Table S1. List of primers used in this study Restriction ...

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Table S1 List of primers used in the study

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Table S1. Primers used in the study. Name Sequence

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Table S5. Primers Used in this Study. Primer Number Sequence

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Supplementary Table 1. qPCR primers used in this study

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Table S1. List of primers used in this study Restriction ...

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family transcriptional regulator RokA of Streptococcus pneumoniae D39. Microbiology 158, 2917â2926. Table S1. List of primers used in this study. Restriction ...

Table S1. List of primers used in this study Restriction sites are underlined. Name PSPD0424-F PSPD0424-R PSPD0426-F PSPD0426-R PSPD0424-F1 PSPD0424-R1 PSPD0424-F2 PSPD0424-R2 PSPD1830-F PSPD1830-R SPD0423-1 SPD0423-2 SPD0423-3 SPD0423-4 Spec-F Spec-R SPD0423-nco SPD0423Cstrep-hin

Nucleotide sequence (5′ to 3′)

Restriction site CATGGAATTCGTTGCTGGATTTCGATATAG EcoRI CATGGGATCCGACTTTTGTTTCTAAAAACGC BamHI CATGGAATTCGGATGTTTATCGTGTAATTG EcoRI CATGGGATCCGACAATTGTAAAACCAAG BamHI CATGGAATTCAAGGCTGATTGTTTTGTAATC EcoRI CATGGGATCCTTCATCACCGGAACTCC BamHI CATGGAATTCTTAAACATAAGAAAAAATC EcoRI CATGGGATCCAATATACCACTAACAGAAAG BamHI CGGGATCCCCGCTAGAAGCTGCTCCCCACC EcoRI CGGAATTCCTTTTCACGAATCTCATTGT BamHI GAAGACTAGGAATGCCAAAACACTGAC – GCATGCGGCCGCCCTTAATATATTAGAAATCTATAGACCTG NotI TGCTAGGCGCGCCTGTAATCATTTGTTTCCTCC AscI CAACTCCTGCCGCTTCAAGACCT – GCATAGGCGCGCCCTAATCAAAATAGTGAGGAGGATAT AscI CGATTGCGGCCGCACTAAACGAAATAAACGCTAAAACG NotI CGATCCATGGTAAAGGAGGAAACAAATGATTAC NcoI GATCAAGCTTATTTTTCAAATTGTGGATGGCTCCAAGCGCTA HindIII GGTGAAATAATATCTTG

Table S2. List of ROK family proteins present in the genome of S. pneumoniae D39 wild-type D39 locus tag SPD0423 SPD0580 SPD1488 SPD1970

TIGR4 locus tag SP0473 SP0668 SP1675 SP2142

TIGR4 locus tag spr0420 spr0584 spr1519 spr1950

Description ROK family protein (RokA) ROK family protein (RokB) ROK family protein (RokC) ROK family protein (RokD)

Residues (aa) 407 319 294 289

S. Shafeeq, T. G. Kloosterman, V. Rajendran & O. P. Kuipers (2012). Characterization of the ROKfamily transcriptional regulator RokA of Streptococcus pneumoniae D39. Microbiology 158, 2917–2926.