNGASGGGSAG--AGTAGGGAGSGGGAGGGAGGNSILVP GT----LSAFVQPAIHCPLGS---------PRSPPTILTSLQHDNNNN---SSNIDAGFESDSISVTGSPRKSISSPLTH SG--TQLSAFVQPPMTSSPP------------PVTSLQHDNNNNNSNNNNSSSHIDAGFESDSISVTGSPRKSLASPLTH AT----LSAFVQPPA-CNASA---------GGSPPLTLSSMQHHDNNNNNSSSNIDAGFESDSISVTGSPRKSICS-LTH APHIQGADMMGAGEWT-GSSSS-----------AAAYYHSHA-HHPHAHHAHAHLQ-----------HQMALPPPPPPPA APHIQETDLMGAGEWTGSSSS------------AAAYYHSHAHHPHAH---HAHAHAHAHAHAHHLQHQMALPPPPPPPA APHIQETE---SGEWTGSASSS---NVAYTVGVGVPYTHHHATH---HTHPHSHSHAAAAHHLQH-HHQMTLPPPPQPPA RQPPYAIPQFPFNHVAAAAAAAAQQQQQHHAKSTPHAFHPYNFA--AAAGLRARNAAAAVLHHGGDATG---ISHISPAS SDLDQHLSSLVGSTSGQMTHHSL------LGAGGQTSISSTVNGASGGGSAG--AGTAGGGAGSGGGAGGGAGGNSILVP SDLDQHLSSLVGSTSGQMSHHSL------LGASGQTSITSTVNGASAGSGAT---------AGSAAGAGSGGGGNSILVP SDLDQHLSSLVGSTSGQMTHHSL------LGAGGQTSISSTVNGASGGGSAG--AGTAGGGAGSGGGAGGGAGGNSILVP SG--TQLSAFVQPPMTSSPP------------PVTSLQHDNNNNNSNNNNSSSHIDAGFESDSISVTGSPRKSLGSPLTH GT----LSAFVQPAIHCPLGS---------PRSPPTILTSLQHDNNNN---SSNIDAGFESDSISVTGSPRKSISSPLTH TT-TTTSSAFVQPNSGSSLGLGLGVGVVGLGAGSPTVLTSLSSQDDNNN--HNIDDAGFESDSISVTGSPRKSLSS-LTQ --TPYAMPQFPFNHVAAAAAAKA---------ATPHAFHPYNFA--AAAGLRARN---AALHHQSEP------VHVSPAS --SAYAMPQFPFNHVAAAAAAAAHQSQ----KATPHAFHPYNFA--AAAGLRARNAAAAALHHGGLSG--AETAHMSPAS APHIQGADMMGAGEWT-GSSSS-----------AAAYYHSHA-HHP-----HAHLQ-----------HQMALPPPPPPPA ALSVLQTQ-----------------------------------------------------------------------APHIQETDLMGAGEWTGSSSS------------AAAYYHSHAHHPHAHHA-HAHAHAHAHAHAHHLQHQMALPPPPPPPA -----STPQQPQHHHPAASSGMMP--------SPPPGAAAVPVP------------------------------ATAAAA SDLDQHLSSLVGSTSGQMTHHSL------LGAGGQTSISSTVNGASGGGSAG--AGTAGGGAGSGGGAGGGAGGNSILVP SDLDQHLNSLP--------------------------------------------------------------------SDLDQHLSSLVGSTSGQMTHHSL------LGATGQTSISSTVNGASAGSAGT--GGTGAGGAGS-----GGAGGNSILVP TSGHTQLSAFVQPPIGCSSPV---------LAPLTSLQ-DNENDNNNNTNNNSNIDAGFESDSISVTGSPRKSLGSPLTH SG--TQLSAFVQPPMTSSPP------------PVTSLQHDNNNNNSNNNNSSSHIDAGFESDSISVTGSPRKSLGSPLTH AT----LSAFVQPAA-CNASV---------GSSPPLTLSSMQHHDNNNNNSSSNIDAGFESDSISVTGSPRKSISS-LTH APHIQAAEMMGAGEWTNGSSSS-----------AAAYYHSHA-HHPHAHHAHAHLQ-----------HQMALPPPPPPPA APHIQETDLMGAGEWT-GSTST-----------AAAYYHSHAHHHPHAHHPHAHPHA------HLHQHQMALPPPPPPPA APQIQETDLIGAAEWTGSASSGANVAYPVGVGVGVPYHHAHHSHTHSHTHGHSAHH----HHAHHLQHQVALPPPPPPPA --TPYAIPQFPFNHVAAAAAAKA---------ATPHAFHPYNFA--AAAGLRARN---AALHHQSEP------VHVSPAS --AAYAMPQFPFNHVAAAAAAAAHQHP----KATPHAFHPYNFATAAAVGLRARN---AALHHQSEAGVVAPLNHVSPAS --AAYAIPQFPFNHVAAAAAAAAQQQQQHHAKSTPHAFHPYNFA--AAAGLRARNAAAAVLHHGGDATG---ISHISPAS SDLDQHLSSLVGSTSGQMTHHSL------LGATGQTSISSTVNGASAGSAGT--GGTGAGGAGS-----GGAGGNSILVP SDLDQHLSSLVGSTSGQMTHHSL------LGATGQTSISSTVNGASAGGATAG-SVTGGGTAGTGGSGGGGGATNSILVP SG--TQLSAFVQPPMTSSPP------------PVTSLQHDNNNNNSNNNNSSSHIDAGFESDSISVTGSPRKSLGSPLTH SG--TQLSAFVQPPMTSSPP------------PVTSLQHDNNNNNNGNNNISSHIDAGFESDSISVTGSPRKSLGSPLTH SDLDQHLSSLVGSTSGQMTHHSL------LGAGGQTSISSTVNGASGGGSAG--AGTAGGGAGSGGGAGGGAGGNSILVP AT----LSAFVQPPA-CNASA---------GGSPPLMLSSLQHHDNNNNNSSSNIDAGFESDSISVTGSPRKSISS-LTH --TPYAMPQFPFNHVAAAAAAKA---------ATPHAFHPYNFA--AAAGLRARN---AALHHQSEP------VHVSPAS --TPYAMPQFPFNHVAAAAAAKA---------ATPHAFHPYNFA--AAAGLRARN---AALHHQSEP------VHVSPAS APHIQAAEMMGAGEWTNGSSSS-----------AAAYYHSHA-HHPHAHHAHAHLQ-----------HQMALPPPPPPPA APHIQDLIQFPR----VSKAST-----------TTAFFAIT----------------------------------------AAYAMPQFPFNHVAAAAAAAAHQSQ----KSTPHAFHPYNFA--AAAGLRARNAAAAALHHGGVP---ADAAHMSPAS APQIQETDLIGAAEWTGSASSGANIAYPVGVGVGVPYHHAHHTHSHSHSHSHSHSHSHAAHHHHHLQHQVALPPPPPPPA

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

383 394 409 393 396 437 428 368 433 442 403 419 402 384 393 425 423 370 387 418 422 285 442 382 403 343 315 445 421 414 421 420 398 383 410 416 381 420 408 437 397 375 383 393 425

SRPSSSSPT-------------------------------------------------------------QQHVLLKLNT SRPSSSSPT--PTPTS------------------------------------------------------MPHVLLKLNT RYHTNTSN-EYNVHSSQN----------GPRSLSDSSQAESPVQEDLLSTNTPNLGS--------------TGAAGANGG RYHTNASN-EYNVHSSQN----------GPRSLSDSSQAESPVQEDLLSTNTPNLGS---------------GSGAGASN RYHTNASN-EYNVHSSQN----------GPRSLSDSSQAESPVQEDLLTTNTPNLGS--------------TAGGGAANG DEEEAEADAEAEAEAEAEAEPD-----GEAEAEAGTQLSGDARSLATISGRSAAGSL-------TETAPGSGGAFTALIQ DEE--EAEAEAEADADAEA---------EEAEVGGLSLNGGGIQGTLHSESSPGS----------------GGAFTALIQ DEEEPDP--------------------EAGRSRSPTLLSADASSVATLADPAPGS----------------GGAFTALIQ AAPVSVGVGGNGATMG--MGMGVG----MGMNHYG---GGYDSANSLEAGQYAA---------------HLPAVLPEMHG AAPVTNGAG--GMGVGVGMGVGMGMGVGMSMNHYG---SGYETASSLDAGNYA----------------HLPAVLPEMHG ATPVAMGGQASGMGMGMGMGMGMGMSMGMTMNHYGSTMGGYDASY-----DTS----------------HLPTVLQDMHG SRPSSSSPT-------------------------------------------------------------QQHVLLKLNT RYHTNASN-EYNVHSSQNXXXXVHSSQNGPRSLSDSSQAESPVQEDLLTTNTPNLGS--------------TAGGGAANG RYHTNASN-EYNVHSSQN----------GPRSLSDSSQAESPVQEDLLSTNTPNLGS---------------GSGGGATN RYHTNASN-EYNVHSSQN----------GPRSLSDSSQAESPVQEDLLTTNTPNLGS--------------TAGGGAANG DEEEAEAEAEAEAEAEAEA---------EEAEVGGLSRNGGGIQGPLHSESSPGS----------------GGAFTALIQ DEEEAEADAEAEAEAEAEAEPD-----GEAEAEPGTQLSGDARSLATISGRS---------------------------EDE--------------------------EADADGDGTEAESTLLGHQTEFSSGS----------------GGAFTALIQ SRPSSSSPT-------------------------------------------------------------QQHVLLKLNT SRPSSSSPT--PTPTS------------------------------------------------------MPHVLLKLNT AAPVSVGVGGNGATMG--MGMGMGVGMGMGMNHYG---GGYDSANSLEAGQYAA---------------HLPTVLPEMHG ------------------------------------------------------------------------AKYLELHS AAPVTNGAGGMGVGVGVGMGVGMGMGVGMSMNHYG---SGYETASSLDAGNYA----------------HLPAVLPEMHG AAAAMAMPQ------------------------------------------------------------------FPFNRYHTNASN-EYNVHSSQN----------GPRSLSDSSQAESPVQEDLLTTNTPNLGS--------------TAGGGAANG -------------------------------------------------------------------------------RYHTNASN-DYNVHSSQN----------GPRSLSDSSQAESPVQEDLLSTNTPNLGSG-------------TGGGGGAAN DEE----------ETDSIS---------REENTN----HNNGMAGSLG-ESSPGS----------------GGAFTALIQ DEE--EAEAEAEAEAEAEA---------EEAEVGGLSLNGGGIQGTLHSESSPGS----------------GGAFTALIQ DDEESQPNLDPEAETDAEA--------EAGRSRSPTLLSADASSVATPTEPAPGS----------------GGAFTALIQ AAPVSVGVGGNGATMG--MGMGVGV--GMGMNHYG---GGYDSANSLEAGQYAA---------------HLPAVLPEMHG AAPVSV--TGNGATMG--VGMGMGVGVGMGMGAMNP-YGGYDATNSLEAGNYAA---------------HLPAVLPEMHG AA-----------------PVPVPVATASAMNHYSSAMGGYDTTT-LDAGNY-----------------HISSVLPDMHSRPSSSSPT-------------------------------------------------------------QQHVLLKLNT SRPSSSSPT-------------------------------------------------------------QQHVLLKLNT SRPSSSSPT-------------------------------------------------------------QQHVLLKLNT RYHTNASN-DYNVHSSQN----------GPRSLSDSSQAESPVQEDLLSTNTPNLGSG-------------TGGGGGTAN RYHTNASNNDYNVHSSQN----------GPRSLSDSSQAESPVQEDLLSTNTPNLGG--------------SGTGAANGG DEE--EAEAEAEAEAEAEA---------EEAEVGGLSLNGGGIQGTLHSESSPGS----------------GGAFTALIQ DEE--EAEAEAEAEAEAEA---------EEAEVGGLSLNGGGIQGPLHSESSPGS----------------GGAFTALIQ RYHTNASN-EYNVHSSQN----------GPRSLSDSSQAESPVQEDLLTTNTPNLGS--------------TAGGGAANG DEEEPDP--------------------EAGRSRSPTLLSADASSVATLVEPAPGAGSGALDGTGTSGSGGGGGAFTALIQ SRPSSSSPT-------------------------------------------------------------QQHVLLKLNT SRPSSSSPT-------------------------------------------------------------QQHVLLKLNT AAPVSVGVGGNGATMG--MGMGVGV--GMGMNHYG---GGYDSANSLEAGQYAA---------------HLPAVLPEMHG -------------------------------------------------------------------------------401 SRPSSSSPTPMPTPTA------------------------------------------------------TPHVLLKLNT 420 AAPV---------------PVPVPVGTASAMNHYSSAMSGYETTT-LDAANY-----------------HISTVLPEMH-

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

402 418 464 447 451 505 481 412 489 501 462 438 467 438 448 480 470 408 406 442 482 293 503 395 458 343 371 485 474 470 479 480 442 402 429 435 437 476 461 490 452 435 402 412 483

SIETSSIHEQSASDGDSDDE------------------------------------QIDVVKSEFDLDKSLD-------SIETSSIHEQSASDADSDDE------------------------------------QIDVVKSEYELDKSIDSTRSSP-AANAGGSGGAAGTGSSSGA----------------GGAAIPAGNP-SMLGANQNFPGIVNQAQHAS-ASAYGGGAGVGVG GGAANGAASAAGAGSGASA-----------------GASAGAGNP-AMLGANQNFPGIVNQAQHAS-ASAYGGGVGVG-GAGSNAGSGAAGSGAGGAG-----------------VASSAVGNP-AMLGANQNFPGIVNQAQHSA-ASAYGG--GTGVRS-KNPSELFGGFAAAGAN---------------HFAP-SHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW RSGKNPTELFGGFAGGAGGN--------------HFAHSGHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW RS-KNPSELFGGFAAAGAG---------------HFAPAAHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW HGFATDPYQTAG-YGGGN---------------TAGGS-------ASKSELDYGGGYNQAWSNGYQNY-QYGSCLATA-HGFATDPYQTAGGYGTGAGA--------------GKSDLENLGGGGYGAG---GYNNAAAWSNGYNNY-QYGNCSAS--A HGIATDPYQMAGGYGSASGNPG-----------KTTTELETLNVTGYGSGAGSGYNN--AWSNGYNNYPQYGSCSAVATG SIETSSIHEQSASDADSDDE------------------------------------QIDVVKSEYDLDKSIDSSRSSP-GAGSNAGSGAAGSGAGGAG-----------------GASSAVGNP-SMLGANQNFPGIVNQAQHSA-ASAYGG--GTGVGGAANGSGSAAVAGSVASS-----------------VASAGAGNP-GMLGTNQNFPGIVNQAQHAS-ASAYGGGVGVG-GAGSNAGSGAAGSGAGGAG-----------------GASSAVGNP-KGLGANQNFPGM---------------------RSGKNPTELFGGFAA-AGGN--------------HFAPSGHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW --------------------------------------------------LFMQSP-LLPQSSQWLYTQLYGSYSD-LPW RS-KNHTDLFGGFSANGSASGAAGGGGGAGNPFPSSTPHTPTFNPALAAQLFLQSPALLPQSSQWLYSQLYGSYTDHLPW SIETSSIHEQSASDGDSDDE------------------------------------QIDVVKSEFDLDKSLD-------SIETSSIHEQSASDADSDDE------------------------------------QIDVVKSEYELDKSIDSTRSSP-HGFATDPYQTAG-YGGGN---------------AAGGS-------ASKSELDYGGGYNQAWSNGYQNY-QYGSCLATA-HSLLPKLVQAST-------------------------------------------------------------------HGFATDPYQTAGGYGTGAGA--------------GKSDLENLGGGGYGAG---GYNNAAAWSNGYNNY-QYGNCSAS--A ------------HDADSDDE------------------------------------QIDVVKSEYDLDKSIDSTRSSP-GAGTNAGSGAAGSGAGGAG-----------------GAASAVGNP-SMLGANQNFPGIVNQAQHSA-ASAYGG--GTGV------------------------------------------------AGRQRNFPGIVNQAQHAS-ASAYGG--GVGVG GGAANGGAGAAGAGAGSASSA-------------GAAAAVGVGNPGAMLGANQNFPGIVNQAQHAS-ASAYGG--GAG-RS-KNPTDLFGGFAGAGSGN--------------HFPP-AHSFNPALAAQLFLQSP-LLPQSSQWLYSQLYGSYSD-LPW RSGKNPTELFGGFAG-AGGN--------------HFAPSGHSFNPAQAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW RS-KNPTELFGGFAAAGAAS--------------HFAPAAHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW HGFATDPYQTAG-YGGGN---------------TGGGS-------ASKSELDYGGSYNQAWSNGYQNY-QYGSCLATA-HGFATDPYQTAG-YATGTGGVA-----------TGGGGNAVGNAGGSKSELDYGGGYNQAWSNGYQNY-QYGSCSATS--GFSADPYQTAAASGYCGTS--------------SKSDLESIN-ASYGATP-AAYNNPAAWSNGYNNY-QYGSCSATA-A SIETSSIHEQSASDGDSDDE------------------------------------QIDVVKSEFDLDKSLD-------SIETSSIHEQSASDADSDDE------------------------------------QIDVVKSEYDLDKSIDSTRSSPPSIETSSIHEQSASDADSDDE------------------------------------QIDVVKSEYDLDKSIDSSRSSP-GGAANGGAGAAGGGAGSASSA-------------GAAAAVGVGNPGAMLGANQNFPGIVNQAQHAS-ASAYGG--GAG-SGATGGGTAGTGSGISSPG----------------SGAVGAGGVASMLVGSNQNFQAHVSQHVSASSAYVSGGGGGVGGG RSGKNPTELFGGFAG-AGGN--------------HFAPSGHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW RSGKNPTELFGGFAG-AGGN--------------HFAPSGHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSD-LPW GAGSNAGSGAAGSGAGGAG-----------------GASSAVGNP-AMLGANQNFPGIVNQAQHSA-ASAYGG--GTGVRS-KNPSELFGGFAAAGGG---------------HFAPTAHSFNPALAAQLFLQSP-LLPQSSQWLYTQLYGSYSE-LPW SIETSSIHEQSASDGDSDDE------------------------------------QIDVVKSEFDLDKSLD-------SIETSSIHEQSASDGDSDDE------------------------------------QIDVVKSEFDLDKSLD-------HGFATDPYQTAG-YGGGN---------------TGGGS-------ASKSELDYGGSYNQAWSNGYQNY-QYGSCLATA--------------------------------------------------------------------------------427 SIETSSIHEQSASDADSDDE------------------------------------QIDVVKSEYELDKSIDSTRSSP-466 -GFSADPYQTAAASGYCGTS--------------SKSDLETLN-ASYGATP-TAYNNAAAWSNGYNNY-QYGSCSATA-A

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

438 460 526 506 509 566 545 474 543 561 529 480 525 497 543 498 487 442 484 536 305 563 425 516 372 433 547 537 533 533 545 503 438 472 477 499 540 524 553 510 497 438 448 537 469 527

-------------------------------------VAPLRMRCDLKAPSAMKPLYHES---------GPGAVANSRQP ---------------------------------TQQISVPLRMRCDLKAPSALKPLYHET-------ASAAAAVAAPRQA VGVGVGGGHGS-AAAAAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ ----VGGGHGGGAGATAAG----------CNGSLYPAYLT-------------------------------------------VGGGHGN-AAAAAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ LRSAAAAAAASAVPAQVQAQAQTQAQPQ---DSATSLMSSGGDHDGVN--LIKRCVTLITH---NPPDAENANPNS--SP LRNAAAAAAAN-INPGQENAG-------------VPPLGSNPDHDGVN--LIKRCVTLITHXXXNPPDAENANPNA--SP LRSAAAAAAANTAPQQHQQQQQESG-------SVLPAAAN--DADGVN--LIKRCVTLITH---NPPDAENANPNA--SP QYGPQAAPPPQPPP--PPPVV--------LCPQLYSTVNQNQIHLHLHSSEKLEQYLGTATG---ADHLTIGSLTGSSRS QYGGTGGHAAAPPPPPPPPVV--------LYPQLYSTVNQNQIHLHLHSSEKLEQYLGSASD----QQLTISSLTGS-RS QYGGATAAAPPPP---PPPVV--------LYPQLYSTVNQNQIHLHLHSSEKLEQYLGTTAD----QQLTISSLTGN-RS --------------------------------LAQHISVPLRMRCDLKAPSALKPLFHES-----------AAAATNRQA ----VGGGHGS-AAAAAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ ----VAGGHGGGAGATTAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ -------------------------------------------------------------------------------LRNAAAAAAAN-INPGQENSG-------------IPPLGSNPDHDGVN--LIKRCVTLITH---NPPDAENANPNA--SP LRSAAAAAAASAVPAQVQAQAQTQAQPQ---DSATSLMSSGGDHDGVN--LIKRCVTLITH---NPPDAENANPNS--SP LRSAAAAAAASSSSSASSSQQQPLTTTLTTVPAASTPAGASGGAVAVASDLIKRCVTLITH---NPPDVENSNPNAAASP -------------------------------------VAPLRMRCDLKAPSAMKPLYHES---------GPGAVASSRQP ---------------------------------TQQISVPLRMRCDLKAPSALKPLYHESSAAA-AAAAAVAAVHAPRQA QYGPQAAPPPQPPP--PPPVV--------LCPQLYSTVNQNQIHLHLHSSEKLEQYLGTATG---AEHLTIGSLTGSSRS -----------------------------------VKQFKEDLQLAQHYEASFRMGLATAERR--PELLPMSMAIAN--QYGGTGGHSAAPPPPPPPPVV--------LYPQLYSTVNQNQIHLHLHSSEKLEQYLGSASD----QQLTISSLTGS-RS ---------------------------------TQQISMPLRMRCDLKAPSSLKPLLHES--------TSTSPTAGLLQT ----VGGGHGS-AAAAAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ VGVGVGGGHGSGAGATAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ ----VGGGHGS-AAAAAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ LRNAAAAAAASNLNPNTTPTP-------------EGVPPSSQDHDGVN--LIKRCVTLITH---NPPDAENANPNA--SP LRNAAAAAAAN-INPGQENPG-------------VPTLGSNPDHDGVN--LIKRCVTLITH---NPPDAENANPNA--SP LRSAAAAAAASTTPQQQQQQQQQQQQQQQLQDSVVPLVASG-DADGVN--LVKRCVTLITH---NPPDAENANPNA--SP QYGPQAAPPPQPPP--PPPVV--------LCPQLYSTVNQNQIHLHLHSSEKLEQYLGTATS---ADHLTIGSLTGSSRS QYGPQTAAAPPPPP--PPPVV--------LYPQLYSTVNQNQIHLHLHSSEKLEQYLGTAAGG--GEQLTISSLTGSSRS QYGGAGAHATAP----PPPVV--------LYPQLYSTVNQNQIHLHLHSSEKLEQYL--ATD----QQLTISSLASN-RS -------------------------------------VAPLRMRCDLKAPSAMKPLYHES---------GPGAVANSRQP --------------------------------VAHHHLGPLRMRCDLKAPSAMKPLYHEP---------SVVAVN-PRQA --------------------------------LAQHISVPLRMRCDLKAPSALKPLFHES-----------AAAATNRQA ----VGGGHGS-AAAAAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ GGGGGGGGGGGGGGSGAGGGNVQHGSTN-GGGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLAAAAHSPNSSVHGAELQ LRNAAAAAAAN-INPGQENPG-------------VPPVGSNPDHDGVN--LIKRCVTLITH---NPPDAENANPNA--SP LRNAAAAAAAN-INPGQENHG-------------APPLGSNPDHDGVN--LIKRCVTLITH---NPPDAENANPNA--SP ----VGGGHGS-AAAAAAG----------CNGSLYPVLPASLLYSQLYTAANQSAHGFHSHTLP-AHASPNSSVHG-ELQ LRSAAAAAAASSTAAPQQQPQQQQSLPQQHEPSMLAAAAAGADADGVN--LIKRCVTLITH---NPPDAENANPNA--SP -------------------------------------VAPLRMRCDLKAPSAMKPLYHES---------GPGAVANSRQP -------------------------------------VAPLRMRCDLKAPSAMKPLYHES---------GPGAVASSRQP QYGPQAAPPPQPPP--PPPVV--------LCPQLYSTVNQNQIHLHLHSSEKLEQYLGTATS---ADHLTIGSLTGSSRS ----------------------------------------------------------------------------------------------------------------THHISVPLRMRCDLKAPSALKPLYHETSAAA-AAAAAAVAAVVPRQA QYGGAAGHATAP----PPPVV--------LYPQLYSTVNQNQIHLHLHSSEKLEQYL--ATD----QQLTISSLASN-RS

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398

472 SPETT--------------------------------------------------------------------------500 SPETTLP------------------------------------------------------------------------593 SVMDHISNVGV------RQQHNIM---AGGGVTHPGDLTLIGNCGASVRNIEDGNS------------------------------------------------------------------------------------------------------572 SVMDHISNVGV------RQQHNIM---AGGGVTHPGDLTLIGNCGASVRNIEDGNS-----------------------636 PVSSTRRSPSPS-------------------PVETIDLDDVSTTSRSASGSASG-------------------------607 PVSSTRRSPSP---------------------VETIDLDDVSTTSRSASGSSGL-------------------------G 538 PVSSSRRSPSP---------------------VETIDLDDVSTTSRSASGSSG--------------------------610 SIEIGQD--------------------QYHQQVHHAQQQQQQQQQQ--VHHPQQQQQQ---------QVESAGEVG---628 SIEIGPAGGLLGLTGEQEQ-HQHQNQHQQQQQLQQQPLEQSSEASNQS-YHLSHHQQHPHQ------QQQQQQQQQ---593 SIEIGLGGGGGTVGGGVGG---AGALTVSSQDQDQQVAVSNEAVANQS-YHLASQEVQ----------QQQQQEVG---517 SPETTLP------------------------------------------------------------------------588 SVMDHISNVGV------RQQHNIM---AGGGVTHPGDLTLIGNCGASVRNIEDGNS-----------------------561 SVMDHISNVGV------RQQHNIM---AGGGVAHPGDLTLIGNCGASVRNIEDGNT------------------------------------------------------------------------------------------------------602 PVSSTRRSPSP---------------------VETIDLDDVSTTSRSASGSS---------------------------G 568 PVSSTRRSPSPS-------------------PVETIDLDDVSTTSRSASGSASG-------------------------564 PVSSTRRSPSP---------------------VETIDLDDVSTTSRSASVSGSS-------------------------476 SPETT--------------------------------------------------------------------------530 SPETTLP------------------------------------------------------------------------603 SIEIGQD--------------------QYHQQVHHSQQQQQQQQQ---VHHPQQQQQQ---------QVESAGEVG----------------------------------------------------------------------------------630 SIEIGPAGGLLGLTGEQEH-HQHQ-QQQQQQHLQQQPLEQSSEASNQS-YHLSHHQQHPHQ------QQQQQQQQQQQ-464 SPETTSLT-----------------------------------------------------------------------579 SVMDHISNVGV------RQQHNIM---AGGGVTHPGDLTLIGNCGASVRNIEDGNS-----------------------440 SVMDHISNVGV------RQQHNIM---AGGGVAHPGDLTLIGNCGASVRNIEDGNT-----------------------496 SVMDHISNVGV------RQQHNIM---AGGGVTHPGDLTLIGNCGASVRNIEDGNS-----------------------607 PVSSSRRSPSP---------------------VETIDLDDVSTTSRSASGASSGEAGGGASG-----------------G 596 PVSSTRRSPSP---------------------VETIDLDDVSTTSRSASGSS---------------------------G 605 PVSSTRRSPSP---------------------VETIDLDDVSTTSRSASGSSG--------------------------600 SIEIGQD--------------------QYHQQVHHAQQQQQQQ-----VHHPQQQQ------------VESAGEVG---613 SIEIAQD--------------------PYQ---HHQQQQHHQHP------HPQQTEQQ---------VVPGGGGTG---564 SIEIGLGTGVCGGGGGGSSVSGGVALGEQEQQAAVSIAEQAAEGASQN-YHLHHHHH----------ETQRQMEAQ---472 SPETT--------------------------------------------------------------------------510 SPETTIP------------------------------------------------------------------------514 SPETTLP------------------------------------------------------------------------562 SVMDHISNVGV------RQQHNIM---AGGGVTHPGDLTLIGNCGASVRNIEDGNS-----------------------619 SVMDHISNVGVGVVRHQQHHHNIMSAAGGGGLTHPGDLTLIGNCGASVRNIEDGNVT----------------------583 PVSSTRRSPSP---------------------VETIDLDDVSTTSRSASGSS---------------------------G 612 PVSSTRRSPSP---------------------VETIDLDDVSTTSRSASGSS---------------------------G 573 SVMDHISNVGV------RQQHNIM---AGGGVTHPGDLTLIGNCGASVRNIEDGNS-----------------------570 PVSSSRRSPSP---------------------VETIDLDDVSTTSRSASGSSG--------------------------472 SPETT--------------------------------------------------------------------------482 SPETT--------------------------------------------------------------------------604 SIEIGQD--------------------QYHQQVHHAQQQQQQQQQQQQVHHPQQQQ------------VESAGEVG----------------------------------------------------------------------------------515 SPETTLP------------------------------------------------------------------------588 SIEIGLGTGLSGG----------VSLGEQDQQAAVAIAEQAAEGASQNNYHLHHHHHQQQQQQQQQLEAQRQLEAQHQQQ

DmeRun DvirGJ19252 DanaGF15983 DvirGJ18917* DseGM22662/63* DpseGA13742/42* DyakGE17890 DvirGJ19245/46* DyakGE17788 DpseGA22609 DwilGK25373 DperGL15806 DyakGE15340/41* DgriGH17774/75* DsimGD15530* DmeCG42267 DperGL15800/02* DwilGK19810/11* DereGG19698 DmojGI15491 DereGG18309 DgriGH11831* DperGL16499 DwilGK19815 DereGG17578 DmojGI11519 DpseGA25684 DanaGF15967 DsiGD17495 DgriGH17737/38* DseGM22478 DanaGF21771 DvirGJ18742 DseGM23050 DanaGF15970 DpseGA14956 DperGL15927 DwilGK20052/54* DseGM23044/45* DereGG19691 DmeCG34145 DmojGI15484/86* DsiGD17500 DyakGE17894 Dmelz DsiGD16951* DgriGH17740 DmojGI14398




477 ------------------TKIKSAAVQQKTVWRPY 507 ----------------AATKLKNAVVQQKTVWRPY 640 --------NRQVAALAAHRGHHNPTDNGGSVWRPY ----------------------------------619 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 671 -----------SGSSGGGPIRTRTPKPSADVWRPY 641 LGGGVGGMGGVGGAVG--PIRTRTPKPSADVWRPY 570 ---------------AGGPIRTRTPKPTADVWRPY 655 -------GSVAGGG--VESAREEDVGDLTQVWRPY 696 ----AVEQVQQGEGVGVEAPRDEDVGDLTQVWRPY 655 ---------------GEVSTRQED--DLTQVWRPY 524 ----------------AATKLKNSAVQQKTVWRPY 635 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 608 --------NRQVAALAAHRGHHNPTDNGGSVWRPY ----------------------------------634 HGGGVG----GGGAVG--PIRTRTPKPSADVWRPY 603 -----------SGSSGGGPIRTRTPKPSADVWRPY 597 ---------GGGPGSGGGPIRTRTPKPTADVWRPY 481 ------------------TKIKSAAVQQKTVWRPY 537 ----------------AATKLKNAVVQQKTVWRPY 647 -------GSGAGG---VESAREEDVGDLTQVWRPY ----------------------------------699 ----AVEQVQQGEGVGVEAPRDEDVGDLTQVWRPY 472 ---------------TAATKLKSAAVQQKTVWRPY 626 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 487 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 543 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 649 LAGGVGGAAAAAAVSGGGPIRNRTPKPSADVWRPY 628 HGGGVG----VGGAVG--PIRTRTPKPSADVWRPY 637 ---------------AGGPIRTRTPKPTSDVWRPY 639 -------GSGAGG---VESAREEDVGDLSQVWRPY 651 -------GGGGGSGDVVESPREEDVGDLTPVWRPY 629 --------QQHQSDVSNEAAREEDVGDMTQVWRPY 477 ------------------TKIKSAAVQQKTVWRPY 517 ----------------AATKLKSASVQQKTVWRPY 521 ----------------AATKLKNSAVQQKTVWRPY 609 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 676 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 615 HGGGVG----VGGAVG--PIRTRTPKPSADVWRPY 644 HGGGVG------GAVG--PIRTRTPKPSADVWRPY 620 --------NRQVAALAAHRGHHNPTDNGGSVWRPY 602 ---------------AGGPIRTRTPKPTADVWRPY 477 ------------------TKIKSAAVQQKTVWRPY 487 ------------------TKIKSAAVQQKTVWRPY 648 -------GSGAGG---VESAREEDVGDLSQVWRPY ----------------------------------522 ----------------AATKLKNAVVQQKTVWRPY 658 QQQQHQQQQQHPSDVSNEAAREEDVGDMTQVWRPY