GC and GC/MS Applications

Applications

Chemical/Industrial

GC and GC/MS Applications

GC and GC/MS Applications Chemical/Industrial Free Fatty Acids in Water 1. 2. 3. 4. 5.

6. 7. 8. 9.

Acetic Propionic Isobutyric Butyric Isovaleric

Free Organic Acids in Water

Valeric Iso-caproic Caproic Heptanoic

120°C 20°C/min

80°C

205°C 2 min 6.13°C/min

1. 2. 3. 4. 5.

Acetic acid Propionic Isobutyric n-Butyric Isovaleric

6. 7. 8. 9.

Valeric Isocaproic Caproic Heptanoic

265°C 2 min

7

1 min 5

9 5 2

1 0

2

Sample: Column: Carrier: Split Ratio: Injector Temp: Injection Size: Liner: Oven: Detector temp:

4

3

6

7 8

1

6. 7. 8. 9.

6

8

110°C

5

6

4

0

2

Sample: Carrier: Column:

4

Time (min)

6

8

10

0.06 to 0.13% each acid in water Helium, 42 cm/sec, 24 psi (120°C) 1.8 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (40:1), 1 l, inlet 250°C Temperature program listed above Pub No.: 5091-6687E FID 300°C

Injection: Oven: Detector:

Cis/Trans Cyclohexanoic Acids

4-Pentenoic acid trans-2-Methyl-2-butenoic acid trans-3-Pentenoic acid trans-2-Pentenoic acid 133°C

3 4

6

10

220°C 160°C

150°C 1 min

3°C/min

10°C/min

185°C 3°C/min

2°C/min

1 min 1 2

8

2

10 mM each acid in water 25 m x 0.32 mm x 0.5 m FFAP (Part No. 19091F-112) Helium @ 10.8 psi (42 cm/sec), constant flow 20/1 260°C 0.5 l Deactivated with glass wool plug Temperature program listed above 260°C

Isobutyric acid Butyric acid Valerolactone 2-Methyl butyric acid Valeric acid

9

10°C/min

4

Free Organic Acids/C4-C5 Isomers 1. 2. 3. 4. 5.

3

120°C 1 min

cis/trans m-Methyl cyclohexanoic acid

cis/trans p-t-Butyl cyclohexanoic acid

89

7

0

Carrier: Column: Injection: Oven: Detector:

400

5

10 Time (min)

15

2

20

Helium 42 cm/sec, 24 psi (120°C) 1.8 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (40:1), 1 l, inlet 250°C Temperature program listed above Pub No.: 5091-6687E FID 300°C

Sample: Carrier: Column: Injection: Oven: Detector:

4

6

8 Time (min)

10

12

14

0.5 to 1% in acetone Helium 46 cm/sec, 27.7 psi (150°C), 2 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (150:1), 1 l, inlet 250°C Temperature program listed above FID 270°C

Chemical/Industrial

Free Fatty Acids 1. 2. 3. 4.

Acrylates

5. 6. 7. 8.

C10 C12 C13 C14

9. 10. 11. 12.

C15 C16 C1 C18

C19 C20 C21 C22

265°C (10 min) 200°C 1 min

10°C/min

1. 2. 3. 4. 5.

Methyl methacrylate Ethyl methacrylate sec-Butyl methacrylate Allyl acrylate n-Butyl acrylate

6. 7. 8. 9.

Hexyl methacrylate Cyclohexyl methacrylate Hydroxypropyl acrylate Unknown

2 4 5 6

1

160°C 15°C/min

60°C 10°C/min

35°C 1 min

3 7

8

5

9 10 11

0

5

Sample Carrier: Column:

12

1

10 Time (min)

15

2 3

9

11 min


Hydrogen HP-FFAP (Cross-Linked PEG-TPA), 10 m x 0.53 mm x 1.0 m (Part No. 19095F-121) On-column, 1 l Temperature program listed above FID

Alcohols

Air Methanol (Solvent) Water 1-Butanol, 0.046%

5. 1-Pentanol, 0.022% 6. 1-Hexanol, 0.018% 7. 1-Heptanol, 0.014%

160°C

45°C

20°C/min

1. 2. 3. 4. 5.

1-Propanol iso-Butanol 3-Methyl-3-pentanol 1-Butanol 4-Methyl-2-pentanol

6. 7. 8. 9. 10.

1-Pentanol 2-Ethyl-1-Butanol 1-Hexanol Cyclohexanol 1-Heptanol 4

4

2

10°C/min

10 8

6

5

7

9

7

1

1 2

3

10 min


45°C 1 min

6

5

Carrier: Column:

150°C

Applications

Alcohols 1. 2. 3. 4.

8

4

20

0.07 to 0.14% in acetic acid and acetone Helium 42 cm/sec, 24 psi (120°C), 1.8 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (40:1), 1 l, inlet 250°C Temperature program listed above FID 300°C


7 6

0

Helium, 4 ml/min at 45°C HP-5 (5% Phenyl Methyl Silicone), 30 m x 0.53 mm x 2.65 m (Part No. 19095J-123) On-column, 2 l Temperature program listed above FID 350°C


2

4

3

6 Time (min)

8

10

12

Helium, 29 cm/sec, 3.0 psi (45°C) 4 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.53 mm x 1.0 m (Part No. 19095N-123) Split (25:1), 1 l, inlet 250°C Temperature program listed above FID 250°C

www.agilent.com/chem

401

Applications

Chemical/Industrial


Ethylene Glycol Mixture 1. 2. 3. 4. 5.

Volatile Alcohols

Diethylene glycol Triethylene glycol Polyethylene glycol 200 Polyethylene glycol 400 Polyethylene glycol 600

200°C 100°C

20°C/min

1. 2. 3. 4. 5.

Acetone Methanol isopropanol Ethanol n-Propanol

110°C 45°C 5

20°C/min

2.5 min

0.5 min 1

3

1 3 2

4 2

4 5

7 min


0

Helium, 25 cm/sec Ultra-1 (Cross-Linked Methyl Silicone),12 m x 0.2 mm x 0.33 m (Part No. 19091A-101) Split (100:1) inlet 250°C, 1 l Temperature program listed above FID

Glycols/Diols 1. 2. 3. 4. 5. 6.

Ethylene glycol 1,3-Butanediol Ethylene glycol phenyl ether 1,7-Hepatanediol 1,9-Nonanediol 1,10-Decanediol 2 1

1

Carrier: Column:

2

Time (min)

3

4

5

Helium, 46 cm/sec, 1.8 3.0 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 15 m x 0.25 mm x 0.5 m `(Part No. 19091N-231) Split (300:1), 0.5 l, inlet 250°C Temperature program listed above FID 275°C


Aldehydes 180°C 50°C 3 min

8°C/min

1. 2. 3. 4. 5.

Hexanal Nonanal Dodecanal Tetradecanal Hexadecanal

35°C 0 min

3 1

150°C 10 min 10°C/min

1 2

(A)

(B)

2 4

5 5

3

6

4

15 min

3 5 4

15 min

20 min. Carrier: Column: Injection: Oven: Detector:

402

Helium, pressure program: 10 psi to 25 psi (4.25 min) at 1 psi/min HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.53 mm x 0.88 m (Part No. 19095Z-023) On-column, 1 l, inlet program: 50°C (0 min) to 180°C (8.45 min) at 12°C/min Temperature program listed above FID 250°C

Carrier:

Helium, 7 psi at 35°C, (A) use constant flow mode, (B) use pressure program 7 psi to 18 psi (10 min) at 1 psi/min Column: HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.53 mm x 0.88 m (Part No. 19095Z-023) Injection: On-column, 1 l Oven: Temperature program listed above Detector: FID 250°C

Chemical/Industrial

Aldehydes and Acids 7. 8. 9. 10. 11. 12.

Butanal 2-Methyl butanal Pentanal Hexanal Heptanal Octanal

Acetic acid Decanal Propanoic acid so-Butyric acid Butyric acid so-Valeric acid

13. 14. 15. 16. 17.

Valeric acid Hexanoic acid Heptanoic acid Octanoic acid Decanoic acid 250°C

2 1

10

11 12

13

7

5

0

Carrier: Column:

3

8

6

5

17

14 15

3 4

16

10

15

Aromatic Amines 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

11. 12. 13. 14. 15. 16. 17. 18. 19.

14

Beta-phenylethylamine N-ethylaniline 2,4-dimethylaniline N,N-dimethylaniline o-phenylenediamine m-phenyleneadiamine 1-naphthylamine 150°C Dibenzylamine Tribenzylamine 70°C 10°C/min 1 min 18

7 8 13

2 1 2 4 Sample: Carrier: Column: Injection: Oven: Detector:

3 4

Helium, 21 cm/sec HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (Part No. 19095Z-121) Split 20:1, 1 l 80°C FID


1. 2. 3. 4. 5. 320°C 5 min 35°C/min

n-Octylamine n-Nonylamine n-Decylamine Benzylamine Dicyclohexylamine

counts

19

6. 7. 8. 9. 10.

2 3

3000

17

n-Heptadecane Trihexylamine n-octyldecane 2,6-Dimethylaniline 2,4-Dimethylaniline

1

5000 4000

4

5

2000

12 9 10

6

7

8

9

1000

6 11

Carrier: Column:

HP Basic Wax Column Amines

Piperidine 2-methyl piperidine Aniline Benzylamine Alpha-phenylethylamine N-methyl aniline N,N-dimethyl aniline p-toluidine o-toluidine m-toluidine

5

8 min

20

Helium, 40 cm/sec, 11.7 psi (60°C), 2.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (40:1), 0.5 l, inlet 250°C Temperature program listed above FID 275°C


2

1

10°C/min

60°C 1 min 9

1. n-Butylamine, 10% 2. n-Hexylamine, 89% 3. Cyclohexylamine, 1%

Applications

1. 2. 3. 4. 5. 6.

Amines

15

16

0

6 8 10 12 14 16 18 100-250 g/ml each Helium, 1.1 ml/min, constant flow HP-35MS, 30 m x 0.25 mm x 0.25 m (Part No. 19091G-633) Split (50:1), 1 ul, 275°C Temperature listed above MSD

2

Sample: Column: Injection: Oven: Detector:


4

6

8

10

12

14

16

10

18 min

0.5 mg/ml in Hexane HP-Basic Wax, 30 m x .32 mm x 0.25 m (Part No. 19091N-613) Split (ratio) 100:1, 250°C 120°C FID 250°C

403

Applications

Chemical/Industrial


Primary Amines 1. 2. 3. 4. 5.

Aromatic Amines

n-Butylamine n-Amylamine n-Hexylamine Cyclohexylamine n-Heptylamine

6. 7. 8. 9.

n-Octylamine n-Decylamine n-Dodecanylamine n-Tetradecanylamine

220°C 25°C/min

45°C 2 min

1. 2. 3. 4. 5. 6.

Xylene Aniline Dichlorotoluene p-Nitrochlorobenzene N-Methylformamide Formamide

7. 8. 9. 10. 11.

Phenazine 2-Nitrodiphenylamine 4-Nitrodiphenylamine 4,4'-Dinitrodiphenyl ether 4,4'-Dinitrophenlamine

4 7

6

50°C 2 min

8 9

3 4

5

1

250°C 5 min 10°C/min

1

3

6

2

2

9 5

1 1

7 8 10

2

4

6

8

10 min 35 min

Sample:

1 l split (ratio 30:1), 0.1 to 0.35% aliphatic amines in methanol Helium, 1.5 ml/min constant flow HP-35,15 m x 0.25 mm x 0.25 m (Part No. 19091G-131) Temperature program listed above FID 310°C

Carrier: Column: Oven: Detector:


Esters 1. 2. 3. 4. 5. 6. 7.

Ethyl Esters 8. 9. 10. 11. 12. 13.

Ethyl propionate Propyl acetate Ethyl butyrate Propyl propionate Propyl butyrate Ethyl valerate Butyl propionate

1

6 2 4 3 5


404

5

14. 15. 16. 17. 18. 19.

Propyl valerate Ethyl caproate Butyl valerate Propyl caproate Methyl decanoate Butyl caproate

Methyl dodecanoate Butyl heptanoate Methyl tetradecanoate Methyl hexadecanoate Methyl octadecanoate Methyl eicosenoate

1. 2. 3. 4. 5.

C4 C5 C6 C7 C8

6. 7. 8. 9.

C9 C10 C10 C12

200°C 5°C/min

45°C 1 min

200°C

8

5°C/min

45°C 1 min

9

7

0

Helium, 70 kPa HP-1 (Cross-Linked Methyl Silicone), 30 m x .53 mm x 2.65 m (Part No. 19095Z-123) On-column 1 l Temperature program listed above FID

11

4

15 13

10 12 14

2 16 17

10 15 Time (min)

18

20

19

3

5

6

9

7 8

1

0

25

Helium 29 cm/sec, 3.0 psi (45°C) 4 ml/min constant flow HP-INNOWax (Crossed-Linked PEG), 30 m x 0.53 mm x 1.0 m (Part No. 19095N-123) Split (25:1), 1 l, inlet 250°C Temperature program listed above FID 250°C


5

10 15 Time (min)

20

25


Chemical/Industrial

Phthalic Acid Esters 1. 2. 3. 4. 5.

Methyl Esters of Dibasic Acids

Dimethyl phthalate Diethyl phthalate Dibutyl phthalate Dicyclohexyl phthalate Dioctyl phthalate

200°C (2 min) 10°C/min

50°C 5 min

1. 2. 3. 4. 5.

6. 7. 8. 9.

C2 C3 C4 C5 C6

C7 C8 C9 C10

240°C (5 min)

8 9

6 1

2

3

5°C/min

45°C 1 min

5

45

7

4 1

2

3

30 min 0

15

20

25

30

35

Time (min)


Helium, 20 ml/min HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (Part No. 19095Z-121) On-column, 1 l Temperature program listed above FID

Fast Analysis of Laboratory Air



Chiral Compounds in Essential Oils and Gases 1. 2. 3. 4. 5. 6. 7.

1. Oxygen 2. Nitrogen

2

1,2-Dimethylbenzene Myrcene (-)-Camphene (+)-Camphene (+)--pinene 1S-(-)--pinene Cineole

pA

8. 9. 10. 11. 12. 13. 14.

(R)-(+)-Citronellal 1S, 2R,5S-(+)-Menthol 1R, 2S,5R-(-)-Menthol -Terpineol (+/-)-isoborneol (+)-borneol trans-Cinnamaldehyde 65°C 1 min 8

1

170°C

Applications

Carrier: Column:

5°C/min

14

100 2

80

1

5

7

10 13 9 11

6

60

12 34

40 0.00

0.20

0.40 Time (min)

0.60

20 5

Sample: Carrier: Column: Oven:

25 l, split (ratio 4:1) Helium, 87 ml/min HP-PLOT/MoleSieve, 15 m x 0.53 mm x 25 m (Part No. 19095P-MS5) 40°C

Sample: Carrier: Column: Injection: Oven: Detection:

10

Time (min)

15

20

0.25 ng/l each analyte in Hexane Hydrogen, 39 cm/sec, Constant pressure HP-Chiral , 30 m x 0.25 mm x 0.25 m (Part No. 19091G-B233) Split 30:1, 1 l, 250°C Temperature program listed above FID 300°C Pub No.: 5965-7873


405

Applications

Chemical/Industrial


Noble Gases 1. 2. 3. 4. 5. 6.

Inorganic Hydride Gases

Neon Argon Oxygen Nitrogen Krypton Xenon


35°C 3 min

4

1. 2. 3. 4.

5. Tetraborane 0.10 ppm 6. Pentaborane 0.10 ppm 7. Dihydropentaborane 0.60 ppm

Arsine 0.1% Phosphine 0.1% Selenide 0.1% Diborane 0.10 ppm

(A)

2 5

-70°C

1

(B)

4

30°C

6

1

2

3

5 6 2

GC: Sample: Carrier: Column: Oven:

4

Time (min)

6

5890 Series II GC EPC and TCD 250 l, split (ratio 50:1) Helium, 4 ml/min HP-PLOT/MoleSieve, 30 m x 0.53 mm x 50 m (Part No. 19095P-MSO) Temperature program listed above Pub No.: 5963-2466

Aromatic Compounds 1. 2. 3. 4. 5.

Benzene Toluene m-Xylene o-Xylene p-Cymene

3

8


7

Helium, 35 cm/sec HP-1 (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.5 m (Part No. 19091Z-205) Split (25:1), 1 l A: -70°C isothermal; B: 30°C isothermal FPD (535 m filter)

Chlorinated Isooctane 1. 2. 3. 4.

1 2 34

1-Chloro isooctane 4-Chloromethyl 2,2'-dimethyl pentane 3-Chloro isooctane 4-Chloro isooctane 4 2 3 Monochloro isomers

5

1

3

4

5 Time (min)

6

8 min Carrier: Column: Injection: Oven: Detector:

406

Hydrogen HP-FFAP (Cross-Linked PEG-TPA), 50 m x 0.32 mm x 0.52 m (Part No. 19091F-115) Split (150:1), 1 l 110°C FID


Helium, 33 cm/sec, 35.7 psi (80°C) 2 ml/min HP-INNOWax (Cross-Linked PEG), 60 m x 0.25 mm x 0.25 m (Part No. 19091N-136) Split (150:1), 0.5 l, inlet 250°C 80°C isothermal FID 300°C

7

Chemical/Industrial

Butadiene and Aromatics

1

Ketones 1. 2. 3. 4.

2

1,3-Butadiene Cyclopentene Benzene Toluene

1. 2. 3. 4.

3-Pentanone 4-Methyl-2-pentanone 4-Heptanone 5-Methyl-3-heptanone

5. 2,6-Dimethyl 4-heptanone 6. 2-Heptanone 7. 5-Methyl-2-hexanone

250°C

60°C 1 min

15°C/min

150°C 2 min

150°C

75°C 2°C/min

20°C/min

5 6

4

7

1 3 3

1

2

3

GC: Carrier: Column:

4

5 6 Time (min)

7

8

9

10 2

6890 Helium, 25 psig HP-PLOT Q, 30 m x 0.53 mm x 40 m (Part No. 19095P-Q04) Temperature program listed above FID, 275°C

Carrier: Column:

Oven: Detector: Butadiene Concentration: 200 ppm (v/v)

7. 8. 9. 10. 11.

2,4,6-Trinitrophenol 2,4-Dinitrophenol 4-Nitrophenol 2-Methyl-4,6-dinitrophenol Pentachlorophenol

1. 2. 3. 4. 5. 6. 7. 8. 9.

4 1 2

5 3

7

9

10

Time (min)

15

20

8

4,5

10 11

2 3

10

4-chloro-3-methylphenol 2,4,5-trichlorophenol 2,4,6-trichlorophenol Dinoseb 4-nitrophenol 2,4-dinitrophenol 2-methyl-4,6-dinitrophe nol 17. 2,3,4,6-tetrachlorophenol 18. Pentachlorophenol

25

20 g/ml phenols in methylene chloride HP 5MS (Part No. 19091-5433) 30 m x 0.25 mm x 0.25 m Helium, 33 cm/sec, constant flow 35°C (5 min), 8°C/min to 220 (2 ml) 1 l, Splitless, 250°C 0.75 min FID 300°C Pub No.: 5952-6971

3.0 Sample: Column: Inlet: Oven: Detector:


13

10

17 18

7 14,15

4.0


50°C 1 min

11 12

9

6

1

Sample: Column: Carrier: Oven: Injection: Purge Time: Detector:

8

10. 11. 12. 13. 14. 15. 16.

Phenol 2-chlorophenol o-cresol m-cresol p-cresol 2,4-dimethylphenol 2-nitrophenol 2,4-dichlorophenol 2,6-dichlorophenol

6

8

5

6 Time (min)

Phenols

Phenol 2-Chlorophenol 2-Nitrophenol 2,4-Dimethylphenol 2,4-Dichlorophenol 4-Chloro-3-methylphenol

0

4

Helium, 30 cm/sec, 8.8 psi (60°C) 21.75 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (50:1), 0.5 l, inlet 250°C Temperature program listed above FID 275°C


Phenols on HP-5MS 1. 2. 3. 4. 5. 6.

4

Applications

0

2

5.0

6.0 7.0 Time (min)

16

8.0

9.0

10.0

20 M/ml each HP-35MS, 30 m x 0.25 mm x 0.25 m (Part No. 19091G-633) 240°C, split 10:1, flow 1.1 ml/min Temperature program listed above MSD, 260°C


407

Applications

Chemical/Industrial


Free Phenols 1. 2. 3. 4. 5. 6.

Monomers in Latex Paint by Headspace

Phenol 2-chlorophenol 2,4-dimethylphenol 2-nitrophenol 2,4-dichlorophenol 4-chloro-3-methylphenol

7. 8. 9. 10. 11.

2,4,6-trichlorophenol 2,4-dinitrophenol 4-nitrophenol 2-methyl-4,6-dinitrophenol Pentachlorophenol

100°C 1 min 3

Acrylonitrile Methyl acrylate Butyl acrylate Styrene

40°C


3

10

5

4

2

9

4

200°C 30°C/min

90°C 15°C/min

5 min

6

1 2

1. 2. 3. 4.

1

7 8 11

GC: Carrier Gas: Column: Injector: Oven: Detector:

8

10 Time (min)

12

14

2


6890 Hydrogen, constant flow 45 cm/sec HP-50+, 30 m x 0.25 mm x 0.25m (Part No. 19091L-433) Split 100:1 Temperature program listed above FID 300°C

8. 9. 10. 11. 12. 13.

Propylene Propane 1-Butene Butene Pentane Toluene Styrene

C2H5-C(Ph) = CH2 C4H9-CH2CH2-Ph C4H9-C(Ph) = CH2 C4H9-CH=C(Ph)CH3 Styrene dimer 0°C Styrene trimer

280°C 60°C 5°C/min

3

1 4

0


408

8

5 10

20

1. 2. 3. 4. 5. 6.

1-Propene Butene Acetonitrile Acrylonitrile Propanenitrile 1,5-Hexadiene

12

30 Time (min)

7. 8. 9. 10. 11. 12.

1-Hexene Butylamine Cyclopentanone Cyclohexenone Hexylamine Caprolactam

1

13

10 2

8

12

10

Pyrolysates of Nylon 66

7

6

6

0.200 gm sample with 1 gram sodium sulfate in 5 ml water Helium, 45.5 cm/sec (40°C), 6 ml/min constant flow HP-INNOWax (Cross-Linked PEG) 30 m x 0.53 mm x 1.0 m (Part No.19095N-123) Split (10:1), 1 ml headspace, inlet temp 220°C Temperature program listed above FID 300°C


Pyrolysates of Polystyrene 1. 2. 3. 4. 5. 6. 7.

4

Time (min)

Abundance

6

6

5 50

100 mg pyrolyzed Helium, 30 psi, 12 ml/min HP-Ultra 1 (Cross-Linked Methyl Silicone), 50 m x 0.20 mm x 0.33 m (Part No. 19091A-105) Split (30:1), inlet 280°C, Pyrolyzer 600°C Temperature program listed above FID 300°C

60

Carrier: Column: Injection: Inlet: Oven: Detector:

A900°C Pyrolysis

9

10

40

15°C/min

0°C 1 min

2 34 5 7 8

9 11

275°C (2 min)

10

B700°C Pyrolysis 11 15 Time (min)

12 20

25

Helium, 5 psi HP-5 (5% Phenyl Methyl Silicone), 30 m x 0.53 mm x 2.65 m (Part No. 19095J-123) Direct interface to pyrolyzer (1000°C, 10 seconds) -10°C (0.5 min) to 300°C (1 min) at 120°C/min Temperature program listed above MSD 300°C

Chemical/Industrial

Pyrolysates of Kraton 1107 2-Methyl-1-propene 2-Methyl-1-butene 2-Methyl-1,3-butadiene 2-Methyl-2-butene 2,3-Dimethyl-2-butene 1,3,5-Hexadiene 3-Methyl-1,3-pentadiene Toluene Ethyl benzene

10. o-Xylene 11. Styrene 12. 1,4-Dimethyl-4-vinyl cyclohexene (ethyl methyl benezene co-elutes) 13. 1-Propenyl benzene 14. 1-Methyl-4-isopropenyl cyclohexene (dipentene) 275°C

3

0°C 1 min

TIC

10

4 1

5

2

2

4

Carrier: Column:

6 6

8

1

13 0

10 12 Time (min)

14

2

5

3

4

5

15°C/min

16

18

2

1

7

6 4

0

7

6 0.5

1

1.5

Time (min)

20 Carrier:

Helium, 27.5 psi (0.2 ml/min), 27 cm/sec Column: HP-Wax, 10 m x 100 m x 0.2 m (Part No. 19091X-241) Injection: 1000/1, 0.1 l Oven: 60°C (1.5 min), 30°C/min, 150°C (2 min)

Carrier:

Hydrogen, 33.7 psi (0.6 ml/min), 69 cm/sec Column: HP-Wax, 10 m x 100 m x 0.2 m (Part No. 19091X-241) Injection: 1000/1, 0.1 l Oven: 60°C (.56 min), 80°C/min, 150°C (0.1 min) Pub No.: 5965-6692

Solvents 1. 2. 3. 4. 5.

34

2 min

Helium, 5 psi HP-5 (Cross-Linked 5% Phenyl Methyl Silicone), 30 m x 0.53 mm x 2.65 m (Part No. 19095J-123) Direct interface to pyrolyzer (10 seconds) -10°C (0.5 min) to 300°C (1 min) at 120°C/min Temperature program listed above MSD 300°C

Injection: Inlet: Oven: Detector:

2

9 12

7

5. Toluene 6. C8 aromatics 7. 4-hydroxy-4methyl 2-pentanone

Propylene oxide Acetone Methanol Methylene chloride

14

11

8

1. 2. 3. 4.

Solvents

Methane Acetone Isopropanol Methylene chloride Methyl ethyl ketone

6. 7. 8. 9. 10.

1,1,1-Trichloroethane Isopropyl acetate Trichloroethylene Toluene n-Butyl acetate

11. 12. 13. 14.

150°C 10°C/min

40°C 3 min

1. 2. 3. 4. 5. 6. 7. 8. 9.

Perchloroethylene Xylene Xylene Xylene 180°C 20°C/min

Ethanol Acetonitrile Acetone Dichloromethane Diethyl ether Pentane Ethyl acetate Hexane Benzene

Applications

1. 2. 3. 4. 5. 6. 7. 8. 9.

Solvents in Paint Thinner

4 6 7 2

3

4

8

5

9 10

9

5 6

11 13

1

1

3 2

7

12

8

14 0

1


Helium, 24 cm/sec PONA (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.5 m (use 25 m only) (Part No. 19091S-001) Split (150:1); 0.1 l 250°C Temperature program listed above MSD 280°C Pub No.: 5953-8065

GC: Sample: Carrier: Column: Injection: Oven: Detector:

2

3 4 Time (min)

5

6

6890 1 l 0.1-0.3% solvents in methanol Hydrogen 9 ml/min HP-PLOT Q, 30 m x 0.53 mm x 40 m (Part No. 19095P-Q04) 250°C Split mode (12:1) 150°C Isothermal FID 250°C Pub No.: 5965-7872


409

Applications

Chemical/Industrial


Common Industrial Solvents 1. 2. 3. 4. 5. 6. 7.

MeOH Methyl formate EtOH Acetone i-PrOH CH2CI2 n-PrOH

8. 9. 10. 11. 12. 13.

Headspace Analysis of Common Solvents 1,4-Dioxane Toluene Mesityl oxide Diacetone-alcohol m-Xylene Cyclohexanone

14. 15. 16. 17. 18. 19.

MEK s-BuOH EtOAc i-BuOH i-PrOAc Nitropropane

140°C

10°C/min

1 2 45 3

58

19 2

17

14

8 6 7

1

11

16

9 15

Headspace Analysis of Common Solvents on Fast GC Residual Solvent Column

40°C 1.7 min

17. Benzene Hexane 18. Heptane Propanol 19. Trichloroethylene Methylethylketone 20. 1,4-Dioxane Ethylacetate Tetrahydrofuran 21. Pyridine s-Butanol 22. Toluene Chloroform Cyclohexane Vial Equilibration Time: 10 min Pressurization Time: 0.2 min 250°C Loop Fill Time: 0.15 min 90°C 30°C/min Loop Equilibration Time: 0.05 min 6°C/min 8 Injection Time: 1.00 min 45 19 10 11 6 15 7 23 1

1 GC: Column:

Carrier: Oven: Injection: Detector:

410

2

9. 10. 11. 12. 13. 14. 15. 16.

9

3

12

16 17 18

4 Time (min)

6890 HP-Fast GC Residual Solvent Column (Part No. 19095V-420) Helium, 35 cm/min, constant flow Temperature program listed above Headspace, 180°C, Split 7/1 FID, 260°C

GC: Column: Carrier: Oven: Injection: Detector:

5

12

16

19

18

22 21

20

21

6

8 Time (min)

6890 HP-624 30 m x 0.53 mm x 3.0 m (Part No. 19095V-423) Helium, 35 cm/min, constant flow 40°C (5 min) to 90°C at 2°C/min 90°C to 250°C at 30°C/min Headspace, 180°C, Split 7/1 FID, 260°C

12

14

Headspace Conditions Carrier Gas Pressure: Oven Temp: Loop Temp: Transfer Line Temp: Vial Pressure:

40°C 5 min

1. 2. 3. 4. 5.

30°C/min

90°C 2°C/min

3.5 psi 85°C 95°C 110°C 10 psi

Methanol Chloride (25 ppm) Chloroform (12 ppm) Benzene (25 ppm) Trichloroethylene (25 ppm) 1, 4-Dioxane (25 ppm) 3

1 4

2 2

4

6 Time (min)

HP-624, 30 m x 0.53 mm x 3.0 m (Part No. 19095V-423) Carrier: Helium, 35 cm/min, constant flow Oven: Temperature program listed above Injection: Headspace, 180°C, Split 7/1 Detector: FID, 260°C 3.5 psi 85°C 95°C 110°C 10 psi

10

250°C

Column:

6


4

Headspace Analysis of USP 467 Solvents

22 20

9 10

15

2

Helium, 35 kPa HP-1 (Cross-Linked Methyl Silicone), 25 m x 0.32 mm x 1.0 l Part No. 19091Z-212) Split (200:1), 1 l Temperature program listed above IRD 200°C Pub No.: 5954-0657

Methanol Ethanol Ether Acetone i-Propanol Acetonitrile Methylene Chloride t-Butanol

6 7

18

12 min

1. 2. 3. 4. 5. 6. 7. 8.

9. Benzene Hexane 10. Heptane Propanol 11. Trichloroethylen Methylethylketone 12. 1,4-Dioxane Ethylacetate s-Butanol 13. Pyridine Tetrahydrofura 14. Toluene Chloroform Cyclohexane Vial Equilibration Time: 10 min Pressurization Time: 0.2 min Loop Fill Time: 0.15 min 13,14 Loop Equilibration Time: 0.05 min 17 Injection Time: 1.00 min 11

20. 21. 22. 23. 24. 25. 26. 27.

4

10 13


Methanol Ethanol Ether Acetone i-Propanol Acetonitrile Methylene Chloride t-Butanol 3

12

30°C

Carrier: Column:

1. 2. 3. 4. 5. 6. 7. 8.

8

10


Vial Equilibration Time: Pressurization Time: Loop Fill Time: Loop Equilibration Time: Injection Time:

10 min 0.2 min 0.15 min 0.05 min 1.00 min

5

3.5 psi 85°C 95°C 110°C 10 psi

Chemical/Industrial

Fast GC Analysis of USP Solvents 1. 2. 3. 4. 5.

Methanol Chloride (25 ppm) Chloroform (12 ppm) Benzene (25 ppm) Trichloroethylene (25 ppm) 1, 4-Dioxane (25 ppm)

Solvent Paint Thinner 250°C 30°C/min

90°C 40°C 1.7 min

6°C/min 3

1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11. 12.

Methanol Ethanol Ethyl ether Acetone Isopropanol Cyclohexane

Methyl acetate n-Propanol MEK Hexane Isobutanol Benzene

1 23 56 8 9 4

n-Butanol MIBK n-Butyl acetate Ethyl benzene m, p-Xylene o-Xylene 100°C 5°C/min

250°C 15°C/min

50°C 2°C/min

35°C (2 min)

1

13. 14. 15. 16. 17. 18.

11 12 13

14

15

16 17 18

10 7

4

5

2

2 4 Time (min) 6890 HP-Fast GC Residual Solvent Column (Part No. 19095V-420) Helium, 30 cm/min, constant flow Temperature program listed above Headspace, 180°C, Split 7/1 FID, 260°C

Carrier: Oven: Injection: Detector:

6 Carrier: Column:

Rapid Analysis of Water in Organic Solvents

Ethylene oxide Ethyl formate Ethyl alcohol Water 2-Ethoxyethanol 2-Ethoxyethyl acetate

7. 8. 9. 10. 11.

Hydroxy acetate Acetic acid Formic acid Ethylene glycol/monoformate Ethylene glycol/monoacetate

87 ppm Water in Aniline

210°C 10°C/min

100°C

5°C/min

60°C 1 min

0 TIC (MSD)

5 2 1

0


Helium, 25 psi HP-1 (Cross-Linked Methyl Silicone), 50 m x 0.32 mm x 1.0 m (Part No. 19091Z-215) Split, 1 l Temperature program listed above FID


Ethoxyethanol 1. 2. 3. 4. 5. 6.

12 min

Applications

GC: Column:

6

10

0.2

0.4

11

0.6

0.8

1

1.2

Time (min)

4 3

78 9 5

10 Time (min)

Column:

15

20

Helium, 10 ml/min HP-FFAP (Crossed-Linked Polyethylene Glycol), 30 m x 0.53 mm x 1.0 m (Part No. 19095F-123) Split (10:1) Temperature program listed above MSD (280°C)

Carrier: Oven: Inlet: Inlet temperature Injection volume: Detector:

Pub No.: 5054-8215


HP-INNOWax 530 m x 15 m 1.0 m (Part No. 19095N-121) 15 mL/min helium, constant flow mode 100°C (1.5 min), post temp: 175°C (2.5 min) Purge packed; 240°C 0.5 l Thermal conductivity (TCD), 240°C

Pub No.: 5966-4131

411

Applications

Chemical/Industrial


Solvents 1. 2. 3. 4. 5. 6. 7. 8.

Solvent Purity Chloroform 9. 10. 11. 12. 13. 14. 15.

Isopropanol Unknown Methyl ethyl ketone Ethyl acetate n-Butyl alcohol Ethyl cellosolve Methyl isobutyl ketone Toluene

n-Butyl acetate Diacetone alcohol p-Xylene Cellosolve acetate o-Xylene Butyl cellosolve Butyl cellosolve acetate

1. Chloroform 2. Tetrachloromethane

100°C (2 min) 20°C/min

30°C 12 min

200°C (2 min) 5°C/min

70°C 3

1 2

45 6

12 13 14

10 7 8 9

11

15

1

17 min Carrier: Column:


Helium, 30 psi HP-1 (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.52 m (Part No. 19091Z-205) Split, 1 l Temperature program listed above TCD

Purity of Ethylbenzene

Styrene

ASTM Method D5060 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

60°C 1 min

4°C/min

290°C 6°C/min 120°C

4 1 6

6

12 1 0


1. Styrene 2. Diphenyl cyclobutane 3. Triphenyl hexanes

4

1


220°C 20°C/min

92°C (4.5 min)

Hydrocarbon Benzene Toluene Ethylbenzene p-Xylene m-Xylene Cumene o-Xylene Propylbenzene Styrene


Helium, 4.4 ml/sec HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.53 mm x 2.65 m (Part No. 19095Z-123) Split (50:1), 1 l Temperature program listed above TCD

3 2

5 5

3

10 Time (min)

7 8

9 10

10

11 Time (min)

31 min

15

Neat, 99%+ Helium, 32 cm/sec, 19.9 psi (60°C), 2.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 60 m x 0.32 mm x 0.5 m (Part No. 19091N-216) Split (100:1), 0.5 l, inlet 220°C Temperature program listed above FID 270°C


Hydrogen, 55 cm/sec HP-101 (Methyl Silicone Fluid), 50 m x 0.32 mm x 0.3 m (Part No. 19091Y-015) On-column, 1.5 l Temperature program listed above FID Pub No.: 5953-1540

412

BioPharmaceutical

BioPharmaceutical Purity of p-Xylene: ASTM Method D3798 1. 2. 3. 4. 5. 6.

Hydrocarbon Toluene Ethylbenzene p-xylene m-Xylene o-xylene

Styrene 220°C 20°C/min

92°C (4.5 min) 60°C 1 min

4°C/min 4

1. 2. 3. 4. 5.

Vinyl acetate Ethyl benzene o-Xylene Styrene a-Methyl styrene 3

1

5

100°C (2 min) 85°C 1 min

4

5°C/min

4 5

5 3

6

2


5

10

10 Time (min)

11 12 Time (min)

15

Neat, 99%+ Helium, 32 cm/sec, 19.9 psi (60°C), 2.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 60 m x 0.32 mm x 0.5 m (Part No. 19091N-216) Split (100:1), 0.5 l, inlet 220°C Temperature program listed above FID 270°C


Pub No.: 5953-1543

Analysis of Ethylene Oxide in a Pharmaceutical Formulation 200°C 25°C/min

75°C 50°C

5°C/min


3 min Hydrogen HP-FFAP (Cross-Linked PEG-TPA), 10 m x 0.53 mm x 1.0 m (Part No. 19095F-121) On-column, 1 l Temperature program listed above FID

Underivatized Drugs 1. 2. 3. 4. 5. 6. 7.

Ethosuximide Methylpropylsuccinimide Barbital Amobarbital Pentobarbital Secobarbital Mephobarbital

8. 9. 10. 11. 12. 13. 14.

Phenobarbital Procaine Methadone Amitriptyline Cocaine mipramine Nortriptyline

15. 16. 17. 18. 19. 20. 21.

Carbamazepine Desipramine Primidone Phenytoin Codeine Phenytoin Quinine

acetonitrile

280°C 80°C

20°C/min

ethylene oxide 1 2

8

3 2

3

4

5 Time (min)

4

5 7 6

13 11 12 9 10

6

14 15 16 18 17 19 20 21

Inlet: Column:

Split/10:1, 1 l, 250°C HP-WAX, 60 m x 0.53 mm i.d. x 1 m (Part Number 19095X-126) Carrier Gas: Hydrogen Head Pressure: 25 kPa at 50°C constant flow mode Flow: 5.2 ml/min Oven Temperature: Temperature program listed above GC System: FID, 250°C Pub No.: 5968-0322E


Hydrogen, 50 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.52 m (Part No. 19091B-112) On-column, 5 ng/component, 1 l Temperature program listed above FID


413

Applications

1

0

2

3

Applications

BioPharmaceutical


Underivatized Drugs 1. 2. 3. 4. 5. 6. 7. 8.

Opiates

9. 10. 11. 12. 13. 14. 15.

Ethosuximide MPS Barbital Probarbital Aprobarbital Talbutal Meprobamate Pentobarbital

16. 17. 18. 19. 20. 21. 22.

Methapyrilene Phenobarbital Amitriptyline Imipramine Nortriptyline Primidone Desipramine

Carbamazepine Primidone/phenytoin Diazepam Desmethyl diazepam Phenytoin Flurazepam Librium

1. 2. 3. 4. 5.

20°C/min

45°C 1 min

1 2

6

8 34

2°C/min

5

1

14 17 13 15 16 12 11

10

3 4

1819 20

9

50°C/min

200°C 1 min

2

7

5

21

1

22

1.5

GC: Carrier: Column: Injection: Oven: Detector:

5 min Helium, 30 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.17 m (Part No. 19091B-012) Split, 1 l, 10 ng/component Temperature program listed above FID


300°C

210°C 200°C (5 min)

Carrier: Column:

Meperidine Methadone Codeine Ethyl morphine Morphine

2 Time (min)

2.5

6890 Helium, 0.5 ml/min constant flow HP-1, 10 m x 100 m x 0.1 m (Part No. 19091X-141) 1 l sample, split (10:1) Temperature program listed above MSD

Pub No.: 5965-6692

Drugs of Abuse GC: Carrier: Column:

6890 Helium, 0.5 ml/min constant flow HP-1, 10 m x 100 m x 0.1 m, (Part No. 19091X-141) Split (10:1), 1 l sample Temperature program listed above FID (325°C)

Injection: Oven: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Caffeine Glutethimide Lidocaine Phenobarbital EDDP Methaqualone Methadone Cocaine Desipramine Carbamazepine

300°C 50°C

50°C/min

1 min

6

9 7 10

3 2 5

4 1

8

1

1.5

2

2.5

3

3.5

Time (min) Pub No.: 5965-6692

414

BioPharmaceutical

Underivatized Drugs 1. 2. 3. 4. 5.

6. 7. 8. 9. 10.

Barbital Pentobarbital Diphenhydramine Phenobarbital Methazualone

Codeine Morphine Quinine Haloperidol Strychnine

1. Phenobarbital, 100 ng 2. Primidone, 200 ng 300°C 160°C (5 min)

Carrier: Column:

3 2

Helium, 0.8 ml/min HP-1 (Cross-Linked Methyl Silicone), 12 m x 0.2 mm x 0.33 m (Part No. 19091-60312) Splitless, 1 l, 50 ng/ml Temperature program listed above MSD


1

2

1

40°C/min

50°C 2 min

5

8°C/min

6 10 4

3 min

9 7

Carrier: Column:

8 Injection: Oven: Detector:

24 min

Helium, 30 cm/sec HP-1 (Cross Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (use 5 m only) (Part No. 19095Z-121) Splitless, 1 l 220°C FID Pub No.: 5953-8066

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Barbiturate and Acidic Drugs

Methabarbital Barbital Allobarbital Aprobarbital Butabital Amobarbital Pentobarbital Methohexital Secobarbital Hexobarbital Phenobarbital Mephobarbital

40°C/min

150°C 1 min

8

4 3

1

1. 2. 3. 4. 5. 6.

280°C

10

7. 8. 9. 10. 11.

Ethosuximide Barbital Methyprylon Aprobarbital Butalbital Amobarbital

100°C

11

6

11

1

9

Phenobarbital Methaqualone Primidone Carbamazepine Dipenyhydanation 280°C 8 min 10°C/min

1 min

7

5

12. 13. 14. 15. 16.

Pentobarbital Secobarbital Meprobamate Carisoprodal Glutethimide

13

3

12

4

2

57 6 8

12 15

2

14

10 9

1 GC: Carrier: Column: Injection: Oven: Detector:

1.5

2 Time (min)

6890 Helium, 0.6 ml/min constant flow HP-1, 10 m x 100 m x 0.1 m, (Part No. 19091X-141) Split (10:1), 1 l sample Temperature program listed above FID (300°C)

2.5

Applications

Barbiturates

3

Pub No.: 5965-6692

0

Column: Carrier: Oven: Injection: Detector:

5

10

Time (min)

15

HP-35, 30 m x 0.53 mm x 1.0 m Helium, 40 cm/sec, constant flow Temperature program listed above Splitless, 1 l, 62 g/ml per compound FID (300C)


20

16

25

Pub No.: 5965-1622

415

Applications

BioPharmaceutical


Urine Drug Screen 1. 2. 3. 4. 5. 6. 7.

Amphetamine Methamphetamine Meperidine Phencyclidine (PCP) Methadone Proposyphene Amitriptyline 12

Serum Drug Screen 8. 9. 10. 11. 12. 13.

3

Cocaine Imipramine Cyheptamide (ISTD) Codeine Diazepam Flurazepam 45°C 1.5 min 4

300°C

1. 2. 3. 4. 5.

Ethchlorvynol Methprylon Butalbital Amobarbital Pentobarbital

6. 7. 8. 9. 10.

11. 12. 13. 14.

Secobarbital Glutethimide Phenobarbital Methaqualone Amitriptyline

Imipramine Cyheptamide (ISTD) Phenytoin Diazepam 300°C (2 min)

6°C/min

5

1

2

45 6 7

8 9

3

6°C/min

45°C 1.5 min 12

8 10 6

13 7

13

9

11

14 10 11

12

22 min


Carrier: Column:

Hydrogen 80 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 50 m x 0.32 mm x 0.52 m (Part No. 19091B-115) Splitless, 1 l Temperature program listed above FID


Alkaloids/Barbiturates 1. 2. 3. 4. 5. 6.

Barbital NC16 Amobarbital Pentobarbital Secobarbital Phenobarbital

7. 8. 9. 10. 11.

Hydrogen, 80 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 50 m x 0.32 mm x 0.52 m (Part No. 19091B-115) Splitless, 1 l Temperature program listed above FID

Alkaloid Street Drugs

Procaine Methadone Cocaine Codeine Heroin

280°C 14°C/min

50°C 1.5 min

1. 2. 3. 4. 5. 6. 7.

Procaine Methadone Cocaine Codeine Morphine Heroin Quinine

280°C 15°C/min 170°C 1 7 2

5

3 2

1

4

8 3

4

5

6

9

10

6 11

7

8 min 13 min Carrier: Column: Injection: Oven: Detector:

416

Hydrogen, 165 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.17 m (Part No. 19091B-012) Splitless, 1 l Temperature program listed above FID Pub No.: 5963-1708


Helium, 20 ml/min HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (Part No. 19095Z-121) Direct Injection,1 l, 12 ng/component, (packed column injection port) Temperature program listed above NPD

BioPharmaceutical

Drug Standard Mixture 1. 2. 3. 4. 5. 6.

Drugs in Urine

Caffeine Methadone Cocaine Codeine Monoacetyl morphine Diacetylmorphine

280°C (2.4 min) 50°C/min

50°C

1 2

1. 2. 3. 4. 5.

Amobarbital Phencyclidine Glutethimide Imipramine (IS) Codeine

260°C (5 min) 160°C 1 min

25°C/min

3 3 6

2 4

4 5 1 5.0 Time ( min)

6.0

5

7.0

0 Carrier: Column: Injection: Oven: Detector:

Helium, 0.8 ml/min HP-1 (Cross-Linked Methyl Silicone), 12 m x 0.2 mm x 0.33 m (Part No. 19091-60312) Splitless, 1 l, inlet 250°C Temperature program listed above MSD Pub No.: 5956-4809

Drug Standard 1. 2. 3. 4.

d-Amitriptyline d-Imipramine Cyheptamide d-Valium 130°C (.5 min)


2

4 Time (min)

6

8

Helium 65 cm/sec, 8.5 psi, constant flow HP-50+ (Cross-Linked 50% Phenyl Methyl Silicone), 30 m x 0.53 mm x 1.0 m (Part No. 19095L-023) Splitless, 1 l, inlet 275°C Temperature program listed above FID 300°C

Drugs in Urine 275°C (5 min) 230°C (.5 min)

18°C/min

16°C/min

180°C (.5 min) 25°C/min

30°C/min

1. 2. 3. 4. 5. 6. 7.

Nicotine PCH-(1-phenylcyclohexene) Phenmetrazine Cocaine metabolite I Phendimetrazine Nicotine metabolite I

8. 9. 10. 11. 12. 13.

Nicotine metabolite II Caffeine Phenobarbital Cocaine Homocaine Phenytoin

290°C (5 min)

260°C (5 min)

55°C (1 min)

5°C/min

13°C/min 110°C 3

3 4 6

1

2

2 5


11

8

4 7

9

10

12 13

17 min

Helium flow adjusted to maintain Cyheptamide RT = 15.00 min Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.52 m (Part No. 19091B-112) Splitless, 1 l Temperature program listed above FID Pub No.: 5956-4809


Helium, 28 cm/sec Ultra 1 (Cross-Linked Methyl Silicone), 25 m x 0.32 mm x 0.52 m (Part No. 19091A-112) Splitless, 1 l Temperature program listed above MSD


417

Applications

4.0

Applications

BioPharmaceutical


Barbiturates and Antidepressants 1. 2. 3. 4. 5.

6. 7. 8. 9. 10.

Barbital Amobarbital Phenobarbital Secobarbital Meprobamate

Phenobarbital Amitriptyline Imipramine cis-Doxepin Nortriptyline

Tricyclic Antipsychotics 11. 12. 13. 14. 15.

trans-Doxepin Despiramine Protiptyline Desmethyl doxepin Clomipramine 230°C (10 min)

23 5 4

9 6 10 11

Triflupromazine Promethazine Promazine Chlorpothixene Thioridazine

290°C (10 min) 250°C

1

10°C/min

10°C/min

3 min

4

3

10°C/min

225°C (6.5 min) 115°C 1 min

1

1. 2. 3. 4. 5.

2

5

7 8 12 13 14 15

12 min


10 min

Hydrogen, 80 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.17 m (Part No. 19091B-012) Splitless, 1 l, 15 ng/component Temperature program listed above FID


Antipsychotics 1. 2. 3. 4.

Hydrogen, 106 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 12 m x 0.2 mm x 0.33 m (Part No. 19091B-101) Split (75:1), 1 l Temperature program listed above Pub No.: 5954-7614 FPD

Antiepileptic Drugs

Promethazine Promazine Chlorpromazine Trifluoroperazine

290°C

2 250°C 3 min

20°C/min

1. 2. 3. 4.

Ethotoin PEMA n-Des Phenobarbital 1

1

5. 6. 7. 8.

2

Carbepoxide 10/11 Primidone Phenyltoin 100°C ISTD

3 4

6

14 min

5 min


418

8 7

3

Hydrogen, 106 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 12 m x 0.2 mm x 0.3 m (Part No. 19091B-101) Splitless, 1 l Temperature program listed above NPD

15°C/min

4

5

Carrier: Column:

230°C


Helium, 14 psi Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 12 m x 0.32 mm x 0.17 m (Part No. 19091B-012) Split (35:1), 1 l Temperature program listed above NPD

BioPharmaceutical

Antiepileptic Drugs in Plasma 1. 2. 3. 4.

Valproic acid Ethosuximide Phenobarbital Primidone

Anticonvulsants

5. Carbamazepine 6. Phenytoin 7. Clonazepam

200°C (16 min) 10°C/min

1. 2. 3. 4.

5. 6. 7. 8.

Ethosuximide Methylpropylsuccinimide Mephobarbital Phenobarbital

Carbamazepine Primidone Phenytoin Methylphenytoin

260°C

100°C 10°C/min

75°C

1

12 3

2

7

5

3 4

6


8

20 min

Nitrogen at 4 ml/min HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (Part No. 19095Z-121) Splitless, 1 l, 0.1 mg/ml Temperature program listed above FID Pub No.: 5954-9183

Antiepileptic Drugs in Plasma 1. Caproic acid 2. Valproic acid 3. Ethosuximide

7


Hydrogen, 50 cm/sec HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (Part No. 19095Z-121) On column, 1 l Temperature program listed above Pub No.: 5956-4809 FID

Amphetamines Injected as Salts 140°C 2 min 80°C 2 min

1. Amphetamine sulfate 2. Methamphetamine HCl

10°C/min 2

Applications

4

6 5

1

2 1


3

5 10 Time (min)

1.5 min

Helium, 20 ml/min HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (Part No. 19095Z-121) Splitless, 1 l Temperature program listed above FID Pub No.: 5954-9183


Helium, 30 ml/min HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 m (Part No. 19095F-121) Split (30:1), headspace 50°C isothermal FID


419

Applications

BioPharmaceutical


Cholesterol

Blood Alcohols 4. Palmitic acid 5. Stearic acid 6. Cholesterol

1. C6 2. C8 3. C10

300°C (5 min) 200°C (1 min) 160°C 1 min

10°C/min

20°C/min

1. 2. 3. 4.

5. 6. 7. 8.

Hold up time to Methanol Acetaldehyde Ethanol

Acetone Iso-Propanol N-Propanol (ISTD) N-Butanol

165°C 0.5 min 15°C/min

120°C 1 min

1 2 3 5 6 4

7 4

5

2

8

6

3

1

20 min

0

Column: Carrier: Column: Injection: Oven: Detector:

Helium, 25 psi Ultra 1 (Cross-Linked Methyl Silicone), 25 m x 0.2 mm x 0.33 m (Part No. 19091A-102) Split, 1 l Temperature program listed above FID

Oven: Inlet: Detector:

Flumethasone

1

0.5

1.5 2 Time (min)

2.5

HP-Blood Alcohol Column 7.5 m x 0.32 mm (Part No. 19091-S510) Temperature program listed above 270°C, constant flow, 68 cm/sec Helium FID 250°C

Solvents in Blood by Headspace 280°C

Constant Flow 50°C

Pressure Programming

AB C

20°C/min

Back Pressure Regulated On-Col umn

1. 2. 3. 4. 5. 6.

Methyl ethyl ketone Benzene Methyl isobutyl ketone Toluene Methyl n-butyl ketone Ethylbenzene

7. 8. 9. 10. 11.

p-Xylene m-Xylene 1-Butanol o-Xylene Styrene

110°C 40°C 10 min

7 1

Column: Injection: Oven: Detector:

420

30 min A. Pressure program: 2 psi to 7 psi at 0.3 psi/min B. Constant flow: 2 psi at 50°C C. Constant pressure: 2 psi HP-1 (Cross-Linked Methyl Silicone), 5 m x 0.53 mm x 2.65 m (Part No. 19095S-100) On column, 2 l, inlet temperature program: 50°C for 1 min, 150°C/min to 300°C Temperature program listed above ECD Pub No.: 5952-6975

0


5

5°C/min

9

2

Carrier:

3

3

10

4

5

15 Time (min)

6

8 10

11

20

1 ml blood and 100 l IS (1-butanol) Helium, 25 cm/sec 3.4 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.53 mm x 1.0 m (Part No. 19095N-123) Split (2:1), 1 ml, inlet 180°C Temperature program listed above FID 220°C

Consumer/Foods/Flavors/Fragrances

Consumer/Foods/Flavors/Fragrances Saturated Triglycerides C2 to C22 Separated on Short HP-1 column 7. 8. 9. 10. 11.

Triacetin (2:0) Tributyrin (4:0) Tricaproin (6:0) Tricaprylin (8:0) Tricaprin (10:0) Trilaurin (12:0)

Trimyristin (14:0) Tripalmitin (16:0) Tristearin (18:0) Triarachidin (20:0) Tribehenin (22:0)

1

325°C 5 min 80°C 1 min

4

Pseudomonas Aeruginosa Methyl Ester: 6. C16:1 CIS 9 1. C10:0 30H 7. C16:1 2. C12:0 8. C16:0 3. C12:0 20H 9. C18:1 4. C12:0 30H 10. C18:0 5. C14:0

15°C/min

5°C/min

170°C 5

6

7

8

9

6

10

GC: Carrier: Column: Inlet: Oven: Detector:

5

10 Time (min)

15

1

20

2 34

8

Carrier: Column:

GC: Carrier: Column: Inlet: Oven: Detector:

10

6

89

20 min

Hydrogen Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.2 mm x 0.33 m (Part No. 19091B-102) Split (100:1) Temperature program listed above FID Pub No.: 5953-1857


1. 2. 3. 4. 5. 6. 7. 8. 9.

C16:0 C16:1 C18:0 C18:1 C18:2 C18:3 C20:0 C22:0 C24:0

1. 2. 3. 4.

5. 6. 7. 8.

C16:0 C16:1n9 cis C18:0 C18:1n9 cis

C18:1n11cis C18:2n9, 12ncis C18:3n9, 12ncis, n15 cis C20:0

4

5

0

1 2 5

Derivatized Greek Olive Oil (FAMEs) Cis/Trans FAMEs Column

4

3

10

20 min

Greek Olive Oil (Methyl Ester)

2

7

3 4

9

5

6890 Hydrogen, constant flow 138 cm/sec, 1.1 psi (10 ml/min) HP-1, 5 m x 0.53 mm x 0.15 m (Part No. 19091Z-220) EPC cool on-column Temperature program listed above FID 360°C

1

5°C/min

170°C

7

11 0

270°C (2 min)

270°C (2 min)

2

3

Pseudomonas Cepa Methyl Ester: 6. C16:0 30H 1. C14:0 7. C18:1 Trans 9 2. C14:0 30H 8. C18:0 3. C16:1 CIS 9 9. C19:0 Cyclo 4. C16:0 5. C17:0 Cyclo

Applications

1. 2. 3. 4. 5. 6.

Fatty Acid Profile of Pseudomonas

1

6 7

9

8

15 20 Time (min)

25

6890 Helium, constant flow 46.6 psi HP-INNOWax, 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) 1.5 l, 260°C, deactivated liner with glass wool 150°C (1 min), 2.9°C/min 230°C (1 min) FID 260°C

2

30

0


2

4

3 5 6 Time (min)

6 7 8

8 10

Olive Oil Hydrogen at 40 cm/sec HP-23, 30 m x 0.32 mm x 0.25 m (Part No. 19091H-113) Split (100:1), 1 ml, 250°C Isothermal at 175°C FID 300°C, Nitrogen make up gas at 30 ml/min


421

Applications



Fatty Acid Profile of Salmonella Choleraesuis Methyl Ester: 1. C12:0 2. C14:0 3. DD 4. C14:0 30H 5. C16:1 CIS 9

6. 7. 8. 9.

270°C

C16:0 C17:0 Cyclo C18:1 C19:0 Cyclo

170°C

4 56

2

Trichloroesters of Fatty Acids Straight Chain

5°C/min

1. 2. 3. 4. 5. 6. 7.

8. 9. 10. 11. 12. 13.

Acetic acid Propanoic Butyric Valeric Caproic Heptanoic Octanoic

Nonanoic Decanoic Undecanoic Lauric Tridecanoic Myristic

14. 15. 16. 17. 18. 19.

Pentadecanoic Palmitic Heptadecanoic Stearic Nonadecanoic Arachidic

8

7

85°C

265°C (10 min) 7°C/min

5 min

1 1

2

3 4

15

11

5 6

9 10

7 8

12

9

17

13 14

16

18 19

3 46 min 20 min Carrier: Column:

Hydrogen Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.2 mm x 0.33 µm (Part No. 19091B-102) Split (100:1) Temperature program listed above FID Pub No.: 5953-1857


Vegetable Oil Fatty Acid Methyl Esters 1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11.

C14:0 C16:0 C18:0 C18:1 C18:2 C18:3

225°C 150°C 1 min

Hydrogen, 4 ml/min HP-5 (Cross-Linked 5% Phenyl Methyl Silicone), 30 m x 0.53 mm x 0.88 m (Part No. 19095J-023) Split (10:1), 1 l Temperature program listed above ECD


Fatty Acid Methyl Esters 260°C (7 min)

C20:0 C20:1 C22:0 C22:1 C24:0

Carrier: Column:

5°C/min

15°C/min

1. 2. 3. 4. 5.

6. 7. 8. 9.

C9:0 C10:0 C11:0 C10:20H C10:30H

6

2

10. 11. 12. 13.

C12:0 C13:0 C14:0 C15:0

8

4

14. 15. 16. 17.

C14:20H C14:30H C16:0 C17:0

C16:20H C18:0 C19:0 C20:0

12 15

2 1

3

10

3

7

9

7

13

16

11

5

4

1

17

9

10

5

8

14

11

6

0 Sample: Carrier: Column: Injection: Oven: Detector:

422

2

4

6

8 10 Time (min)

12

14

16

25 g/l in methylene chloride Helium, 40 cm/sec, 14 psi (150°C), 2.1 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (45:1), 1 l, inlet 220°C Temperature program listed above FID 275°C

3 GC: Sample: Carrier: Column: Injection: Oven: Detector:

3.5

4

4.5 5 Time (min)

5.5

6

6890 FAME Standards Helium 68.4 (64 cm/sec), constant flow HP-1MS, 20 m x 0.10 mm x 0.40 mm (Part No. 19091S-942) 1 ml 50:1, split 275°C 70°C (1 min), to 180°C at 80°C/min, to 300°C at 40°C/min, 300°C (1 min) Pub No.: 5965-7874 FID 300°C


Polyunsaturated Fatty Acid Methyl Esters 1. 2. 3. 4. 5. 6. 7. 8.

C14:0 C16:0 C16:1w7 C18:0 C18:1w9 C18:1w7 C18:2w6 C18:3w3 4

9. 10. 11. 12. 13. 14. 15. 16. 5

C20:1w9 C20:2w6 C20:3w6 C20:4w6 C20:5w3 C22:4w6 C22:5w3 C22:6w3

150°C 1 min

6

12

FAMEs C14-C24 250°C (5 min)

200°C

2°C/min

15°C/min

1. 2. 3. 4. 5.

6. 7. 8. 9.

Myristoleate Palmitate Palmitoleate Stearate Arachidate 1 2

Eicosenoate-cis-11 Behenate Erucate 180°C Nervonate 1 min

10°C/min

3 4

5

14

2

250°C (8 min)

8 6

7

7 8

11 9 10

1

0

10


15 Time (min)

13

16 15

20

25

9

0

50 g/l in methylene chloride Helium, 30 cm/sec, 10.7 psi (150°C), 1.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (60:1), 0.5 l, inlet 220°C Temperature program listed above FID 275°C


Fatty Acid Methyl Esters 1. 2. 3. 4. 5.

C3 C4 C5 C6 C7

6. 7. 8. 9. 10.

11. 12. 13. 14. 15.

C8 C9 C10 C11 C12

2

4

Sample: Carrier : Column:

6

8 10 Time (min)

12

14

16

0.1-0.2% in methyl formate Helium, 45 cm/sec, 16.8 psi (180°C), 2.3 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (20:1), 0.5 l, inlet 250°C Temperature program listed above FID 275°C


Free Fatty Acids

C14 C16 C18 C20 C22

250°C (5 min) 10°C/min

50°C 3 min

5

1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11. 12.

C2 C3 C4 C5 C5-Iso C6

13. 14. 15. 16. 17. 18.

C7 C8 C10 C12 C14 C14:1

C16 C16:1 C18 C18:1 C18:2 C18:3

250°C (5 min) 120°C 1 min

10°C/min

Applications

3

12 1

13 14 4 8 6 3

7

10

15

1 1

9 2

34 5

67 8

10

11 12 13

9

14 16

15 17 18

12

0 Sample : Carrier : Column : Injection : Oven : Detector :

5

10

15 Time (min)

20

25

0

0.1-0.2% in methyl formate Helium, 40 cm/sec, 11.8 psi (50°C), 2.6 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (100:1), 0.5 l ml, inlet 220°C Temperature program listed above FID 275°C


Time (min)

15

0.05 to 0.11% each in methylene chloride Helium 42 cm/sec, 24 psi (120°C) 1.8 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (40:1), 1 l, inlet 250°C Temperature program listed above Pub No.: 5091-6687E FID 280°C


423

Applications



Purgeable Organics in Beer 1. 2. 3. 4. 5.

6. 7. 8. 9.

Ethanol n-propanol Ethyl acetate iso-amyl alcohol Ethyl butyrate

23

1

iso-Amyl acetate Ethyl caproate Ethyl caprylate Ethyl caprate -20°C 2 min

6

4

Alcohol Beverage Standard

7

180°C 6°C/min

1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11. 12.

Acetaldehyde Acetone Ethyl formate Ethyl acetate Acetal Methanol Ethanol

8

Diacetyl 13. n-amyl alcohol sec-butanol 14. Acetic acid 15. Propionic acid n-propanol iso-butanol n-butanol 200°C (2 min) iso-amyl alcohol 60°C 4 min

6

1

6°C/min

9 5 9

10

11

12

5 13

8

4 23

14

7

15

40 min 20 min Carrier: Column:

Helium, 25 cm/sec Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.52 m (Part No. 19091B-112) Split (100:1), 1 l Temperature program listed above Pub No.: 5953-1581 FID


Brandy 1. 2. 3. 4. 5.

Carrier: Column:

Hydrogen HP-FFAP (Cross-Linked PEG-TPA), 50 m x 0.2 mm x 0.33 m (Part No. 19091F-105) Split (100:1), 1 l Temperature program listed above FID


Bourbon 6. iso-Butanol 7. 2-Methyl-1-butanol or 3-Methyl-1-Butanol 8. Acetic acid 250°C (2 min)

Acetaldehyde Ethyl acetate Methanol Ethanol n-Propanol

150°C

250°C (2 min)

4

5


424

8 5

8

23

1 10

7

6

Time (min)

20

20°C/min

5°C/min

35°C 5 min

2

6

0

Acetic acid n-Propanol iso-Butanol 2-Methyl-1-butanol or 3-Methyl-1-Butanol 150°C

7

1

5. 6. 7. 8.

Acetaldehyde Ethyl acetate Methanol Ethanol

5°C/min

35°C 5 min

4

20°C/min

1. 2. 3. 4.

3

30

Neat Helium, 33 cm/sec, 15.5 psi (35°C), 1.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (25:1), 1 l, inlet 220°C Temperature program listed above FID 280°C

0 Sample: Carrier: Column: Injection: Oven: Detector:

10

Time (min)

20

30

Neat Helium, 33 cm/sec, 15.5 psi (35°C), 1.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (25:1), 1 l, inlet 220°C Temperature program listed above FID 280°C Pub No.: 5962-0031E


Wine, Cabernet 1. 2. 3. 4. 5.

Whiskey

Acetaldehyde Ethyl acetate Methanol Ethanol n-Propanol

6. iso-Butanol 7. 2-Methyl-1-butanol or 3-Methyl-1-Butanol 8. Acetic acid 250°C (2 min) 150°C

n-Propanol Acetic acid Ethyl acetate iso-butanol

5. 6. 7. 8.

Acetaldehyde/diethylacetal 9. Ethyl octanoate Iso-amyl alcohol 10. C-10 A cid n-Amyl alcohol 11. Ethyl decanoate C-8 acid 12. C-12 Acid 13. Ethyl dodecanoate

5°C/min

35°C 5 min 4

20°C/min

1. 2. 3. 4.

1

4 6 7

8

240°C (16 min) 40°C 8 min

3 7

10°C/min

3 2 1

5

0


2

10

Time (min)

20

30

1 1

1 13 2

40 min

Neat Helium, 33 cm/sec, 15.5 psi (35°C), 1.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (25:1), 1 l, inlet 220°C Temperature program listed above FID 280°C Pub No.: 5962-0031E, 5091-9464E

Scotch 1. 2. 3. 4.

9 1 8 0

Carrier: Column:

Helium, 5 psi Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 50 m x 0.32 mm x 0.52 m (Part No. 19091B-115) Split (30:1), 1 l Temperature program listed above MSD


Perfume

Acetaldehyde Ethanol n-Propanol Ethylacetate

1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

5. Isobutanol 6. Acetal 7. 3-Methyl-1-butanol 8. 2-Methyl-1-butanol 5

2 3

Limonene Linalool Linalyl acetate Benzyl acetate Citronellol Benzene ethanol a-Methyl Ionone Carvocrol and geraniol iso-Amyl salicylate n-Amyl salicylate

11. 12. 13. 14. 15. 16. 17. 18. 19.

Commamyl acetate Acetylcedrene Diethyl phthalate 80°C Tonalid 1 min Coumarin Musk xylene Benzyl benzoate Benzyl salicylate 13 Musk ketone

8

4

10

7 3

1

1


5

7

15

12 11

16 17

14

19

6 12 min

Carrier: Column:

9

2 8

5°C/min

18 6

4

250°C (2 min)

Applications


5

6

0

Helium, 28 cm/sec HP-101 (Methyl Silicone Fluid), 50 m x 0.32 mm x 0.3 m (Part No. 19091Y-015) Split, 1 l 32°C isothermal FID Pub No.: 5963-7017E

Sample: Carrier : Column: Injection: Oven: Detector:

5

10

15 20 Time (min)

25

30

Neat Helium, 30 cm/sec, 0.9 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (10:1), 1 l, inlet 250°C Temperature program listed above MSD 280°C


425

Applications



Fragrance Standard Sample 250°C (5 min) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.


Ethyl acetate Diacetyl cis-3-Hexanol trans-2-Hexanal Prenyl acetate Benzaldehyde Limonene Maltol Octanol cis-3-Hexenylacetate p-Methyl cresol p-Cresol Nonanal Linalool Rose oxide Camphor Benzyl acetate Menthol alpha-Terpineol

20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38.

Decanal Citronellol Cinnamic aldehyde Geraniol Indole Heliotropine Phenylpropyl acetate Eugenol Citronellyl acetate Vanillin Dimethyl anthranilate Cyclamen aldehyde Lilial Musk xylol Helional Methyl naphthyl ketone Hedione Isopropyl myristate Ethylene brassylate

11

50°C

5°C/min

1 min 17 35

38

29 5 14

16

28

15

1

36 27 20 21 19 30 18 31

6 7 12

Helium, 1 ml/min constant flow HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.25 mm x 0.25 m (Part No. 19091Z-133) 1 l, Agilent 7673 with 250 m nanoliter adapter on-column injection; syringe (Part No. 5181-3321) Temperature program listed above MSD 280°C

22

23

5.00

10.00

15.00 20.00 Time (min)

37

25.00

30.00

Fragrance Standard Mix 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

1-Limonene cis-3-Hexenal Prenyl acetate cis-3-Hexenyl acetate Rose oxide Hexenol Nonanal p-Methyl cresol Decanal Camphor Benzyl aldehyde Linalool Octanol Menthol Citronellyl acetate alpha-Terpineol Benzyl acetate Citronellol Geraniol

20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37.

Phenyl propyl acetate Maltol Cyclamen aldehyde Cinnamic aldehyde Isopropyl myristate Unknown Dimethyl anthranilate p-Cresol Eugenol Heliotropine Helional Indole Methyl naphthyl ketone Musk xylol Vanillin Ethylene brassylate Lilial Hidione

250°C (2 min) 80°C

9

33

18 25 26 20

5

4

13 11

5

35

21

31

24 7 10

14 15 12 17

34

29

19 22 23

27 28 30

8


426

500 ppm in ethanol Helium, 30 cm/sec, 0.9 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 0.00 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) 0.2 l, Agilent 7673 with, nanoliter adapter, on-column Temperature program listed above MSD 280°C

5.00

36

16

6

3

2


5°C/min

1 min

32

10.00

15.00 20.00 Time (min)

25.00

30.00


Man's Cologne 1. 2. 3. 4. 5. 6.

L-Limonene Unknown Linalool Linalyl acetate 1,2-Pronanediol Menthol

7. 8. 9. 10. 11. 12.

1-Octanol, 2,7-dimethyl alpha-Terpineol Benzyl acetate Geranyl acetate beta-Citronellol Geraniol 3

5

38. 39. 40. 41. 42. 43.

Benzyl alcohol Phenyl ethyl alcohol Anis aldehyde iso-Amyl salicylate Amyl salicylate Patchouli alcohol

6

13. 14. 15. 16. 17. 18.

11

Heliotropine OTMS Diethyl phthalate Coumarin Vanillin Musk ambrette

19. 20. 21. 22.

Benzyl benzoate Musk T Musk ketone Benzoic acid

250°C 2 min 80°C 1 min

5°C/min

25

19 23

1

9

27

22 12

4

7

8

10

15 13 14

16

18

24

20

28

5.00 10.00 15.00 20.00 25.00 30.00 Time (min) Neat Helium, 30 cm/sec, 0.9 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.25 mm x 0.25 m (Part No. 19091N-133) Split (10:1), 1 l, inlet 250°C Temperature program listed above MSD 280°C

Flavor Mixture Fenchone Thujone Benzaldehyde trans-Carveol Farnesol cis-Carveol trans-Geraniol

35.00

Cosmetics by Headspace 8. 9. 10. 11. 12. 13.

210°C 2 min

Citral Eugenol Vanillan trans-Isoeugenol trans-Citronellyl tiglate cis-Citronellyl tiglate

80°C 1 min

8°C/min

1. 2. 3. 4. 5. 6.

Cineole Limonene Menthone Menthol Carvone Anethole

200°C (5 min) 40°C 5 min 2

13

9

2

3 3


5

5

8

Time (min)

6

10

7 46

4

1

5

1

0

8°C/min

Applications

Sample: Carrier : Column: Injection: Oven: Detector:

1. 2. 3. 4. 5. 6. 7.

26

21

17

12 11

10

15

Helium, 90 kPa., 2.2 ml/min constant flow Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.52 m (Part No. 19091B-112) Split (20:1), 1 l, inlet 250°C Temperature program listed above IRD, 280°C, Wide Band MCT, 550 to 4000 cm-1


Helium, 20 cm/sec PONA (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.5 m (Part No. 19091S-001) Split (20:1), headspace, 1 l Temperature program listed above Pub No.: 5953-8063 MSD


427

Applications



Strawberry Syrup 1. 2. 3. 4. 5. 6.

Peppermint Oil

7. Ethyl benzoate Ethyl acetate 8. Citronellol Ethyl butyrate iso-Amyl acetate 9. Geraniol 10. Ethyl-3-phenyl oxiran carboxylate Amyl acetate 250°C (2 min) iso-Amyl butyrate 11. Strawberry aldehyde 12. Benzyl benzoate Amyl butyrate 60°C 10°C/min 1 min 2 34 1

1. 2. 3. 4.

5. 6. 7. 8.

Limonene Cineole Menthone Menthofuran

d-iso-Menthone Menthyl acetate Menthol Germacrene

250°C 10°C/min

60°C 1 min 7

3 2

11

4

5

6

10

6

1

5

7

8

12 89

0


5

10 Time (min)

15

0

20

Neat syrup Helium, 40 cm/sec, 11.7 psi (60°C), 2.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (60:1), 0.2 l, inlet 220°C Temperature program listed above FID 275°C


2

4

6

8 10 Time (min)

12

14

Neat Helium, 40 cm/sec, 11.7 psi (60°C), 2.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.32 mm x 0.5 m (Part No. 19091N-213) Split (60:1), 0.5 l, inlet 220°C Temperature program listed above FID 275°C

Analysis of Resoloids

Beeswax Absolute

360°C 90°C 40°C 1 min

Styrex Resin

Benzoin Siam


428

Helium 37 cm/sec (40°C), 1.8 ml/min constant flow HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.25 mm x 0.25 m (Part No. 19091Z-433) On-column,1 l 50°C (0.1 min) to 370°C (5 min) at 50°C/min Temperature program listed above FID 375°C

16

25°C/min

10 min


SFE of Conifer Species 1. 2. 3. 4.

Tricyclene a-Pinene Camphene Bromononane (ISTD)

SFE of Fragrance in Liquid Detergent 275°C

5. Bornyl acetate 6. Diterpene 7. Diterpene

250°C 280°C (5 min)

220°C 50°C

20°C/min

10°C/min

3°C/min

35°C 1 min

trans-geraniol

4°C/min

-citronellol

1

benzyl iso-amyl ether

linalool 2

Carbon = 496 nm

5

4

Orange R

7

6

-myrcene

3

0


10

20

30 Time (min)

40

50

Helium HP-1 (Cross-Linked Methyl Silicone), 25 m x 0.32 mm x 0.17 m (Part No. 19091Z-012) 1 l, inlet 280°C (0.80/ml extract density) Temperature program listed above AED

Beeswax


20

30

40 50 Time (min)

60

70

Helium 28 cm/sec, 28.5 psi (35°C), 1.0 ml/min constant flow PONA, 50 m x 0.2 mm x 0.5 m (Part No. 19091S-001) 1 m x 0.53 mm retention gap (Part No. 19095-60610) On-column,1 l, oven track mode Temperature program listed above MSD 280°C

TMS Derivatives of Monosaccharide 230°C (10 min)

350°C (10 min) 130°C

10°C/min

5°C/min

0.5 min

Applications

150°C


20 min

Helium, 8 psi Ultra 1 (Cross-Linked Methyl Silicone), 25 m x 0.2 mm x 0.11 m (use 6 m only), (Part No. 19091A-002) Split, 1 l Temperature program listed above FID


Helium, 16 psi Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.2 mm x 0.33 m (Part No. 19091A-102) Split, 1 l Temperature program listed above MSD


429

Applications



Sulfur and Selenium in Garlic by Headspace 1. 2. 3. 4. 5. 6. 7. 7a.

8. 9. 10. 11. 12. 13. 14.

CH3-Se-CH3 C3H5-S-CH3 C3H5-Se-CH3 CH3-S-S-CH3 C3H5-S-C3H5 CH3-S-Se-CH3 C3H5-S-S-CH3 CH3-Se-Se-CH3

CH3-Se-S-C3H5 CH3-S-S-S-CH3 C3H5-S-S-C3H5 CH3-S-Se-S-CH3 CH3-S-S-S-C3H5 C3H5-S-S-S-C3H5 C3H5-S-S-S-C3H5

230°C 5 min 35°C 3 min

(B)

5°C/min

2

(A)

10

AED

2

4

S

10

7

5

12

1

13

3

9.00 10.00

11

7a

0

10

Time (min)

9

6

8 1

3 4

9 6

Carrier:

20

30

8

11

15.00 20.00 Time (min)

25.00

(A): Helium, 28 cm/sec, 18 psi (35°C), 1.2 ml/min constant flow (B): Helium, 28 cm/sec, 5.6 psi (35°C), 1.2 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 60 m x 0.32 mm x 0.25 m (Part No. 19091N-116) Splitless, SI inlet (140°C), Temperature program listed above AED 190°C and MSD 230°C


SFE of Hops

13

12

7

C

Se

MSD

5

Food Label by Headspace 250°C

1. Myrcene 2. b-Caryophyllene 3. Humulene

50°C

210°C (5 min) 40°C

5°C/min

3 min 1

2

6°C/min

3

0

10

Time (min)

20

30


430

Helium, 10 psi HP-5 (Cross-Linked 5% Phenyl Methyl Silicone), 30 m x 0.53 mm x 0.88 m (Part No. 19095J-023) On-column, 1 l Temperature program listed above FID 300°C Pub No.: 5952-2342


One 1 x 2 inch label Helium, 35 cm/sec 4 psi (40°C), 5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 30 m x 0.53 mm x 1.0 m (Part No. 19095N-123) Split (10:1), 1 ml Headspace, inlet 220°C Temperature program listed above FID 300°C

Environmental

Environmental EPA 624 Volatile Organics 1. 2. 3. 4. 5.

Chloromethane vinyl chloride Bromoethane chloroethane Trichlorofluoromethane 1,1-Dichloroethylene Methylene chloride

6. 7. 8. 9. 10.

18. 19. 20. 21. 22.

trans-1,2-Dichloroethylene 1,1-Dichloroethane(IS) Bromochloromethane Chloroform 1,1,1-trichloroethane Carbon tetrachloride

Benzene 1,2-dichloroethane 1,4-Difluorobenzene(IS) Trichloroethene 1,2-Dichloropropane Bromodichloromethane

cis-1,3-Dichloropropene Toluene trans-1,2-Dichloropropene 1,1,2-Trichloroethane tetrachloroethene 27. Dibromochloromethane 28. Chlorobenzene

23. 24. 25. 26.

11. 12. 13. 14. 15. 16. 17.

140°C (5 min)

21 35°C

11

3 4 1 2

0

2

12 13 15 14

16

7

4

6

27

19

26

8

10

28

22

20

9 8 10

6 5

5°C/min

1 min

24 17

Ethyl benzene Bromoform Bromofluorobenzene(SS) 1,1,2,2-Tetrachloroethane 1,3 Dichlorobenzene 1,4-Dichlorobenzene 1,2-Dichlorobenzene

23

18

Carrier: Column:

25

Hydrogen, 39 psi, 5.3 ml/min (35°C) HP-624, 30 m x 0.32 mm x 1.8 m (Part No. 19091V-413) Split (20:1), 1 l, inlet 200°C 35°C (1 min) to 140°C (5 min) at 5°C/min FID 2505C


12 14 Time (min)

16

18

20

22

Purgeable Halocarbons Chloromethane Vinyl chloride Bromoethane Chloroethane 1,1-Dichloroethylene Methylene chloride

7. 8. 9. 10. 11. 12.

trans-1,2 Dichloroethylene 1,1-Dichloroethane Bromochloromethane Chloroform 1,1,1-Trichloroethane Carbon tetrachloride 1

3 45

1,2-Dichloroethane Trichloroethylene 1,2-Dichloropropane Bromodichloromethane cis-1,3-Dichloropropene

12

7 2

13. 14. 15. 16. 17.

15

16 17

18. 19. 20. 21. 22.

18 22

trans-1,3-Dichloropropene 1,1,2-trichloroethane Tetrachloroethylene Dibromochloromethane Chlorobenzene

25

26

23. 24. 25. 26. 27.

Bromoform 1,1,2,2-tetrachloroethane 1,3 Dichlorobenzene(m) 1,4-Dichlorobenzene(p) 1,2-Dichlorobenzene(o)

Applications

1. 2. 3. 4. 5. 6.

27

8 6

14

19

140°C (5 min)

24

20

10

35°C

5°C/min

1 min 9

21

11

23

13 0


20 Time (min) Hydrogen, 39 psi, 5.3 ml/min (35°C) HP-624, 30 m x 0.32 mm x 1.8 m (Part No. 19091V-413) Split (20:1), 1 l, inlet 200°C Temperature program listed above FID 250°C


30

431

Applications

Environmental


Purgeable Organic Compounds 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26.

Dichlorodifluoromethane Chloromethane Vinyl chloride Bromomethane Chloroethane Trichlorofluoromethane 1,1-Dichloroethene Methylene chloride trans-1,2-Dichloroethene 1,1-Dichloroethane 2,2-Dichloropropane cis-1,2-Dichloroethene Bromochloromethane

Chloroform 1,1,1-Trichloroethane 1,1-Dichloropropene Carbon tetrachloride Benzene 1,2-Dichloroethane Fluorobenzene ISTD Trichloroethene Bromodichloromethane cis-1,3-Dichloropropene Toluene trans-1,3-Dichloropropene 1,1,2-Trichloroethane

27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39.

40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52.

Tetrachloroethene 1,3-Dichloropropene Dibromochloromethane 1,2-Dibromomethane Chlorobenzene 1,1,1,2-Tetrachloroethane Ethylbenzene m-Xylene 1,2-Dichloropropane Dibromomethane p-Xylene o-Xylene Styrene

Bromoform Isopropylbenzene 4-Bromofluorobenzene SURR Bromobenzene 1,1,2,2-Tetrachloroethane 1,2,3-Trichloropropane n-Propylbenzene 2-Chlorotoluene 4-Chlorotoluene 1,3,5-Triethylbenzene tert-Butylbenzene 1,2,4-Trimethylbenzene sec-Butylbenzene

53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63.

By Purge and Trap (EPA Method 524.2/6234/8260) Extracted ion chromatogram

7

1

2 3 45

6

8

9 10

5.00 Carrier: Column: Injection: Oven: Detector:

18, 19 21 20

16, 17 11, 12

220°C

10.00

20°C/min

160°C

26 25

1,3-Dichlorobenzene 4-Isopropyltoluene 1,4-Dichlorobenzene 1,2-Dichlorobenzene-d4 SURR 1,2-Dichlorobenzene n-Butylbenzene 1,2-Dibromo-3-chloropropane 1,2,4-Trichlorobenzene Hexachlorobutadiene Naphthalene 1,2,3-Trichlorobenzene

38, 39

34, 35 29, 30

44, 45 43

5 min

54, 55

42

28 27

15.00

50

60

40

61, 62 63

59 25.00

20.00

Time (min)

6 min 6°C/min

35°C

56, 57, 58

36, 37

Helium, 8.5 ml/min HP-624, 30 m x 0.53 mm x 3 m (Part No. 19095V-423) Use jet separator (Part No. 59913A, #007) Temperature program listed above MSD

30.00

Pub No.: 5091-0677, 5091-2477E, 5091-5909

Purgeable Halocarbons and Aromatics 1. 2. 3. 4. 5.

Chloroethane 1,1-Dichloroethane Methylene chloride trans-1,2-Dichloreoethene 1,1-Dichloreoethane

6. 7. 8. 9. 10.

Bromochloromethane Chloroform 1,1,1-Trichloroethane Carbon tetrachloride 1,2-Dichloroethane

11. 12. 13. 14. 15.

Trichlooethene 1,2-Dichloropropane Bromodichloromethane trans-1,3-Dichloropropene cis-1,3-Dichloropropene

22

18 PID

11 14 2

23 5

ELCD 1

4

8

24

15

18

35°C

23 24 21 22

5 min

5°C/min

19

0

432

1,1,2,2-Tetrachloroethane 1,3-Dichlorobenzene 1,4-Dichlorobenzene 1,2-Dichlorobenzene

140°C 5 min

9


21. 22. 23. 24.

15

7 10 12 1314 16 17 11 6

1,1,2-Trichloroethane Tetrachloroethene Dibromochloromethane Chlorobenzene Bromoform

23

16

4

16. 17. 18. 19. 20.

Helium, 8.5 ml/min HP-624, 30 m x 0.53 mm x 3 m (Part No. 19095V-423) Tekmar LSC-2 Purge & Trap System, Tenax Trap, Packed injection port (250°C) Temperature program listed above ELCD; PID

20

30 min

Pub No.: 5954-7613

Environmental

Purgeable Halocarbons and Aromatics 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Dichlorodifluoromethane 13. Chloroform 25. Toluene 37. o-Xylene 49. 1,2,4-Trimethylbenzene 38. Styrene 50. sec-Butylbenzene Chloromethane 14. Bromochloromethane 26. trans-1,3-Dichloropropene 39. Isopropyl benzene Vinyl chloride 15. 1,1,1-Trichloroethane 27. 1,1,2-Trichloroethane 51. p-Isopropyltoluene 40. Bromoform Bromomethane 16. 1,1-Dichloropropene 28. 1,3-Dichloropropane 52. 1,3-Dichlorobenzene 41. 1,1,2,2-Tetrachloroethane Chloroethane 17. Carbon tetrachloride 29. Tetrachloroethene 53. 1,4-Dichlorobenzene Trichlorofluoromethane 18. 1,2-Dichloroethane 30. Dibromochloromethane 42. 1,2,3-Trichloropropane 54. n-Butylbenzene 1,1-Dichloroethene 31. 1,2-Dibromoethane 19. Benzene 43. n-Propyl benzene 55. 1,2-Dichlorobenzene 20. Trichloroethene Methylene chloride 32. Chlorobenzene 44. Bromobenzene 56. 1,2-Dibromo-3-chloropropane 21. 1,2-Dichloropropane 57. 1,2,4-Trichlorobenzene trans-1,2-dichloroethene 33. 1,1,1,2-Tetrachloroethane 45. 1,3,5-Trimethylbenzene 1,1-Dichloroethane 22. Bromodichloromethane 34. Ethyl benzene 46. 2-Chlorotoluene 58. Hexachlorobutadiene 23. Dibromomethane 35. m-Xylene 59. Naphthalene 2,2-Dichloropropane 47. 4-Chlorotoluene 24. cis-1,3-Dichloropropene 60. 1,2,3-Trichlorobenzene cis-1,2-Dichloroethene 36. p-Xylene 48. tert-Butylbenzene By Purge and Trap (EPA Method 502.2/8020/8021) 35, 36 44 200°C 10, 11 RT=48.5 min ELCD PID RT=48.6 13 45, 46 27 32, 34 20 14 52 47, 48 32 33 35°C 21 58 3°C/min 29 18 49 53 59 10 min 40 22 28 15 55 37,38 8 53 57 60 16 55 54 10 43 41 52 12 20 57 60 31 39 50 23 7 9 9 47 29 58 24 16 46 42 7 26 24 56 1 44 5, 6 3 26 234 1 10 Carrier: Column: Injection:

10 30 40 50 Time (min) Helium, 30 cm/sec, 10 ml/min constant flow HP-VOC, 105 m x 0.53 mm x 3 m (Part No. 19095R-420) O.I. 4460 Purge/Trap, 5 ml water sample

20

20

30 40 50 Time (min) Oven: Temperature program listed above Detector: ELCD 900°C and PID 250°C

Purgeable Halocarbons and Aromatics

150°C 55°C -70°C 4 min

19

9

25 +26 35 +36 53 50 52 54 51 59 44 49 46 32 43 57 60 38 39

230°C 20°C/min

34

3°C/min

7

20°C/min

20

16

6

3

29 24

20

10

30 Time (min)

2

Sample: Carrier: Column: Oven: Detector: Peak ID:

1

0

10

58

4

PID 0

12

20

Time (min)

30

37

Applications

By Purge and Trap with Cryo Focusing

40

40

50

16 ppb each component in 5 ml water Helium, 3 ml/min constant flow HP-VOC, 90 m x 0.32 mm x 1.8 m (Part No. 19091R-319) Temperature program listed above PID 250°C and ELCD 850°C See above

50


433

Applications

Environmental


Chlorinated Pesticides 1

EPA - 608 Targeted Compounds 2

5

9

6

20 11

7 10

4

1A. Splitless Injection

12

15 14 18 13 1617

3

a 6

10

8 1 2

12

14

16

18

20

5 6

7 10

4

1. 2. 3. 4. 5. 6. 7. 10. 11. 12. 13. 14. 15. 16. 17. 18.

-BHC -BHC -BHC Heptachlor -BHC Aldrin Heptachlor epoxide Endosulfan I 4,4'-DDE Dieldrin Endrin 4,4'-DDD Endosulfan II 4,4'-DDT Endrin aldehyde Endosulfan sulfate

12

14 15 18 13 16 17

Carrier: Column :

Helium, 20 cm/sec, 2.2 psi (80 ), 2.4 ml/min constant flow HP-608, 30 m x 0.53 mm x 0.5 m (Part No. 19095S-023) Splitless, 1 l, inlet 250°C on column, 1 l, oven-track mode Temperature program listed above ECD 330°C Pub No.: 5963-2003

Injection: a 10

8

12

14

16

18

Oven: Detector: 20

Volatile Halocarbons by Headspace 1. 2. 3. 4. 5.

Chloroform 1,1,1-Trichloroethane Carbon tetrachloride Bromodichloromethane 1,1,2-Trichloroethane

6. 7. 8. 9. 10.

30°C/min 85°C

1B. On-Column Injection

11

3

6

250°C 5°C/min

195°C

Purgeable Aromatics (BTX) by Headspace

Dibromochloromethane Tetrachloroethylene Bromoform o-dichlorobenzene 2,4-Trichlorobenzene

1. 2. 3. 4.

Benzene Toluene m-+p-Xylene o-Xylene

130°C (2 min) 40°C

3

10°C/min

3 min 100°C (8 min)

7 40°C

4

10°C/min

4 min 3

1

1

6

2

4

2 8 5

10 9

14 min


434

Helium, 49 cm/sec HP-1, 25 m x 0.32 mm x 0.52 m (Part No. 19091J-112) Split (64:1), headspace 10 ppb/component Temperature program listed above ECD


Helium, 24 cm/sec HP-1 (Cross-Linked Methyl Silicone), 25 m x 0.32 mm x 0.52 m (Part No. 19091Z-112) Headspace (Agilent 19395A) split (50:1), 1 l Temperature program listed above FID Pub. No.: 5953-1820

Environmental

Analysis of Volatile Organics 1. 2. 3. 4. 5.

Trihalomethanes (THMS)

trans-1,2-Dichloroethene Carbon tetrachloride Benzene Tetrachloroethene Ethylbenzene

140°C 35°C

3

150°C 1

50°C

2

10°C/min

3

H

1

1

5

23

Chloroform Dichlorobromoform Chlorodibromoform Bromoform

15°C/min

4 min

5

1. 2. 3. 4.

2

4 CI

C

4

8 min 1

4 8 min 8 min Helium, 0.134 ml/min (29 cm/sec) HP-5 (Cross-Linked 5% Phenyl Methyl Silicone), 10 m x 0.10 mm x 0.34 m (Part No. 19091S-003) Split (1200:1), 1 l Temperature program listed above Pub. No.: 5954-8711 AED


Fast Chromatography 60 VOC Compounds 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Trichlorofluoromethane 1,1-Dichloroethene 1,1-Dichloroethene Benzene 1,2-Dichloropropane Toluene 1,2-Dibromoethane o-Xylene 1,4-Dichlorobenzene Naphthalene

Pollutants in a Chemical Dump Site 220°C

35°C

15°C/min

4 min


10 min Hydrogen, 10 kPa HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.53 mm x 2.65 m (HP Part No. 19095Z-123) Retention Gap: 1.5 m x 0.53 mm (Part No. 19095-22010) 1 l, on-column, oven track mode Temperature program listed above ECD Pub No.: 5091-1657

5. 6. 7. 8. 9. 10.

1. Chlorotoluene 2. Benzaldehyde 3. A. 1,4-Dichlorobenzene 4. B. Nitrobenzene

1 ppb

2 GC: Sample: Carrier: Column: Injection: Oven:

4 3

4

35°C 5 min

8°C/min

B 8

2

6

A 3

1

3

235°C

1

9

1

Chlorobenzaldehyde Benzyl chloride Dichlorotoluene Bornyl chloride Trichlorobenzene Chlorobenzyl chloride

Applications

Carrier: Column:

Carrier: Column:

6

6

8 Time (min)

5973 GCD 10 ml split (ratio 40:1) Helium, 0.65 ml/min constant flow HP-624, 25 m x 200 m x 1.12 m, (Part No. 19091V-402) HP 7695 Purge & Trap Temperature program shown above

12

14

(nm) Carbon 495.7

8

7 5 6 3 8 4

7 10

5 7

A

10 5

4

16

5

15

Time (min)

Chlorine 479.5

20

25

Carrier: Column:

Pub No.: 5965-6692

Helium, 0.75 ml/min Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.2 mm x 0.11 µm (Part No. 19091B-002) Injection: Split (40:1), 1 µl Oven: Temperature program listed above Pub No.: 5959-8709 Detector: AED


435

Applications

Environmental


Semivolatile Organics: EPA Method 525 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Acenaphthene-d10 Hexachlorocyclopentadiene Dimethylphthalate Acenaphthalate 2-Chlorobiphenyl Fluorene Diethylphthalate 2,3-Dichlorobiphenyl Hexachlorobenzene Simazine Atrazine Phenathrene-d10

13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24.

Pentachlorophenol Lindane Phenanthrene Anthacene 2,4,5-trichlorobiphenyl Heptachlor Alachlor Di-n-butylphthalate 2,2',4,4'-tatrachlorobiphenyl Aldrin Heptachlor epoxide 2,2',3',4,6-pentachlorobiphenyl

1

25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35.

300°C (5 min) 6°C/min

160°C 30°C

45°C 1 min

12

4

5.00

15.00

10.00

45 46 44

25.00

20.00

2,2',3,3',4,4',6'-heptachlorobiphenyl Methoxychlor 2,2',3,3',4,5',6,6'-octachlorobiphenyl Di(2-ethylhexyl)phthalate Benzo[b]fluoranthene Benzo (k) fluoranthene Benzo[a]pyrene Perylene-d12 Indeno{1,2,3,c,d}pyrene Dibenz[a,h]anthracene Benzo[g,h,i]perylene

GC: Column:

32 43 5 6 15 16 3 18 9 26 35 7 33 13 1719 20 25 39 41 9 24 22 29 31 34 38 2 42 11 37 40 23 28 30

36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46.

Gamma-chlordane Pyrene Alpha-chlordane Trans nonachlor 2,2',4,4',5,6'-hexachlorobiphenyl Endrin Butylbenzylphthalate Chrysene -d12 Di(2-ethylhexyl)adipate Benz (a) anthracene Chrysene

30.00

Time (min)

6890 HP-5MS, 30 m x 0.25 mm x 0.25 µm (Part No. 19091S-433) Temp: 250°C Carrier gas: Helium Inj. port: Splitless, 6 µl Inj. technique: Agilent 7673B automated sampler, fast injection Volume injected: Splitless, 1µl Inlet press. Prog: 35 psi (0.2 min) at 40 psi/min to 7.4 psi (1 ml/min constant flow) Purge on: 1 min Initial temp: 45°C (1 min) Oven: Temperature program listed above Transfer line: 280°C capillary direct Solvent delay: 3.5 min Mass range: 5-450 Detector: MSD Pub No.: 5963-2003

Semivolatiles Water Analysis, GC/MS 1. 2. 3. 4. 5. 6. 7. 8. 9.

10. 11. 12. 13. 14. 15. 16. 17. 18.

Bis (2-chloroethyl) ether Dichlorobenzene, 1,3Dichlorobenzene, 1,4Dichlorobenzene, 1,2Bis (2-chloroisopropyl) ether N-nitroso di-n-propylamine Hexachloroethanene Nitrobenzene Isophorone

Bis (2-chloroethoxy) methane Trichlorobenzene, 1,2,4Naphthalene Hexachlorobutadiene Hexachlorocyclopentadiene 2-Chloronaphthalene Acenaphthylene Dimethyl phthalate Dinitrotoluene, 2,6-

310°C (5 min)

15

11, 12

1

10 13

14

7 23 8 4

16

34

28

19

9 6

31

7°C/min

80°C

21

27

24

17

25

18

26

Acenaphthene Dinitrotoluene, 2,4Fluorene Diethyl phthalate 4-Chlorophenyl phenyl ether N-Nitrosodiphenylamine Azobenzene 4-Bromophenyl phenyl ether Hexachlorobenzene

28. 29. 30. 31. 32. 33. 34. 35. 36.

29

30

33

36

38 35 37 39

40 41

20

436

10.00

15.00

Time (min)

44 43 42

5

5.00

Phenanthrene Anthracene Di-n-butylphthalate Fluoranthene Pyrene Butylbenzyl phthalate Benz (a) anthracene Chrysene Dichlorobenzidine, 3,3'-

32

22, 23

4 min

19. 20. 21. 22. 23. 24. 25. 26. 27.

20.00

25.00

30.00

37. 38. 39. 40. 41. 42. 43. 44.

Bis-(2-ethylhexyl) phthalate Di-n-octyl phthalate Benzo (b) fluoranthene Benzo (k) fluoranthene Benzo (a) fluoranthene ndeno (1,2,3-c,d) pyrene Dibenz (a,h) anthracene Benzo (g,h,i) perylene

Temperature: 83°C, 50°C/min to 315°C (5 min) Volume injected: 1 ml Column: HP-5MS, 30 m x 0.25 mm x 0.25 m (Part No. 19091S-433) Carrier gas: Helium, 33 cm/sec (1 ml/min) constant flow Oven: Temp. Program listed above Initial temperature: 80C for 4 min Detector: MSD, 280°C

Environmental

Polynuclear Aromatic Hydrocarbons 1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11.

Naphthalene Acenaphthylene Acenapthene Fluorene Phenanthrene Anthracene

Fluoranthene Pyrene Benzo(a)anthracene Chrysene Benzo(b)fluoranthene

Polynuclear Aromatic Hydrocarbons 12. 13. 14. 15. 16.

Benzo(k)fluoranthene Benzo(e)pyrene Indeno(1,2,3)pyrene Dibenzoanthracene Benzo(g,h,i)perylene

1. 2. 3. 4. 5. 6.

300°C 3°C min

160°C

Naphthalene Acenaphthylene Acenaphthene Fluorene Phenanthrene Anthracene

1 234 7 8

7. 8. 9. 10. 11.

Fluoranthene Pyrene Benzo(a)anthracene Chrysene Benzo(b)fluoranthene

56

10

5 GC: Carrier: Column: Inlet: Oven: Detector:

7

8

15

910

9 10

20

25

11 12 1112

35

30

Time (min) 6890 Hydrogen, constant flow, 10.2 psi, flow 1.5 ml/min HP-5, 30 m x 0.25 mm x 0.25 µm HP-5MS (Part No. 19091S-433) 1 µl 0.01 mg each/ml, split 25/1, 325°C Temperature program listed above FID 325°C

300°C 3°C min

80°C 25°C/min 1.5 min

13

15 14 16

13

Benzo(k)fluoranthene Benzo(e)pyrene Indeno(1,2,3)pyrene Dibenzoanthracene Benzo(g,h,i)perylene 160°C

80°C 25°C/min 1 min

1 23 4

12. 13. 14. 15. 16.

15 14 16

40 GC: Sample: Carrier: Column: Injection: Oven: Detector:

Pub No.: 5965-7874

10

20

30

40

Time (min) 6890 PAH Standards Hydrogen (40 cm/sec), Constant Flow, initial pressure-9 psi HP-5TA, 30 m x 0.25 mm x 0.25 µm (Part No 19091M-433) 1 µl, 100:1 split Temperature program listed above Pub No.: 5965-7874 FID 325°C

Pesticides, Standard Mix Tetrachloro-m-xylene (SS1) alpha-BHC Lindane beta-BHC Heptachlor delta-BHC Aldrin Heptachlor epoxide Endosulfan I 4,4'-DDE

11. 12. 13. 14. 15. 16. 17. 18. 19. 20.

Dieldrin Endrin 4,4'-DDD Endosulfan II 4,4'-DDT Endrin aldehyde Endosulfan sulfate Methoxychlor Endrin ketone Decachlorobiphenyl (SS2) 1

280°C 160°C 10°C/min 1 min

3 20

2 6

7 8

45

0 GC: Column: Detector: Carrier gas: Injection: Oven:

2


Applications

1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

4

9

6

11 10

Time (min)

18 14 16 17 13 12 15

8

19

10

12

5890 Series II with EPC HP-35, 30 m x 0.53 mm x 1.0 m ECD Pressure program: 8.6 psi (1 min) at 0.5 psi/min to 12 psi and at 3.0 psi/min to 25 psi (0 min) Splitless, 1 l, purge delay, 0.75 min, inlet temperature of 250C Temperature program listed above


437

Applications

Environmental


Organochlorine Pesticides (Dual Column Analysis) EPA - 608 Targeted Compounds 9. Dialdrin 1. alpha-BHC 10. 4,4'-DDE 2. beta-BHC 11. Endrin 3. gamma-BHC 12. Endosulfan II 4. delta-BHC 13. 4,4'-DDD 5. Heptachlor 14. Endrinaldehyde 6. Aldrin 7. Aeptachlorepoxide 15. Endosulfan sulfate 16. 4.4'-DDT 8. Endosulfan 1

205°C 180°C 80°C 1 min

4 min

250°C


5°C/min

195°C 30°C/min 85°C

30°C/min

9

6

3 10

1

7 2

1 9 10 11 12 13 16 14

6 5

11

8

4

3

2

8

4

15

Carrier: Column:

Helium, 12.4 psi PAS-1701 25 m x 0.32 mm x 0.25 µm (Part No. 19091S-011) Splitless, 200 ppb (main components), 1 ml Temperature program listed above ECD


Etridiazole Chloroneb Propachlor Trifluralin a-BHC Hexachlorobenzene ß-BHC O-BHC Y-BHC Chlorothalonil 6 5 Heptachlor Aldrin DCPA

14. 15. 16. 17. 18. 19. 20. 21.

15

10 13

8

1

14 11

180°C

5 GC: Carrier: Column: Injection:

438

5890 EPC Helium, 45 cm/sec (80°C) constant flow HP-5MS (Part No. 19091S-433) Splitless, 1 µl


8. 9. 10. 11. 12. 13. 14. 15. 16.

1

21 23 24 22

Time (min) 15

25 26

Endosulfan 1 Dieldrin 4,4′-DDE Endrin Endosulfan II 4,4′-DDD Endrin aldehyde Endosulfan sulfate 110°C 4,4′-DDT 1 min 13

3 5

2°C/min 16

9 10

7

11

27 29

15

160°C 3°C/min

133°C

14

6

18 19

20

10

alpha-BHC beta-BHC Lindane delta-BHC Heptachlor Aldrin Heptachlor epoxide

16

2 3

30°C/min

4 min 3°/ min

1. 2. 3. 4. 5. 6. 7.

4

12

7

Chlorobenzilate 4,4'-DDD Endrin aldehyde Endosulfan sulfate 4,4'-DDT Methoxychlor cis-Permethrin trans-Permethrin 205°C

17 80°C 1 min

9

4

Chlorinated Pesticides 22. 23. 24. 25. 26. 27. 28. 29.

Heptachlor epoxide Y-Chlordane Endosulfan I a-Chlordane Dieldrin 4,4'-DDE Endrin Endosulfan II

Helium, 15.7 psi PAS-5 25 m x 0.32 mm x 0.52 µm (Part No. 19091S-010) Splitless, 200 ppb (main components), 1 µl Temperature program listed above ECD Pub. No.: 5952-2341


Chlorinated Pesticides: EPA Method 508 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

16 13 12 15 14

25 min

25 min Carrier: Column:

7

5

8

12

28

20

Purge delay: 1 min Head pressure:24 psi Oven: Temperature program above Makeup gas: Nitrogen, 60 ml/min Anode purge: 3 ml/min Detector: ECD 320°C


23 min Helium, 30 psi Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone) 25 m x 0.2 mm x 0.11 µm (Part No. 19091B-002) Splitless, 0.5 µl Temperature program listed above Pub No.: 5952-0209 ECD

Environmental

Organochlorine Pesticides and PCBs: CLP Pesticides Tetachloro-m-xylene 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

a-BHC Y-BHC ß-BHC Heptachlor O-BHC Aldrin Heptachlor epoxide Y-Chlordane a-Chlordane Endosulfan I 4,4′-DDE

190°C

12. Dieldrin 13. Endrin 14. 4,4′-DDE 15. Endosulfan II 16. 4,4′-DDT 17. Edrin aldehyde 18. Endosulfan sulfate 19. Methoxychlor 20. Endrin ketone SS2.Decachlorobiphenyl

80°C 30°C/min 1 min

SS2

SS1

11

1 GC: Carrier: Column: Vol. inj.: Pneumatics: Purge delay: Void time: Temp: Makeup gas: Anode purge: Detector: Oven:


5890 Series II Helium, 20 cm/sec, 2.2 psi (80°C), 2.4 ml/min, constant flow HP-608, 30 m x 0.53 x 0.5 (Part No. 19095S-023) 1 µl Splitless mode 1 min 2.75 min 250°C Nitrogen, 65 ml/min 6 ml/min ECD, 320°C Temperature program listed above

2

10 5

3

5

6

7

4

89

10

19

12

15

20

14 13 16 18 17

15 Time (min)

20

Pub No.: 5963-2003

Nitrogen/Phosphorus Containing Pesticides: EPA Method 507

2

5 7 36 8

16. 17. 18. 19. 20. 21. 22.

Propazine Terbufos Pronamide Diazinon Disulfoton Terbacil Metribuzin

23. 24. 25. 26. 27. 28. 29.

Simetryn Alachlor Ametryn Prometryn Terbutryn Bromacil Metolachlor

Tricyclazole Merphos Carboxin Norflurazon Hexazinone Fenarimol Fluridone

20 19

40

27

30

37 36 35

11 21

28 29

15

10 Time (min)

42

38

22

18

205°C 178°C 4 min 30°C/min


2°C/min

GC: Column:

23

1

5

37. 38. 39. 40. 41. 42. 43.

80°C

24 25, 26 17

0

Triademefon MGK-264 Diphenamid Stirofos Butachlor Fenamiphos Napropamide

41

9 13 14 12 15 16

10

4

30. 31. 32. 33. 34. 35. 36.

Applications

EPA - 507 Targeted Compounds 9. Ethoprop 1. Dichlorvos 10. Cycloate 2. EPTC 11. Chlorpropham 3. Butylate 12. Atraton 4. Mevinphos 13. Simazine 5. Vernolate 14. Prometon 6. Pebulate 15. Atrazine 7. Tebuthiuron 8. Molinate

32 31

34 33

43 39

20

25

5890 Series II HP-5MS, 30 m x 0.25 mm x 0.25 µm (Part No. 19091S-433) Temperature: 260°C Volume injected: 1 µl Pneumatics: Splitless mode Purge delay: 1 min Carrier gas: Helium, 30 cm/sec (13.6 psi) pressure program Oven: Temperature program listed 1st ramp: 0.5 psi/min to 21 psi 2nd ramp: 1 psi/min to 31 psi Final temperature: 310°C for 4 min Detector: NPD, 290°C Makeup gas: Helium, 30 ml/min Hydrogen: 4.0 ml/min Pub No.: 5963-2003


439

Applications

Environmental


Chlordane and Toxaphene 300°C (1 min) 20°C/min

280°C (1 min) 190°C 80°C 1 min

Chlordane

0

10

3°C/min

30°C/min

15 Time (min)

Toxaphene

20

Carrier: Column:

25

0

10


Helium, 20 cm/sec 2.2 psi (80°C), 2.4 ml/min constant flow HP-608, 30 m x 0.53 mm x 0.5 µm (Part No. 19095S-023) Splitless, 1 µl, inlet 250°C Temperature program listed above ECD 330°C

Organo Chlorine Pesticides

Phenols

1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11.

Tetrachloro m-xylene a-BHC Lindane Heptachlor Endosulfan Dieldrin

275°C

150°C

20

EPA-625 Acid Extract 1. Phenol 2. 2-Chlorophenol 3. 2-Nitrophenol 4. 2,4-Dimethylphenol 5. 2,4-Dichlorophenol 6. 4-Chloro-m-cresol

7. 8. 9. 10. 11.

2,4,6-Trichlorophenol 2,4-Dinitrophenol 4-Nitrophenol 4,6-Dinitro-o-cresol Pentachlorophenol

40°C

1 78 9

5

4

Sample: Carrier: Column: Inlet: Oven: Detector:

440

1.5

2 Time (min)

5 ppb CLP A in iso-octane Helium 0.6 ml/min, constant flow HP-5, 10 m x 0.1 mm x 0.17 µm (Part No. 19091S-002) Split 10:1, 0.5 µl Temperature program listed above Micro ECD

2.5

3

9

3 5

1

30°C/min

11

2

0.5

200°C (2 min) 20°C/min

70°C (1 min)

6 4

25

Pub No.: 5091-7567

45°C/min

0.015 min

3 1 2

Endrin DDD DDT Methoxychlor Decachlorobiphenyl 10

15 Time (min)

10

7 8

3.5


8 min Helium Ultra 2 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.32 mm x 0.52 µm (Part No. 19091B-112) On-column, 1 µl Temperature program listed above FID

11

Environmental

Fungicides 1. 2. 3. 4. 5. 6. 7.

Organotin Compounds

Dihcloro metabolite of terrazole Terrazole Pentachlorobenzene Hexachlorobenzene Pentachloronitrobenzene Pentachloroaniline Pentachlorothioanisole

1. 2. 3. 4. 5. 6. 7.

260°C 5°C/min

35°C 1 min

7 6

4

Dimethylpentyltin Tetrabutyltin Methtyltripentyltin Tributylpentyltin Dibutylpentyltin Butlypentyltin Tetrapentyltin

260°C 50°C 1 min

5

Sn (303 nm)

3

2 3

1

5

15°C/min

4

6 7

2 1 8

7


12 min Hydrogen, 66 cm/sec HP-101 (Methyl Silicone Fluid), 25 m x 0.2 mm x 0.2 µm (Part No. 19091Y-102) 10 30 pg/component, 1 µl Temperature program listed above ECD Pub No.: 5953-1470

Organomercury Compounds


80°C 4 min

Helium, 100 kPa HP-1 (Cross-Linked Methyl Silicone), 25 m x 0.32 mm x 0.17 µm (Part No. 19091Z-012) Splitless, 1 µl Temperature program listed above AED 330°C Pub No.: 5091-1551E

60°C

5°C/min

Ph2Hg 38 pg

0

5°C/min

Bu4Sn Bu2SnPh Bu2SnPh2 BuSnPh2

Solvent Vent

MeHgPh 40 pg

10

20

Me2SnPh 10 pg 35 Time (min)

40

45


Helium, 0.75 ml/min constant flow HP-5 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.2 mm x 0.11 µm (Part No. 19091J-002) Splitless, inlet 300°C, 1 µl Temperature program listed above AED 300°C, Tin selective at 303.4 µm Pub No.: 43-5091-6499E

Carrier: Column : Injection: Oven: Detector:

10

40

50

BuSnPh2 78 pg Ph4Sn Oct2SnPh 35 pg

Bu4Sn 14 pg Bu2SnPh 17 pg

Solvent Vent 0

Carrier: Column :

30 BuSnPh2 35 pg

Harbor Sediment

30

Oct2SnPh

360°C

Standard Solution

254 µm

25

12

Organotin Compounds 300°C

20

11

20

Time (min)

30

40

Helium, 0.75 ml/min constant flow HP-5 (Cross-Linked 5% Phenyl Methyl Silicone), 25 m x 0.2 mm x 0.11 µm (Part No. 19091J-002) Splitless, 1 µl, inlet 300°C Temperature program listed above AED 300°C, Hg selective at 254 µm Pub No.: 5091-6500E


441

Applications

Carrier: Column:

10 9 Time (min)

Applications

Environmental


Volatiles Waste and Soil Analysis, GC/MS 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Pentafluorobenzene Dichlorodifluoromethane Chloromethane Vinyl chloride Bromomethane Chloroethane Trichlorofluoromethane 1,1-Dichloroethene Methylene chloride trans-1,2-Dichloroethene

11. 12. 13. 14. 15. 16. 17. 18. 19. 20.

1,1-Dichloroethane 2,2-Dichloropropane cis-1,2-Dichloroethene Bromochloromethane Chloroform Dibromofluoromethane 1,1,1-Trichloroethane 1,4-Difluorobenzene Carbon tetrachloride 1,1-Dichloropropene 40

21. Benzene 22. 1,2-Dichloroethane 23. Trichloroethene 24. 1,2-Dichloropropane 25. Dibromomethane 26. Bromodichloromethane 27. cis-1,3-Dichloropropene 28. Toluene-d8 29. Toluene 30. trans-1,3-Dichloropropene 52, 49, 41, 53 60, 42 58, 61 59 48 56 21, 45 39 55 57 12, 19, 22 29 44 47 16, 37 46 7 13 20 54 30 33, 31 25 63 65 5 1 23 34 14 17 32 64 66 4 8 9 10 15 35 18 24 2628 11 43 36 23 27 62

31. 32. 33. 34. 35. 36. 37. 38. 39. 40.

1,1,2-Trichloroethane 2-Bromo-1-chloropropane Tetrachloroethene 1,3-Dichloropropane Dibromochloromethane 1,2-Dibromoethane Chlorobenzene 1,1,1,2-Tetrachloroethane Ethylbenzene m,p-Xylene

Purge/trap parameters Line temperature: 120C Valve temperature: 120C MCS temperature: 120C Purge temperature/time: 30C (11 min) Dry purge time: 3 min MCS desorb temperature: 45C Desorb pre-heat:250C 8.00 24.00 Desorb 4.00 6.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 2.00 Time (min) temperature/time: 255C (2 min) Oven parameters Bake Sample 5 ml, 100 ppb calibration standard mix Initial temperature: 35C (4 min) temperature/time: 260C (8 min) Injection parameters Ramps 1st ramp: 8C/min to 180C MCS bake Temperature: 220C Final temperature: 180C (2.87 min) temperature: 310C Injection: P/T, split mode (split ratio 40:1) Detector parameters TPC pressure: 8.0 psi Carrier: Helium, 0.5 ml/min constant flow, 9.3 psi Transfer line: 280C capillary direct (35C) vacuum compensated Solvent delay: 0.9 min Column: HP-VOC, 30 m x 0.2 mm x 1.11 m (Part No. 19091R-303) Mass range: 45-450

57. o-Xylene 58. Styrene 59. Bromoform 60. 1,4-Dichlorobenzene-d4 61. Isopropylbenzene 62. 4-Bromofluorobenzene 63. Bromobenzene 64. 1,1,2,2-Tetrachloroethane 65. 1,2,3-Trichloropropane 66. n-Propylbenzene 41. 2-Chlorotoluene 42. 4-Chlorotoluene 43. 1,3,5-Trimethylbenzene 44. tert-Butylbenzene 45. 1,2,4-Trimethylbenzene 46. sec-Butylbenzene 47. 1,3-Dichlorobenzene 48. 4-Isopropyltoluene 49. 1,4-Dichlorobenzene 50. 1,2-Dichlorobenzene 51. n-Butylbenzene 52. 1,2-Dibromo-3-chloropropane 53. 1,2,4-Trichlorobenzene 54. Hexachlorobutadiene 55. Naphthalene 56. 1,2,3-Trichlorobenzene

Volatiles in Water (EPA Method 524.2) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.

Dichlorodifluoromethane Chloromethane Vinyl chloride Bromomethane Chloroethane Trichlorofluoromethane 1, 1-Dichloroethane Methylene chloride 1-1, 2-Dichloroethene 1, 1-Dichloroethane 2, 2-Dichloropropane c-1, 2-Dichloroethene Bromochloromethane Chloroform 1, 1, 1-Trichloroethane 1, 1-Dichloropropene

Column: Oven: Carrier: Injector: Detector: Vocarb 3000 Purge Flow:

17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32.

25 m x 0.2 mm x 1.12 m HP-624 (Part No. 19091V-402) 35 (4 min), 8°C/min to 180°C Helium, 7.3 psi, Constant Flow P&T, Split 30/1 MSD, 2505C Trap 6 40 ml/min 3 2 1 45 2

442

33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48.

Carbon Tetrachloride Benzene 1, 2-Dichloroethane Fluorobenzene Trichloroethene 1, 1-Dichloropropane Dibromomethane Bromodichloromethane c-1, 3-Dichloropropene Toluene t-1, 3-Dichloropropene 1, 1, 2-Trichloroethane Tetrachloroethen 1, 3-Dichloropropane Dibromochloromethane 1, 2-Dibromoethane

Chlorobenzene 1, 1, 1, 2-Tetrachloroethane Ethylbenzene m-Xylene p-Xylene o-Xylene Styrene Bromoform Isopropylbenzene 4-Bromofluorobenzene Bromobenzene 1, 1, 2, 2-Tetrachloroethane 1, 2, 3-Trichloropropane n-Proplybenzene 2-Chlorotoluene 4-Chlorotoluene

49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63.

1, 3, 5-Trimethylbenzene t-Butylbenzene 1, 2, 4-Trimethylbenzene s-Butylbenzene 1, 3-Dichlorobenzene 4-Isopropyltoluene 1, 4-Dichlorobenzene 1, 2-Dichlorobenzene-d4 1, 2-Dichlorobenzene n-Butylbenzene 1, 2-Dibromo-3-chloropropane 1, 2, 4-Trichlorobenzene Hexachlorobutadiene Naphthalene 1, 2, 3-Trichlorobenzene

36,37

11,12 7

9 8

4

10 6

16,17 18,19 14

33

26 29,30

21

15

35

34

13

20 8

22 24 28 25 27 31 23 32 10

12 14 Time (min)

47,48,49

P&T Parameters Line Temp: 120°C Valve Temp: 120°C MCS Temp: 100°C Purge Temp: 30°C (11 min) Dry Purge Time: 2 min MCS Desorb Temp: 40°C Desorb Preheat: 250°C Desorb Temp: 255°C (2 min) Bake Temp: 260°C (8 min) MCS Bake Temp: 180°C

52,53,54,55 41 46 51 43 50 44 45 56,57,58

38,39

61,62 60

59

40 42 16

18

20

22

63

Environmental

Pharmaceutical Solvents Extended List 1. 2. 3. 4. 5.

Methane Methanol (solvent) Acetonitrile (50 mg/ml) Methylene Chloride (500 mg/ml) Chloroform (50 mg/ml)

6. 7. 8. 9. 10.

Benzene (100 g/ml) 1,2-Dichloroethane (100 mg/ml) Trichloroethene (100 mg/ml) 1,4 Dioxane (100 mg/ml) Pyridine (100 mg/ml)

120°C 30°C 20 min

1

2

15°C/min 10

4 9 6 8

3

7 5

0

15

10

5

20

25

Time (min.)

GC: Sample: Column:

Carrier: Oven: Injector: Detector:

6890 50-500 ppm each component 60 m x 0.25 mm x 1.4 mm HP-624 (Part No. 19091V-436)

Hydrogen, 2.0 ml/min, Constant Flow 30°C (20 min), 15°C/min 120°C 200°C, 1 ml, 50/1Split 280°C, FID

Column: Carrier: Oven: Injector: Detector:

HP-624 25 m x 0.2 mm x 1.12 mm (Part No. 19091V-402) Helium, 32 cm/sec, constant flow Temperature listed above 1 ml, PTV, 220°C MSD, 250°C

Applications

Pharmaceutical Solvents

220°C 5 min 10°C/min 40°C 25°C/min 1 min 100°C

9

3

10

8

5 6

12

7

13

18

14

4 19

20

1 2 11 1

1. 2. 3. 4. 5.

2

3

Iso Octane Cyclohexane Benzene Pyridine Toluene

4

5

11. 12. 13. 14. 15.

6

7

2-methyl Pyridine Chlorobenzene Ethyl benzene Xylene(s) Styrene

8

16 15 17 10 9

33

34

35

36

37

38

39

40

41

Time (min.) 16. 17. 18. 19. 20.

Benzaldehyde 1,3-dichloro Benzene 1,4-dichloro 1,2-dichloro Benzene Phenyl Ethanol


21. 22. 23. 24. 25.

Thioanisole Dimethoxy Toluene Dimethylformamide Anisole Ethylene Glycol

443

Applications

Environmental


Semivolatiles Solid Waste Analysis, GC/MS 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22.

23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43.

1,4-Dichlorobenzene-d4 Pyridine N-Nitrosodimethylamine 2-Picoline Methyl methanesulfonate 2-Fluorophenol Ethyl methanesulfonate Phenol-d5 Phenol Aniline bis(2-Chloroethyl)ether 2-Chlorophenol 1,3-Dichlorobenzene 1,4-Dichlorobenzene Benzyl alcohol 1,2-Dichlorobenzene 2-Methylphenol bis(2-chloro-isopropyl)ether 4-Methylphenol Acetophenone n-Nitroso-di-n-propylamine Hexachloroethane

Nitrobenzene-d5 Nitrobenzene Naphthalene-d8 N-Nitrosopiperidine Isophorone 2-Nitrophenol 2,4-Dimethylphenol bis(2-Chloroethoxy)methane 2,4-Dichlorophenol Benzoic acid 1,2,4-Trichlorobenzene a-,a-Dimethylphenethylamine Naphthalene 4-Chloroaniline 2,6-Dichlorophenol Hexachlorobutadiene N-Nitroso-di-n-butylamine 4-Chloro-3-methylphenol 2-Methylnaphthalene 1,2,4,5-Tetrachlorobenzene Hexachlorocyclopentadiene

44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64.

Acenaphthene-d10 2,4,6-Trichlorophenol 2,4,5-Trichlorophenol 2-Fluorobiphenyl 2-Chloronaphthalene 1-Chloronaphthalene 2-Nitroaniline Dimethylphthalate Acenaphthylene 2,6-Dinitrotoluene 3-Nitroaniline Acenaphthene 2,4-Dinitrophenol Dibenzofuran Pentachlorobenzene 4-Nitrophenol 2,4-Dinitrotoluene 1-Napthylamine 2-Napthylamine 2,3,4,6-Tetrachlorophenol Fluorene

65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 81. 82. 83. 84. 85.

68 64, 66 34, 35 23, 24 21 25

19, 20 13, 14 8, 9 12 6

29 16

27

30

36, 41 37

33

42 43

58, 60 81 78, 80 82 83

51

74, 75

90

87 84

89

76

39 79

67 81

96 82

88

50

Chrysene-d12 Pyrene Terphenyl-d14 p-Dimethylaminoazobenzene Butylbenzyl phthalate Benzo[a]anthracene 3,3'-Dichlorobenzidine Chyrsene bis(2-Ethylhexyl)phthalate Perylene-d12 Di-n-octylphthalate Benzo[b]fluoranthene 7,12-Dimethylbenz(a) anthracene 99. Benzo (k) fluoranthene 100. Benzo[a]pyrene 101. 3-Methylcholanthrene 102. Dibenzo(a,j)acridine 103. Dibenz[a,h]anthracene 104. Indeno (1,2,3-cd) pyrene 105. Benzo[g,h,i]perylene

93, 94

52, 53 55

11

86. 87. 88. 89. 90. 91. 92. 93. 94. 95. 96. 97. 98.

65

62, 63 47

Diethylphthalate 4-Chlorophenyl-phenylether 4-Nitroaniline Diphenylamine n-Nitrosodiphenylamine Diphenylhydrazine 4,6-Dinitro-2-methylphenol Phenanthrene-d10 2,4,6-Tribromophenol 4-Bromophenyl-phenyl ether Phenacetin Hexachlorobenzene 4-Aminobiphenyl Pentachlorophenol Pentachloronitrobenzene Pronamide Phenanthrene Anthracene Di-n-butylphthalate Fluoranthene Benzidine

86

98, 99

91 92

85

100

102

72

7

3

95 2

4

5 4.00

6.00

8.00

12.00

10.00

14.00

Time (min.)

280°C 45°C 1 min

444

104

101

97

73

24°C/min


Conditions: Column: Carrier: Injection: Oven: Detector:

HP-5MS, 30 m x 0.25 mm x 0.25 m (Part No. 14091S-433) Helium, pressure program 0.1 psi (0.1 min) at 99 psi/min to 7.4 psi 0.1 l, 250°C Termperature program listed above MSD, 280°C

1600

103 105

Environmental

Organohalide Pesticides in Water, EPA Method 505 1. 2. 3. 4. 5. 6. 7. 8.

9. 10. 11. 12. 13. 14. 15. 16.

Hexachlorocyclopentadiene Hexachlorobenzene Simazine Atrazine Lindane Heptachlor Alachlor Adrin

Heptachlor epoxide δ-Chlordane α-Chlordane trans-Nonachlor Dieldrin Endrin cis-Nonachlor Methoxychlor

2 4 15 3

Injection parameters Temperature 250°C Volume Injected: 1 µl splitless Purge delay: 1 min Carrier gas: Helium, 2.0 m./min, constant flow, 42 cm/sec (22.4 psi at 80°C) Oven parameters Initial temperature Ramps 1st ramp: 2nd ramp: 3rd ramp: Final Temperature:

5

6

5

10

Column: Carrier: Injection: Detector:

Detector parameters ECD: 300°C Makeup gas: N2, 60 m./min Anode purge 6 ml/min

8 9

1

80°C (1 min) 30°C/min to 175°C (4 min) 6°C/min to 215°C (2 min) 15°C/min to 290°C 290°C (5 min)

10

7

Time (min.)

80°C 1 min

16

11 12 14 13




20

15

HP-5MS, 30 m x 0.25 mm x 0.25 m (Part No. 19091S-433) Helium, 42 cm/sec, constant flow 1 l splitless (1 min), 250°C ECD, 300°C

Sample 16 components EPA-505 targeted pesticides and 14 ppb Aroclor 1260 in hexane. Concentration of pesticides: 50 ppb each except 1.25 ppm for atrazine and simazine.

Pub No.: 5963-2003

EPA Method 625 Semivolatiles bis(2-chloroethyl)ether Dichlorobenzene, 1,3Dichlorobenzene 1,4Dichlorobenzene 1,2bis(2-chloroisopropyl) ether N-nitroso-di-n-propylamine Hexachloroethanene Nitrobenzene Isophorone

10. bis(2-chloroethoxy)methane 11. Trichlorobenzene 1,2,412. Napthalene 13. Hexachlorobutadiene 14. Hexachlorocyclopentadiene 15. 2-Chloronaphthalene 16. Dimethyl phthalate 17. Acenaphthylene 18. Dinitrotoluene 2, 617,18

19. 20. 21. 22. 23. 24. 25. 26. 27.

Acenaphthene Dinitrotoluene 2,4 diethyl phthalate Fluorene 4-chlorophenyl phenyl ether N-Nitrosodiphenylamine Azobenzene 4-Bromophenyl phenyl ether Hexachlorobenzene

12

2

4 5

1

11

7

3

8

23

15 19

22

14

9 10

80°C 4 min

26

21 25 24

28,45 29 27

30

6

35 36

31 32

Bis -2-ethylhexyl)phthalate Di-n-octyl phthalate Di-n-octyl phthalate Benzo (k) fluoranthene Benzo (a) fluoranthene Indeno (1,2,3-c,d) pyrene Dibenz (a,h) anthracene Benzo (g,h,i) perylene

Helium, 1ml/min constant flow HP-5 Trace Analysis 30 m x 0.25 mm x 0.25 µm (Part No. 19091M-443) Split/splitless Temperature program listed MS, 280°C


38 40

34

20

37. 38. 39. 40. 41. 42. 43. 44.

Column:

37

7°C/min 33

16

Anthracene Phenanthrene Di-n- butylphthalate Fluoranthene Pyrene ButylBenzyl phthalate Benz (a) anthracene Chrysene Dichlorobenzidine, 3,3Carrier:

310°C 5 min

13

28. 29. 30. 31. 32. 33. 34. 35. 36.

Applications

1. 2. 3. 4. 5. 6. 7. 8. 9.

39

41

42

43

44

0 12.00

14.00

16.00

18.00

20.00

22.00

Time (min.)

24.00

26.00


28.00

30.00 Pub No.: 5963-2003

445

Applications

Environmental


Nitrogen/Phosphorus Containing Pesticides, EPA Method 507 1. 2. 3. 4.

3

1

EPA Method 504

4

1. 1,2 Dibromoethane (EDB) 2. 1,2-Dibromo-3-chloropropane (DBCP)

Prometon Atrazine Prometryne Ametryn

200°C 1 min

40°C 4 min

2

1

10°C/min

2 0

10

30

20 Time (min)

40

Oven parameters Initial temperature 80°C Ramps 1st ramp: 30°C/min to 178°C (4 min) 2nd ramp: 2°C/min to 205°C 3rd ramp: 30°C/min to 310°C Final Temperature: 310°C for 4 min

Injection parameters Temperature 260°C Volume Injected: 1 µl Pneumatics: Splitless mode Purge delay: 1 min Carrier gas: Helium, 30 cm/sec measured at 80°C Pressure programming Initial head pressure: 13.6 psi 1st ramp: 0.5 psi/min to 21 psi 2nd ramp: 1 psi/min to 31 psi Void time: 1.63 min

0

2

GC: Sample: Carrier: Column:

Detector parameters Temperature: 290°C Makeup gas: Helium, 30 m./min Hydrogen: 4 ml/min

4

6

8

10 12 Time (min)

14

6890 10 ppb in hexane Helium 0.9 ml/min, constant flow HP-1MS, 30 m x 0.25 mm x 1.0 µm (Part No. 19091S-733) Splitless, 250°C, 1l, purge time 1 min Temperature program listed above ECD 300°C


16

18

Pub No.: 5965-7874

Pub No.: 5963-2003

Semivolatiles, EPA Method 624 1. 2. 3. 4. 5. 6. 7. 8. 9.

Bis (2-chloroethyl) ether Dichlorobenzene, 1,3Dichlorobenzene, 1,4Dichlorobenzene, 1,2Bis (2-chloroisopropyl) ether N-nitroso di-n-propylamine Hexachloroethanene Nitrobenzene Isophorone

10. 11. 12. 13. 14. 15. 16. 17. 18.

Bis (2-chloroethoxy) methane Trichlorobenzene, 1,2,4Naphthalene Hexachlorobutadiene Hexachlorocyclopentadiene 2-Chloronaphthalene Acenaphthylene 1Dimethyl phthalate Dinitroluene, 2,6-

19. 20. 21. 22. 23. 24. 25. 26. 27.

Acenaphthene Dinitrotoluene, 2,4Fluorene Diethyl phthalate 4-Chlorophenyl phenyl ether N-Nitrosodiphenylamine 2Azobenzene 4-Bromophenyl phenyl ether Hexachlorobenzene

28. 29. 30. 31. 32. 33. 34. 35. 36.

Phenanthrene Anthracene Di-n-butylphthalate Floranthene Pyrene Butylbenzyl phthalate Benz (a) anthracene Chrysene Dichlorobenzidine, 3,3'-

32 31

22 23 19

9

1112

6 10

13

15 16 21 24 27 14

5.00

446

34 30

33 36

35

38 40 41 37 39

25

17

80°C 4 min

44

GC: Carrier: Column: Injection:

20

Oven: Detector: 10.00

15.00 20.00 Time (min)

25.00

30.00

Bis(2-ethylhexyl) phthalate Di-n-octyl phthalate Benzo (b) fluoranthene Benzo (k) fluoranthene Benzo (a) fluoranthene Indeno (1,2,3-c,d) pyrene Dibenz (a,h) anthracene Benzo (g,h,i) perylene


43 42

26

18

3 7 2 48 1 5

28 29

37. 38. 39. 40. 41. 42. 43. 44.

6890 Helium, 33 cm/sec (1 ml/min) constant flow HP-5MS, 30 m x 0.25 mm x 0.25 µm (Part No. 19091S-433) On-column, 1 µl, inlet=83°C to 315°C (5 min) at 50°C/min Temperature program listed above MSD 280°C

Pub No.: 5963-2003

Environmental

Polychlorinated Biphenyls (PCBs) heptahexa-

octa-

penta-

tri-

320°C 5 min 10°C/min 80°C 30°C/min 2 min 200°C

tetranonadeca-

di-

tri-

8

10

12

GC: Sample: Carrier: Column: Inlet: Oven: Detector:

14

Time (min) 16

18

20

22

6890/microECD HP 7673, 10 µl syringe, 1 µl injection Helium, 16.8 psi, flow 1.4 ml/min HP-5MS, 30 m x 0.25 mm x 0.25 µm (Part No. 19091S-433) Splitless Temperature program listed above 330°C makeup gas: nitrogen, constant column and makeup flow

EPA Method 8061 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

11. 12. 13. 14. 15. 16. 17. 18. 19. 20.

Dimethyl phthalate Diethyl phthalate Di-isobutyl phthalate Di-n-butyl phthalate bis (4-Methyl-2-pentyl) phthalate bis (methoxyethyl) phthalate Di-amyl phthalate bis (2-Ethoxyexyl) phthalate Hexyl-2-ethylhexyl phthalate Di-n-hexyl phthalate

HP-608

17

3


6890 Helium, 20 cm/sec 2.1 psi constant flow HP-5 30 m x 0.53 mm x 0.5 µm 30 m x 0.53 mm x 0.88 µm (Part No. 19091J-023) HP-608 (Part No. 19095S-023) 260°C, 2 µl splitless pressure pulse split/less 1 min at 20 psi 60°C Dual ECD, 300°C

18

4 6

7

9 8

3

11 14 10

5°C/min

60°C 35°C/min 6.5 min

13

15

16 1

15 Time (min)

20

25

5

20

4 6

19 10

280°C 200°C

12

17

20

2

5

GC: Carrier: Columns:

HP-5

12

5

1

bis (2-Ethylhexyl) phthalate Butylbenzyl phthalate bis (2-n-Butoxyethyl) phthalate Dicyclohexyl methyl phthalate Di-n-octyl phthalate Di-nonyl phthalate Benzyl benzoate (IS) Di-phenyl phthalate (SS1) Di-phenyl isophthalate (SS2) Dibenzyl phthalate (SS3)

Applications

Semivolatiles Phthalate Esters

5

7 8

9

11

1013

2

10


14

15 Time (min)

18

15 19

20

16

25 Pub No.: 5963-2003

447

Applications

Petrochemical/Hydrocarbon Processing


Petrochemical/Hydrocarbon Processing C1-C5 Hydrocarbons 1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11. 12.

Methane Ethane Ethylene Propane Cyclopropane Propylene 2 6 7 1 4

Fast C1 to C10 hydrocarbon separation

iso-Butane n-Butane Propadiene Acetylene trans-2-Butene 1-Butene

13. 14. 15. 16. 17. 18.

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Isobutylene cis-2-Butene iso-Pentane n-Pentane 1,3-Butadiene Propyne

8

3

12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22.

Methane Ethane Ethylene Propane Cyclopropane Propylene iso-Butane n-Butane Propadiene Acetylene trans-2-Butene

Butene-1 isobutylene cis-2-Butene iso-Pentane n-Pentane 1,3-Butadiene Propyne trans-Pentene Pentene-1 iso-Pentane cis-2-Pentene

23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33.

Hexane Benzene Heptane i-Octane n-Octane Cyclohexene Toluene Nonane Ethylbenzene p-Xylene Decane 250°C 2 min

12

5

11

23

16

14

15

10 9


10 15 Time (min.) Helium, 50 cm/sec (100°C), 7 ml/min, constant flow HP-PLOT/Al2O3s" 50 m x 0.32 mm x 8 µm (Part No. 19095P-S15) Split (65:1) 5 µl, Inlet 250°C 100°C isothermal FID 250°C

20

Methane Ethane Ethylene Propane Cycloproprane Propylene iso-Butane n-Butane

9. 10. 11. 12. 13. 14. 15.

12 3 4

8

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

1.5 min 17 11 12 1416

9 10 5


448

32

5

3

4 Time (min)

5

6

7

HP-PLOT Al2O3 S", 30 m x 0.25 mm x 5 m (Part No. 19091P-S33) Temperature program listed above Hydrogen, 28psi @120C 250C, Split flow 120 ml/min FID 250C 5 l headspace gases

Methane Ethane Ethylene Propane Cyclopropane Propylene iso-Butane n-Butane Propadiene Acetylene trans-2-Butene Butene-1 4 7 8 17 2 1 15 16 11

13

Carrier: Column:

28-31

Extended Analysis (C1 to C10) n-Pentane 1,3-Butadiene trans-2-Pentene 2-Methyl-2-butene 1-Pentene cis-2-Pentene 180°C n-Hexane 2 min 100°C 10°C/min 5°C/min 35°C

6

0

26-27

33

2

Oven: Carrier: Inlet: Detector: Sample:

16. 17. 18. 19. 20. 21. 22.

Propadiene Acetylene trans-2-Butene 1-Butene Isobutylene cis-2-Butene iso-Pentane

7

1

Column:

Light Hydrocarbons 1. 2. 3. 4. 5. 6. 7. 8.

25

25°C/min

18

5

Carrier: Column:

24

11-16 17-22

17 0

0

120°C 1 min

1-10

13

10

15 Time (min)

6

20 21 19 18 20

Helium, 50 cm/sec (35°C), 7 ml/min, constant flow HP-PLOT/Al2O3 30 m x 0.53 mm x 15 µm (Part No. 19095P-M23) Split (65:1) 5 µl, Inlet 250°C Temperature program listed above FID 250°C

13

26 18 25 24

12

10 5 9

20 0 Carrier: Column: Injection: Oven: Detector:

10

Isolbutylene cis-2-Butene Cyclopentane iso-Pentane n-Pentane 1,3-Butadiene trans-2-Pentane 2-Methyl-2-butene 1-Pentene cis-2-Pentene TDB Cyclohexane

25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36.

2-Methyl-pentane n-Hexane Heptane Benzene iso-Octane n-Octane Toluene Nonpane Ethylbenzene p- and m-Xylene o-Xylene Decane 225°C

14

3

25

13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24.

180°C 100°C 5 min 40°C 7.5°C/min 2.5 min 23 30 31 22 28 29 34 21 27 20 35 33 19 32

20 min 15°C/min

10 min 12.5°C/min

36

30 50 40 Time (min) Helium, 50 cm/sec, (35°C), 7 ml/min constant flow HP-PLOT/Al2O3 50 m x 0.53 mm x 15 µm (Part No. 19095P-S25) Splitless, 5 µl, inlet temp at 250°C Temperature program listed above FID 250°C 20


Oxygenates in Hydrocarbon Sample 1. 2. 3. 4.

Ethanol 2-Propanone Dichloromethane Acetic acid, methyl ester

5. 6. 7. 8.

Aromatics in Benzene Calibration Mix of Aromatics in Benzne ASTM Method 4494

Diethyl ether Pentane Acetic acid, ethyl ester Hexane 250°C

1

1. 2. 3. 4. 5. 6.

8

15°C/min

150°C 2 min

Cyclohexane Nonane Benzene Toluene Undecane Ethylbenzene 1

7. 8. 9. 10. 11.

p-Xylene m-Xylene Cumene o-Xylene n-Propylbenzene 92°C (4.5 min)

3 60°C

4°C/min

1 min

5 2

220°C (5 mins) 20°C/min

11

6 4

2

5

6 78

9 10

7

4 3

GC: Column: Oven: Carrier: Detector: Butadiene concentration:

4 Time (min)

5

6


1000 ppm in methanol

Hydrocarbons C1-C5 Using an HP-PLOT Q Column 180°C 7.5°C/min

60°C 8 min C4s C3s C2s

6

10

12

14

0.1% in benzene Helium, 32 cm/sec, 19.9 psi (60°C), 2.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 60 m x 0.32 mm x 0.5 µm (Part No. 19091N-216) Split (100:1), 0.5 µl, inlet temp at 220°C Temperature program listed above FID 270°C

Purity of o-Xylene ASTM Method 3797 1. Hydrocarbon 2. Ethylbenzene 3. p-Xylene 4. Cumene 5. o-Xylene

isobutane n-butane Cyclopropane

8

Time (min) Sample: Carrier: Column:

6890 HP-PLOT Q (Part No. 19095P-QO4) Temperature program listed above Helium, 25 psig FID

C1

4

7

92°C (4.5 min) 60°C

220°C (1 min) 20°C/min

4°C/min

1 min 3

Applications

3

2

1

5

C5s 4 1

0

5

10

15

20

Time (min) 2 GC: Sample: Carrier: Column: Injection: Oven: Detector:

6890 17 HC, 5µl (C1-20%, C2 to C5-1% to 14%) Hydrogen, 8 ml/min @ 60°C HP-PLOT Q, 0.53 mm x 30 m x 40 µm (Part No. 19095P-Q04) 250°C Split ratio 12:1 Temperature program listed above FID 250°C

0 Sample: Carrier: Column: Pub No.: 5965-7872


5 10 15 20 Time (min) Neat, 98%+ Helium, 32 cm/sec, 19.9 psi (60°C), 2.5 ml/min constant flow HP-INNOWax (Cross-Linked PEG), 60 m x 0.32 mm x 0.5 µm (Part No. 19091N-216) Split (100:1), 0.5 µl, inlet 220°C Temperature program listed above FID 270°C


449

Applications



Impurities in Styrene: ASTM D5135 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Nonaromatics Ethylbenzene p-Xylene m-Xylene Isopropylbenzene o-Xylene n-Propylbenzene p/m-Ethyltoluene Styrene a-Methylstyrene (366 ppm) Phenylacetylene (58 ppm) 2 -Methylstyrene Benzaldehyde 4

Transformer Oil Gas 150°C

5 min


7

6 11

7

13

12

8 5

Methane Ethane Ethylene Propane Propylene Acetylene 1-Butene

1

1


2 3 6

3

2.5

5

10

5

0

2 min 10°C/min

80°C

9

1. 2. 3. 4. 5. 6. 7.

4

7.5 Time (min)

0

10

12.5

5890 Series II GC Helium 8.6 psi (28 cm/sec), constant flow, 1.5 ml/min HP-INNOWax 30 m x 0.32 mm x 0.5 µm (Part No. 19091N-213) Split (40:1), 0.5 µl Temperature program listed above Pub No.: 5964-2129 FID 270°C

Natural Gas

2

Sample:

4 Time (min)

6

8

Qualitative Test Sample Transformer Oil Gas (Part No. 5080-8750) Helium, 50 cm/sec, 100°C HP PLOT Al2O3s" 50 m x 0.53 mm x 15 µm (Part No. 19095P-S25) Splitless, 5 µl, 250°C 100°C, isothermal FID 250°C


Natural Gas (Hydrocarbon)

Ethane

Methane

60°C 2 min

1 min 30°C/min 23 1

Water

Air/CO

1. 2. 3. 4. 5. 6. 7. 8.

240°C

5 4

C3

Methane Ethane Propane iso-Butane n-Butane iso-Pentane n-Pentane n-Hexane 180°C

i-C4/n-C4 100°C 67

i-C5/n-C5 neo-C5

70th Run

C6

30°C/min

1.5 min

C7

8

1st Run 0

2

4 Time (min)

6

8

0

1

2

3 Time (min)

GC: Sample: Carrier: Column: Injection: Oven: Detector:

450

6890 0.25 cc natural gas sample Helium (8.6 ml/min @ 60°C) HP-PLOT Q, 30 m x 0.53 mm x 40 µm (Part No. 19095P-Q04) Split mode (100 ml/min) Temperature program listed above TCD 250°C Publication No.: 5965-7872


Natural gas (Part No. 5080-8756) Helium, 50 cm/sec (100°C), 6 ml/min HP-PLOT/Al2O3 15 m x 0.53 mm x 15 µm (Part No. 19095P-S21) Split (50:1), 5 µl, Inlet 250°C Temperature program listed above FID 250°C

4

5


Baseline Resolution of Air/CO, CO2, and Methane in a Natural Gas Sample Methane 240°C

CO2

Ethane

Air/CO

1 min 30°C/min

60°C 2 min

Natural Gas (Hydrocarbon) 1. 2. 3. 4. 5.

6. 7. 8. 9.

C1 C2 C3 IC4 NC4

IC5 NC5 C6 C7

123 45

180°C 100°C

30°C/min

1.5 min 6 7

Water C3

i-C4/n-C4 i-C5/n-C5 neo-C5

2


4 Time (min)

C6

6

C7 9

8

0

6890 0.25 cc natural gas sample, Methane, 80%+ Helium (8.6 ml/min @ 60°C) HP-PLOT Q, 30 m x 0.53 mm x 40 µm (Part No. 19095P-Q04) Split mode (12:1) Temperature program listed above TCD 250°C


Carrier: Column:

Publication No.: 5965-7872

Separation of Refinery Gas Sample

1

1. 2. 3. 4. 5. 6. 7. 8. 9.

2 3 5

10. 11. 12. 13. 14. 15. 16. 17.

Air/CO C1 CO2 Ethylene C2 H2O COS H2O Propylene

2

4

6 Time (min)

8

10

12

Helium, 1 ml/min PONA (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.5 µm (Part No. 19091S-001) Split (200 400:1) Temperature program listed above FID 250°C Pub No.: 5091-0691E


Refinery Gas

C3 MeOH i-C4 t-C4 n-C4 cis-C4 i-C5 n-C5

200°C 1 min

60°C

20°C/min

1. 2. 3. 4. 5. 6.

7. 8. 9. 10. 11. 12.

Methane Ethane Ethylene Propane Propylene iso-Butane 2

5 min

n-Butane trans-2-Butene 1-Butene cis-2 Butene iso-Pentane n-Pentane

Applications

H2S

0

8

6

13

4 14

11

7 12

4 6

10 7

8

9

GC: Sample: Carrier: Column: Inlet: Oven: Split flow: Detector:

2

4

6 Time (min)

8

6890 Refinery gas and others Helium p=9.0 psi @ 60°C HP-PLOT Q, 0.53 mm x 30 m x 40 µm (Part No. 19095P-Q04) 250°C Split mode 0.25 cc valve Temperature program listed above 100 ml/min TCD 250°C

10

8 3

16 17 0

0

9

1

10

5

2

11 4

12

6

8

12 10

12

Time (min) GC: Carrier: Column: Injection: Oven: Split: Detector:

6890 Helium 7 ml/min HP-PLOT/AI203 50 m x 0.53 mm x 15 µm (Part No. 19095P-S25) Split (100:1), inlet 250°C, 5 µl 100°C Isothermal 100 ml/min FID 250°C

Pub No.: 5965-7872


Pub No.: 5964-2129

451

Applications



Refinery Gas (Hydrocarbon) 1. 2. 3. 4. 5.

6. 7. 8. 9. 10.

C1 C2, C2= C3= C3 IC4 2

3

Oxygenates in Gasoline

NC4 1-Butene/t-2-butene cis-2-Butene 35°C IC5 3 min NC5 4

1. 2. 3. 4.


8

12

0

5

10 Time (min)

Column: 5

6

Time (min) Helium, 1 ml/min PONA (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.5 µm (Part No. 19091S-001) Split (200 400:1), 2 cc loop Temperature program listed above Pub No.: 5091-0691E FID 250°C


BTEX in Gasoline 1. 2. 3. 4. 5. 6. 7.

Pre-column: Injection: Oven: Detector:

5 7

4 6

20


452

10 15 Time (min)

20

Helium (A) 10.7 psi (60°C) constant flow (B) Pressure program: 9 psi (9.5 min) to 30 psi (4 min) at 99 psi/min HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.53 mm x 2.65 µm (Part No. 19095Z-123) 20% TCEP on Chromosorb PAW 80/100 56 cm x 1.6 mm x 0.32 mm Split (20:1), 3 µl 60°C isothermal Pub No.: 5091-4701E TCD 200°C

30

40

325°C 40°C/min

40°C 1.5 min

C9 5

1 2

4

C21 7

6

1

Time (min)

5

0

Reformate Gasoline

3

10

20

Methylene chloride


60°C

3

0

15

6

3 min

92 Octane Commercial Gasoline

2

7

Total Recoverable Petroleum Hydrocarbons (TPHs) Diesel Range

Benzene MIK (ISTD) Toluene Ethyl benzene p/m-Xylene o-Xylene C9, C10 plus nonaromatics

1

45

6 Valve Off

Carrier:

4

3 7 12

Valve On 1

Ethylene glycol dimethyl ether Benzene/1-methylcyclopentene TAME Heavy hydrocarbons (B) Pressure program 8

(A) Constant Flow

567 8 3 4 5

3

5. 6. 7. 8.

Ethanol MTBE Methanol ETBE

0

10

20

30

2

3

4

5

6

7

8

Time (min)

40

Time (min)

Helium, 10.7 psi constant flow HP-1 (Cross-Linked Methyl Silicone), 30 m x 0.53 mm x 2.65 µm (Part No. 19095Z-123) Split (17:1), 1 µl Temperature program listed above TCD 200°C Pub No.: 5091-5608E

GC: Carrier: Column: Injection: Oven:

6890 Helium 0.6 ml/min HP-5, 10 m x 100 mm x 0.17 µm (Part No. 19091S-002) Splitless, 0.1 µl Temperature program listed above Pub No.: 5965-6692


BTEX

Olefins to C5 1. 2. 3. 4. 5.

1

Methanol Benzene Toluene Ethyl benzene Xylenes

1. 2. 3. 4. 5.

250°C 15°C/min

150°C 2 min

Ethylene Propylene 1-Butene 1-Pentene 1-Hexene

250°C 50°C 2 min 5

1

4 3

2

2

15°C/min

3 4

5

20 0 1

2

3


4

5

6 7 Time (min)

8

9

10

10 12 Time (min)

6890 Helium, 25 psig HP-PLOT Q, 30 m x 0.53 mm 40 µm (Part No. 19095P-Q04) Oven: Temperature program listed above Detector: FID Analyte concentration:100 ppm v/v

Pub No.: 5965-7872

14

16

18 20

Pub No.: 5965-7872

Sulfur Compounds in Naphtha

1. Ethylene oxide 2. 2-Chloropropene

1. 2. 3. 4.

250°C 2

8

12

Ethylene Oxide Synthetic Standard

1

6


6890 Helium, 25 psig HP-PLOT Q, 30 m x 0.53 mm x 40 µm (Part No. 19095P-Q04) Temperature program listed above FID

Oven: Detector:

11

4

50°C

15°C/min

2 min

Thiophene Methyl thiophenes Ethyl and dimethyl thiophenes Benzothiophene

1

130°C 70°C 35°C (15 min)

2

3

15°C/min

8°C/min

Applications

0

2

4

20

0

2

4

6

8

10 12 Time (min)

14

16

18

42 min

20

GC: 6890 Carrier: Helium, 25 psi Column: HP-PLOT Q, 30 m x 0.53 mm x 40 µm (Part No. 19095P-Q04) Injection: Split (40:1), 1 µl liquid injection, Oven: Temperature program listed above Detector: FID Pub No.: 5965-7872 Analyte concentration: 2000 ppm v/v



Helium, 26 cm/sec PONA (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.5 µm (Part No. 19091S-001) Split (400:1), 3 µl Temperature program listed above Pub No.: 5954-7615 FPD

453

Applications



Sulfur Gases 1. 2. 3. 4. 5. 6.

Sulfur Compounds in Natural GasSynthetic Mixture

COS + SO Methyl mercaptan Ethyl mercaptan Isopropyl mercaptan n-Propyl mercaptan sec-Butyl mercaptan

1

1. 2. 3. 4. 5. 6. 7. 8. 9.

4

Hydrogen sulfide Methyl mercaptan Ethyl mercaptan Dimethyl sulfide Isopropyl mercaptan tert-Butyl mercaptan n-Propyl mercaptan Thiophene and sec-Butyl mercaptan Isobutyl mercaptan 1 234

11 5 7 8 9 12 10

10. 11. 12. 13. 14. 15. 16.

n-Butyl mercaptan tert-Amyl mercaptan iso-Amyl mercaptan n-Amyl mercaptan n-Hexyl mercaptan tert-Dibutyl disulfide n-Octyl mercaptan 300°C 7°C/min

35°C 10 min 16

13

5 3 14

2 6


5 min Helium, 26 cm/sec HP-1 (Cross-Linked Methyl Silicone), 10 m x 0.53 mm x 2.65 µm (Part No. 19095Z-121) Splitless, 0.5 ml (capillary injection port) 35°C FPD

C20 C22 C24 C26 C28

6. 7. 8. 9. 10.

30 min Helium HP-1 (Cross-Linked Methyl Silicone), 50 m x 0.2 mm x 0.5 µm (Part No. 19091Z-205) Split (100:1) 0.5 ml Temperature program listed above FPD Pub No.: 5953-1662


Petroleum Wax 1. 2. 3. 4. 5.

15

6

Polywax 500 320°C 10 min

C30 C32 C34 C36 C38

65°C

1. 2. 3. 4. 5. 6.

6°C/min

34 5 6 7 8

350°C 15 min

C20 C30 C40 C50 C60 C69

50°C

2

10°C/min

3

2 9 4

10

1

1 10


454

20

Time (min)

30

40

Helium, 22 psi at 65°C, constant flow mode HP-5 (Cross-Linked 5% Phenyl Methyl Silicone), 50 m x 0.32 mm x 0.17 µm (Part No. 19091J-015) On-column injection, 1 µl 68°C to 250°C at 15°C/min Temperature program listed above Pub No.: 5091-4209E FID

5 6 0 Carrier: Column: Injection: Oven: Detector:

45 min Helium, pressure program: 7 psi (1 min) to 18 psi (16 min) at 0.5 psi/min HP-1 (Cross-Linked Methyl Silicone), 15 m x 0.53 mm x 0.88 µm (Part No. 19095Z-023) 1 µl, on-column injection, oven track mode Temperature program listed above Pub No.: 5952-6971 FID 390°C


Sulfur Compounds in Hexane

SFE of Diesel Oil in Soil

1. Propyl mercaptan 2. Dimethyl disulfide 3. Diethyl disulfide

280°C

250°C

20°C/min

50°C 2 min

15°C/min

10°C 2 min

1 2

2

GC: Carrier: Column: Oven: Detector: Analyte concentration:

4

6

8 10 Time (min)

12

14

16

18

20

6890 10 psi Helium head pressure HP PLOT Q, 0.53 x 30 m x 40 µm (Part No. 19095P-Q04) Temperature program listed above FID

0

2

Carrier: Column:

50 ppm

Pub No.: 5965-7872

Reference Gas Oil

4

Helium 45 cm/sec (70°C), 6 ml/min constant flow HP-50+ (Cross-Linked 50% Phenyl Methyl Silicone), 30 m x 0.53 mm x 1.0 µm (Part No. 19095L-023) Splitless, 1 µl, inlet 280°C Temperature program listed above FID 300°C


35°C

NIST 1-K Kerosene, SRM 16160.0152 wt% Sulfur

S 0

15

20

15°C/min

C

S channel

10

335°C 10 min 40°C

10°C/min

C channel

5

12

SimDis of Kerosene 335°C 10 min

ASTM 2887

0

10

6 8 Time (min)

Applications

0

3

2

4

6 Time (min)

8

10

25

Time (min) Carrier: Column: Injection: Oven: Detector:

Helium, 5 psi, constant pressure mode with EPC HP-1 (Cross-Linked Methyl Silicone), 15 m x 0.53 mm x 0.88 µm (Part No. 19095Z-023) On-column injection, 0.2 µl neat, oven-track mode Temperature program listed above AED 350°C Pub No.: 5091-5303E


Helium, 22 psi HP-1 (Cross-Linked Methyl Silicone), 25 m x 0.32 mm x 0.17 µm (Part No. 19091Z-012) Split (36:1), 0.2 µl neat Temperature program listed above Pub No.: 5091-5303E AED 350°C


455

Applications



SimDis of Diesel Fuel

Natural Gas 335°C 10 min

0.010 wt % Sulfur XRF 40°C

15°C/min

1. 2. 3. 4. 5. 6. 7.

Methane Ethylene Propane Isobutane Butane Isopentane Pentane 2

1

C

3

S

4 5

0

2

8 10 Time (min)

6

4

12

14

6

16 0

2

4

7

8

6

10

12

14

Time (min) Carrier: Column:

Helium, 22 psi HP-1 (Cross-Linked Methyl Silicone), 25 m x 0.32 mm x 0.17 µm (Part No. 19091Z-012) Split (36:1), 0.2 µl neat Temperature program listed above AED 350°C Pub No.: 5091-5303E


Refinery Gas 1. 2. 3. 4. 5. 6. 7.


6890 Helium 23 cm/sec, constant pressure HP-1 60 m x 0.53 mm x 5 µm (Part No. 19095Z-626) 35 Isothermal Split (250:1), 0.5 cc FID

Oven: Injection: Detector:

Pub No.: 5964-2129E

Refinery Gas 8. 9. 10. 11. 12. 13.

Methane Ethylene Acetylene Ethane Propylene Propane Propadiene

iso-Pentane 1-Pentene n-Pentene trans-2-Pentene cis-2-Pentene 2-Methyl2-Butene

14. 15. 16. 17. 18. 19. 20.

1. 2. 3. 4. 5. 6.

Cyclopropane Isobutane Isobulylene 1,3-Butadiene n-Butane trans-2-Butene cis-2-Butene

7. 8. 9. 10. 11. 12.

Methane Ethane Ethylene Propane Propylene iso-Butane

9

n-Butane trans-2-Butene 1-Butene cis-2 Butene iso-Pentane n-Pentane

2

6

6

12 4 11 13 10

5 34 2 1

7

14

1 15

7

17 16

8

3 18

19

GC: Carrier: Column: Injection: Oven: Detector:

456

8

9

11 Time (min)

6890 Helium 23 cm/sec HP-1 100 m x 0.25 mm x 0.5 µm (Part No. 19091Z-530) Split (250:1), 500 µl 35°C isothermal FID

8 5

10 11

12

20 0

7

9

12

13 GC: Carrier: Column:

Pub No.: 5964-2129E


2

4

6 Time (min)

8

6890 Helium 7 ml/min HP-PLOT/AI203 50 m x 0.53 mm x 15 µm (Part No. 19095P-S25) Split (100:1), inlet 250°C, 5 µl 100°C FID 250°C

10

12

Pub No.: 5964-2129E


Butadiene and Aromatics 1. 2. 3. 4.

1,3-Butadiene Cyclopentene 1 Benzene Toluene

1,3-Butadiene 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

250°C

2 150°C 2 min

15°C/min

11. 12. 13. 14. 15. 16. 17. 18. 19.

Methane Ethane Ethylene Propane Cyclopropane Propylene iso-Butane n-Butane Propadiene Acetylene

trans-2-Butene 20. 2-Methyl-2-butene 1-Butene 21. Pentene-1 Iso-Butylene 22. cis-2-Pentene 180°C cis-2-Butene 23. n-Hexane Iso-Pentane 100°C 10°C/min n-Pentane 1, 3-Butadiene 35°C 5°C/min Propyne 2.5 min trans-2-Penten 13 17 7 8 e 4 12

4

2

3 1

0

1

2

3

4

5 6 Time (min)

7

8

9

6

14 16

3

10

10

9

5


6890 Helium, 25 psig HP-PLOT Q, 30 m x 0.53 mm x 40 µm (Part No. 19095P-Q04) Oven: Temperature program listed above Detector: FID Concentration: 200 ppm (v/v) Pub No.: 5964-2129E, 5965-7872

5

0 GC: Carrier: Column: Injection: Oven: Detector:

11

10

15 Time (min)

15

18 19 20 21 22 23 20

25

6890 Helium, 30 cm/sec (35°C), 4.6 ml/min constant flow HP-PLOT Al2O3, 50 m x 0.53 mm x 15 µm (Part No. 19095P-S25) Splitless, 5 µl; Inlet 250°C Temperature program listed above FID 250°C Pub No.: 5964-2129E

Propylene 180°C Methane

Ethane Ethylene Propane Cyclopropane Propylene iso-Butane n-Butane

9. 10. 11. 12. 13. 14. 15.

Propadiene Acetylene trans-2-Butene Butene-1 Iso-Butylene cis-2-Butene Iso-Pentane

16. 17. 18. 19. 20. 21. 22. 23.

n-Pentane 1, 3-Butadiene Propyne trans-2-Pentene 2-Methyl-2-butene Pentene-1 cis-2-Pentene n-Hexane

100°C 35°C 2.5 min 6

4


6890 Propylene 90% Helium, 28 cm/ sec (35°C), 4.0 ml/ min constant flow HP- PLOT/Al2O3, 50 m x 0. 53 mm x 15 µm (Part No. 19095P-S25) Splitless, 5 µl; inlet 250°C Temperature program listed above FID 250°C

5°C/min

7 17

8


10°C/min

Applications

1. 2. 3. 4. 5. 6. 7. 8.

11

12 14

2

16

13 15 1 10 5 0

5

10

9 15 Time (min)

20

19

20 21 22

18

23 25

30

Pub No.: 5964-2129E


457

Applications



Ethylene 1. 2. 3. 4. 5. 6. 7. 8.

9. 10. 11. 12. 13. 14. 15. 16.

Methane Ethane Ethylene Propane Cyclopropane Propylene iso-Butane n-Butane

17. 18. 19. 20. 21. 22. 23.

Propadiene Acetylene trans-2-Butene Butene-1 Iso-Butylene cis-2-Butene Iso-Pentane n-Pentane 1, 1 2

3

3-Butadiene Propyne trans-2-Pentene 2-Methyl-2-butene Pentene-1 cis-2-Pentene n-Hexane

GC: Sample: Carrier:

6890 Ethylene 98.4% Helium, 50 cm/ sec (35°C), 7 ml/ min constant flow HP- PLOT/Al2O3, 50 m x 0. 53 mm x 15 µm (Part No. 19095P-S25) Split (65:1), 5 µl; inlet 250°C Temperature program listed above FID 250°C


4

6

100°C

7 8 35°C 2 min

11

5°C/min

17

12 14 16

13 18 19

15

20 9

10

22

5 0

23

10

5

21

15

20

25

Time (min)

Permanent Gases

Pub No.: 5964-2129E

Noble Gases 1. 2. 3. 4. 5. 6.

4

Neon Argon Oxygen Nitrogen Methane Carbon monoxide

1. 2. 3. 4. 5. 6.

Neon Argon Oxygen Nitrogen Krypton Xenon

35°C 3 min


4 2

3

5 6 6

5 1 2

1 3

2 GC: Sample: Carrier: Column: Oven: Detector:

4 Time (min)

6

6890 250 ml, split (75:1) Helium, 2 µl/min HP-PLOT/MoleSieve, 30 m x 0.32 mm x 12 µm (Part No. 19091P-MS4) 40°C Isothermal TCD Pub No.: 5964-2129E

458

2 GC: Sample: Carrier: Column: Oven: Detector:

Time (min)

6

6890 250 µl, split (50:1) Helium, 4 ml/min HP-PLOT/MoleSieve, 30 m x 0.53 mm x 50 µm (Part No. 19095P-MS0) Temperature program listed above TCD Pub No.: 5964-2129E


Odor Additives Separation of Mercaptans and Other Sulfur Compounds on HP-Plot Q 1. 2. 3. 4. 5. 6.

Hydrogen Sulfide Carbonyl Sulfide Ethanelthiol iso-propyl mercaptan n-propyl mercaptan n-butyl mercaptan

1. 2. 3. 4.

240°C 2 min

105°C 1 min

Sulfur Odorants in Natural Gas

15°C/min

Hydrogen sulfide COS t-butyl mercaptan Methyl ethyl sulfide

250°C 30°C 1 min

10°C/min

1

5

3 4

3

2

4

6

1

2

6 Time (min)

4

8

10

6890 Hydrogen p=3.8 psi @ 60°C HP-PLOT Q, 0.53 mm x 30 m x 40 µm (Part No. 19095P-Q04) Inlet: 250°C Split mode 0.25 cc injection Oven: Temperature program listed above Split flow: 100 ml/min Detector: FID 250°C Concentrations: H2, COS:

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