Ausubel, R. Brent, R. Kingston, D. Moore, J. Seidman, J. Smith, and K. Struhl (Eds.), Cur- rent Protocols in Molecular Biology. John Wi- ley and Sons, New York.
Terminal restriction fragment (TRF) patterns obtained using the T-RFLP technique are generated and analyzed in a procedure that combines PCR with a labeled ...
We developed an efficient, cost-effective, and rapid purification method for .... The simplicity and cost-effectiveness ... Seo, T.S., Z. Li, H. Ruparel, and J. Ju. 2003 ...
Michael Sexton and Sebastian George Barton Furness. Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences,. Monash University, Parkville ...
core Jvm (e.g., monitor implementation, object al- location) and JIT ... that simulate the activities found in complex on-line transaction .... IBM DK for Windows NT, Java Technology Edition, ... tiers, client and server, ran on Windows NT with Ser-.
cluding the Java virtual machine (Jvm), core class libraries, the just-in-time (JIT) .... IBM DK for Windows NT, Java Technology Edition, version 1.1.7, the Jvm in ...
Performance measurement tools are very important, both for designers and users of data- ... It is specifically dedicated to OLTP applications, and features a complex data- ... (2011) propose benchmarks that are specifically tailored for testing.
ety of purposes, such as explaining or predicting system and application per- formance ... Correctness and Performance Testing Languageâ) is a programming ...
Colin Phillips. Department of Linguistics ... [email protected]. Abstract ..... dependency is a gap in an argument position in some models (Gibson and Hickok 1993;.
best of breed," in that it is superior to other similar algorithms 10, 9, 11, 4, 15 . ... stochastic local search such as GSAT or WSAT , or to algorithms designed speci ...
LLP; or the Centers for Medicare & Medicaid Services (CMS). ... Health Clinic. Significant differences .... Outpatient Medicare costs per dollar of outpatient. Medicare ..... North Carolina at Chapel Hill, 725 Martin Luther King Jr. Blvd.,. CB# 7590
Jul 14, 2008 - The profile (i.e., a vector indexed by the players) TV is called the true ..... In virtue of the revelation principle, our mechanism of course has a ...
ULTRIX V4.3 (Rev. MP-3.22). Compiler: MPL Version 3.2.14. Processors: proprietary RISC. Memory per Node: 64 Kbytes. Intel iPSC/860. Operating System:.
cation of signal intensity in numerous cell-sample lysates using a Bio-Rad. IQ5 spectrofluorometric detection system (Bio-Rad Laboratories, Madrid,. Spain).
Associate Editor: Trey Ideker. ABSTRACT. Motivation: In recent years, the biological literature has seen a significant increase of reported methods for identifying ...
Jun 5, 2007 - Peter M. Kyveryga,* Alfred M. Blackmer, and Thomas F. Morris. ABSTRACT ..... across all rates of N. The EXP and SR models always impose a bias so that ..... Anderson, R.L., and L.A. Nelson. 1975. ... 139â164. In R.F. Follett et al. (e
NASA Ames Research Center, is a large scale e ort to advance the state of computational .... All rules apply equally to subroutine calls, language extensions and.
aPoznan Supercomputing and Networking Center, Noskowskiego 10 Street, 61-704 ..... parts for given parameters set constitutes what we call the optimization.
These techniques are applied to the SPEC CPU95 and CPU2000 benchmark suites. It is shown that these suites contain both eccentric and fragile benchmarks.
simple and reliable enough to allow its application in ... cobacterial nucleic acids as template molecules for .... author at the EMail address(s) below. BioTechNet: ...
Address correspondence to Eric S. Ramsson, De- partment of Biomedical Sciences Grand Valley State. University, 208 Henry Hall, 1 Campus Drive, Allen-.
primers are used to amplify mouse. Kdm5c and Kdm5d from genomic DNA, generating amplicons differing by 29 bp. For the rat, we have identified a common.
control experiment, 1% Triton X-100 and. 10 mM CHAPS, without sarkosyl, did not affect GST enzymatic activity. GSH. Sepharose binding was also significantly.
fication of GC-rich DNA templates have been developed (8â9), but ..... with this free weekly electronic newsletter delivered every Thursday to BioTechniques.
Benchmarks
Benchmarks Enhanced amplification of GC-rich DNA with two organic reagents Zhizhou Zhang1,2, Xia Yang1,2, Liangyu Meng2, Fang Liu2, Cenchao Shen1,2, and Wenjuan Yang1,2 1HIT Bio-X Centre for Systems Biotechnology, Harbin Institute
of Technology, Weihai, China and 2Teda Bio-X Centre for Systems Biotechnology, Tianjin University of Science and Technology, Tianjin, China BioTechniques 47:775-779 (September 2009) doi 10.2144/000113203 Keywords: GC-rich DNA; ethylene glycol; 1,2-propanediol; PCR Supplementary material for this article is available at www.BioTechniques.com/article/113203.
Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents—ethylene glycol and 1,2-propanediol—were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60–80% and lengths of 700–800 bp. Researchers have put much effort into how to conveniently amplify GC-rich sequences using additives (1–6). Novel thermal cycling patterns such as ‘slowdown PCR’ can also work efficiently for some GC-rich DNA amplification (7). Mechanisms for overcoming difficulty in the PCR amplification of GC-rich DNA templates have been developed (8–9), but amplification of GC-rich DNA sequences is still not an easy task worldwide. Preliminary bioinformatics analysis from the Ensembl database (www.ensembl. org) indicated that the number of intragenic GC-rich DNA fragments (>65%) was 773. About half of these GC-rich sequences were
