Additional File A2: Phylogeny and multiple sequence

Additional File A2: Phylogeny and multiple sequence alignment of endopterygotan insects full length RD protein sequences.

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MHLHLLAAEKTPPSPSPNPTPTPSASPSGHTGAAGESQLDLASQTESQLQLGLPLASGYG MHL---------PVS-----LSSCTVS--------------------------------MHL---------PSGGPTAIIPPISNNEANVTPTASTEWDIYKDEE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MHL---------PVG-----SVSCSGESFGSLSQQHHHHQHHQQHHQHHQQHHQLQ-------------------------------------------------------------------------------------------------------------------------MKS---------AVPRPPRITTDCRGSLWAIYGDGDDRQADIRKPDSGGSSATLLEYPGH ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

1 1 1 1 61 14 38 1 1 1 1 1 1 1 1 1 1 43 1 1 52 1 1 1 1

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LGLGLGLGLGLGLGQELVADHSTTVAPVSVAGPGRLGRSINGSGGSHHHHHLHHHYSPYH ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QEQDNGNLVALQP-----------------------------------------------------------------------------------------------------------------------------------------------------------PSCEPEAEKQLLEKKLLVAAIAE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

1 1 1 1 121 14 38 1 1 1 1 1 1 1 1 1 1 56 1 1 75 1 1 1 1

------------------MDIFGRCNGGGGG--------------SSTVSGSG--AGSVR ---------MTNPARDARGGVAVENYAGGGSDPSSRLQQSHHQQHLPHHSSHHHHHHHHH ----------------------MHISAEVSSTTSNQIQQQQQQHQQQQQHQLLQHQQQQT -----------------------------------------------------------HAHPYHPPHPHAPHHHHHHHPPYPYPPAGPHPPAMVTSSSTSPTGNGWSSSTGDFKGITA -------------------------EEAWPKSSGMCEP-------------------------------------------MLEPPAG---------------------------------------------------------------------------------------------------------------MHLAT----------------------------PEVTS ---------------------------------------------------------------------------------MHLAS-----------------------------EVSS ----------------------------------------------------------------------------------------------------------------------------------------MDIFGRCNGGAGLGVNSVPATAAVVAQSATTRSANNLAGPVV ----------------------MGQSESCG-----------------------PPPQHHS ----------------------------------------------------------------------------------------------------------------------------------------AQYQQQIQEQHQQQQQQVEG-----------------------------------------------------------------------------------------MTNPLRDAR----PEDRPLGLDYPDPRLVRNGHQEHQGNTTAHQ-----------------------ESPWSVQIEEASEITTSCPEGTCLGDEGRQLADGMHLPVGSVG ------------------------------------------------------------------------------------------------------------------------------------------------MHLPAGPTMVANNTQVLAAAAAAAAAAAAAVAQG -------------------------MEHEGANSNNYKMHLTTTSSNSTASNANNNNNNTA

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

27 52 39 1 181 27 45 1 11 1 10 1 1 43 16 1 1 76 1 41 118 1 1 35 36

GSGNESSCGTASG-----------ASESGGTSDRMQLTGASAPGVAASPESGVSP----QQQQHQHRHQQDQQHSSSGSNRLRRSRTSSLRRNGSRTMHLSGETQAQQQAAAPA----ATTTTTKRRNAESSASSNNNNNNNTSTTNNNNTNNNNNSTTNNNNNNNNNNVKTK--------------------------------------------------------MHL----VATATGGGVGGATQGATASTGATAAEVLAVSSSASVGSSSPTGGASNGTAHSGHSGHTGG -----------------------------------------SSGYVS-------------------------------------------ANNNAENSSPSPGGSG----------------------------------------------MRFGLKGGAAVVT---------------TTNPTSMELNGNSGGGLNTDSVNT---NGTTNDGTSKHQP--------------------------------------------------------------------MHL------------------------TTNGLGHEAPSSSYNGGSLPSQP--------------------------------------------------------------------------------------------------------------------------------------GPEHASTVGNTGGGGNGNNNGPAAPAEAATSQSRMQLTGASAPGNAASPESGVSP----RDFSSHFRAVRWPRHAAKKLTSMHLATGEVTSTTGHYDRSYGN-------------------------------------------------------------------------------------------------------------------------------MHL--------------------------------QRQEEAAEPGPTGAAGEMS------------------------------------------------MHLTGANSGAVSPDTSSTL---------------------IGAARIRRTRTSSTRRR----MHLANEVPPQGSTGG------CGENYATVGSHEGAGPCGPSLAPLQGVNTRYQQADDADAGGGGGEMS-------------------------MHITDVNVDHLLSSTAPLANHPSKDPVRRNNLHNSYKMAEGG--------------------------------------------------------MHL----PGPQQSSNATTASAIAINPAQSLANTSTHSASSTGSSTPDLSTNNTSSSSNATTS----NNNNTSSNNNTTN--------TNGSSNNNTSGNNNNSSGNNSNTEQNTPPTPAQL-----

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

71 107 94 4 241 33 61 14 48 4 33 1 1 98 59 1 4 95 20 73 165 43 4 90 83

------------------LTDAYTKMTSDILAERTLGDFVSEHPGELVRTGSPHLVCTVL ------------------SQLPDYALTADLLAERTLDGLLAEHPGELVRTGSPHVVCTVL ------------------PVDTSPYLTPENLIERTVDVLLAEHPGELVKTGSPHVVCTTL ------------------PEG----PLGMD--INAMHETLQACHGDLVRTGSPAILCSAL HSSSTASNNNNNGASNSNSNNNNAVHQDLLWMERLVQKRQQEHPGELVRTSNPYFLCSAL ---------------------NNQ---DIWWTEHLIHEIQTEHPGELVRTGSPYFLCSAL ---------------------------DLWWIERMVMEAQQEYPGELVRTGSPYFLCSAL ------------------------ILETLELISQGEWRIGGLTFLVAVKTGSPDYMCSIL -------------------------LTAESLAERTIEGLLADHPGELIRTGSPHVVCTVL ------------------PHASPAV--RMADVYAHIHEYYRQSHGELVQTGSPAVLCSAL -------------------------LTAELLAERTLEGLLADHPGELVKTGSPH-VCTVL -------------------------MTSDILAERTLNDFLSEHPGELIRTGSPLFVCTVL -------------------------MTSDILAERTLGDFLSEHPGELIRTGSPLFVCTVL ------------------LADAYTKMTSDIFAERTLGDYMSEHPGELVRTGSPHLVCTVL -------------------------LTAEMLAERTIDGLLAEHPGELVRTGSPHVVCTVL ----------------------------MADMFSNLHDMLQEYHGELVQTGSPAVLCSAL ------------------PEG----PIGMENHFSAIHDALRACHGELVQTGSPAILCSPL ---------------------EAAAVGELWWTERLVGEAQAEHPGELVRTGSPYFLCSQL ------------------LHETYTKMTSDILAERTLGDFLSEHPGELVRTGSPHFVCTVL ------------------NNLPDYALTADLLTERTLDGLFAEHPGELVRTGSPHVVCTVL ---------------------EGAAVGELWWTERLVGEAQAEHPGELVRTGSPYFLCSQL --------------------QRNKASSVFKGGERSIVDALSEYPGELVKTESPNFACSVL ------------------PINAQQP--SIMDMYASLQETLQEYHGELVQTGSPAVLCSVL ------------------PQNSAKMPSSMTDMFASLHEMLQEYHGELAQTGSPSILCSAL ------------------LNEAYTKMTSDILAERTLGDFLTEHPGELIRTSSPLFVCTVL

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

113 149 136 40 301 69 94 50 83 44 67 36 36 140 94 33 42 134 62 115 204 83 44 132 125

PAHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELRNSTALMKNQVAKFNDLR PSHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELRNSTALMKNQVAKFNDLR PTHWRSNKTLPIAFKVLALGEVMDGTIVTIRAGNDENFCGELRNCTAVMKNQVAKFNDLR PSHWRSNKSLPVAFKVVALDDVSDGTLVTIRAGNDENCCGELRNCTAVMKNQVAKFNDLR PAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLR PTHWRSNKTLPVAFKVVALGDIGDGTIVTVRAGNDENCCAELRNSTAVMKNQVAKFNDLR PNHWRSNKTLPSAFKVVSLGDVCDGTMVTIRAGNDENFCGELRNCTAVMRNQVAKFNDLR PQHWRSNKTLPGGFKVVALGDVLDGTLVTVRAGNDENCSAELRNNSAVMKNRVAKFNDLR PNHWRSNKTLPVAFKVVALGDVGDGTMVTVMAGNDENFCGELRNCTAIMKNQVAKFNDLR PGHWRSNKSLPLAFKVVALDDVQDGTLVTIKAGNDENVMAELRNCTAVMKNQVAKFNDLR PPHWRSNKTLPVAFKVVALGDVGDGTLVTVRAGNDENCSAELRNCTAVMKNQVAKFNDLR PPHWRSNKTLPVAFKVVALGDVGDGTVVTVKAGNDENYCAELRNSTAVMKNQVAKFNDLR PPHWRSNKTLPVAFKVVALGDVGDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLR PAHWRSNKTLPVAFKVVALGEVGDGTVVTVRAGNDENCCAELRNSTALMKNQVAKFNDLR PPHWRSNKTLPVAFKVVALGDVGDGTVVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLR PTHWRSNKSLPCAFKVIALDDIQDGTVVTIKAGNDENCHAELRNCTAIMKNQVAKFNDLR PSHWRSNKSLPVAFKVVALDEVSDGTLVTISAGNDENFCGELRNCTAVMKNQVAKFNDLR PTHWRSNKTLPAAFKVVALGEIGDGTLVTVRAGNDENCCAELRNSTALMKNQVAKFNDLR PPHWRSNKTLPVAFKVVALGDIGDGTLVTVRAGNDENCCAELRNSSAVMKNQVAKFNDLR PAHWRSNKTLPVAFKVVALGEVGDGTLVTVRAGNDENCCAELRNSTAVMKNQVAKFNDLR PTHWRSNKTLPVAFKVVALGEVVDGTLVTVRAGNDENCCAELRNSTTLMKNQVAKFNDLR PNHWRCNKSLPVAFKVVSLGETKDGTMVTIAAGNDENYCAELKNNTAVMKNQVARFNDLR PSHWRSNKSLPIAFKVVALDEVRDGTTVTLKAGNDENYCAELRNCTAVMKNQVAKFNDLR PNHWRSNKSLPGAFKVIALDDVPDGTLVSIKCGNDENYCGELRNCTTTMKNQVAKFNDLR PPHWRSNKTLPVAFKVVSLGDIMDGTMVTVRAGNDENYCAELRNCTAVMKNQVAKFNDLR

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

173 209 196 100 361 129 154 110 143 104 127 96 96 200 154 93 102 194 122 175 264 143 104 192 185

FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGRSYVREIIQY--------------------------------------FVPGKS FVGRSGR--------------------------------------------------GEFVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGRGAHWALSRLWARVPHLTVRVYRRSGVGESVTRTPPPPPAATGWYEPGSRSGKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR------------------EMYLRN----------------------SIVTGKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS FVGRSGR--------------------------------------------------GKS

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

183 219 206 110 371 139 164 132 152 114 137 156 106 210 164 103 112 204 142 185 274 153 114 202 195

FTLTITVSTTP-PQVATYTKAIKVTVDGPREPRSKTR----------QQQQFHAFAFASQ FTLTIMLQTSP-PQIATLSKAIKVTVDGPREPRSKTR-----------HQAFHPFHFGPR FTLTIVISTNP-IQIATYTKAIKVTVDGPREPRSKVR-----------HQGFHPFAFGPQ FSLTIQISTVP-FQVATYTKAIKVTVDGPREPRSKSN-----------------YQYGHFTLTITVATSP-PQVATYAKAIKVTVDGPREPRSKTSPT--------GGPHYRALGLGQR FSITITVSTTP-PQVATYTKAIKVTVDGPREPRSKTTG---------QHTAYRAIGLGQR FTLSITVSTTP-PQVTTYTKAIKVTVDGPREPRSKTNTP--------QLRPPPLHRFLEQ FSLTITISTNP-PQVATYQKAIKVTVDGPREPRSKTKQT---------CAQVRGAWVADG ---------------WNFSSSIYVTIY--------------------------------FTLTITISSFP-SQVATYTKAIKVTVDGPREPRTKQN-----------------YGYGHFTLTIMLATSP-PQVATYQKAIKVTVDGPREPRERVQ-----------ALGVSSLDAPCR FTLTIMVSTSP-PQVATYNKAIKVTVDGPREPRSKTR----------QQQQFH-FAFG-Q FTLTITISTSP-PQVATYNKAIKVTVDGPREPRSKTR----------QQQQFH-FAFG-Q FTLTITVSTTP-PQVATYAKAIKVTVDGPREPRSKTR----------QQQPFHPFAFASQ FTLSIIVSTTP-PQIATYNKAIKVTVDGPREPRSKTR-----------QQGFHHFPFGPR FSITITISTYP-CQIATYTKAIKVTVDGPREPRSKQN-----------------FAYGHFSLTIQISSVP-FQVATYTKAIKVTVDGPREPRSKSN-----------------YQYGPFSITITVSTTP-PQVATYTRAIKVTVDGPREPRSKTNALLDLVPGQ-QHQQFRALGLGQR FTLTITVSTTP-PQVTTYNKAIKVTVDGPREPRSKTMLSLLG-----QQQQFH-FAFG-Q FTLTIMLQTSP-PQVATLSKAIKVTVDGPREPRSKTR-----------HQAFHPFHFGPR FSITITVSTTP-PQVATYTRAIKVTVDGPREPRSKTRQTHIPGLAR-VPALPRVPGLTRG FTLSIFIYTNP-PQIATYNRAIKVTVDGPREPRRPKPKDQESRLMPPPIINTG------FTLTITISSEPYYQIATYNKAIKVTVDGPREPRTKSN-----------------YQYGYG FTLTITIATYP-VQIASYSKAIKVTVDGPREPRSKQS-----------------YGYPHFTLTITVSTNP-PHIATYNKAIKVTVDGPREPRSKTR----------QQQQFH-FAFG-Q

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145 GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

232 267 254 151 422 189 215 182 155 185 203 153 259 212 144 153 262 194 233 332 205 157 243 242 289 294 285 172 463 210 245 205 179 219 237 182 316 243 168 174 293 227 265 361 233 185 267 268

RGGPFFASPLVDPLQPLPNPLQPLPNPLQPRDPLSSFRHAMPANCQNMSQFGLTASN--------------------------PFPFGHPQDPLGFKLS------GLQHLGLDQGA--R-----------------------FGPDPL---MAGLPFKLPGFAHHLVGMHSHLHA-------------------------------------GFPGLGLLNPWVDVAYLGHAW---PYIDGFPS-------------------TKALHELESLRRSAKVAAVTTAAAAAATAASAA PFLDG------------------------------SFSTHLRDLEAYKKQR--------PAFSHFP---------------------------ARFNSSANKSSINTYSTSASHHQ--GIGRPSE----------------------------TVPAAARRSVGDLNLG----------------------------------------------------------------------------------------------------PGPFSPFLLNPGWLDAAYLNYAWA--R-----------------------WIIKLQRFDTGGIETNEITGELARKGAETSSAG--R-----------------------PFPFAASDPLSGFRMPPIGNCNNMPQFGLSSTN--R-----------------------PFHFPA-DPLSSFRMPPID----MSQFGLGTAN--RGGPFFASPLVDPLQPLPNPLQSLPNPLQPRDPLSSFRHAMPGNCQNMSQFGLTAGN--T-----------------------FAPDPL---AGSLPFKLSVGQRSRAAGDKATEV-----------------------------------PGAFNPFMLNPGWIDAAYMNYAWS-------------------------------------GFPALGLLNPWLDAAYFSHAW---PYLDGPN----------------------------SFTNHLRELE-YRRSKHHPHHHHHH R-----------------------PFPFPP-DPLGGFRMPPITTCQNMSQFGLSSSN--------------------------PFPFGHPQDPLGFKLTDPLDCTGLQHLGLEQG---TFPDAPS----------------------------PFTPYLRDSEPYRRNKHHVGNN-------------------------------HPHPFGEINPHHPNHHIGRQQSYQNQGR--L-----------------------------PGMPAGFNPFLLNPGWLDAAYMSYTWP-----------------------------------PGAFNPFMLNPAWLDAAYMTYGYA--R-----------------------PFHFST-DPLSGFRMPPIG----NCQS---ASN-----------------------------------S-WGYGSTA-GYAGYLPG-PLSSCAAQ ---------------------------------AAAAAAQGWGGLSRGGLPPHCLPPPPG ---------------------------------PDWRAHMALGGRPAAFTAAPFFGHHAA ----------------------------------HLPHPAFVKG--------------TI NAVAAAAAAVAVTPTGGGGGVAAGGVAGGAGAGLVQQLSSNYSSPNSTINSDCQVYKPNA ----------------------------------------GGPSSQS--------SDGSQ ----------------------------------------NSNSGSSSLNTSVESSSDYK ---------------------------------------VTTKLRPRKLSRQPVYYHPAY --------------------------------------------------------------------------------------------DYFRPPQMREPSTLIKGGAAPLTTPPV ---------------------------------LEPSTSTINIHWHPNSIGGSPLNADGS ---------------------------------SHWGYGAAG-AYSPYFTPSTLGSCAAP ---------------------------------SHWSYSSTG-PYSPYLTS-----CATP ---------------------------------S-WSYGSTA-GYAGYLPG-PLSSCAAQ ---------------------------------MSERVDSKTPHWS------------GG ---------------------------------DYFRQHQQLQAQQQASQPNPVTAAGKG ----------------------------------HLPHPALAKG--------------SI QQQQQQQQL-----------ATAQLQSPVSGDNANVNSSTTSPSSGSHLTNTTGGATAHQ ---------------------------------THWGYGGAS-AYPAYLP-----SCAAP -------------------------------------ASQGWGGLPRGSLPPHCLPPPPG --------------------VTANSVSGACT-NPNNTDPTASPASSAHLGPNSG-SSAHQ -----------------------------------MPRSYPLSPTSGSYDNIQHQGQASK ---------------------------------DYFRARTNVPTQTNLH---------AS ---------------------------------DYFRHQAAAQAAQVHHPALAKSSASSV ---------------------------------THWGYGSAASAYSPYLASSGLSSCTTP

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

313 321 312 184 523 222 265 226

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

353 368 361 211 566 247 297 236

206 246 263 203 340 258 195 186 342 248 288 399 258 203 294 295

239 295 307 247 387 298 238 218 402 288 348 437 301 236 326 340

ASFPPPPPPPPPPPP-----------------SSSSLASFAGAASMN-TPTAPDSTAG-HHSHAHPAAAAAAAA-------------VPQPTTAAAAADGTATAATAAAAAVAASAFNP AFPTASGLRGLSGDSQ-----------QHQQQQQQHQLATVGAAHSTTSPEGSPTTTTTS PMPSTDLFPPT---------------------FPPSVLPS--------YPF-DH-VKY-PHIQAAEMMGAGEWTNGS-----------------SSSAAAYYHSHAHHPHAHHAHAHLQ SSYKQDSQDGSL-----------------------HPNPPPCPPATWP-----------PNLTLPDYSNATDWPNGL-----------------NSSSTSAYVSNYSP----------FCPKAVMRFG------------------------------------------------------------------------------------------------------------TIPGADLFPFP---------------------PAVTNLPPGG------LIPPPGAFLPPN EETLLLGLYNRFYYAS-----------SLKVGGGIHGLRLPLAYQVDLEPVQRKNNMNSV TASQFNTPALGFSGS---------------TPDQTSTQDAF-GSTSNVTSLLPDTSTTDL TAAQFNNPALGFTCS---------------SGEQNAGQDFT-STGRDCVPMLPDSTAADL TPFAAPPPPPPPPPTG-------------ISSTGSALTSFTGGTAMNNTPAAPDSTAGNN AASDLIGIAHHL--------------------AGLPGPPPEWAMLGGRHPYPPGPFVPHH RNSSNTQLFSSSLFL-----------------AGSPTLPTPNGTTGPLLPTPPADFMSLS GMPHTDLFGPS---------------------FPPTVLPTG-------YPLTDHNVKYNS DCYKHSPQHGDTSVGTGGGGGGGSNSGATTATEWAYPSAAPSYPAPAGSAAGGAATAGGG AASQFNPPALGFAGT---------------VPDQTATQDFT-ANN----TVLPDTTGVDL HHSHTHPPAAGASAFNPFHHSIEQRSPRLPGPNSEPSRNDLGPISVSVREVTPTTSPGNP DCYKHSPQHGDT--GTAG------------SAEWTYPSAAPSYPAPPVSSGG-------PWSYYNPYQSSVAQLS-----------------DTSILSAQIKTEPTELALLGQQNSTLQ LIKGATQLPPT---------------------NGEFYLPPQP------QFHPPHNFLPPN SPNPNPSVATS---------------------SSSAVQPSE-------YPHPAAAVAAAA TSAQFNNPALGFTCS---------------SNDQSNNQDFGGATNRDCVPMLPDSTASDL ---------STVTS---------------------------------------------FHHVAAAAAIEQRSPRLPATAN---------------GLHLLGATNSLFGSIFAPLLPQS GTQLSAFVQPPMTSSPPPVTSLQH-----------------------------------D -----------------------------------------------------------HQMALPPPPPPPAAAPVSVGVGGN--------------------------GATMGMGMGV ----DYSYTP-----------------------------------------------------LQTTPPS----------------------------------------------------------------------------------------------------------------------------------------------------------------------G------LLPFPPH---------------------------------------------ALYFGSSSWAKDRGYICDLGHSSL-----------------------------------V DQHLG-LVSSQNHTNHSQ-----------------------------------------DQHLSSLVGTQQTAQMTHPSLM----------------TTNGSSN--TPGNGTNGNGVTS GPATVPVPVANAAT---------------------------------------------HPHFPPHMFA---------ALDRP-----------------------------------I GAQPTPNGLPTPTG---------------------------------------------T------------T---------------------------------------------SGSGSYSPIPAGA----------------------------------------------DQQLSGLVGS-------------------------------------------------GPGLLTTATVAAPTPPAEPTTTTTTPSPSSGHHSHFQGLHLLGATGSIFGSIFAPLLPQS ----SFSPIPGGA----------------------------------------------QYPKPDSLYPTSITR--------------------------------------------G------ILP-------------------------------------------------G-QPAA-MMPSPPG---------------------------------------------DQHLSSLVG-STSGQMTHHSLLG------AGGQTSISSTVNGASGGGSAGAGTAGGGAGS

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145 GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

358 413 386 211 600 253 304 236 247 320 324 289 401 314 252 220 415 298 408 446 316 240 338 393 409 472 446 239 655 291 350 286 288 379 368 344 453 359 306 261 465 341 468 496 371 263 391 446

------GTGAGSTAQQDAFSAVSSLVPDSTTTG--QSDPLDPLSSLMSGT-PSQRYQDYV SWLYNPLYHTQQYILEPEWHALALRMAQQRLQRPDQARLEDLRKLRGSSASPEDVSKDKNNNNNSNNNNSSSHIDAGFESDSISVTGSPRKSLGSPLTHDEEEAEAEAEAEAEAEAEAE ------PAEYATTLPPKSS----------SSSSAQAT---IPTSPSR------------GVGMGMNHYGGGYDSANSLEAGQYAAHLPAVLPEMHG--HGFATDP---YQTAGYGGGNT -----YSPQTGYYDHQDSATS----LHLPTVLPEP--GHNEFIN-----------TSLTS --------YGG-YDPCPMAEHPNYN--LPAVLPDTAQTYQDYYSIP---PASGSTPHQAM --------LKGGAAVVTILETLELTSQVLPELPLGHADYSGFQSSTTS--YKGSPSGTSS -----------------------------------------------------------------PAELALKSLPPELSLKSGLTPYEALRQFQNNVSSMDTSSAR------------GWSTWRRMGSASPALPP-TNSASTSALHSTRAASSAYTAPRTPTASRKRVLKFLIFTDVT ------SSTHTSSLLVPRYST--NHSDFGLS----GPRSLSDNSSAAESP----VQDDIL QANSITTTNGSNGLLVPRYQSNSSNNDYTLHTSQSGPRSLSD-SSQAESP----VQEDLL ------ANANNPAAQQDAFSSVSSLPDTTTPGV--QPDPLDHLSSLMSSTGSAQRYQDYV NTSPRIANEPSSTSLGP-ISINCTPAHSTTSPKTSPTQVGLLTTAG-------------------PVLPNGATYLPQFPFGPHADLHLKSPFLPYDISPLRANGLRGPHQLSATITLSS ------AADFASSMSHKSSSIHSMTPPPTTTPTAPPTSLLLPNSPSR-----------------FSYPGEALAHHPTTEP----VPLPTVLPSD-GQQNAYTPNCVSMYPSGAASSTTS ------SPSHHG-TLLPRYN---NNADYSLST---GPRSLSDNSSQPESP----VQDDLL SWLYNPLYHTQQYVLEPEWHALALRMAQQRLQRPDQARVEETRKVRAGSSSPESALKEAK -----FSYPGEALSHHPTTEP----VPLPTVLPSDTSQQDTYTPNCVSMYPSHGASS-TS -----SSEVQDPRFVYPSTPAAVSSVSFTPSSMSVLSSGVESPRTILPMTPNPFPLSSQD --------QLSLSDSP----------------LLRPSVGPMDQLSLR-------------------AAPATPYAIPQFPFNHVAAAAAAKAATPHAFHPYNFAAAAGLRARNAALHHQS GGGAGGGAGG-NSILVPRYHTNASN-EYNVHSSQNGPRSLSD-SSQAESP----VQEDLL SPRSLSTDS-----------------------------------------------STTE ----EEASRD---------------------------------------------LDSPD EAEVGGLSRNGGGIQ-----------------------------------GPLHSESSPG -----------------------------------------------------------GGGSASKS----------------------------------------------ELDYGG PPPMLNSAK----------------------------------------------SELDP SHGYVSKTT---------------------------------------------DLDTSY SLTELNPTTPVTTQR---------------------------------------YDSNYY ----------------------------------------------------------------------------------------------------------------------SDNPISMRAHSTTSP-----------------------------------ANSRPCSPQD S----TQST-LG----VNHVN----------------------------------NTNFP S----SNTPNLG-----GAAN-----------------------------------QNFS SPRSLSTDS-----------------------------------------------SATE -----------------------------------------------------APLSPPE SD------------------------------------------------------FISS -----------------------------------------------------------SLALIQNPSSVKPLV---------------------------------------DTSSDM T----SNTTNIG----HNHSN----------------------------------TSNFP TRRDDEDNRDPARVRSG--------------------------------------PDSPD SLPLVPAKS----------------------------------------------SDPDI IFSSSSTATP---------------------------------------------VTLTS -----------------------------------------------------------E----------------------------------------------------------P T----TNTPNLGSTAGGGAANGGAGSNAGSGAAGSGAGGAGGASSAVGNPAMLGANQNFP

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145 GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

422 483 471 239 669 305 365 307 288 404 385 360 466 366 312 261 486 359 490 510 386 263 393 502 448 514 518 257 710 345 403 345 318 450 412 390 495 408 341 279 545 386 520 566 420 279 422 549

SPVHE-EQGFGQNY---------------------------------GNYFPTPGVLPSI GSIEVDDSEEPRPKIERR-----------------------------SQSEESLEQDSSP SGGAFTALIQRSGKNPTELFGG-------------FAAAGGNHFAPSGHSFNPALAAQLF --TPPKSPSESGS----------------------------------------ESAPEEV SYNQAWSNGYQNYQYGSCLAT------------AQYGPQAA-------PPPQPPPPPPVV MRYSDNST-----YYPNNWTP------------NSYPNNYN-YYNT--PNNNQQYPSTMV NPYSQWANGYGSYQYNAPCPP------------PLPPPQ----------PQYPSTAPAMV NSWPANSYNYNNYQYNNINNN--------PACIQSHAP--------------YINPNPQM -------------------------------------------------------------LSPTSSRQSGSPRSMA----------------------------NASPDRSKTDSKSE DDISVTASMSPPPDERPGAFT--------------RPRKPLQPLPAPSSLFHSALAAQLF LHQNMTSSSYPSSN---------------------------------CNNSIYPVLPASL SLVVNQSQSNYGASGG------------------------------SCNGSLYPVLPASL SPVHEQDQSFGQNCSA-------------------------------GNYFPAPSVFPTI DDISVTASPTPSPQPPPAFPG--------------APPPPIQ----NNQLFNNALAASLF SRLSPASSRNSTSSPP-------------------------------STLNQSQIKMSIE --TPPRSPSDSGS----------------------------------------ESAPEEV YATATGSPGSTGYHHYGGWTTAPGPATPVPAPAN-YNPNYQGYYHQNPAAQNYIGQAPMV SLMGSQNSSYGSSN---------------------------------CNNSLYPVLPASL GSIEVDDRNEQESGKDR------------------------------ARSDESVGGQDSP FAGGGTGSGSPGYHYGPSWTS--GPATPVPVASHNYNPNYQGYYNN--PSQNYISPAPMV PPYLPNSPPYPLYPHLYMSSP--------------------------SSQTYYDSSHLPM --ISPVSNSQS------------------------------------------PTAQETK VHVSPASSRPSSSSPT-------------------------------QQHVLLKLNTSIE GIVNQAQHSAASAYGGGTGVVG-------------GGHGSAAAAAAGCNGSLYPVLPASL LYSQLYGNQFQ---------------------NSES-----------------------RTSPVRTDTELT-------QEETSSRLRPSLENVNEL---------DNSTTQLDHQLDQQ LQSPLLPQSSQWLYTQLYGSYSDLPWLRNAAAAAAANINPGQENSGIPPLGSNPDHDGVN R-SAFVPIRLNT------------LPPTTSVVTATS---------------------ASS LCPQLYS-TVNQNQIHLHLHS---SEKLEQYLGTAT----------------------SA LYPPLYS---TQNQIHFHLHGS--SEKIDQYLSTES-----------------------LCPQMFS-TVNQNQIHVHLHGTG-SEKLEQYLGSENGF--------------------SI ILPNLYS-TVNQNQIHVHLHSSSDKYNLEQCIPSEIK---------------------------------------------------------------------------------ANSIHDATITD-------------ESDEEPIEVVKS---------------------AFH LNSPLLPTPPAWLYSQLYGG---------YDWWLRP----------PAPQE---DPSSSP LYSQLYSAANQ--------SHNFHSLHS---HTTQS-------------HHNDLQTVMDQ LYSQLYTAANQ--------THGFHNHPLQNSHSTSQN---------ASMHAGELQSVMDH LYSQLYNNQFQ---------------------STEA-----------------------LNAPLLPPPGQWFYSQFYPHDWAWMNLRHHSLLPRS----------SSPLELGHNPNGST LNSTNEHSSGE-------------ESDDEHIDVVKS---------------------AFV VRSAFAPIRPNS------------N--NNNIHVPTS---------------------SSS LYPQVFTSTINQNQIHLHLS--------EETLANSN-----------------------LYSQLYTAANQ--------THNFHPLHSNSIHSTQN-------------HHNELQTMMDQ RISPVRSDTEDTTADATAFQEDGTAHLRPSMENSNLD---------QEVLATDGHQVSRR LYPQLYS-TVNQNQIHLHLHGD--LSEEQVTIAGSN-----------------------LPSSTRPEDKQEIKHDNRPGEGIPHIPHPEMSLTVALN------------------YGSH IHSTVDQSTSE-------------NSDDEDIDVVKS---------------------AFV TSSIHEQSASDG------------DSDDEQIDVVKS---------------------EFD LYSQLYTAANQS-------AHGFHSHTLP--AHASPN---------SSVHG-ELQSVMDH

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

463 558 578 283 744 376 441 381

GB19482 Nvi538857 CG42267 GB11654 Dmlz Tc05796* AAEL007040* BGIBMGA008907* AAEL007036* BGIBMGA008906 BGIBMGA008905* Tc05566* AAEL006160* Nvi538861 Tc06536 AAEL006167 Nvi541634 Nvi541690 BGIBMGA008970* GB15836 GB16431 SpRUNT1 Tc06542 Dmrun CG34145

481 617 638 328 804 411 496 417




344 488 448 433 510 458 367 304 573 425 571 599 462 305 449 590

402 527 493 490 532 512 426 354 595 470 619 621 522 361 502 646

---------PEQ---------------RAVADS-CSVR-QEEVR---------------PASRGLTVKIRVEG-TVDLSTKTRTHHQDKENVGQDLTTTTRRKDDDVEISEREAIRPRR LIKRCVTLITHNPPDAENANPNASPPVSSTRRSPSPVETIDLDDVSTTSRSASGSSGHGG PERLP--TKKG--VVVEGSRNELKAP--------TALISRSMS--PKRSPSPTKIS-SPP DHLTIGSLTGSSRSSIEIGQDQYHQQVHHAQQQQQQQQQQQQVHHPQQQQVESAGEVGGS -------LSLTPTRAIELAQTVTTEVAQSHETLEDTERTHDP-----------------NSISSGGIRPSAVPGIEIGIATHDSSLVSNDGSHQAQQQPQ-----QQEQQHSAVEVSEQ ------ISDIDGGISITTELQGTGEPSGIVQTCEANDEVKHG----------------------------------------------------------------------------PTRPANLELQEMKRVQAADSTVSDRPRTRNELKSTSQRTTRVLS--TSPTSTKIANGTIP DRE----EESSTPSVAGKKRP------ASPEWSDQSVR----------TRSKSLTTPEKLS-------TSNQRQMNGST-----DLLLSNNGTCAAAAARQD---DARLTSNGAQRGPG LTSSTANGGRQHPNLMTNN--PHT-DLSLIGNCGAMARGALEEPGARALAGGRGVPPQGQ ---------QEQQH-------------RALADVSCSVRVQEEVR---------------KSEGVDDEVKSDDGKAKEEKK------GKDEGVNLSLHRCRKAAITLVRQKEEVSSSEKE PILRPAPTSSPPPPAPPSARCELKAPSSKKPQFHESAPSEPASPENTKLKSPNLIIKQSPDRLS--NNNNSKRPIEGTRCELKAP--------KALISHRTKTLGKRSPSPTKIQPTTP -------LTISSNR-LEIGVLG-----------EENEQRND------------------ISS------TTNNHRQHGNQ-----DLLLGGSSSCAAAAARGE---DGRVN-NLGQRGNP ES------KIRLEVGTVDLSTKNK--GQDKENVGQDLTT--KRKEEDTER--EAVVRGRR -------LTISSNR-LEIGVMG-----------EENEQRND------------------PQSQIELNTARTMGQSMGGMHNSGVALMQQHRNHSPVTNISPMQQSMGQMNINTTVGHLP PIKPANLILQEVQ----PDSTVQDKELKKNELKAPSSRSIKSLAEEKSPNTKIHHQQEIT -------LDKSLDVAPLRMRCDLKAPSAMKPLYHESGPGAVANSRQPSPETTTKIKSAAV ISN---VGVRQQHNIMAGGGVTHPGDLTLIGNCGASVR-NIEDGNSNRQVAALAAHRGHH --------------PDNN---VWRPY--------------ERSPKPRQVWRPY-GVGGGGAVGPIRTRTPKPSADVWRPY------------------PAKPVWRPY-GAGG---VESAREEDVGDLSQVWRPY--------------------ASVWRPY--------QDSREEAVTGDPSSVWRPY--------------MYGAGSQEVWRPY---------------------------------------------SHKSVWRPY------------------RPVDVWRPY--------------QNSDA--VVWRPY--------------VQSENGNSVWRPY---------------PDNN---VWRPY-------------HLKCNRLGDVWRPY-------------------AKTVWRPY------------------AVKTVWRPYPC ---------------------VWRPY--------------QPDSN--TVWRPY--------------ERSPKPRQVWRPY----------------------VWRPY-------------TVDDSRKEDVWRPY------------------ATKSVWRPY------------------QQKTVWRPY--------------NPTDNGGSVWRPY--