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List of primers. Primer. Sequence 5' to 3'. Position to. TSS. Reference. Antp-P1. CGTGGGCTAAGTCAACGGAA. 0. AAGGAGTTCTCGCTGGGATT. Antp-P1 +0.1K.

S4 Table. List of primers. Primer Antp-P1 Antp-P1 +0.1K Antp-P1 +1K Antp-P2 Antp-P2 +0.1K Antp-P2 +1K Antp-3’ Scr-P1 Scr-P2 Scr-3’ bxd-1 bxd-2 Ubx-P Ubx +0.1K Ubx +1K bx Ubx-3’

Sequence 5’ to 3’ CGTGGGCTAAGTCAACGGAA AAGGAGTTCTCGCTGGGATT AGAGCCCAGCCCCAAGATA CAACAAAGCACTCGAGGACC CAACGTTTTCTAGTCCCCCG AAAGTTTGCCCGCAGTGG AAGCAAAGTGCGAATCGAGC TCACTGGCGTTCAGTTGTGA CTGGGAATGAACCGAACGGA GACGTGCCAAATAGACGTGC TGTGTGGAAACGATGCGAATG AACGAGTGTAAAGCCGAGCA CTCGCCCTTCGTCTTGTTCT GAAGGCAGACATACACCCGG ACTTCGCGGATTGCTACGC AAGGCAACGCACGAGAGG ACACGCGGAACAGCTGAGT GTATGAGTGAGCCAGAGCGCT CGAACTGCGACGGATGGATA GAAGGAGCACAAGATGGCCT TAGTCTTATCTGTATCTCGCTCTTA CAGAACCAAAGTGCCGATAACTC GCACGCACTAAACCCCATAA TCCACCTCCTCTTCCTCTCTC GCGCTCTCTCTTGAGTGTTCGT GGCGAGCGCATTTTCCTT TCCAATCCGTTGCCATCGAACGAAT TTAGGCCGAGTCGAGTGAGTTGAGT AATTGGTTTCCAGGGATCTGC ATCCAAAGGAGGCAAAGGAAC CCATAAGAAATGCCACTTTGC CTCTCACTCTCTCACTGTGAT AGTTCCACACGAATCATTATCTGAC CTTCATTCGCCGGTTCTGGA

Primers used for RT-qPCR GCTCACTTCTACCAGACTGGC Ubx CTTCATTCGCCGGTTCTGGA CCGTGGATGAAGCGAGTACA Scr ACCCATGTGGTAGGGTACGA ATGCCGTCTCCACTGTATCC Antp CGACGGGTCAAGTAGCGATT ACTTCATCCGCCACCAGTCGG Rpl32 CGCTCGACAATCTCCTTGCGC

Position to TSS

Reference

0 +0.1 kb +1 kb 0 kb +0.1 kb +1 kb +100.5 kb 0 kb 0 kb +24 kb -29.6 kb [1] -28.9 kb 0 kb

[2]

+0.1 kb +1 kb +31.7 kb +74 kb

[1]

References 1.

Gambetta MC, Oktaba K, Muller J. Essential role of the glycosyltransferase sxc/Ogt in polycomb repression. Science. 2009;325(5936):93-6. doi: 10.1126/science.1169727 PMID: 19478141

2.

Chopra VS, Hong JW, Levine M. Regulation of Hox gene activity by transcriptional elongation in Drosophila. Curr Biol. 2009;19(8):688-93. doi: 10.1016/j.cub.2009.02.055 PMID: 19345103