Primer pair Forward primer and reverse primer

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Primer pair Forward primer and reverse primer

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... concentration (mM)/Thermal cycler (TG= TGradient thermal cycler - Biometra, GÃ¶ttingen, Germany; PT= PT-100 thermal cycler - MJ. Research, Watertown, MA ...

Additional file 1 - Primer sequences, PCR conditions for MC1R sequencing and PCR-RFLP analyses

Primer pair

Forward primer and reverse primer (5'-3')

ch5

aaacgatggtcagggagtg accgcactgtggcttctc aagatgccaaggaaggctc ctgacgctcaccagcaagt agtgcctggaggtgtccatcc ctgacgctcaccagcaagt

ch7 2-ch7

ch9 2-4K ch10 4K-E1 E1-2

E1-3 A-2

ch8

1

Use Fragment PCR 1 length conditions (bp) 597 63/2.5/TG Sequencing 592

63/2.0/TG

Sequencing

169

62/2.0/TG

agccatgagttgagcaggac caggacaccagcctccag agtgcctggaggtgtccatcc gcctgggtggccaggaca gtgagcgtcagcaacgtg acatagaggacggccatcag caagaaccgcaacctgcact cagcaggatggtgagggtgg ctcgttggcctcttcatagc gaagttcttgaagatgcagcc

376

62/1.5/TG

PCR-RFLP analysis with HaeIII (c.183C>T and c.242C>T) Sequencing

229

62/2.0/PT

Sequencing

365

59/2.0/TG

Sequencing

550

65/2.0/PT

Sequencing

267

62/2.0/PT

ctcgttggcctcttcatagc tgctggtcatgaagtccttg gctgccaccctcaccatcctgctgggcgac gaagttcttgaagatgcagcc

685

60/2.0/PT

Sequencing; PCRRFLP analysis with XbaI (c.673C>T) Sequencing

123

64/2.0/PT

accccctcatctatgccttc aaagatgggaaggaggttgc

586

65/2.0/PT

PCR-RFLP analysis with TaiI (c.748T>G) and HaeIII (c.801C>G) Sequencing

PCR conditions describe annealing temperature/MgCl2 concentration (mM)/Thermal cycler (TG= TGradient thermal cycler - Biometra, Göttingen, Germany; PT= PT-100 thermal cycler - MJ Research, Watertown, MA, USA).