Primer Sequence - PLOS

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Reverse primer sequence

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(in bp) Forward primer Reverse primer - PLOS

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Primer Sequence - PLOS

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Page 1.

Primer

Sequence

huRB1-SacI

5’ccc ggg gagctc tgaggatctcaggaccttggtgg

huRB1-miR144MulI

5’ggg ccc acgcgt gc aaaa tttt ata aaac aag cac

huRB1-miR144MulI-mut

5’ggg ccc acgcgt gc aaaa tttt ata aaac aag cac tgtca atatc

huRB1-miR199a*MulI

5’ggg ccc acgcgt ca ttct gagg tat gaaa tct gttt

huRB1-miR199a*MulI-mut

5’ggg ccc acgcgt ca ttct gagg tat gaaa tct gttt gtcatc tat ga

huPTEN-SacI

5’ccc ggg gagctc tgaatttttttttatcaagagggat

huPTEN-miR144MulI

5’ggg ccc acgcgt gtg caaa gggg tagg at gtg aac

huPTEN-miR144MulI-mut

5’ggg ccc acgcgt gtg caaa gggg tagg at gtg aac gtcat tat cac

huPTEN-miR136MulI

5’ggg ccc acgcgt aatta ttt ccttt ct gag ca ttcc

huPTEN-miR136MulI-mut

5’ggg ccc acgcgt aatta ttt ccttt ct gag ca ttcc gaggt tt ccc

miR-199a* Realtime primer

5’ tacagtagtctgcacattggtt

miR-17-5p Realtime primer

5’caaagtgcttacagtgcag

miR-328 Realtime primer

5’ ctggccctctctgcccttccgt

Human U6 primer forward

5’ gtgctcgcttcggcagcacatatac

Human U6 primer reverse

5’ aa aaa tat gg aa cgc ttc acga atttg

mo-Gapdh1F

5’ atg gtg aag gtc tgt gtg atc atc

mo-Gapdh250R

5’ tgg gtt ctc act cct gga aga agg