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suppl figures - Semantic Scholar
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suppl figures - Semantic Scholar
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BZR1. BZR1-CFP. /WT. BZR1-CFP. /a1a3.
Ponceau
S e c. Hypocotyl (mm). D. MS BRZ d f g bri1-1. 16 bzr1-1D a1a3 bzr1-1D. Fig. S5. MS BRZ. Hypocotyl (mm).
a!
BR!
b!
BR!
BRI1!
BRI1!
BSKs!
BSKs!
?
?
BSU1(PP1)!
BSU1(PP1)! ?!
PP2A! BIN2(GSK3)!
BIN2(GSK3)! pBZR1/2!
pBZR1/2!
BZR1/2!
BZR1/2!
Gene expression!
Gene expression!
Fig. S1!
a
Protein name
Gene ID
BZR1! RCN1! PP2A A2! PP2A A3! PP2A B" # PP2A B" $* PP2A B" !! 14-3-3 "! BIN2*! AtSK12! AtSK31!
At1g75080! At1g25490! At3g25800! At1g13320! At3g26020! At3g09880! At1g13460! At5g10450! At4g18710! At3g05840! At3g61160!
Unique peptides sequence coverage! IP1 IP2 IP1 IP2! 18! 1! 2! 2! 2! 1! 1!
b
c
Fig. S2.
14! 1! 6! 3! 5! 1! 4! 4! 6! 1!
59%! 2.7%! 2.5%! 2.6%! 6.1%! 2.2%! 2.2%!
54.2%! 2%! 14%! 6.6%! 10.2%! 2.2%! 7.7%! 15.7%! 14.2%! 2.1%!
a
AD-BZR1 A1
A2
A3
B’!
B’"
C1
C2
C5
BD -AH +AH
b
AD-BZR2 B’! B’"
BD -AH +AH
Fig. S3.
!!!!!!WS
c!
B"%-OX!
Fold change
a!
bri1-5 !
WS
2
CPD
d! bri1-5
B"%-OX/bri1-5
!
OX
1 0
b!
bri1-5
DWF4
!!!!!!!!!!!!B" % OX / bri1-116!
e! BL
-
Col!
+P! - P! Stain!
Fig. S4!
+
bri1-116!
B"!-OX/! bin2-1! B"!-OX/! bin2-1!
-
+
-
+
-
+!
*
b"! ! *
* *
3! *
*
*
*
1! BL (nM) 0! ! 0.1! 1!
10! 100! 500 !
*
8!
*
4! 0!
f! 5!
4! 2!
10!
MS
3!
Col BRZ! c5 BRZ! Col! c5! *
2!
**
4!
MS
BRZ!
*
0!
DWF4!
CPD!
Ponceau S -
+
-
Col C5
* MS
BRZ
g!
1!
pBZR1! BZR1!
BL
BRZ!
16 14 12 10 8 6 4 2 0
**
BZR1-CFP! BZR1-CFP! /WT! /a1a3 !
h!
e
D
Col! b"! !
+!
Fig. S5.
bzr1-1D! bzr1-1D bri1-116! bzr1-1D bin2-1! bzr1-1D a1a3!
root (mm)! hypocotyl (mm)!
Col!
d
c
Hypocotyl (mm)
UBC!
b 12! 10 ! 8! 6! 4! 2! 0!
c5!
WT! bri1-116! bin2-1! a1a3!
Col! C5! UBC!
Hypocotyl (mm)!
Col b"#! B"# !
Fold change !
a
a!
c!
b! ATG!
pp2ab"$!
B’!
ATG!
pp2ab"#!
B’"
30 cycles! 60 cycles!
Fig. S6.
b!
a! 1!
214!
252!
-AH!
336!
△PEST3!
+AH! BD-B"#!
c!
BZR1-YFP-1! !P3-YFP-11! BZR1-YFP-5! !P3-YFP-15! BL
-
+
-
+
-
+
-
+!
pBZR1! BZR1!
!
p△P3-YFP !P3-YFP
!
!"#$%&
d! %P3-YFP-12
%P3-YFP-11
%P3-YFP-15
%P3-YFP-16 !
P△P3-YFP! Stain!
Fig. S7
PP2AB"#-GFP!
PP2AB"$-GFP!
PP2AB"%-GFP!
Fig. S8.
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