Qrr2 was present at 1.4 µM in each case. On the right, the upper panels show a ribbon representation of Qrr2, predicted by iFoldRNA (26,27), superimposed with.
Reverse primer (5' to 3'). Enzyme. Orientation. ~2 kb enhancer. GFP. GCCGGCTAGCACCGACACAGCTGTA. CTTGG. GCCGGCTAGCAGAGTCCTGATGG.
Primer Number Primer name Purpose Target region Sequence (5' to 3
Sequence (5’ to 3’) GGCCAAGCGGAGGCGGCTTT GGCCAGGAAGGTTCCCTGTTGC GCGCAATTTGGGTAAGGTCA ACCCTTCAGATACCACGCAA AGGGAACCTTCCTGGTTGCTCTTTCTCTATC AGGGAACCTTCCTGGTTGCTCTTTCTCTATCTTCCT TC GCCGCCTCCGCTTGGGATATGAGTAACATCATCCA GCCGCCTCCGCTTGGGCTATGAGTAACACCATCC A GCCGCCTCCGCTTGGGCTACCAGCAGCATCATCC A GCCGCCTCCGCTTGGGCTATCAGCAGCATCATCC A GCCGCCTCCGCTTGGGCTATGAGTAGCATCATCC A GCCGCCTCCGCTTGGGATATGAGTAGCATCATCC A GCCGCCTCCGCTTGGGCTATGAGTAACATCATCCA CCGGCCGAAGCAGCACTA CCGGGTAAGTTCCCTGTTGC GCTTTGCCGACCTCATG GTACCAAGCAGCCACGAAAA AGGGAACTTACCCGGTTGCTCTTTTTCTATC AGGGAACTTACCCGGTTGCTCCTTTTCTATC AGGGAACTTACCCGGTTTCCCCTTTTCTATC CTAGTGCTGCTTCGGCTTGGCCCA CTAGTGCTGCTTCGGCCTGGCCCA GCTCCCATCACTGCTTATGCCCAGCAAACAC GCTCATGGTGCACGGTCT ACTCTATGCCCGGCCATTT GGCCTGTTCTGTCCTGTCA CCGTGCACCATGAGCACAAATCCTAAACCTCAAAG AA AGCAGTGATGGGAGCGAGAAGACTCCACCCCTT CGTAGGAGGCGTCGCAAG AGCCAATACCATGTGTCCCA AGGGAACTTGCCCGGTTGCTCCTTTTCTATCTTCC AGGGAACTTGCCCGGTTGCTCTTTCTCTATCTTC AGCATCAGCCAAAGGGCAACGCACACGC
Supplementary table 1: Primers for PCR amplification. Primer numbers, names, target gene, breadth of target and sequence are detailed. HCV genotype is abbreviated to ‘Gt’ and 15 base 5’ homology tags are indicated in grey.