Additional file 2 â Primer sets designed using BatchPrimer3 for 184

Additional file 2 – Primer sets designed using BatchPrimer3 for 184 nuclear microsatellite loci in Asclepias syriaca not tested for amplification success See Table 2 for 25 successfully amplified additional loci. Primer names with identical numbers followed by letters indicate multiple microsatellites originating from the same contig. Primer Set Name As121 As370 As525 As738 As810 As1220 As1274 As1380 As1678 As1925 As2003b As2159 As2819 As5408 As5755 As5757 As5836

Orientation Primer Sequence (5'-3') FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE

GTCGTCTTCCCCTTTTTC GTTCTATTCGAATTTCGTTCC CCTCACTTTAAGTTGGTGGA TTATCGAGTCGAATGAGATGT TGGAAGGAAGGTAGTAAGGTC AGATTCCCTTGAAGCTAGAGA AATGGCATAGAGGCATATTTA CTATGCTATCAAGAAGCAGGA TAGATGATAGCACCAATCCAC AGGACAAGATAAGATGGCCTA TTGGGAATCAACTGAACTAGA CCGGGAGAAAAGTGATCT GTAAAGTGGCATTGAGGAGAT AAACGTGACTGGGTCATCTA CTGTTGATTGTTTGGACAGAT AACTAAATGCACGGTAAGATG CACCCCTATACGGATAACTTC TCTTTCTGTATATGGCAAAACTT GACATCGATTTTGCACATATAC TACTCACCTCAACAAAATGGT AGCCCCTTTATTAAGTGATTG TAGGCGATTTAAGCTTTATGA AGCTTAGCTTGAGGGTTAAAG ACTATTGAAAGAAAGACAGGTG AAGCTCCTACACCTCCATATC TCTCTTATTTATGGAACTTCTTC AGTTAAGAAGAAGTGGGTTCG GAAATGCCAATTCAACTCTCT TCTCAGCTAGACTCAGAAGGA GAAAACTACCAAGACTGACGA TTGAAGCTACCCGTAGATATG AACCTCTTTAGTTTTGCCAAC GAATGTCGTCCTTTCATTGT CGAATAGGAAGGTATGGAAGT

Tm (°C)

Repeat Motif

Product Size (bp)

54.6 55.5 55.3 54.9 55.1 55.0 54.2 55.0 55.2 55.6 55.0 55.1 55.9 55.6 55.1 54.6 55.3 55.1 54.8 54.7 55.2 54.7 54.9 53.0 55.0 51.7 55.3 56.0 54.5 54.6 54.7 55.3 55.0 55.0

AAAG3

114

CT4

100

TA4

109

AGC4

113

GCTT3

104

CT4

105

AG4

100

AG4

116

AT4

104

ATCG3

120

AATC3

110

TA4

123

TC4

180

CT4

115

GTTAG3

102

AG4

114

AG4

114

As6143a As6143b As6929a As6929b As6942 As7703 As8174 As8578 As8868 As8870 As9296a As9296b As9296c As9296d As9299 As9388 As9478 As10870a As10870b As11441 As11689a As11689b

FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD

AAGAAGTGCAACCATATATCAA GTTGTAAATTTCCTGCATTTG AAGAAGTGCAACCATATATCAA GTTGTAAATTTCCTGCATTTG GAGTGTTTGAAGAGGGATTCT TTCTTACTACTTTCCCATAGCC CGGCTATGGGAAAGTAGTAAG TAGGGGGAAAATCGAGTATAG TGGAATAGGTGTCTTCAACTG TTGGTCGGTCTAAAGATAACA AGGCTAAGCTCTCAACCTAAC CTTCAAAGCAAAGAGGGTAA AGAATGCTTCTTGCTAACATC CCTTAGAAAGCACAGACTGC GCCCTGACGATATAAGTTTTT GTGGCTCCATACCTCAGAT AAAGAAGATGCTGACACAAAG GACTTAGCTGACTACCATTCG GCAGCTCCTTCTATGCTTATT CACCATAATCGATTTCACATT TAAGTGGATCGTTTCTTCAAA AGGCTTCTGCTAAAAAGAAAA GAAGCAACATTAAGGCCTATC AGGAAAGGTTGTTAGAAAACTG GTTCCCTCCACTTATTAGAGC GCTTCAACGTAAAGGATAAGG TATCTAAGCTCGGCTAGAAGC TAGAAAACTGGGCCTTACTCT GTAGCAAAGCGAATGAACA TGATTAGGAACGATACGAGAA TTTTGAGAAACTACCGTTGAG AATGCATACCTTTATGATGCT GGCATCAAAATTCTCTAAACA AGATCGAAGAAGACTGTGATG TAGATTTTTCCGAAGACTGAG TACCAATAGGATTCTCTGCAA ACGAAGAGCAGGAGGTTAGTA CTGACTCCAATACAAATACCG AGCAAATAATGCCAAAAATC CAAGTACCGGGTAGGTAAGAT CCCGAGGGAAATTAATAGATA ATGAATGACCCATAGTACGC TTTTGTATTTGCCAACCTAGA

54.2 55.0 54.2 55.0 55.0 54.6 55.9 55.2 55.3 55.0 54.8 54.9 54.0 55.4 55.2 54.9 54.8 54.3 55.6 55.0 55.1 55.4 55.7 54.5 54.8 55.8 55.9 55.0 55.0 55.1 54.8 54.1 54.7 54.5 54.0 55.1 55.9 54.9 54.6 55.0 55.1 55.0 55.2

TA4

122

AT4

122

GA4

121

AT4

121

TC4

111

TA4

108

GCAA3

112

TCTT3

103

AG4

101

GA4

122

TA4

100

AGA4

102

TCT4

119

GGATA3

106

AT4

103

AT4

108

CAT4

113

GA4

100

AAGA3

101

AAG4

100

TC4

121

AG4

107

As11689c As12120 As13014 As13124 As13403 As13641 As14767 As14821 As14892a As14892b As15779 As15913 As16072 As16238a As16238b As16392 As16526 As18901 As19495 As19903 As20020

REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE

ACGGGTGAAATCCTATATTTT GTTGCTTTGGACAGGTAAGAT GGGTAAGTTCCCAAGCAT GGCATTCCTTTATCTTCTAGC CACCAACTAACAGTCGGATAC CAAGAAGCGACTCAAACTCTA CCTATCCAGTCCAGAGAAAAT GCTTATGGTAAAGCTTCCTGT AAGAAAGAAAAGAGAGAGTTGC GGGAGAAATTCCCTATCTAAA ATCTGTGGTACTGAAGTCGAG GAAGTCAAGCAGTTCGACTAA GAAAACAAATTAGGAAAGAAGTC ACTAGTACCTCCCAGTGAAGC CACCCTTATCAAAACTTCCTT CCTTATTGCAGGAAAACACTA AACTACATTTATGCGAATACGG ACCAGATGAGATGATGTTGAG AGGGAACTCCACTAATTTATGA ACCAGATGAGATGATGTTGAG AGGGAACTCCACTAATTTATGA AGCCAAGCAAAATAAAAAGAG AATCGAGATTGGGTCAGTATT TTTTACCGAAGCAATACAAAG ACTTCATTTACGGCATTGATA GATTCCATCCAGAAGAATGAT GTATCTTTCTACCTCTCCTTGG TCATCTCAAATCAAACTACCG CACCATCCCTACCTTGATAGT AGTGGCGAAGTGTTGATTAC TTGATTTGAGATGAGAGGCTA CTATTGGCTTCACCAGTACC GCAGTGAGAAGGATATCATCA AAGAAGCACACCAGACTAGAA AGAGGCGAACATTTCTCTATT AAATAGCCAGATTGGGATAAG ACTGCTATTTCAACTGATGGA GTGTTGAACTGGATGACATTT GCGGTCTCACTTTTTCTAATA AATTAGGTCAAGGGATGCTAC CATTTGGGATATTTGAATCAC TCGTTTCAAGAAAGAGTTTTG GTAAGTTAGCGGCGTAATAAG

54.5 56.0 55.0 55.0 54.8 55.1 55.0 55.4 54.1 54.2 54.4 54.9 53.5 55.2 55.1 54.8 56.1 55.0 54.7 55.0 54.7 55.7 55.3 55.0 55.0 55.6 54.0 55.4 55.6 55.3 55.2 54.8 55.3 54.3 54.9 55.1 55.0 55.0 54.0 55.0 54.5 54.9 54.0

AG4

102

TTCT3

107

AG4

131

CT4

100

AG4

110

CT4

103

CT4

106

AT4

116

TTCT3

109

CAATT3

109

GA4

133

TGA4

118

AAG4

120

TC4

128

GAA4

120

CT4

106

GCAA3

108

CA4

110

AG4

104

GA4

113

TCTT3

122

As20679b As20758 As20767 As21133 As21149a As21149b As21649 As22120 As22167 As22376 As22687 As22763 As23491b As24305 As24519 As25019 As25105 As25487 As27644 As27669 As28083 As28858


AATGAAGGTTGAGTTGAATGA CGAGAAGAGATAAAAGCAGAA CCTTTAACCTTTAACCGAGAA GGAAGGACCAGAGAATTTAGA TCTTTCTGATGAGACTTTTGC AGTAGGAGCCTTTTCTACCAA CCGCAGTTTTTCTTGATTAT CCTCTTGGTCTACAGCCTTAT ATACATTCTCCGTGCCTTTA TGAACTAATTCCTTCTTGTGC GGCATATCTTTGTGCCTACTA GGAACTGATGACCTTTCTTCT AACCATCCTCCTTATGTCACT AGTGTAAGGACAAGAAGCACA CCACTCGACTGTAAGGATGTA GCACTGTCCCAAAGAGTAAT TGCTGTATGAATTTGAAGAGTC ACCAATTTGATAACAAGAGCA AAAGTGCGCTTATAAATCGTAT GGTGGTATTCTCAGGGATAAC CGATATTCAACGACTAACGAT CCTTCCATTTTACAGAGGTTT ATGATAGGTTTGAGCCGATT GTTGCTCCTTCTACCCTTAAC TCTCTGACTACCCACAAGATG GGAGTTTTGTGACATTGAAGA GGAGCCTGATGTACAACTTAG CATCTGCACTGTGATGAGTC CCAATAAACAAAGAGAAAGCA GCCTTACGTAGATGTAGTGGA AATCATTCATTTCCTCTATCGT CAAACAAATATGGCAATAAGG GCTTGCTTACTTCATTTTACG CTAGCCCATACCCTTTGAGT ACGCAACTACATATGTCCATC ACAATAGCGAGAAAGGAAAAC AGAGAGAGAGAGATGAGGAAGA TTTTCGTCCCTAAACTTTGTT CGAATCATATGTTGTCGATAG ACAGCGCTTACTCATCAAAG AAACATCAACGGTAGTGTCAT AGAGGAGAATGGTCAGACTTC AGGTCAGGGTATTGGTAGAAA

54.8 54.3 55.4 55.1 54.8 55.0 54.7 55.3 54.9 54.7 54.7 55.0 55.2 55.1 55.4 54.4 54.7 55.0 55.2 55.1 54.6 55.1 56.3 54.8 55.3 55.3 54.2 55.0 54.9 54.6 54.6 55.0 54.7 55.9 55.1 55.4 54.1 55.7 53.3 55.8 54.6 55.0 55.4

TTCG3

104

AT4

119

AGAA3

114

CT4

109

CT4

122

AG4

144

AC4

101

TG4

102

TA4

130

AT4

100

TAAC3

105

AC4

101

CTA4

103

GA4

137

AG4

112

TA4

100

ATTC3

104

CT4

135

AT4

106

AT4

105

CA4

107

CT4

111

As29134 As30342 As30511 As33389 As34183 As34695 As35600 As36198 As37690 As38257 As40666 As40723 As41059 As42098a As42098b As42675 As42784 As45012 As46803 As50236 As53582


ATCCATATCGACTTCCAAGAG TTGAGAACTGAACTGAGAAGC CCGTAGATGGATGAATCATAA AAGGATCTAACTTCAACTTTCG CTCTTTGCGTGGATAATCTAA GACCTGATCCCCTTATAGAGA GGTTCGATCTGACATCTTTACT AGGGGATAAGAGAACTGGATT GGGACTACCCAGCTACTTCT TTTAAAGTTTTAGTGGGCAAG CGTGCACTCCTTTTAGATTC ATGAAAGAGAAGAAAGAGTGAGA GGGTTTACAGTTTCCAAATTC CCCTCTTTTCCAACTAGAACT AGGAATTAGGCTTAGAAGACG TCGAAGAGCTAGATGTAATGG ATAGGTTGGAGTTCCTGTAGC GCTCTTCTAGCTTTGGATCTT GTATTTATCGAAAGACGAGCA GACCTTAACTCGGTCTTGACT TGTCTTGTGAAAAGAAACTCA TCTTCCATTGATCAACCATAC GCTGACGTCATGGATTATTT AAGTAATCGACGGAGTGTGTA ACGTTCGACACTTGGAACT GAACACAAACCAATGGTAGAG AGGTATTCCTGTCTCCTTCAG GAATCATGTCTGGATTTAGACTC CTCTGGTTGGCTTATATGGTA GCCACTCACTATCCACTATCA AAGCCGTAGTTCTAGTTAGGG CCTGAGGATCAAATTCCTTAT GGGTATAGCTCATAGCTCAAAT ATCGTCGATGATCGGTATAAT CCAGGCATTAAGCTATAGACA CTGGGACAACAATAAAATCAA CAGCGTGCTTTTATGAACTAT GGATCAGGTAAGGTAAACGTAA TCTCAATCTTTCAATTCATGG AGGAGGAAAGAAACATCCAT ATGGTGATTATGGACTCAGC ACACTCTTCATCAGCTTTTCA GTTAGAGAAGGTGGTTGCTCT

55.8 54.9 55.1 54.6 54.9 54.9 55.1 55.9 55.1 54.2 55.1 54.3 55.3 54.4 54.7 54.8 55.1 54.8 54.8 55.1 53.6 55.0 55.2 54.9 55.6 54.7 55.1 54.6 54.6 55.3 54.7 54.9 54.5 56.1 54.9 55.3 55.0 55.2 55.3 55.2 55.0 55.2 55.3

TC4

111

TA4

106

AG4

111

AG4

109

GT4

101

TC4

100

GA4

116

GA4

107

CCAATA3

170

CT4

100

GA4

130

AC4

100

AG4

159

TA4

122

CT4

101

TA4

100

AT4

103

TGAT3

109

TA4

133

CA4

116

TC4

108

As54423 As55542 As55715 As55919 As57343 As57505 As58725 As59892a As59892b As60548 As61119 As63797 As64039 As64726 As65701 As65986 As66599 As66787 As67765 As68506 As69254 As70080


GCTTTAATGGTTATGTGAAAGA AACAAGATGAAATTTAGGGTTC ACCCAATCAATTAACCCTAGA GTGAAAGAACTTAAGGCAATG GCAAGTTTTTGCTCTTGATT AAACCTGTACATGGAACAAAA ATCAATTCATGATGCGAAAT TTCCTTTTCTTGGAACAATTC CGTTAGCACTTTTGACTTGAC GACTGCTTATTCCGCTAAAAC TAAGAAGTGCCCTGCATACT AAGAAGCTTTGGCTTATTTTC TAGATAGACTTCTGCCAGCAC ATTCCGGTCTCACAAGAAA AGTGTACAGGCTACGGTACG ACATAGCCATAGCCGAATTA CAAGAAGAGACAACACTCTGG GTCAAGCTGCTCCTCTCTC CGTTCATCCTTAATTTTCTTC ATGATGAACAGGAAAGGAAAC ATCATGCTCAGAAAATAATGC TGCTACCATGTACTCTAGGTTT GAATAGATAACCCCTCAGCAC GTCTCATCCGTATCATTTCAA ACTTTCCATTGCAATCATCTA CTGAATGGATGATGAACTTTT TGATTAATTTGTTGTGGCTCT ACCGAACTACAGAGTCGTACA TGTAAACGAACAACTGTACATAA CCACCACTCTACTACCTTCGT TGCAGTAGCCTACAGTAGTGA GTCTCTAGTCGAGATGGGAAT GAGACTAAGAGGTGCCTGATT TGAACCAAACTATCTTACCTCA TATAGATGGCACGTATTACCC CATACGGTCAGAAAGCTCAG ATGATGTTGTGTTCGAAGAAG ACTCCTTGTTCTTCTCCACTC TGATTCATCATCCTCTTTGTT CCAATGGATATCACATGAAGT GAATGGGTATTTAGGCTCTGT AAATCCAGTTAAAACCACCAG CCTGCAACTATTCCTCTCTTT

53.9 54.3 55.3 54.3 54.8 54.8 55.5 56.0 55.3 55.9 55.2 54.8 54.9 55.6 55.5 55.1 55.1 55.2 53.4 55.7 54.6 53.5 55.4 55.2 54.9 54.3 55.0 55.0 52.9 55.9 53.9 55.0 55.2 54.2 54.4 56.1 55.3 55.1 54.7 54.9 55.0 55.8 55.4

AT4

118

ATGC3

107

TTTCA3

107

AT4

101

CT4

106

TA4

134

TA4

105

AG4

152

TACGG3

133

TTTC3

148

AT4

113

GA4

133

CATT3

111

TTTC3

107

AT4

108

AG4

114

AG4

144

GA4

113

AC4

121

AG4

100

TG4

107

TCT4

106

As71066 As72624 As74249 As74784 As77526 As80042 As82870 As86065 As88477 As91992 As92441 As93437 As95613 As97041 As97117 As97343 As98879 As99992 As102617 As109460 As110213


TTGTAACAAAAGGTGATCCTG GTTGACTCGCTTAAAAGAACA CCTAGGAAACATAACAAGGAC CATAATCCTCAAAGTCCAAGTT ATGTATGAAGTTGAGGTGCTT ATAACTTGTTGACTGCAAAGC GCACTTCAGCAGTAGATTCAA GTGAATGCATGGATTAAAGAA AATAGTGAAACCAAACGGAAG TTTCCTAATATCTGCTTGCTG GCACAGTAGGCTGTAGTCATT CTTGCATCATTTCATTATCCT ATAAATGTGCACTTGGTGCTA GGTTGGTTTATCATTTCTGCT TTGTCACATTGAATGAAGC TCTTCAATATAACCATCATTCG TAGATGCAGATAGGCAAAAAC GTTTGTTGAGGGAAATTATCA CAATGCAACAATTTCTCAATC AAAGTCTTGGAGTTGAACCA TCTCTAGGTCAAGCACTGAAG TAGGCAGAATACAAAGATGGA CTCAACACTATTCCAGTGCAT CTTTCAACCAAATAGCTTTCA ATAAACATGCTAACCCGAGA TTTCTTTTCCTCTAACCACAA GTTCTGCTCCTTTGAATGTAA TCAAAGAAACAAGAATTGCAC AGGTTAGTATGGCTTGTTTCC CCGGGTTCTCATCTTTTATC GCTAGAGCAAGTTTTGCTACC GATTCAATCCGTTTGATAACTT TAACGTTTCTTCATGCATAGC AGACATGCGAATGGATCTT TGCACACTCCAACCTAGAGTA TGAGCCAAAAAGAGTAAGAGT GTCTTCACAATCAAATCTTCG CAGTGAGGAGGCAGAAAAC GAGGAGCTTCAGTATCAAGG GGACTCTTGAATTTGGTCTTC TAAACACAATAACCCGAAGTG TTATCAAAAGCAACCTCAAAG GCTCTAATCGATCCAATATCC

55.3 55.0 53.1 55.1 54.0 54.8 55.8 55.3 56.0 55.0 54.7 54.4 56.0 55.9 52.7 54.5 54.8 54.3 55.8 54.8 54.9 55.1 55.3 54.9 54.9 54.3 54.7 55.6 55.1 56.2 56.2 55.0 55.7 55.6 56.0 53.6 54.9 55.9 54.1 55.4 55.4 54.9 55.4

CT4

102

AAC4

109

AG4

102

CCA4

102

AC4

128

TA4

100

ATT4

123

TA4

104

TTG4

100

AT4

120

AG4

103

CTTTT3

104

TA4

112

CAAA3

129

TA4

100

TA4

100

GA4

100

GT4

105

CT4

109

TC4

121

GAT4

109

As110592 As110792 As111193 As111807 As116994 As119055 As119087 As120404 As123452 As125868 As126609 As129320 As135378 As136405 As138896 As139005 As141357 As156857 As166543 As166729a As166729b As167143


AATGGACATGAATGTTTTGAG AAGCAGAGGTGACAGTGATAG GTTCTTTCTATGCCGATCTTT CAACCTAGAGACAAACCAGAA TCCTCCAAATTTCAATTCTT TGTGGAATCAGTTGTACCTTT GAGATACCTCGAACATCATCA TGACTCTGCTCTCTTTATTCG ATACACAAGCAGCAGAATCAT TGAAACCGACAACTATTTCAT AACACACACACAAACAAAACA CCTCTGTTATGTCAACACTCG TTTCTGTTTCACTATGGAGGA CACATACGTCATCATTCAAAA CAAACAATGAAAAAGTGTCGT CTTTGAAGGATCCGAGTGTAT AGGTAAGGATGAAAACGAAGT GGTCTTTCAATGCTTTAATCTT AACTTCCCCAAGACTAATGAA ATTGTGTTCAATTGATCTGCT AAAGCAGAGTATGACCATTGA AAAATACCGATCAATCCTCTC TCTCAATGACAACCTACTGGT TTTCTTCACTTCAGCTTGCTA AGTAGGAGTCATTTCCTGACC TATCTTTAATGGCATAGTTCG CATTGAGAGAATCTGAGTTCG CTCAGTTCTTTAGCTCGTTGA TTTCACAATCTCTTCCCATC CCCACTATTTATGATAGAGATAGAA CATGGGGATCCCTTTTCC TCCCCATCTTAATACCACTCT ATACTTCACCGTAGGCTTCA AGAGAAGGAGTAGCGGATGT AATCTCCAAATTAATCAGTGC GAAGAAGATTTTGTGAACGTG GCAGAAAAGGCTGTTTTATACT AGGCCTTATGGACTAAATACC GGAGTATCGGCCTTAACAGTA AACTCATCAAAAGAGGCATTT GAGTATCGGCCTTAACAGTAA AACTCATCAAAAGAGGCATTT TGGCAAATATTCCACTGTTAT

55.1 54.6 55.3 54.6 53.9 54.7 55.1 55.0 55.0 54.8 54.9 55.9 55.0 54.6 55.4 55.9 54.7 54.6 55.5 54.8 55.0 54.9 54.7 55.7 54.9 52.5 55.1 55.1 55.1 53.2 60.1 55.3 55.0 55.6 53.6 55.0 54.8 54.2 56.1 55.6 53.9 55.6 54.8

GA4

105

TG4

114

TCA4

116

AGA4

102

CAG4

101

GAA4

104

GAAG3

101

AG4

103

GAAG3

110

CAAC3

116

TG4

137

CT4

101

TA4

118

TTAA3

131

TTCC3

124

TTAT3

109

CT4

118

CCTT3

105

CTAT3

108

AT4

105

AG4

104

TC4

110

As171417 As173216 As175951 As184386 As188796 As195934 As208556 As213427 As213687 As228706 As250590 As272595 As279352 As309843 As316336

REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE FORWARD REVERSE

TTTTTATGGATGCTGAGAG TCTACCAACTTCACTTCATCTG GAAGAAAATCAAAGCTACAAGC CGTCGTGAGATGGAAGAA ACAAGGAGAATTGGATGAACT AGTCTTAAGAAACTGGGGAAG CGTTGGTCCACTCTCTTACTA CACAAATAGAGTCGAGGATTG CAAAAGGAAAAAGTAGACAGTG TGTAATTCCATGTTCTGAGGA CTTCAGACTACCAAAGTGGAA ACCCTTGAGTCACTTGGTTA ACTCATGCCTTGAACAACA AACGGATGTAATAATGGGATT ATTTAGAATGTGAGTGGTGAC TGACCCGATAGACACTTTTTA ATGGTGTTAATGACCATTGTG ATTTGATACCTCGGCTTTATC ATCATCTCACCCAACATAACT GGTTGTATTTTCTCATTGAA CCATTTACATTTCCTAGATTTACC TCACTTTCCCTAAATAAACGTAA ATAAACTATCCGGTCTCATCC TGTTGACAATCTGATCCACTT GAGGCTTGTCAGTTTGAAAT ATACGAAAACCTTGACATTAGG ACAATTGCAGATGTAATGGAC TCCAACTTTATTGTTTCTGGA GGGAACCCAACTTTGATACT CCTCTCGTTTGTTGAATGATA GTGCAGCAACACAAAGTTTA

51.3 54.6 55.3 55.5 55.3 54.4 54.6 55.0 53.6 55.7 54.6 54.7 55.0 55.1 51.6 55.0 55.7 55.1 53.5 50.2 55.3 55.1 54.8 55.6 54.5 55.2 55.1 55.0 55.6 55.4 55.0

CA4

101

GT4

115

TG4

100

TA4

113

GT4

140

TG4

102

AT4

102

TC4

121

CT4

100

TA4

107

TC4

104

AG4

101

AG4

100

TC4

115

AG4

144

Additional file 2 â Primer sets designed using BatchPrimer3 for 184

Additional file 2 â Primer sets designed using BatchPrimer3 for 184

Suggest Documents

Report of BatchPrimer3 Primer Design