Construct Forward primer (5' to 3') Reverse primer (5' to 3 ... - PLOS

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Comment
Reverse primer (5' to 3'). Enzyme. Orientation. ~2 kb enhancer. GFP. GCCGGCTAGCACCGACACAGCTGTA. CTTGG. GCCGGCTAGCAGAGTCCTGATGG.
Construct

Forward primer (5’ to 3’)

Reverse primer (5’ to 3’)

~2 kb enhancer GFP ~1.3 kb enhancer GFP 511 bp enhancer GFP 511 bp enhancer mCherry

GCCGGCTAGCACCGACACAGCTGTA CTTGG GCCGGCTAGCGAGAGCATCCGTCTT GTGGG GCCGGCTAGCGTGTGTGCGCGGTG GAAAATG GCCGGCTAGCGTGTGTGCGCGGTG GAAAATG

GCCGGCTAGCAGAGTCCTGATGG CCTCTCC GCCGGCTAGCAGAGTCCTGATGG CCTCTCC GCCGGCTAGCAGAGTCCTGATGG CCTCTCC GCCGGGATCCAGAGTCCTGATGG CCTCTCC

Enzyme

Orientation

NheI

Minus

NheI

Minus

NheI NheI and BamHI

Minus Plus