Primer Number Primer name Purpose Target region Sequence (5' to 3

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2. H77_ΔFse_SDMr SDM. H77 core. GGCCAGGAAGGTTCCCTGTTGC. 3. H77_E1E2_SCRf E1E2 Screening H77 core. GCGCAATTTGGGTAAGGTCA. 4.
Primer Number 1 2 3 4 5

Primer name

Purpose

H77_ΔFse_SDMf H77_ΔFse_SDMr H77_E1E2_SCRf H77_E1E2_SCRr G1E1E2>H77Fa

SDM SDM E1E2 Screening E1E2 Screening E1E2 In-Fusion

6

G1E1E2>H77Fb

E1E2 In-Fusion

Target region H77 E1E2 H77 core H77 core H77 NS2 H77 / 1a E1E2 Gt1 E1E2

7 8

G1E1E2>H77Ra G1E1E2>H77Rb

E1E2 In-Fusion E1E2 In-Fusion

Gt1 E1E2 Gt1 E1E2

9

G1E1E2>H77Rc

E1E2 In-Fusion

Gt1 E1E2

10

G1E1E2>H77Rd

E1E2 In-Fusion

Gt1 E1E2

11

G1E1E2>H77Re

E1E2 In-Fusion

Gt1 E1E2

12

G1E1E2>H77Rf

E1E2 In-Fusion

Gt1 E1E2

13 14 15 16 17 18 19 20 21 22

G1E1E2>H77Rg J6_ΔFse_SDMf J6_ΔFse_SDMr J6_E1E2_SCRf J6_E1E2_SCRr G2E1E2>J6Fa G2E1E2>J6Fb G2E1E2>J6Fc G2E1E2>J6Ra G2E1E2>J6Rb

E1E2 In-Fusion SDM SDM Screening Screening E1E2 In-Fusion E1E2 In-Fusion E1E2 In-Fusion E1E2 In-Fusion E1E2 In-Fusion

23 24 25

SDM SDM Screening Screening

27

J6_ΔAfeI_SDMf J6_ΔAfeI_SDMr JFH_CNS2_SCRf JFH_CNS2_SCRr J6_C-NS2>JFHf

Gt1 E1E2 J6 E1E2 J6 core J6 core J6 NS2 Gt2 E1E2 J6 E1E2 JFH-1 E1E2 Gt2 E1E2 J6 / JFH-1 E1E2 J6 Core J6 NS2 JFH-1 5’UTR JFH-1 NS3

28

J6_C-NS2>JFHr

29 30 31 32 33

G3_E1E2_SCRf G3_E1E2_SCRr G3E1E2>G3Fa G3E1E2>G3Fb G3E1E2>G3R

CoreNS2 InFusion CoreNS2 InFusion Screening Screening E1E2 In-Fusion E1E2 In-Fusion E1E2 In-Fusion

J6 CoreNS2 J6 CoreNS2 Gt3 E1E2 Gt3 E1E2 Gt3 E1E2 Gt3 E1E2 Gt3 E1E2

26

Sequence (5’ to 3’) GGCCAAGCGGAGGCGGCTTT GGCCAGGAAGGTTCCCTGTTGC GCGCAATTTGGGTAAGGTCA ACCCTTCAGATACCACGCAA AGGGAACCTTCCTGGTTGCTCTTTCTCTATC AGGGAACCTTCCTGGTTGCTCTTTCTCTATCTTCCT TC GCCGCCTCCGCTTGGGATATGAGTAACATCATCCA GCCGCCTCCGCTTGGGCTATGAGTAACACCATCC A GCCGCCTCCGCTTGGGCTACCAGCAGCATCATCC A GCCGCCTCCGCTTGGGCTATCAGCAGCATCATCC A GCCGCCTCCGCTTGGGCTATGAGTAGCATCATCC A GCCGCCTCCGCTTGGGATATGAGTAGCATCATCC A GCCGCCTCCGCTTGGGCTATGAGTAACATCATCCA CCGGCCGAAGCAGCACTA CCGGGTAAGTTCCCTGTTGC GCTTTGCCGACCTCATG GTACCAAGCAGCCACGAAAA AGGGAACTTACCCGGTTGCTCTTTTTCTATC AGGGAACTTACCCGGTTGCTCCTTTTCTATC AGGGAACTTACCCGGTTTCCCCTTTTCTATC CTAGTGCTGCTTCGGCTTGGCCCA CTAGTGCTGCTTCGGCCTGGCCCA GCTCCCATCACTGCTTATGCCCAGCAAACAC GCTCATGGTGCACGGTCT ACTCTATGCCCGGCCATTT GGCCTGTTCTGTCCTGTCA CCGTGCACCATGAGCACAAATCCTAAACCTCAAAG AA AGCAGTGATGGGAGCGAGAAGACTCCACCCCTT CGTAGGAGGCGTCGCAAG AGCCAATACCATGTGTCCCA AGGGAACTTGCCCGGTTGCTCCTTTTCTATCTTCC AGGGAACTTGCCCGGTTGCTCTTTCTCTATCTTC AGCATCAGCCAAAGGGCAACGCACACGC

Supplementary table 1: Primers for PCR amplification. Primer numbers, names, target gene, breadth of target and sequence are detailed. HCV genotype is abbreviated to ‘Gt’ and 15 base 5’ homology tags are indicated in grey.

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