CLUSTAL 2.1 MULTIPLE SEQUENCE ALIGNMENT File - PLOS

CLUSTAL 2.1 MULTIPLE SEQUENCE ALIGNMENT File: D:/New_LifeScience/collaborations/WangHongyan/GATA1 to GATA6/GATA345.ps Date: Fri Mar 23 22:31:26 2012 Page 1 of 3 GATA-4_Human ENSPTRG00000019993_Chimpanzee ENSBTAG00000005425_Cow ENSRNOG00000010708_Rat ENSMUSG00000021944_Mouse ENSGALG00000016662_Chicken ENSXETG00000000673_Frog ENSTRUG00000012112_J_Puffer ENSTNIG00000016369_T_Puffer ENSDARG00000035759_Zebrafish GATA-5_Human ENSPTRG00000013711_Chimpanzee ENSCAFG00000012709_Dog ENSMUSG00000015627_Mouse ENSRNOG00000036732_Rat ENSGALG00000005352_Chicken ENSTNIG00000002769_T_Puffer ENSDARG00000017821_Zebrafish GATA-6_Human ENSBTAG00000005734_Cow ENSMUSG00000005836_Mouse ENSRNOG00000023433_Rat ENSTRUG00000012674_J_Puffer ENSTNIG00000014837_T_Puffer ENSDARG00000035831_Zebrafish ENSXETG00000003144_Frog FBgn0003117_Fly

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MALTDGGWCLPKRFGAAG-ADASD-SRAFPAREPSTPPSPISSSSSS---CSRGGERGPGGASNCGTPQLDTEAAAGPPARSLLLSSYASHPFGAPHGPSAPGVAGPGGNLSSWEDLLLFTDLDQAATASKLLWSSR---GAKLSP ----MALTDGGWCLPKRLGAAPPADASD-SGAFPAREPSTPPSPISSSSSSC--CSRGGERGPGGASNCRTPQLDTEAAAGPTARSLLLSPYASHPFGAPHGPSAPGISGPAGALSSWEDLLLFADLDQAATASKLLWSSR---GAKLSP ----MALTDGGWCLPKRFGAAA-ADAGD-SGPFPAREPSSPLSPISSSSSS---CSRGGDRGPCGASNCRTPQLDAEAVAGPPGRSLLLSPYASHPFAAAHGAAAPGVAGPGSALSTWEDLLLFTDLDQAATASKLLWSSR---GAKLSP ----MALTDGGWCLPKRFGAAA-ADAGD-SGPFPAREPSSPLSPISSSSSS---CSRGGDRGPCGASNCRTPQLDTEAVAGPPGRSLLLSPYTSHPFAAAHGAAAPGVAGPGSALSTWEDLLLFTDLDQAATASKLLWSSR---GAKLSP -----------LLTPTLYGKCSWMDLGENSWSMVKREVSSS--PGSPAEQT---YLPGDSRRDGPTSDDLRTP-PSDLDALGHRRPDGRSLHSYVHFGHHN-----------NTLTTVEDIPLFTDLDQGG---KLVLPGG----AHKTG ---------------------SWMDLGENSWSMVKREVSSS--PGSPAEQS---YLPGDSRRDGPASDDLRTP-PSDLDALGHRRPDGRSLHSYVHFGHHN-----------NTLSTAEDIPLFTDLDQGG---KLVLPGG----AHKAG -----------------------MDLGDNSWSMVKREVSSSSSPGSPAEQS---YLNTDRR------DRLRSPAPTHLDPVGSRRAEGRPLHSYVHFGHPN-----------NALPSSDDVTLFTDLDQGN---KLIISGGHPRETHKGG MSWERHPQDSRLRTPISQRPHTWMDLNEDSWSGGTRLSPANSFFPCPIKQSPGTSPPPSPCSQLGSSECLRTEPDRGPHTIS---AEGRPAPSYHPLG----------------LSHPEDLILFRDLDHIN---KLIVPNR---ETRYSS -----------------------------------------------------------------------------------------------------------------------------------------------------1.......10........20........30........40........50........60........70........80........90.......100.......110.......120.......130.......140.......150

GATA-4_Human ENSPTRG00000019993_Chimpanzee ENSBTAG00000005425_Cow ENSRNOG00000010708_Rat ENSMUSG00000021944_Mouse ENSGALG00000016662_Chicken ENSXETG00000000673_Frog ENSTRUG00000012112_J_Puffer ENSTNIG00000016369_T_Puffer ENSDARG00000035759_Zebrafish GATA-5_Human ENSPTRG00000013711_Chimpanzee ENSCAFG00000012709_Dog ENSMUSG00000015627_Mouse ENSRNOG00000036732_Rat ENSGALG00000005352_Chicken ENSTNIG00000002769_T_Puffer ENSDARG00000017821_Zebrafish GATA-6_Human ENSBTAG00000005734_Cow ENSMUSG00000005836_Mouse ENSRNOG00000023433_Rat ENSTRUG00000012674_J_Puffer ENSTNIG00000014837_T_Puffer ENSDARG00000035831_Zebrafish ENSXETG00000003144_Frog FBgn0003117_Fly

: .. . .. * . ---------MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGA-------ASSPVYVPTPR-VPSSVLGLSY-LQGGGAGSASGGASGGSSGGAASGAGPGTQQGSPGWSQA-GADGAAYTP---------------------PPVSP------------MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGA-------ASSPVYVPTPR-VPFFVLGLSY-LQGGGAGSASGGASGGSSGGAASGAGPGTQQGSPGWSQA-GADGAAYTP---------------------PPVSP------------MYQSLAMAANHGPPPGPYEAGGPGAFMHGAGA-------ASSPVYVPTPR-VPSSVLGLSY-LQGGGGGAASGAASGGSSGAAPSGAGPGTQQGSPGWSQA-GAEGAAYTP---------------------PPVSP------------MYQSLAMAANHGPPPGAYEAGGPGAFMHSAGA-------ASSPVYVPTPR-VPSSVLGLSY-LQGGGSGAASGATSGGSSGAGPSGAGPGTQQGSPGWSQA-GAEGAAYTP---------------------PPVSP------------MYQSLAMAANHGPPPGAYEAGGPGAFMHSAGA-------ASSPVYVPTPR-VPSSVLGLSY-LQGGGSAAAAGTTSGGSSGAGPSGAGPGTQQGSPGWSQA-GAEGAAYTP---------------------PPVSP------------MYQSLAMAANPGPP--SYEGAG--GFMHGAAA-------AS-PVYVPTTR-VPSMLPSLPY-LPSSGSSQQASPVSSHS-----------------IWTQP-GAESAAYNPG---------------SS--HPPVSP------------MYQSIAMAANHGPP--AYEGAG--GYMHSATA-------ATSPVYVPTTR-VSSMIPSLPY-LQTSGSSQQGSPVSGHN-----------------MWAQA-GAEPSAYNPG---------------TS--HPPVSP------------MYQGI-MTSNHGPP--SYEAG----FLHNTSA-------ATSPVYVPTTRVVTPMIPALPY-LQTPGASQQSSPVSSHS-----------------AWAQP-GSDSVSSYS--------------------HSPVSP------------MYQGI-MSSSHGPP--SYEAG----FLHSTSA-------GTSPVYVPTTRVVTPMIPALPY-LQTPAASQQSSPASSHS-----------------AWTQP-GSEPVSSYS--------------------HSPVSP------------MYQGVTMAANHGAA--SYESG----FLHNS----------TSPVYVTPTR-VTPMIQALPY-LQAP---QQSSPASGHS-----------------GWAQP-GVETTSYNSS---------------TGHHHSPVS-------------MYQSLALAASPRQAAY-ADSGS---FLHAPG--------AGSPMFVPPAR-VP---SMLSY-LSGCEPSPQPPELA-----------------ARPGWAQTATADSSAFG-----------------PGSPHPPAAHPPGA ---------MYQSLALAASPRQAAY-ADSGS---FLHAPG--------AGSPMFVPPAR-VP---SMLSY-LSGCEPSPQPPELA-----------------ARPGWAQTATADSSAFG-----------------PGSPHPPAAHPPGA ---------MYPSLALAPSPGQAAY-TDSGA---FLPAPA--------AGSPVFVPPAR-VP---PMLPY-LPPCEPGPQAPGRG-----------------AHPGWAQ--AADSAPFG-----------------PGSPPPPAAPPPAA ---------MYQSLALAQSPGQGTY-ADSGA---FLHSSG--------TGSPVFVAPTR-MP---SMLPY-LPSCEPGSQAPALA-----------------AHSSWTQAVAADSSAFG-----------------SGSPHPPAAHPPGA ---------MYQSLALAQSPGQGTY-ADSGP---FLHSSG--------AGSPVFVAPTR-VP---SMLPY-LPSCEPGSQPPALA-----------------AHSSWTQAVSAESSAFG-----------------SGSPHPPATHPPGA ---------MYQGLALAPNHGQSAYSHDSGN---FLHSS---------AGSPVYVPTTR-VPSVLQTLPY-LQSCEP--HQSHLG-----------------NPPGWAQS-SGETTAFN-----------------AGSPHPPSG-------------MYQSLALSPN--QPPYPHDTGT---YLHPP---------ASSPVYVPSSR-VPAVLPTLPY-LQPCEAPPQSHGLGGHHGLGGHHG-----LGGHHGWPPA-SADGPPFP-----------------PSSPLPPHG-------------MYSSLALSSN--PSPYAHDSGN---YIHPS---------ASSPVYVPTTR-VPAMLQTLPY-LQTCESSHQAHGIS-----------------SHHAWPQT-GTDNSSFN-----------------PGSPHPPPG----FAPEQP-EEMYQTLAALSSQGPAAY-DGAPGG--FVHSAAAAAAAAAAASSPVYVPTTR-VGSMLPGLPYHLQGSGSGPANHAGG---------------AGAHPGWPQA-SADSPPYGSGGGAAGGGAAGPGGAGSAAAHVSAR----FAPEQP-EEMYQTLAALSS-GPAAY-DGSPGG--FVHSAAAAAAAAAAASSPVYVPTTR-ANKMLPGLPY-LQGAGGGPANHAGG---------------AGAHPGWPQA-SADSX---------------------------------FAAEQP-EEMYQTLAALSSQGPAAY-DGAPGG--FVHSAAAAAAAAAAASSPVYVPTTR-VGSMLSGLPY-LQGAGSGPSNHAGG---------------AGAHPGWSQA-SADSPPYG-GGGAAGGGAAGPGGAGSATAHASAR----FAAEQP-EEMYQTLAALSSQGPAAY-DGAPGG--FVHSAAAAAAAAAASS-PVKN-TTR-VGSMLPGLPY-LQGAGSGPSNHAGG---------------AGAHPGWPQA-SADSPPYG-GGGAAGGGAAGPGGAGSATAHASAR----LLVDP--TDMYQTLAIAAAQSQTGY-DSSSGG--YMHSNP---------NSPVYVPSSR-VGSMIPSLSY-LQAGGSAQPNHA-----------------VSSHSVWSQS-TTESPSYS-----------------TGSPHTSSR----LLVDP--TDMYQTLAIAAAQSQSGY-DSSPGG--YMHSNP---------NSPVYVPSSR-VGSMIPSLSY-LQAGG-AQPNHA-----------------VSSHSVWSQS-TTESPSYS-----------------TGSPHASSR----LIVDP--TDMYQTLAIAAAQSQAGYNDTPSAG--YMHSNP---------TSPVYVPTSR-VGTMIPNLSY-LPPGVSTQPSHA-----------------VSSHSVWSQP-APESPSYS-----------------TGSPHTSNR----ALPEPPAGDMYQTLTITAAQGPLGY-DHSQGT--FMHSAA---------SSPVYVPTSR-VGSMLTSISY-LQGTGASQGAHS-----------------VNNH--WSQA-TSEGSSFS-----------------SSSPHTSSR-----------------MGILLSDGDSTSDQQSTRDYPHFSGDYQN---VTLSAASASTSASASATHVAAVKMYHSSAVAAYTDLAAAG----------------------SAASAGVGVGVSG-----------------------------.......160.......170.......180.......190.......200.......210.......220.......230.......240.......250.......260.......270.......280.......290.......300

138 140 138 138 115 105 104 125

106 106 106 106 106 88 89 84 84 82 90 90 88 90 90 85 97 85 262 233 260 258 209 198 199 219 82

CLUSTAL 2.1 MULTIPLE SEQUENCE ALIGNMENT File: D:/New_LifeScience/collaborations/WangHongyan/GATA1 to GATA6/GATA345.ps Date: Fri Mar 23 22:31:26 2012 Page 2 of 3 GATA-4_Human ENSPTRG00000019993_Chimpanzee ENSBTAG00000005425_Cow ENSRNOG00000010708_Rat ENSMUSG00000021944_Mouse ENSGALG00000016662_Chicken ENSXETG00000000673_Frog ENSTRUG00000012112_J_Puffer ENSTNIG00000016369_T_Puffer ENSDARG00000035759_Zebrafish GATA-5_Human ENSPTRG00000013711_Chimpanzee ENSCAFG00000012709_Dog ENSMUSG00000015627_Mouse ENSRNOG00000036732_Rat ENSGALG00000005352_Chicken ENSTNIG00000002769_T_Puffer ENSDARG00000017821_Zebrafish GATA-6_Human ENSBTAG00000005734_Cow ENSMUSG00000005836_Mouse ENSRNOG00000023433_Rat ENSTRUG00000012674_J_Puffer ENSTNIG00000014837_T_Puffer ENSDARG00000035831_Zebrafish ENSXETG00000003144_Frog FBgn0003117_Fly

: : . :: :.* -RFSFPGTTGSLAA--AAAAAAAREAAAYSSGGGAAGAGLAGREQYGR---------------------AGFAG----------SYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRA-NPAA-RHPN----LDMFDDFS-EGR -RFSFPGTTGSLAA--AAAAAAAREAAAY--------------------------------------------------------------------------AAAASAGPFDSPVLHFLPGRA-NPAA-RHPN----LDMFDDFS-EGR -RFSFPGTTGSLAA--AAAAAAAREAAAYSSGSGAAGAGLAGREQYGR---------------------AGFAG----------SYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRA-NPAA-RHPN----LDMFDDFS-EGR -RFSFPGTTGSLAA--AAAAAAAREAAAYSSSGGAAGAGLAGREQYGR---------------------PGFAG----------SYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRA-NPA--RHPNL---VDMFDDFS-EGR -RFSFPGTTGSLAA--AAAAAAAREAAAYGSGGGAAGAGLAGREQYGR---------------------PGFAG----------SYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRA-NPG--RHPNL---VDMFDDFS-EGR -RFSFSTSTPIPST--SSRDAAAAYSSSLSLSAN-------GREQYSR----------------------GFGS----------SYSSPYPAYVSP-----EMATTWTSSPFDSPMLHNLQSRG-TPAAARHANI---AEFFDDYS-EGR -RFTFSSSPPISAA--SNR--EVPYSSPLAISAN-------GREQYGR----------------------ALGA----------TYSSPYPAYMSP-----DMGATWTASPFDSSMLHNLQNRA-GPAASRHPNI----EFFDDFS-EGR -RFAFSTSPPLSSGVAAARETAASYTNALNISAN-------GREHYGP---------------------RSLGG----------SYHSPYTPYMSP-----NVGGTWPASPFDSPVLHGLQSGT-ATGAARHPN----IDLFDDYA-EGR -RFSFSTSPPLS-------ETAASYTNPLNISAN-------GREHYGS---------------------RSLGG----------SYHSPYTPYMSP-----NVGGTWPASPFDSPVLHGLQSGT-PTGARGTQTSFILIDLFDDFA-EGR -RFTFSTSPPLTSG-----RDTATYT---------------SREHFGS---------------------RALSG----------SYHSGYPAYVSP-----NIGASWTASHFDSSVLHSLQTGG-AAAAR-HPN----LEFFDDLG-EGR TAFPFAHSPSGPGSGGSAGGRDGSAYQGALLP----------REQFAA------------------PLGRPVGT----------SYSATYPAYVSP-DVAQS----WTAGPFDGSVLH--------GLPGRRPTFV--SDFLEEFPGEGR TAFPFAHSPSEPGSGGSAGGRDGSAYQGALLP----------REQFAA------------------PLGRPVGT----------SYSATYPAYVSP-DVAQS----WTAGPFDGSVLH--------GLPGRRPTFV--SDFLEEFPGEGR TAFPFPHSPSGPGGG--AGARDGGAYQGALLA----------REQYPA------------------PLGRPVGA----------SYPAAYPAYVSP-EGAPA----WTSGPFEGSVLHGLQSRP-AGLPGRRATFV--SDFLEELPGEGR TTFPFAHSPPGSGSGGSAGVRDGGAFQGALLA----------REQYPT------------------PLGRPMGA----------SYPTTYPAYMSS-DVAPS----WTSGAFDSSILHGLQARP-GGLPGRRTSFV--PDFLEEFPGEGR TAFPFAHSPSGSGSGGSAGVRDGAAFQSALLA----------REQYPT------------------ALGRPMSA----------SYPTTYPAYVSP-DVAPS----WTSGPFDSSILHGLQGRP-GGLPGRRTTFV--PDFLEEFPGEGR --FSYPHSPP----GSSPPGRDG-AYQGPLLLGGGG------REQYGN------------------ALVRSVNG----------SYSSPYPAYVTP-ELPPS----WTAGHFESSVLHSLQTRQ-AALPGRRSTF----EYLEEFPGDGR --FSYSHSPP----GSGPSGREA-GYQGALVLGNGAR-----ADAYGG------------------ALVRTFGG----------SYSSPY-AYMSP-EMATS---SWTPGPFESGVPK-NRKIR-PASLGSDRSL----NLMDDGSTEGR --FSYSHSPP----VSSSTGRDA-AYQNPLMLSNGGR-----ADQYGS------------------ALVRSVGG----------SYSSPYAAYMSP-EMATS----WTPGPFDGGMIG-LQGRQ-GTLPGRRSSI----DMLDDLPCEGR --FPYSPSPP----MANGAAREPGGYAAAGSGG---------AGGVSGGGSSLAAMGGREPQYSSLSAARPLNGTYHHHHHHHHHHPSPYSPYVGA-PLT---PA-WPAGPFETPVLHSLQSRAGAPLPVPRGPS---ADLLEDLS-ESR ----------------------------------------------------------------------------------HHHHPSAYSPYVGA-PLT---PA-WPAGPFETPVLHSLQSRAGAPLPVPRGPG---TDLLEDLP-ESR --FPYSPSPP----MANGAARDPGGYVAAGGTG---------AGSVSGGGGSLAAMGGREHQYSSLSAARPLNG----TYHHHHHHHPTYSPYMAA-PLT---PA-WPAGPFETPVLHSLQGRAGAPLPVPRGPS---TDLLEDLS-ESR --FPYSPSPP----MANGAARDPGGYVAAGGAG---------AGSVSGGGGSLAAMGGREHQYSSLSAARPLNG----TYHHHHHHHPTYSPYMGA-PLT---PA-WPAGPFETPVLHSLQSRAGAPLPVPRGPS---ADLLEDLS-ESR --FHYPPSPP----MNNGTPRDGGYTNTLNVGS---------RDQYG--------------------LSRPLSG----------TYASPYSPYVAP-QLSQLPSP-WGGGPFDNTMLHTLQSRG-APL-IRGPNGV--SDILDDMG-ESR --FHYPPSPP----MNNGTPRDGGYSNTLNVSS---------REQYG--------------------LSRPLSG----------TYASPYSPYVAP-QLSQLSSP-WGGGPFDNTMLHTLQSRG-APL-IRGPNGV--SDILDDMG-ESR --FHYSPSPP----MNNGTSRDTSYTTPLNVNS---------RDQY---------------------LSRPISG----------SYPSPYPPYVTP-QLSQLPAA-WPAGPFENSMLHSLQSRS-APLAIRGPNG----DLLEEMV-ESR --YHYSPSPP----MANGSTRDTGYSSSLTVSG---------RDQYTP-------------------LARPLNG----------SYGSPYTPYMAP-QLT---SA-WPAGPFDNTMLHSLQSRG-APINVRGAPG----DVLDELP-ESR -YHQQAVNAPVYVPSNRQYNHVAAHFGSAAAQN-----------------------------------AWTTEG-----------FGSAHAQFYSP----------------NAAVMMGSWRSAYDPSGFQRSSP--YESAMDFQFGEGR .......310.......320.......330.......340.......350.......360.......370.......380.......390.......400.......410.......420.......430.......440.......450

215 172 215 215 215 186 184 184 181 168 187 187 190 194 194 184 196 184 388 292 382 380 307 296 295 314 167

GATA-4_Human ENSPTRG00000019993_Chimpanzee ENSBTAG00000005425_Cow ENSRNOG00000010708_Rat ENSMUSG00000021944_Mouse ENSGALG00000016662_Chicken ENSXETG00000000673_Frog ENSTRUG00000012112_J_Puffer ENSTNIG00000016369_T_Puffer ENSDARG00000035759_Zebrafish GATA-5_Human ENSPTRG00000013711_Chimpanzee ENSCAFG00000012709_Dog ENSMUSG00000015627_Mouse ENSRNOG00000036732_Rat ENSGALG00000005352_Chicken ENSTNIG00000002769_T_Puffer ENSDARG00000017821_Zebrafish GATA-6_Human ENSBTAG00000005734_Cow ENSMUSG00000005836_Mouse ENSRNOG00000023433_Rat ENSTRUG00000012674_J_Puffer ENSTNIG00000014837_T_Puffer ENSDARG00000035831_Zebrafish ENSXETG00000003144_Frog FBgn0003117_Fly

******::.*****:*****:******** **** .*** *.:* ..:** **.*:** * .*** * * :*********** ***** :**: :*:.* *** *. . ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASS-NSSNATTSSS--E ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASS-NSSNATTSSS--E ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAGPSGSESLPPATSASS-SSSSVATSSS--E ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAGPPG-ESLPPSSGASS-NSSNATSSSSSSE ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAGPAG-ETLPPSSGASSGNSSNATSSSSSSE ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLFKPQRR---LSASRRVGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKTKTPAGPSSSESLTPTT-----SSTSSSSSATTTE ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNLSKSKTPTGQNSSDSLTPST-----SSTNSAG-----E ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCTNCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNLNKIQP--GTPGGEGTPVTP-------SSTPRTSAASE ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRR---LSASRRVGLSCTNCHTTTTTLRR-NAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNLNKTQP--GTPGAEGTPATP-------SSTPRTSAASE ECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLVKPQRR---LSASRRVGLSCTNCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNISKTKP--GSSEGQSAISAV-------NSSP-----TE ECVNCGALSTPLWRRDGTGHYLCNACGLYHKMNGVNRPLVRPQKR---LSSSRRAGLCCTNCHTTNTTLWRRNSEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPKTIAKARGSSGSTRNASASPSAVA---STDSSAATSKAKP ECVNCGALSTPLWRRDGTGHYLCNACGLYHKMNGVNRPLVRPQKR---LSSSRRAGLCCTNCHTTNTTLWRRNSEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPKTIAKARGSSGSTRNASASPSAVA---STDSSAATSKAKP ECVNCGALSTPLWRRDGTGHYLCNACGLYHKMNGVNRPLVRPQKR---LSSSRRAGLCCTNCHTTTTTLWRRNADGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPKSVVKTKSNSGGLGNGTASPPSVP---DPESPAATLKPKP ECVNCGALSTPLWRRDGTGHYLCNACGLYHKMNGVNRPLVRPQKR---LSSSRRSGLCCSNCHTATTTLWRRNSEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPKNPAKIKGSSGSTANTTASSPTLL---NSESSATTLKAES ECVNCGALSTPLWRRDGTGHYLCNACGLYHKMNGVNRPLVRPQKR---LSSSRRSGLCCSNCHTATTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPKNPAKIKGSSGSTANSTASSPTLL---NTESSATTLKAES ECVNCGAMSTPLWRKDGTGHYLCNACGLYHKMNGINRPLK-PQKR---LSSSRRAGLCCTNCHTTNTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPKNITKGKTSTGSTTSATNSPSSIT---NSDS-TVTLKSEP ECVNCGSVSTPLWRRDGTGHYLCNACGLYHKMNGSNRPLVRPQRR---PAPSRRAGLCCTNCGTSTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPK-WPKSRNAAGPGISDSSSPSSLA---PSGH-ASSIKSEP ECVNCGSISTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQKRL--QSTSRRAGLCCTNCHTSTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPK-MPKTKSSSGSTVSGATSPTSLP---VSEN-ASTIKSEP ECVNCGSIQTPLWRRDGTGHYLCNACGLYSKMNGLSRPLIKPQKR---VPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNINKSKTC--SGNSNNSIPMTPT---STSSN--SDDCSK ECVNCGSIQTPLWRRDGTGHYLCNACGLYSKMNGLSRPLIKPQKR---VPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNINKSKAC--SGNSNNSVPMTPT---STSSN--ADDCSK ECVNCGSIQTPLWRRDGTGHYLCNACGLYSKMNGLSRPLIKPQKR---VPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNINKSKAC--SGNSSGSVPMTPT---SSSSN--SDDCTK ECVNCGSIQTPLWRRDGTGHYLCNACGLYSKMNGLSRPLIKPQKR---VPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVFQPLSSQKESICWRKRVPDNIKKNKTD--SGNKSLPMCRTPT---RSAGN--TTSGDL ECVNCGSISTPLWRRDGTGHFLCNACGLYSKMNGLSRPLIKPQKR---TSTSRRIGLSCANCQTSTTTLWRRNAEGEPVCNACGLYTKLHGVPRPLAMKKEGIQTRKRKPKTLNKAKGSGISGNNS--VSMTPT---STSSS-NSEDCSK ECVNCGSISTPLWRRDGTGHFLCNACGLYSKMNGLSRPLIKPQKR---TSTSRRIGLSCANCQTSTTTLWRRNAEGEPVCNACGLYTKLHGVPRPLAMKKEGIQTRKRKPKTLNKTKGS--SGNNS--VSMTPT---STSSS-NSEDCSK ECVNCGSMSTPLWRRDGTGHFLCNACGLYSKMNGLSRPLIKPQKR---MSSSRRIGLSCANCQTSTTTLWRRNAEGEPVCNACGLYTKLHGVPRPLAMKKEGIQTRKRKPKSLSKVKGS--SGISS--VPMTPT---SSSSS-NSEECTK ECVNCGSVQTPLWRRDGTGHYLCNACGLYSKMNGLSRPLIKPQKR---VPSSRRIGLACANCHTTTTTLWRRNTEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNLNKSKSSSSNGNSSHQIPMTPT---STTSSTNSDDCIK ECVNCGAISTPLWRRDGTGHYLCNACGLYHKMNGMNRPLIKPSKRLVSATATRRMGLCCTNCGTRTTTLWRRNNDGEPVCNACGLYYKLHGVNRPLAMRKDGIQTRKRKPKKTGSGSAVGAGTGSGTGSTLEAIKECKEEHDLKPSLSLE .......460.......470.......480.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600

359 316 359 360 361 328 321 322 318 301 331 331 334 338 338 326 338 327 528 432 522 520 448 435 434 458 317

CLUSTAL 2.1 MULTIPLE SEQUENCE ALIGNMENT File: D:/New_LifeScience/collaborations/WangHongyan/GATA1 to GATA6/GATA345.ps Date: Fri Mar 23 22:31:26 2012 Page 3 of 3 GATA-4_Human ENSPTRG00000019993_Chimpanzee ENSBTAG00000005425_Cow ENSRNOG00000010708_Rat ENSMUSG00000021944_Mouse ENSGALG00000016662_Chicken ENSXETG00000000673_Frog ENSTRUG00000012112_J_Puffer ENSTNIG00000016369_T_Puffer ENSDARG00000035759_Zebrafish GATA-5_Human ENSPTRG00000013711_Chimpanzee ENSCAFG00000012709_Dog ENSMUSG00000015627_Mouse ENSRNOG00000036732_Rat ENSGALG00000005352_Chicken ENSTNIG00000002769_T_Puffer ENSDARG00000017821_Zebrafish GATA-6_Human ENSBTAG00000005734_Cow ENSMUSG00000005836_Mouse ENSRNOG00000023433_Rat ENSTRUG00000012674_J_Puffer ENSTNIG00000014837_T_Puffer ENSDARG00000035831_Zebrafish ENSXETG00000003144_Frog FBgn0003117_Fly

GATA-4_Human ENSPTRG00000019993_Chimpanzee ENSBTAG00000005425_Cow ENSRNOG00000010708_Rat ENSMUSG00000021944_Mouse ENSGALG00000016662_Chicken ENSXETG00000000673_Frog ENSTRUG00000012112_J_Puffer ENSTNIG00000016369_T_Puffer ENSDARG00000035759_Zebrafish GATA-5_Human ENSPTRG00000013711_Chimpanzee ENSCAFG00000012709_Dog ENSMUSG00000015627_Mouse ENSRNOG00000036732_Rat ENSGALG00000005352_Chicken ENSTNIG00000002769_T_Puffer ENSDARG00000017821_Zebrafish GATA-6_Human ENSBTAG00000005734_Cow ENSMUSG00000005836_Mouse ENSRNOG00000023433_Rat ENSTRUG00000012674_J_Puffer ENSTNIG00000014837_T_Puffer ENSDARG00000035831_Zebrafish ENSXETG00000003144_Frog FBgn0003117_Fly

. : EMRPIKTEPGLSSHYGHSSSVSQT-FSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA-----------------------------------------------------------------EMRPIKTEPGLSSHYGHSSSVSQT-FSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA-----------------------------------------------------------------EMRPIKTEPGLSSHYGPSSSLSQT-FSVSAMSGHGSSIHPVLSALKLSPQGYASSVSQSPQASSKQDPWNSLALADSHGDIITA-----------------------------------------------------------------EMRPIKTEPGLSSHYGHSSSMSQT-FST--VSGHGSSIHPVLSALKLSPQGYPSPVTQTSQASSKQDSWNSLVLADSHGDIITA-----------------------------------------------------------------EMRPIKTEPGLSSHYGHSSSMSQT-FST--VSGHGPSIHPVLSALKLSPQGYASPVTQTSQASSKQDSWNSLVLADSHGDIITA-----------------------------------------------------------------EMRPIKTEPGLSSHYGHPSPISQA-FSVSAMSGHGSSIHPAISALKLSPQAYQSAISQSPQASSKQDSWNSLVLAENHGDIITA-----------------------------------------------------------------EMRPIKIEPGLSPPYGHSSSLSQA-SVLSTITSHGSSSYP-MSSLKLSPQNHHSTINTSPQASSKHDSWNNLVLA--------------------------------------------------------------------------EPRHIKTEPDTHSLYTHHSAHAQ----ISALPAYMT-AHGGAIPLKMSPGGHT------GSSGSKAEPWNSLILA--------------------------------------------------------------------------EPRHIKTEPESHSLYSHHSTHAQSPLQISALPAYMA-THSGAIPLKMSPGSHG------GSSGSKAEPWNNLILA--------------------------------------------------------------------------ETRPIKTEPDTTPLYTHHNIHTQ----VSAFPAYMG-SPS-------------------GSSSTKSEVWNSLILA--------------------------------------------------------------------------SLASPVCPGPSMA--PQASGQEDDSLAPGHLEFKFEPEDFAFPSTAPSPQAGL-------RGALRQEAWCALALA--------------------------------------------------------------------------SLASPVCPGPSMA--PQASGQEDDSLAPGHLEFKFEPEDFAFPSTAPSPQAGL-------RGALRQEAWCALALA--------------------------------------------------------------------------SLASPSCPGSSIT--SQAPGPVDDPLAPSHLEFKFEPEDFAFPSAALGPQAGL-------GGTLRQEAWCALALA--------------------------------------------------------------------------SLASPVCAGPTIT--SQASSPADESLASSHLEFKFEPEDFAFTSSSMSPQAGL-------SGVLRQETWCALALA--------------------------------------------------------------------------SLASPGCAGPTIT--SQASSPADESLASSHLEFKFEPEDFVFTSSSLSPQAGL-------SGVLRQEAWCALALA--------------------------------------------------------------------------STTS-QYPGQGIVSVSQAQSQSDEALAGG--EFKFEPEDYPFSPSSMAPQPGL-------SVPLRQDSWCALALA--------------------------------------------------------------------------SSAPSPYAGQTLAAATQTASHLD-RVG--QVDIKY--EDYPFAPASISSP----------------HSWCALSQA--------------------------------------------------------------------------SIAASPYAGQTVVSVTQASTQLD-SASSAHVDIKY--EDYTYTPTSIAPQ----------------NSWCALSQA--------------------------------------------------------------------------NTSPTTQPTASGAGAPVMTGAGE-STNPENSELKYSGQDGLYIGVSLASPAEV-------TSSVRPDSWCALALA--------------------------------------------------------------------------NTSPTTQPAASGAGGSVMSGAGD-SANPENSELKYSGQDGLYIGVSLASPAEV-------TSSVRQDSWCALALA--------------------------------------------------------------------------NTSPSTQATTSGVGASVMSAVGE-NANPENSDLKYSGQDGLYIGVSLSSPAEV-------TSSVRQDSWCALALA--------------------------------------------------------------------------NQSPPPQSTPYEVGASVMSAVGE-SANPENSDLKYSGQDGLYIGVSLSSPAEV-------TSSVRQDSWCALALA--------------------------------------------------------------------------TSSPPGQG--SGVSSSVLSSSGE-GTGSS-SAVKYPGQDGLYTSVGLSQASDV-------AS-VRGEPWCPMALA---------------------------------------------------------------------------SSPSGQG--SGVSSSVLSSSGE-GTGSS-SAVKYPGQDGLYSSVGLSQAADV-------AS-VRGEPWCPMALA--------------------------------------------------------------------------NSPSSTQVPVTSVNSSTLVGQVD-VPGTTGSMVKFPGQESLYTNVGLTSSADV-------ASSVRGDSWCPMALA--------------------------------------------------------------------------NGSPSQNT-APVVASSLMSTQTD-STSPNSNTLKYTGQDGLYSAVSLSSASEV-------AASVRQDSWCALALA--------------------------------------------------------------------------RHSLSKLHTDMKSGTSSSSTLMGHHSAQQQQQQQQQQQQQQQQQQQQSAHQQCFPLYGQTTTQQQHQQHGHSMTSSSGQAHLSARHLHGAAGTQLYTPGSSSGGGSASAYTSHSAETPALSNGTPSPHYQHHHHLGGTHGHHVTAAAAHH .......610.......620.......630.......640.......650.......660.......670.......680.......690.......700.......710.......720.......730.......740.......750

------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HFHAAAAVAAYGVKTEASATNYDYVNNCYFGGTFGALGGAATTTAMAGGAASELAGYHHQHNVIQAAKLMATS .......760.......770.......780.......790.......800.......810.......820...

442 399 442 441 442 411 394 386 386 352 397 397 400 404 404 391 392 383 595 499 589 587 511 497 501 524 540

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